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Yorodumi- PDB-5i2d: Crystal structure of T. thermophilus TTHB099 class II transcripti... -
+Open data
-Basic information
Entry | Database: PDB / ID: 5i2d | ||||||
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Title | Crystal structure of T. thermophilus TTHB099 class II transcription activation complex: TAP-RPo | ||||||
Components |
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Keywords | TRANSCRIPTION/DNA/RNA / Transcription / RNA polymerase / Catabolite activator protein / cAMP receptor protein / TRANSCRIPTION-DNA-RNA complex | ||||||
Function / homology | Function and homology information sigma factor activity / DNA-directed RNA polymerase complex / DNA-templated transcription initiation / ribonucleoside binding / DNA-directed 5'-3' RNA polymerase activity / DNA-directed RNA polymerase / protein dimerization activity / DNA-binding transcription factor activity / DNA-templated transcription / magnesium ion binding ...sigma factor activity / DNA-directed RNA polymerase complex / DNA-templated transcription initiation / ribonucleoside binding / DNA-directed 5'-3' RNA polymerase activity / DNA-directed RNA polymerase / protein dimerization activity / DNA-binding transcription factor activity / DNA-templated transcription / magnesium ion binding / protein homodimerization activity / DNA binding / zinc ion binding / metal ion binding / cytoplasm Similarity search - Function | ||||||
Biological species | Thermus thermophilus (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 4.405 Å | ||||||
Authors | Feng, Y. / Zhang, Y. / Ebright, R.H. | ||||||
Funding support | United States, 1items
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Citation | Journal: Science / Year: 2016 Title: Structural basis of transcription activation. Authors: Feng, Y. / Zhang, Y. / Ebright, R.H. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5i2d.cif.gz | 1.6 MB | Display | PDBx/mmCIF format |
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PDB format | pdb5i2d.ent.gz | 1.3 MB | Display | PDB format |
PDBx/mmJSON format | 5i2d.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/i2/5i2d ftp://data.pdbj.org/pub/pdb/validation_reports/i2/5i2d | HTTPS FTP |
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-Related structure data
Related structure data | 4g7hS S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
-DNA-directed RNA polymerase subunit ... , 4 types, 10 molecules ABLMCNDOEP
#1: Protein | Mass: 35056.164 Da / Num. of mol.: 4 / Source method: isolated from a natural source Source: (natural) Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria) Strain: HB8 / ATCC 27634 / DSM 579 / References: UniProt: Q5SHR6, DNA-directed RNA polymerase #2: Protein | Mass: 125436.539 Da / Num. of mol.: 2 / Source method: isolated from a natural source Source: (natural) Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria) Strain: HB8 / ATCC 27634 / DSM 579 / References: UniProt: Q8RQE9, DNA-directed RNA polymerase #3: Protein | Mass: 170997.391 Da / Num. of mol.: 2 / Source method: isolated from a natural source Source: (natural) Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria) Strain: HB8 / ATCC 27634 / DSM 579 / References: UniProt: Q8RQE8, DNA-directed RNA polymerase #4: Protein | Mass: 11533.316 Da / Num. of mol.: 2 / Source method: isolated from a natural source Source: (natural) Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria) Strain: HB8 / ATCC 27634 / DSM 579 / References: UniProt: Q8RQE7, DNA-directed RNA polymerase |
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-Protein , 2 types, 6 molecules FQGHRS
#5: Protein | Mass: 50769.398 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria) Strain: HB8 / ATCC 27634 / DSM 579 / Gene: sigA, TTHA0532 / Production host: Escherichia coli (E. coli) / References: UniProt: Q5SKW1 #6: Protein | Mass: 24344.762 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria) Strain: HB8 / ATCC 27634 / DSM 579 / Gene: TTHB099 / Production host: Escherichia coli (E. coli) / References: UniProt: Q53W63 |
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-DNA chain , 2 types, 4 molecules ITJU
#7: DNA chain | Mass: 22234.168 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) Thermus thermophilus (bacteria) #8: DNA chain | Mass: 22341.262 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) Thermus thermophilus (bacteria) |
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-RNA chain , 1 types, 2 molecules KV
#9: RNA chain | Mass: 1240.802 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) Thermus thermophilus (bacteria) |
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-Non-polymers , 2 types, 6 molecules
#10: Chemical | ChemComp-ZN / #11: Chemical | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.16 Å3/Da / Density % sol: 61.08 % |
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, hanging drop / pH: 7.6 / Details: 0.1 M HEPES, pH 7.6, 0.1 M MgCl2, and 6% PEG4000 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: NSLS / Beamline: X25 / Wavelength: 1.1 Å |
Detector | Type: PSI PILATUS 6M / Detector: PIXEL / Date: Apr 26, 2013 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.1 Å / Relative weight: 1 |
Reflection | Resolution: 4.4→49.31 Å / Num. obs: 71660 / % possible obs: 98.7 % / Redundancy: 5.9 % / Rmerge(I) obs: 0.187 / Net I/σ(I): 8.182 |
Reflection shell | Resolution: 4.4→4.48 Å |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 4G7H Resolution: 4.405→49.31 Å / SU ML: 0.6 / Cross valid method: FREE R-VALUE / σ(F): 0 / Phase error: 37.37
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 4.405→49.31 Å
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Refine LS restraints |
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LS refinement shell |
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