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TitleShulin packages axonemal outer dynein arms for ciliary targeting.
Journal, issue, pagesScience, Vol. 371, Issue 6532, Page 910-916, Year 2021
Publish dateFeb 26, 2021
AuthorsGirish R Mali / Ferdos Abid Ali / Clinton K Lau / Farida Begum / Jérôme Boulanger / Jonathan D Howe / Zhuo A Chen / Juri Rappsilber / Mark Skehel / Andrew P Carter /
PubMed AbstractThe main force generators in eukaryotic cilia and flagella are axonemal outer dynein arms (ODAs). During ciliogenesis, these ~1.8-megadalton complexes are assembled in the cytoplasm and targeted to ...The main force generators in eukaryotic cilia and flagella are axonemal outer dynein arms (ODAs). During ciliogenesis, these ~1.8-megadalton complexes are assembled in the cytoplasm and targeted to cilia by an unknown mechanism. Here, we used the ciliate to identify two factors (Q22YU3 and Q22MS1) that bind ODAs in the cytoplasm and are required for ODA delivery to cilia. Q22YU3, which we named Shulin, locked the ODA motor domains into a closed conformation and inhibited motor activity. Cryo-electron microscopy revealed how Shulin stabilized this compact form of ODAs by binding to the dynein tails. Our findings provide a molecular explanation for how newly assembled dyneins are packaged for delivery to the cilia.
External linksScience / PubMed:33632841 / PubMed Central
MethodsEM (single particle)
Resolution4.3 - 9.32 Å
Structure data

11576

6zyw

EMDB-11576, PDB-6zyw:
Outer Dynein Arm-Shulin complex - overall structure (Tetrahymena thermophila)
Method: EM (single particle) / Resolution: 8.78 Å

EMDB-11577:
Outer Dynein Arm-Shulin complex - full tail region (Tetrahymena thermophila)
Method: EM (single particle) / Resolution: 6.66 Å

EMDB-11578:
Outer Dynein Arm-Shulin complex - ordered tail region (Tetrahymena thermophila)
Method: EM (single particle) / Resolution: 5.92 Å

11579

6zyx

EMDB-11579, PDB-6zyx:
Outer Dynein Arm-Shulin complex - Shulin region from Tetrahymena thermophila
Method: EM (single particle) / Resolution: 4.3 Å

EMDB-11580:
Outer Dynein Arm-Shulin complex - extended Shulin region (Tetrahymena thermophila)
Method: EM (single particle) / Resolution: 4.63 Å

11581

6zyy

EMDB-11581, PDB-6zyy:
Outer Dynein Arm-Shulin complex - Dyh3 motor region (Tetrahymena thermophila)
Method: EM (single particle) / Resolution: 4.4 Å

EMDB-11582:
Outer Dynein Arm-Shulin complex - Dyh4 motor (Tetrahymena thermophila)
Method: EM (single particle) / Resolution: 5.03 Å

EMDB-11583:
Outer Dynein Arm-Shulin complex - Dyh5 motor (Tetrahymena thermophila)
Method: EM (single particle) / Resolution: 5.62 Å

EMDB-11584:
Outer Dynein Arm-Shulin complex - extended Dyh5 motor region (Tetrahymena thermophila)
Method: EM (single particle) / Resolution: 9.32 Å

Chemicals

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM / Adenosine diphosphate

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM / Adenosine triphosphate

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM / Guanosine triphosphate

Source
  • tetrahymena thermophila (strain sb210) (eukaryote)
  • tetrahymena thermophila sb210 (eukaryote)
  • Tetrahymena thermophila (eukaryote)
  • tetrahymena thermophila cu428 (eukaryote)
KeywordsMOTOR PROTEIN / Cilia / dynein / microtubules / motor / Complex

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