Main map of overall ODA-Shulin structure (resampled on a full-length ODA-Shulin structure from a smaller subset of particles using EMDA). Unsharpened map.
試料
複合体: Tetrahymena thermophila ODA Dyh4 motor
複合体: Tetrahymena thermophila ODA Dyh4 motor
タンパク質・ペプチド: x 16種
複合体: Shulin
タンパク質・ペプチド: x 1種
リガンド: x 3種
キーワード
Cilia / dynein / microtubules / motor / MOTOR PROTEIN
機能・相同性
機能・相同性情報
axonemal dynein complex / outer dynein arm / outer dynein arm assembly / dynein light chain binding / dynein heavy chain binding / cilium movement / dynein complex / minus-end-directed microtubule motor activity / dynein light intermediate chain binding / cytoplasmic dynein complex ...axonemal dynein complex / outer dynein arm / outer dynein arm assembly / dynein light chain binding / dynein heavy chain binding / cilium movement / dynein complex / minus-end-directed microtubule motor activity / dynein light intermediate chain binding / cytoplasmic dynein complex / motile cilium / dynein intermediate chain binding / microtubule-based movement / protein-disulfide reductase activity / microtubule-based process / cell redox homeostasis / microtubule / cilium / ATP hydrolysis activity / ATP binding / cytoplasm 類似検索 - 分子機能
Uncharacterized protein C20orf194-like / : / Dynein axonemal assembly factor 9 N-terminal domain / Dynein heavy chain 3, AAA+ lid domain / Dynein light chain Tctex-1 like / Tctex-1-like superfamily / Tctex-1 family / AAA+ lid domain / : / Dynein light chain, type 1/2 ...Uncharacterized protein C20orf194-like / : / Dynein axonemal assembly factor 9 N-terminal domain / Dynein heavy chain 3, AAA+ lid domain / Dynein light chain Tctex-1 like / Tctex-1-like superfamily / Tctex-1 family / AAA+ lid domain / : / Dynein light chain, type 1/2 / Dynein light chain type 1 / Dynein light chain type 1 / Dynein light chain superfamily / Roadblock/LAMTOR2 domain / Roadblock/LC7 domain / Roadblock/LC7 domain / Filamin/ABP280 repeat / Filamin/ABP280 repeat profile. / Filamin/ABP280 repeat-like / Dynein heavy chain, AAA 5 extension domain / Dynein heavy chain AAA lid domain / KLHDC2/KLHL20/DRC7 Kelch-repeats domain / Dynein heavy chain, C-terminal domain / Dynein heavy chain, C-terminal domain, barrel region / Dynein heavy chain C-terminal domain / Thioredoxin / IPT/TIG domain / P-loop containing dynein motor region / Dynein heavy chain, tail / Dynein heavy chain, N-terminal region 1 / Dynein heavy chain region D6 P-loop domain / Dynein heavy chain, linker / Dynein heavy chain, AAA module D4 / Dynein heavy chain, coiled coil stalk / Dynein heavy chain / Dynein heavy chain, hydrolytic ATP-binding dynein motor region / Dynein heavy chain, ATP-binding dynein motor region / Dynein heavy chain AAA lid domain / Dynein heavy chain AAA lid domain superfamily / Dynein heavy chain, domain 2, N-terminal / Dynein heavy chain, linker, subdomain 3 / Dynein heavy chain, AAA1 domain, small subdomain / Dynein heavy chain region D6 P-loop domain / Dynein heavy chain, N-terminal region 2 / Hydrolytic ATP binding site of dynein motor region / Microtubule-binding stalk of dynein motor / P-loop containing dynein motor region D4 / ATP-binding dynein motor region / Dynein heavy chain AAA lid domain / ig-like, plexins, transcription factors / Kelch-type beta propeller / IPT domain / Thioredoxin / Thioredoxin, conserved site / Thioredoxin family active site. / Thioredoxin domain profile. / Thioredoxin domain / Immunoglobulin E-set / Thioredoxin-like superfamily / WD domain, G-beta repeat / Trp-Asp (WD) repeats profile. / Trp-Asp (WD) repeats circular profile. / WD40 repeats / WD40 repeat / WD40-repeat-containing domain superfamily / WD40/YVTN repeat-like-containing domain superfamily / ATPases associated with a variety of cellular activities / AAA+ ATPase domain / Immunoglobulin-like fold / P-loop containing nucleoside triphosphate hydrolase 類似検索 - ドメイン・相同性
Dynein light chain / Dynein light chain tctex-type 1 protein / Dynein intermediate chain 2 / Dynein-1-alpha heavy chain, flagellar inner arm I1 complex protein, putative / Outer arm dynein beta heavy chain / Dynein light chain / Dynein light chain roadblock-type 2 protein / Dynein light chain 2A / Dynein heavy chain, outer arm protein / Dynein light chain roadblock-type 2 protein ...Dynein light chain / Dynein light chain tctex-type 1 protein / Dynein intermediate chain 2 / Dynein-1-alpha heavy chain, flagellar inner arm I1 complex protein, putative / Outer arm dynein beta heavy chain / Dynein light chain / Dynein light chain roadblock-type 2 protein / Dynein light chain 2A / Dynein heavy chain, outer arm protein / Dynein light chain roadblock-type 2 protein / Dynein light chain / Dynein axonemal assembly factor 9 N-terminal domain-containing protein / Flagellar outer dynein arm intermediate protein, putative / Thioredoxin / Dynein light chain / Dynein light chain / Dynein light chain 類似検索 - 構成要素
ジャーナル: Science / 年: 2021 タイトル: Shulin packages axonemal outer dynein arms for ciliary targeting. 著者: Girish R Mali / Ferdos Abid Ali / Clinton K Lau / Farida Begum / Jérôme Boulanger / Jonathan D Howe / Zhuo A Chen / Juri Rappsilber / Mark Skehel / Andrew P Carter / 要旨: The main force generators in eukaryotic cilia and flagella are axonemal outer dynein arms (ODAs). During ciliogenesis, these ~1.8-megadalton complexes are assembled in the cytoplasm and targeted to ...The main force generators in eukaryotic cilia and flagella are axonemal outer dynein arms (ODAs). During ciliogenesis, these ~1.8-megadalton complexes are assembled in the cytoplasm and targeted to cilia by an unknown mechanism. Here, we used the ciliate to identify two factors (Q22YU3 and Q22MS1) that bind ODAs in the cytoplasm and are required for ODA delivery to cilia. Q22YU3, which we named Shulin, locked the ODA motor domains into a closed conformation and inhibited motor activity. Cryo-electron microscopy revealed how Shulin stabilized this compact form of ODAs by binding to the dynein tails. Our findings provide a molecular explanation for how newly assembled dyneins are packaged for delivery to the cilia.
ダウンロード / ファイル: emd_11576.map.gz / 形式: CCP4 / 大きさ: 347.6 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
注釈
Main map of overall ODA-Shulin structure (resampled on a full-length ODA-Shulin structure from a smaller subset of particles using EMDA). Unsharpened map.