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- PDB-4ym7: RNA polymerase I structure with an alternative dimer hinge -

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Basic information

Entry
Database: PDB / ID: 4ym7
TitleRNA polymerase I structure with an alternative dimer hinge
Components
  • (DNA-directed RNA polymerase I subunit ...Polymerase) x 7
  • (DNA-directed RNA polymerases I and III subunit ...) x 2
  • (DNA-directed RNA polymerases I, II, and III subunit ...RNA polymerase) x 5
KeywordsTRANSFERASE / RNA polymerase I / dimer hinge
Function / homology
Function and homology information


RNA polymerase III activity / RNA polymerase I preinitiation complex assembly / RNA Polymerase I Transcription Initiation / Processing of Capped Intron-Containing Pre-mRNA / : / RNA Polymerase III Transcription Initiation From Type 2 Promoter / regulation of cell size / RNA Pol II CTD phosphorylation and interaction with CE / Formation of the Early Elongation Complex / mRNA Capping ...RNA polymerase III activity / RNA polymerase I preinitiation complex assembly / RNA Polymerase I Transcription Initiation / Processing of Capped Intron-Containing Pre-mRNA / : / RNA Polymerase III Transcription Initiation From Type 2 Promoter / regulation of cell size / RNA Pol II CTD phosphorylation and interaction with CE / Formation of the Early Elongation Complex / mRNA Capping / Formation of TC-NER Pre-Incision Complex / RNA polymerase II transcribes snRNA genes / RNA Polymerase I Promoter Escape / TP53 Regulates Transcription of DNA Repair Genes / Estrogen-dependent gene expression / RNA Polymerase II Promoter Escape / RNA Polymerase II Transcription Pre-Initiation And Promoter Opening / RNA Polymerase II Transcription Initiation / RNA Polymerase II Transcription Initiation And Promoter Clearance / RNA-templated transcription / termination of RNA polymerase III transcription / RNA Polymerase II Pre-transcription Events / Dual incision in TC-NER / RNA polymerase I activity / termination of RNA polymerase I transcription / transcription initiation at RNA polymerase III promoter / tRNA transcription by RNA polymerase III / nucleolar large rRNA transcription by RNA polymerase I / Gap-filling DNA repair synthesis and ligation in TC-NER / transcription initiation at RNA polymerase I promoter / transcription elongation by RNA polymerase I / RNA polymerase I complex / transcription by RNA polymerase I / RNA polymerase III complex / transcription by RNA polymerase III / RNA polymerase II, core complex / promoter-specific chromatin binding / transcription elongation by RNA polymerase II / transcription initiation at RNA polymerase II promoter / ribonucleoside binding / DNA-directed RNA polymerase / peroxisome / ribosome biogenesis / RNA polymerase II-specific DNA-binding transcription factor binding / transcription by RNA polymerase II / nucleic acid binding / protein dimerization activity / nucleolus / negative regulation of transcription by RNA polymerase II / DNA binding / zinc ion binding / nucleoplasm / metal ion binding / nucleus / cytoplasm
Similarity search - Function
RNA polymerase I, subunit Rpa14, fungi / Yeast RNA polymerase I subunit RPA14 / DNA-directed RNA polymerase I, subunit RPA34.5 / DNA-directed RNA polymerase I subunit RPA34.5 / RNA polymerase I associated factor, A49-like / A49-like RNA polymerase I associated factor / Rpa43, N-terminal ribonucleoprotein (RNP) domain / RPA43, OB domain / RPA43 OB domain in RNA Pol I / DNA-directed RNA polymerase I subunit RPA2, domain 4 ...RNA polymerase I, subunit Rpa14, fungi / Yeast RNA polymerase I subunit RPA14 / DNA-directed RNA polymerase I, subunit RPA34.5 / DNA-directed RNA polymerase I subunit RPA34.5 / RNA polymerase I associated factor, A49-like / A49-like RNA polymerase I associated factor / Rpa43, N-terminal ribonucleoprotein (RNP) domain / RPA43, OB domain / RPA43 OB domain in RNA Pol I / DNA-directed RNA polymerase I subunit RPA2, domain 4 / DNA-directed RNA pol I, largest subunit / Pol I subunit A12, C-terminal zinc ribbon / : / RNA polymerase I, Rpa2 specific domain / DNA-directed RNA polymerases I and III subunit AC19 / DNA-directed RNA polymerases I and III subunit AC40 / Zinc finger TFIIS-type signature. / RNA polymerase subunit Rpb7-like / RNA polymerase Rpb7-like , N-terminal / RNA polymerase Rpb7-like, N-terminal domain superfamily / SHS2 domain found in N terminus of Rpb7p/Rpc25p/MJ0397 / DNA-directed RNA polymerase, M/15kDa subunit / RNA polymerases M/15 Kd subunit / RNA polymerase subunit 9 / DNA-directed RNA polymerase subunit RPABC5/Rpb10 / RNA polymerases, subunit N, zinc binding site / RNA polymerase subunit RPB10 / RNA polymerases N / 8 kDa subunit / RNA polymerases N / 8 Kd subunits signature. / DNA-directed RNA polymerase M, 15kDa subunit, conserved site / RNA polymerases M / 15 Kd subunits signature. / DNA-directed RNA polymerase subunit/transcription factor S / RNA polymerase, Rpb8 / DNA-directed RNA polymerases I, II, and III subunit RPABC4 / RNA polymerase Rpb8 / RNA polymerase subunit 8 / RNA polymerase, Rpb5, N-terminal / RNA polymerase Rpb5, N-terminal domain superfamily / RNA polymerase Rpb5, N-terminal domain / DNA-directed RNA polymerase, subunit RPB6 / DNA-directed RNA polymerase subunit Rpo11 / DNA directed RNA polymerase, 7 kDa subunit / RNA polymerase archaeal subunit P/eukaryotic subunit RPABC4 / RNA polymerase, subunit H/Rpb5, conserved site / RNA polymerases H / 23 Kd subunits signature. / RNA polymerase subunit CX / DNA-directed RNA polymerase Rpb11, 13-16kDa subunit, conserved site / DNA-directed RNA polymerase subunit Rpo5/Rpb5 / RNA polymerases L / 13 to 16 Kd subunits signature. / Zinc finger, TFIIS-type / Transcription factor S-II (TFIIS) / Zinc finger TFIIS-type profile. / C2C2 Zinc finger / DNA-directed RNA polymerase, 30-40kDa subunit, conserved site / DNA-directed RNA polymerase subunit Rpo3/Rpb3/RPAC1 / RNA polymerases D / 30 to 40 Kd subunits signature. / DNA-directed RNA polymerase, RBP11-like dimerisation domain / RNA polymerase Rpb3/Rpb11 dimerisation domain / RNA polymerase, subunit H/Rpb5 C-terminal / RNA polymerase subunit RPABC4/transcription elongation factor Spt4 / RPB5-like RNA polymerase subunit superfamily / RNA polymerase Rpb5, C-terminal domain / Archaeal Rpo6/eukaryotic RPB6 RNA polymerase subunit / DNA-directed RNA polymerase, 14-18kDa subunit, conserved site / RNA polymerases K / 14 to 18 Kd subunits signature. / RNA polymerase Rpb6 / RNA polymerase, subunit omega/Rpo6/RPB6 / RNA polymerase Rpb6 / RNA polymerase Rpb1, domain 3 superfamily / RNA polymerase Rpb1, clamp domain superfamily / RPB6/omega subunit-like superfamily / DNA-directed RNA polymerase, subunit beta-prime / RNA polymerase Rpb1, domain 3 / RNA polymerase Rpb1, domain 3 / RNA polymerase Rpb2, domain 2 superfamily / RNA polymerase Rpb1, domain 1 / RNA polymerase Rpb1, domain 1 / RNA polymerase, alpha subunit / RNA polymerase Rpb1, domain 4 / RNA polymerase Rpb1, domain 2 / RNA polymerase Rpb1, domain 4 / RNA polymerase, N-terminal / RNA polymerase Rpb1, funnel domain superfamily / RNA polymerase I subunit A N-terminus / RNA polymerase Rpb1, domain 5 / RNA polymerase Rpb1, domain 5 / RNA polymerase, beta subunit, protrusion / RNA polymerase beta subunit / DNA-directed RNA polymerase, insert domain / DNA-directed RNA polymerase, RpoA/D/Rpb3-type / RNA polymerase Rpb3/RpoA insert domain / RNA polymerase Rpb3/Rpb11 dimerisation domain / RNA polymerases D / DNA-directed RNA polymerase, insert domain superfamily / RNA polymerase, RBP11-like subunit / RNA polymerase Rpb2, domain 2 / RNA polymerase Rpb2, domain 2 / RNA polymerase, beta subunit, conserved site / RNA polymerase Rpb2, domain 7 / RNA polymerase Rpb2, domain 3
Similarity search - Domain/homology
DNA-directed RNA polymerases I and III subunit RPAC1 / DNA-directed RNA polymerase I subunit RPA190 / DNA-directed RNA polymerases I, II, and III subunit RPABC1 / DNA-directed RNA polymerases I, II, and III subunit RPABC2 / DNA-directed RNA polymerases I, II, and III subunit RPABC3 / DNA-directed RNA polymerase I subunit RPA135 / DNA-directed RNA polymerases I, II, and III subunit RPABC5 / DNA-directed RNA polymerases I and III subunit RPAC2 / DNA-directed RNA polymerase I subunit RPA12 / DNA-directed RNA polymerases I, II, and III subunit RPABC4 ...DNA-directed RNA polymerases I and III subunit RPAC1 / DNA-directed RNA polymerase I subunit RPA190 / DNA-directed RNA polymerases I, II, and III subunit RPABC1 / DNA-directed RNA polymerases I, II, and III subunit RPABC2 / DNA-directed RNA polymerases I, II, and III subunit RPABC3 / DNA-directed RNA polymerase I subunit RPA135 / DNA-directed RNA polymerases I, II, and III subunit RPABC5 / DNA-directed RNA polymerases I and III subunit RPAC2 / DNA-directed RNA polymerase I subunit RPA12 / DNA-directed RNA polymerases I, II, and III subunit RPABC4 / DNA-directed RNA polymerase I subunit RPA43 / DNA-directed RNA polymerase I subunit RPA34 / DNA-directed RNA polymerase I subunit RPA14 / DNA-directed RNA polymerase I subunit RPA49
Similarity search - Component
Biological speciesSaccharomyces cerevisiae (brewer's yeast)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 5.5 Å
AuthorsKostrewa, D. / Kuhn, C.-D. / Engel, C. / Cramer, P.
Funding support Germany, 6items
OrganizationGrant numberCountry
Fonds der Chemischen Industrie Germany
Elite Network of Bavaria Germany
Boehringer Ingelheim Fonds Germany
Graduate Research Academy RNA Biology of SFB960 Germany
German Research Foundation Germany
Advanced Grant TRANSIT of the European Research Council Germany
CitationJournal: Acta Crystallogr.,Sect.D / Year: 2015
Title: An alternative RNA polymerase I structure reveals a dimer hinge.
Authors: Kostrewa, D. / Kuhn, C.D. / Engel, C. / Cramer, P.
History
DepositionMar 6, 2015Deposition site: RCSB / Processing site: PDBE
Revision 1.0Sep 9, 2015Provider: repository / Type: Initial release
Revision 1.1Sep 16, 2015Group: Database references
Revision 1.2Sep 6, 2017Group: Author supporting evidence / Category: pdbx_audit_support / Item: _pdbx_audit_support.funding_organization
Revision 1.3Jan 10, 2024Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
AA: DNA-directed RNA polymerase I subunit RPA190
AB: DNA-directed RNA polymerase I subunit RPA135
AC: DNA-directed RNA polymerases I and III subunit RPAC1
AD: DNA-directed RNA polymerase I subunit RPA14
AE: DNA-directed RNA polymerases I, II, and III subunit RPABC1
AF: DNA-directed RNA polymerases I, II, and III subunit RPABC2
AG: DNA-directed RNA polymerase I subunit RPA43
AH: DNA-directed RNA polymerases I, II, and III subunit RPABC3
AI: DNA-directed RNA polymerase I subunit RPA12
AJ: DNA-directed RNA polymerases I, II, and III subunit RPABC5
AK: DNA-directed RNA polymerases I and III subunit RPAC2
AL: DNA-directed RNA polymerases I, II, and III subunit RPABC4
AM: DNA-directed RNA polymerase I subunit RPA49
AN: DNA-directed RNA polymerase I subunit RPA34
AO: DNA-directed RNA polymerase I subunit RPA43
BA: DNA-directed RNA polymerase I subunit RPA190
BB: DNA-directed RNA polymerase I subunit RPA135
BC: DNA-directed RNA polymerases I and III subunit RPAC1
BD: DNA-directed RNA polymerase I subunit RPA14
BE: DNA-directed RNA polymerases I, II, and III subunit RPABC1
BF: DNA-directed RNA polymerases I, II, and III subunit RPABC2
BG: DNA-directed RNA polymerase I subunit RPA43
BH: DNA-directed RNA polymerases I, II, and III subunit RPABC3
BI: DNA-directed RNA polymerase I subunit RPA12
BJ: DNA-directed RNA polymerases I, II, and III subunit RPABC5
BK: DNA-directed RNA polymerases I and III subunit RPAC2
BL: DNA-directed RNA polymerases I, II, and III subunit RPABC4
BM: DNA-directed RNA polymerase I subunit RPA49
BN: DNA-directed RNA polymerase I subunit RPA34
BO: DNA-directed RNA polymerase I subunit RPA43
CA: DNA-directed RNA polymerase I subunit RPA190
CB: DNA-directed RNA polymerase I subunit RPA135
CC: DNA-directed RNA polymerases I and III subunit RPAC1
CD: DNA-directed RNA polymerase I subunit RPA14
CE: DNA-directed RNA polymerases I, II, and III subunit RPABC1
CF: DNA-directed RNA polymerases I, II, and III subunit RPABC2
CG: DNA-directed RNA polymerase I subunit RPA43
CH: DNA-directed RNA polymerases I, II, and III subunit RPABC3
CI: DNA-directed RNA polymerase I subunit RPA12
CJ: DNA-directed RNA polymerases I, II, and III subunit RPABC5
CK: DNA-directed RNA polymerases I and III subunit RPAC2
CL: DNA-directed RNA polymerases I, II, and III subunit RPABC4
CM: DNA-directed RNA polymerase I subunit RPA49
CN: DNA-directed RNA polymerase I subunit RPA34
CO: DNA-directed RNA polymerase I subunit RPA43
DA: DNA-directed RNA polymerase I subunit RPA190
DB: DNA-directed RNA polymerase I subunit RPA135
DC: DNA-directed RNA polymerases I and III subunit RPAC1
DD: DNA-directed RNA polymerase I subunit RPA14
DE: DNA-directed RNA polymerases I, II, and III subunit RPABC1
DF: DNA-directed RNA polymerases I, II, and III subunit RPABC2
DG: DNA-directed RNA polymerase I subunit RPA43
DH: DNA-directed RNA polymerases I, II, and III subunit RPABC3
DI: DNA-directed RNA polymerase I subunit RPA12
DJ: DNA-directed RNA polymerases I, II, and III subunit RPABC5
DK: DNA-directed RNA polymerases I and III subunit RPAC2
DL: DNA-directed RNA polymerases I, II, and III subunit RPABC4
DM: DNA-directed RNA polymerase I subunit RPA49
DN: DNA-directed RNA polymerase I subunit RPA34
DO: DNA-directed RNA polymerase I subunit RPA43
EA: DNA-directed RNA polymerase I subunit RPA190
EB: DNA-directed RNA polymerase I subunit RPA135
EC: DNA-directed RNA polymerases I and III subunit RPAC1
ED: DNA-directed RNA polymerase I subunit RPA14
EE: DNA-directed RNA polymerases I, II, and III subunit RPABC1
EF: DNA-directed RNA polymerases I, II, and III subunit RPABC2
EG: DNA-directed RNA polymerase I subunit RPA43
EH: DNA-directed RNA polymerases I, II, and III subunit RPABC3
EI: DNA-directed RNA polymerase I subunit RPA12
EJ: DNA-directed RNA polymerases I, II, and III subunit RPABC5
EK: DNA-directed RNA polymerases I and III subunit RPAC2
EL: DNA-directed RNA polymerases I, II, and III subunit RPABC4
EM: DNA-directed RNA polymerase I subunit RPA49
EN: DNA-directed RNA polymerase I subunit RPA34
EO: DNA-directed RNA polymerase I subunit RPA43
FA: DNA-directed RNA polymerase I subunit RPA190
FB: DNA-directed RNA polymerase I subunit RPA135
FC: DNA-directed RNA polymerases I and III subunit RPAC1
FD: DNA-directed RNA polymerase I subunit RPA14
FE: DNA-directed RNA polymerases I, II, and III subunit RPABC1
FF: DNA-directed RNA polymerases I, II, and III subunit RPABC2
FG: DNA-directed RNA polymerase I subunit RPA43
FH: DNA-directed RNA polymerases I, II, and III subunit RPABC3
FI: DNA-directed RNA polymerase I subunit RPA12
FJ: DNA-directed RNA polymerases I, II, and III subunit RPABC5
FK: DNA-directed RNA polymerases I and III subunit RPAC2
FL: DNA-directed RNA polymerases I, II, and III subunit RPABC4
FM: DNA-directed RNA polymerase I subunit RPA49
FN: DNA-directed RNA polymerase I subunit RPA34
FO: DNA-directed RNA polymerase I subunit RPA43
hetero molecules


Theoretical massNumber of molelcules
Total (without water)3,762,007132
Polymers3,759,26090
Non-polymers2,74742
Water0
1
AA: DNA-directed RNA polymerase I subunit RPA190
AB: DNA-directed RNA polymerase I subunit RPA135
AC: DNA-directed RNA polymerases I and III subunit RPAC1
AD: DNA-directed RNA polymerase I subunit RPA14
AE: DNA-directed RNA polymerases I, II, and III subunit RPABC1
AF: DNA-directed RNA polymerases I, II, and III subunit RPABC2
AG: DNA-directed RNA polymerase I subunit RPA43
AH: DNA-directed RNA polymerases I, II, and III subunit RPABC3
AI: DNA-directed RNA polymerase I subunit RPA12
AJ: DNA-directed RNA polymerases I, II, and III subunit RPABC5
AK: DNA-directed RNA polymerases I and III subunit RPAC2
AL: DNA-directed RNA polymerases I, II, and III subunit RPABC4
AM: DNA-directed RNA polymerase I subunit RPA49
AN: DNA-directed RNA polymerase I subunit RPA34
AO: DNA-directed RNA polymerase I subunit RPA43
hetero molecules


Theoretical massNumber of molelcules
Total (without water)627,00122
Polymers626,54315
Non-polymers4587
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
MethodPISA
2
BA: DNA-directed RNA polymerase I subunit RPA190
BB: DNA-directed RNA polymerase I subunit RPA135
BC: DNA-directed RNA polymerases I and III subunit RPAC1
BD: DNA-directed RNA polymerase I subunit RPA14
BE: DNA-directed RNA polymerases I, II, and III subunit RPABC1
BF: DNA-directed RNA polymerases I, II, and III subunit RPABC2
BG: DNA-directed RNA polymerase I subunit RPA43
BH: DNA-directed RNA polymerases I, II, and III subunit RPABC3
BI: DNA-directed RNA polymerase I subunit RPA12
BJ: DNA-directed RNA polymerases I, II, and III subunit RPABC5
BK: DNA-directed RNA polymerases I and III subunit RPAC2
BL: DNA-directed RNA polymerases I, II, and III subunit RPABC4
BM: DNA-directed RNA polymerase I subunit RPA49
BN: DNA-directed RNA polymerase I subunit RPA34
BO: DNA-directed RNA polymerase I subunit RPA43
hetero molecules


Theoretical massNumber of molelcules
Total (without water)627,00122
Polymers626,54315
Non-polymers4587
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
MethodPISA
3
CA: DNA-directed RNA polymerase I subunit RPA190
CB: DNA-directed RNA polymerase I subunit RPA135
CC: DNA-directed RNA polymerases I and III subunit RPAC1
CD: DNA-directed RNA polymerase I subunit RPA14
CE: DNA-directed RNA polymerases I, II, and III subunit RPABC1
CF: DNA-directed RNA polymerases I, II, and III subunit RPABC2
CG: DNA-directed RNA polymerase I subunit RPA43
CH: DNA-directed RNA polymerases I, II, and III subunit RPABC3
CI: DNA-directed RNA polymerase I subunit RPA12
CJ: DNA-directed RNA polymerases I, II, and III subunit RPABC5
CK: DNA-directed RNA polymerases I and III subunit RPAC2
CL: DNA-directed RNA polymerases I, II, and III subunit RPABC4
CM: DNA-directed RNA polymerase I subunit RPA49
CN: DNA-directed RNA polymerase I subunit RPA34
CO: DNA-directed RNA polymerase I subunit RPA43
hetero molecules


Theoretical massNumber of molelcules
Total (without water)627,00122
Polymers626,54315
Non-polymers4587
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
MethodPISA
4
DA: DNA-directed RNA polymerase I subunit RPA190
DB: DNA-directed RNA polymerase I subunit RPA135
DC: DNA-directed RNA polymerases I and III subunit RPAC1
DD: DNA-directed RNA polymerase I subunit RPA14
DE: DNA-directed RNA polymerases I, II, and III subunit RPABC1
DF: DNA-directed RNA polymerases I, II, and III subunit RPABC2
DG: DNA-directed RNA polymerase I subunit RPA43
DH: DNA-directed RNA polymerases I, II, and III subunit RPABC3
DI: DNA-directed RNA polymerase I subunit RPA12
DJ: DNA-directed RNA polymerases I, II, and III subunit RPABC5
DK: DNA-directed RNA polymerases I and III subunit RPAC2
DL: DNA-directed RNA polymerases I, II, and III subunit RPABC4
DM: DNA-directed RNA polymerase I subunit RPA49
DN: DNA-directed RNA polymerase I subunit RPA34
DO: DNA-directed RNA polymerase I subunit RPA43
hetero molecules


Theoretical massNumber of molelcules
Total (without water)627,00122
Polymers626,54315
Non-polymers4587
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
MethodPISA
5
EA: DNA-directed RNA polymerase I subunit RPA190
EB: DNA-directed RNA polymerase I subunit RPA135
EC: DNA-directed RNA polymerases I and III subunit RPAC1
ED: DNA-directed RNA polymerase I subunit RPA14
EE: DNA-directed RNA polymerases I, II, and III subunit RPABC1
EF: DNA-directed RNA polymerases I, II, and III subunit RPABC2
EG: DNA-directed RNA polymerase I subunit RPA43
EH: DNA-directed RNA polymerases I, II, and III subunit RPABC3
EI: DNA-directed RNA polymerase I subunit RPA12
EJ: DNA-directed RNA polymerases I, II, and III subunit RPABC5
EK: DNA-directed RNA polymerases I and III subunit RPAC2
EL: DNA-directed RNA polymerases I, II, and III subunit RPABC4
EM: DNA-directed RNA polymerase I subunit RPA49
EN: DNA-directed RNA polymerase I subunit RPA34
EO: DNA-directed RNA polymerase I subunit RPA43
hetero molecules


Theoretical massNumber of molelcules
Total (without water)627,00122
Polymers626,54315
Non-polymers4587
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
MethodPISA
6
FA: DNA-directed RNA polymerase I subunit RPA190
FB: DNA-directed RNA polymerase I subunit RPA135
FC: DNA-directed RNA polymerases I and III subunit RPAC1
FD: DNA-directed RNA polymerase I subunit RPA14
FE: DNA-directed RNA polymerases I, II, and III subunit RPABC1
FF: DNA-directed RNA polymerases I, II, and III subunit RPABC2
FG: DNA-directed RNA polymerase I subunit RPA43
FH: DNA-directed RNA polymerases I, II, and III subunit RPABC3
FI: DNA-directed RNA polymerase I subunit RPA12
FJ: DNA-directed RNA polymerases I, II, and III subunit RPABC5
FK: DNA-directed RNA polymerases I and III subunit RPAC2
FL: DNA-directed RNA polymerases I, II, and III subunit RPABC4
FM: DNA-directed RNA polymerase I subunit RPA49
FN: DNA-directed RNA polymerase I subunit RPA34
FO: DNA-directed RNA polymerase I subunit RPA43
hetero molecules


Theoretical massNumber of molelcules
Total (without water)627,00122
Polymers626,54315
Non-polymers4587
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
MethodPISA
Unit cell
Length a, b, c (Å)619.480, 306.620, 251.780
Angle α, β, γ (deg.)90.000, 97.500, 90.000
Int Tables number5
Space group name H-MC121
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
11chain AA
21chain BA
31chain CA
41chain DA
51chain EA
61chain FA
12chain AB
22chain BB
32chain CB
42chain DB
52chain EB
62chain FB
13chain AC
23chain BC
33chain CC
43chain DC
53chain EC
63chain FC
14chain AD
24chain BD
34chain CD
44chain DD
54chain ED
64chain FD
15chain AE
25chain BE
35chain CE
45chain DE
55chain EE
65chain FE
16chain AF
26chain BF
36chain CF
46chain DF
56chain EF
66chain FF
17chain AG
27chain BG
37chain CG
47chain DG
57chain EG
67chain FG
18chain AH
28chain BH
38chain CH
48chain DH
58chain EH
68chain FH
19chain AI
29chain BI
39chain CI
49chain DI
59chain EI
69chain FI
110chain AJ
210chain BJ
310chain CJ
410chain DJ
510chain EJ
610chain FJ
111chain AK
211chain BK
311chain CK
411chain DK
511chain EK
611chain FK
112chain AL
212chain BL
312chain CL
412chain DL
512chain EL
612chain FL
113chain AM
213chain BM
313chain CM
413chain DM
513chain EM
613chain FM
114chain AN
214chain BN
314chain CN
414chain DN
514chain EN
614chain FN

NCS domain segments:
Dom-IDComponent-IDEns-IDSelection detailsAuth asym-IDAuth seq-ID
111chain AAA0
211chain BAB0
311chain CAC0
411chain DAD0
511chain EAE0
611chain FAF0
112chain ABA0
212chain BBB0
312chain CBC0
412chain DBD0
512chain EBE0
612chain FBF0
113chain ACA0
213chain BCB0
313chain CCC0
413chain DCD0
513chain ECE0
613chain FCF0
114chain ADA0
214chain BDB0
314chain CDC0
414chain DDD0
514chain EDE0
614chain FDF0
115chain AEA0
215chain BEB0
315chain CEC0
415chain DED0
515chain EEE0
615chain FEF0
116chain AFA0
216chain BFB0
316chain CFC0
416chain DFD0
516chain EFE0
616chain FFF0
117chain AGA0
217chain BGB0
317chain CGC0
417chain DGD0
517chain EGE0
617chain FGF0
118chain AHA0
218chain BHB0
318chain CHC0
418chain DHD0
518chain EHE0
618chain FHF0
119chain AIA0
219chain BIB0
319chain CIC0
419chain DID0
519chain EIE0
619chain FIF0
1110chain AJA0
2110chain BJB0
3110chain CJC0
4110chain DJD0
5110chain EJE0
6110chain FJF0
1111chain AKA0
2111chain BKB0
3111chain CKC0
4111chain DKD0
5111chain EKE0
6111chain FKF0
1112chain ALA0
2112chain BLB0
3112chain CLC0
4112chain DLD0
5112chain ELE0
6112chain FLF0
1113chain AMA0
2113chain BMB0
3113chain CMC0
4113chain DMD0
5113chain EME0
6113chain FMF0
1114chain ANA0
2114chain BNB0
3114chain CNC0
4114chain DND0
5114chain ENE0
6114chain FNF0

NCS ensembles :
ID
1
2
3
4
5
6
7
8
9
10
11
12
13
14

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Components

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DNA-directed RNA polymerase I subunit ... , 7 types, 48 molecules AABACADAEAFAABBBCBDBEBFBADBDCDDDEDFDAGAOBGBOCGCODGDOEGEOFGFO...

#1: Protein
DNA-directed RNA polymerase I subunit RPA190 / Polymerase / DNA-directed RNA polymerase I 190 kDa polypeptide / A190 / DNA-directed RNA polymerase I largest subunit


Mass: 186676.969 Da / Num. of mol.: 6 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P10964, DNA-directed RNA polymerase
#2: Protein
DNA-directed RNA polymerase I subunit RPA135 / Polymerase / DNA-directed RNA polymerase I 135 kDa polypeptide / A135 / DNA-directed RNA polymerase I ...DNA-directed RNA polymerase I 135 kDa polypeptide / A135 / DNA-directed RNA polymerase I polypeptide 2 / RNA polymerase I subunit 2


Mass: 135910.328 Da / Num. of mol.: 6 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P22138, DNA-directed RNA polymerase
#4: Protein
DNA-directed RNA polymerase I subunit RPA14 / Polymerase / A14 / DNA-directed RNA polymerase I 14 kDa polypeptide


Mass: 14599.128 Da / Num. of mol.: 6 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P50106
#7: Protein
DNA-directed RNA polymerase I subunit RPA43 / Polymerase / A43 / DNA-directed DNA-dependent RNA polymerase 36 kDa polypeptide


Mass: 36264.852 Da / Num. of mol.: 12 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P46669
#9: Protein
DNA-directed RNA polymerase I subunit RPA12 / Polymerase / A12 / A12.2 / DNA-directed RNA polymerase I 13.7 kDa polypeptide


Mass: 13676.566 Da / Num. of mol.: 6 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P32529
#13: Protein
DNA-directed RNA polymerase I subunit RPA49 / Polymerase / A49 / DNA-directed RNA polymerase I 49 kDa polypeptide


Mass: 46721.707 Da / Num. of mol.: 6 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: Q01080
#14: Protein
DNA-directed RNA polymerase I subunit RPA34 / Polymerase / A34 / DNA-directed DNA-dependent RNA polymerase 34.5 kDa polypeptide / A34.5


Mass: 26933.518 Da / Num. of mol.: 6 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P47006

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DNA-directed RNA polymerases I and III subunit ... , 2 types, 12 molecules ACBCCCDCECFCAKBKCKDKEKFK

#3: Protein
DNA-directed RNA polymerases I and III subunit RPAC1 / RNA polymerases I and III subunit AC1 / C37 / DNA-directed RNA polymerases I and III 40 kDa polypeptide / C40


Mass: 37732.613 Da / Num. of mol.: 6 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P07703
#11: Protein
DNA-directed RNA polymerases I and III subunit RPAC2 / RNA polymerases I and III subunit AC2 / AC19 / DNA-directed RNA polymerases I and III 16 kDa ...RNA polymerases I and III subunit AC2 / AC19 / DNA-directed RNA polymerases I and III 16 kDa polypeptide / RPA19


Mass: 16167.860 Da / Num. of mol.: 6 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P28000

-
DNA-directed RNA polymerases I, II, and III subunit ... , 5 types, 30 molecules AEBECEDEEEFEAFBFCFDFEFFFAHBHCHDHEHFHAJBJCJDJEJFJALBLCLDLELFL

#5: Protein
DNA-directed RNA polymerases I, II, and III subunit RPABC1 / RNA polymerase / RNA polymerases I / II / and III subunit ABC1 / ABC27 / DNA-directed RNA polymerases I / and III 27 ...RNA polymerases I / II / and III subunit ABC1 / ABC27 / DNA-directed RNA polymerases I / and III 27 kDa polypeptide


Mass: 25117.094 Da / Num. of mol.: 6 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P20434
#6: Protein
DNA-directed RNA polymerases I, II, and III subunit RPABC2 / RNA polymerase / RNA polymerases I / II / and III subunit ABC2 / ABC23 / DNA-directed RNA polymerases I / and III 23 ...RNA polymerases I / II / and III subunit ABC2 / ABC23 / DNA-directed RNA polymerases I / and III 23 kDa polypeptide


Mass: 17931.834 Da / Num. of mol.: 6 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P20435
#8: Protein
DNA-directed RNA polymerases I, II, and III subunit RPABC3 / RNA polymerase / RNA polymerases I / II / and III subunit ABC3 / ABC14.4 / ABC14.5 / DNA-directed RNA polymerases I ...RNA polymerases I / II / and III subunit ABC3 / ABC14.4 / ABC14.5 / DNA-directed RNA polymerases I / and III 14.5 kDa polypeptide


Mass: 16525.363 Da / Num. of mol.: 6 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P20436
#10: Protein
DNA-directed RNA polymerases I, II, and III subunit RPABC5 / RNA polymerase / RNA polymerases I / II / and III subunit ABC5 / ABC10-beta / ABC8 / DNA-directed RNA polymerases I ...RNA polymerases I / II / and III subunit ABC5 / ABC10-beta / ABC8 / DNA-directed RNA polymerases I / and III 8.3 kDa polypeptide


Mass: 8290.732 Da / Num. of mol.: 6 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P22139
#12: Protein
DNA-directed RNA polymerases I, II, and III subunit RPABC4 / RNA polymerase / RNA polymerases I / II / and III subunit ABC4 / ABC10-alpha


Mass: 7729.969 Da / Num. of mol.: 6 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P40422

-
Non-polymers , 1 types, 42 molecules

#15: Chemical...
ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 42 / Source method: obtained synthetically / Formula: Zn

-
Experimental details

-
Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

-
Sample preparation

CrystalDensity Matthews: 3.15 Å3/Da / Density % sol: 60.99 %
Crystal growTemperature: 293 K / Method: vapor diffusion, sitting drop / pH: 7.5
Details: 10% PEG 6000, 3% MPD, 100 mM Hepes, 300 mM di-ammonium-tartrate, 3 mM TCEP

-
Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: SLS / Beamline: X06SA / Wavelength: 1.07392 Å
DetectorType: MARMOSAIC 225 mm CCD / Detector: CCD / Date: Nov 15, 2006
RadiationMonochromator: Si(111) silicon crystal / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1.07392 Å / Relative weight: 1
ReflectionResolution: 5.5→50 Å / Num. all: 149610 / Num. obs: 149610 / % possible obs: 98.9 % / Observed criterion σ(I): -3 / Redundancy: 6.1 % / Biso Wilson estimate: 171 Å2 / Rmerge(I) obs: 0.22 / Net I/σ(I): 6.6
Reflection shellResolution: 5.5→5.64 Å / Redundancy: 4.7 % / Rmerge(I) obs: 0.6 / Mean I/σ(I) obs: 2.5 / % possible all: 91

-
Processing

Software
NameClassification
XDSdata reduction
XSCALEdata scaling
PHASERphasing
PHENIXrefinement
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 4C2M

4c2m


Resolution: 5.5→49.685 Å / SU ML: 0.79 / Cross valid method: THROUGHOUT / Phase error: 24.15 / Stereochemistry target values: ML
RfactorNum. reflection% reflectionSelection details
Rfree0.2355 1986 1.33 %Random selection
Rwork0.1957 147617 --
obs0.1963 149603 99.07 %-
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso max: 338.48 Å2 / Biso mean: 195.9763 Å2 / Biso min: 141.7 Å2
Refinement stepCycle: final / Resolution: 5.5→49.685 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms204191 0 42 0 204233
Biso mean--204.71 --
Num. residues----25720
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.014208290
X-RAY DIFFRACTIONf_angle_d1.391281148
X-RAY DIFFRACTIONf_chiral_restr0.07431436
X-RAY DIFFRACTIONf_plane_restr0.00636379
X-RAY DIFFRACTIONf_dihedral_angle_d21.3478803
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDNumberRefine-IDTypeRms dev position (Å)
11AA11523X-RAY DIFFRACTIONPOSITIONAL0.071
12BA11523X-RAY DIFFRACTIONPOSITIONAL0.071
13CA11688X-RAY DIFFRACTIONPOSITIONAL0.072
14DA11679X-RAY DIFFRACTIONPOSITIONAL0.08
15EA11682X-RAY DIFFRACTIONPOSITIONAL0.077
16FA11682X-RAY DIFFRACTIONPOSITIONAL0.078
21AB9144X-RAY DIFFRACTIONPOSITIONAL0.072
22BB9144X-RAY DIFFRACTIONPOSITIONAL0.072
23CB9188X-RAY DIFFRACTIONPOSITIONAL0.072
24DB9179X-RAY DIFFRACTIONPOSITIONAL0.077
25EB9188X-RAY DIFFRACTIONPOSITIONAL0.078
26FB9188X-RAY DIFFRACTIONPOSITIONAL0.076
31AC2413X-RAY DIFFRACTIONPOSITIONAL0.065
32BC2413X-RAY DIFFRACTIONPOSITIONAL0.065
33CC2413X-RAY DIFFRACTIONPOSITIONAL0.072
34DC2413X-RAY DIFFRACTIONPOSITIONAL0.076
35EC2413X-RAY DIFFRACTIONPOSITIONAL0.078
36FC2413X-RAY DIFFRACTIONPOSITIONAL0.076
41AD459X-RAY DIFFRACTIONPOSITIONAL0.059
42BD459X-RAY DIFFRACTIONPOSITIONAL0.059
43CD459X-RAY DIFFRACTIONPOSITIONAL0.062
44DD459X-RAY DIFFRACTIONPOSITIONAL0.073
45ED459X-RAY DIFFRACTIONPOSITIONAL0.064
46FD459X-RAY DIFFRACTIONPOSITIONAL0.069
51AE1760X-RAY DIFFRACTIONPOSITIONAL0.069
52BE1760X-RAY DIFFRACTIONPOSITIONAL0.069
53CE1760X-RAY DIFFRACTIONPOSITIONAL0.074
54DE1760X-RAY DIFFRACTIONPOSITIONAL0.082
55EE1760X-RAY DIFFRACTIONPOSITIONAL0.077
56FE1760X-RAY DIFFRACTIONPOSITIONAL0.081
61AF807X-RAY DIFFRACTIONPOSITIONAL0.067
62BF807X-RAY DIFFRACTIONPOSITIONAL0.067
63CF807X-RAY DIFFRACTIONPOSITIONAL0.073
64DF807X-RAY DIFFRACTIONPOSITIONAL0.077
65EF807X-RAY DIFFRACTIONPOSITIONAL0.076
66FF807X-RAY DIFFRACTIONPOSITIONAL0.078
71AG1539X-RAY DIFFRACTIONPOSITIONAL0.064
72BG1539X-RAY DIFFRACTIONPOSITIONAL0.064
73CG1599X-RAY DIFFRACTIONPOSITIONAL0.069
74DG1599X-RAY DIFFRACTIONPOSITIONAL0.07
75EG1599X-RAY DIFFRACTIONPOSITIONAL0.072
76FG1599X-RAY DIFFRACTIONPOSITIONAL0.075
81AH1052X-RAY DIFFRACTIONPOSITIONAL0.074
82BH1052X-RAY DIFFRACTIONPOSITIONAL0.074
83CH1052X-RAY DIFFRACTIONPOSITIONAL0.073
84DH1063X-RAY DIFFRACTIONPOSITIONAL0.078
85EH1063X-RAY DIFFRACTIONPOSITIONAL0.078
86FH1063X-RAY DIFFRACTIONPOSITIONAL0.076
91AI718X-RAY DIFFRACTIONPOSITIONAL0.069
92BI718X-RAY DIFFRACTIONPOSITIONAL0.069
93CI945X-RAY DIFFRACTIONPOSITIONAL0.077
94DI945X-RAY DIFFRACTIONPOSITIONAL0.074
95EI900X-RAY DIFFRACTIONPOSITIONAL0.086
96FI945X-RAY DIFFRACTIONPOSITIONAL0.074
101AJ559X-RAY DIFFRACTIONPOSITIONAL0.067
102BJ559X-RAY DIFFRACTIONPOSITIONAL0.067
103CJ559X-RAY DIFFRACTIONPOSITIONAL0.076
104DJ559X-RAY DIFFRACTIONPOSITIONAL0.072
105EJ559X-RAY DIFFRACTIONPOSITIONAL0.081
106FJ559X-RAY DIFFRACTIONPOSITIONAL0.084
111AK786X-RAY DIFFRACTIONPOSITIONAL0.07
112BK786X-RAY DIFFRACTIONPOSITIONAL0.07
113CK793X-RAY DIFFRACTIONPOSITIONAL0.08
114DK793X-RAY DIFFRACTIONPOSITIONAL0.081
115EK786X-RAY DIFFRACTIONPOSITIONAL0.078
116FK786X-RAY DIFFRACTIONPOSITIONAL0.082
121AL353X-RAY DIFFRACTIONPOSITIONAL0.072
122BL353X-RAY DIFFRACTIONPOSITIONAL0.072
123CL353X-RAY DIFFRACTIONPOSITIONAL0.075
124DL353X-RAY DIFFRACTIONPOSITIONAL0.076
125EL353X-RAY DIFFRACTIONPOSITIONAL0.077
126FL353X-RAY DIFFRACTIONPOSITIONAL0.07
131AM863X-RAY DIFFRACTIONPOSITIONAL0.066
132BM863X-RAY DIFFRACTIONPOSITIONAL0.066
133CM863X-RAY DIFFRACTIONPOSITIONAL0.069
134DM863X-RAY DIFFRACTIONPOSITIONAL0.076
135EM863X-RAY DIFFRACTIONPOSITIONAL0.076
136FM863X-RAY DIFFRACTIONPOSITIONAL0.074
141AN1116X-RAY DIFFRACTIONPOSITIONAL0.074
142BN1116X-RAY DIFFRACTIONPOSITIONAL0.074
143CN1115X-RAY DIFFRACTIONPOSITIONAL0.074
144DN1116X-RAY DIFFRACTIONPOSITIONAL0.079
145EN1110X-RAY DIFFRACTIONPOSITIONAL0.081
146FN1116X-RAY DIFFRACTIONPOSITIONAL0.078
LS refinement shell

Refine-ID: X-RAY DIFFRACTION

Resolution (Å)Rfactor RfreeNum. reflection Rfree% reflection Rfree (%)Rfactor RworkNum. reflection Rwork% reflection obs (%)
5.5-5.63740.38661331.40.3132958091
5.6374-5.78960.31771471.40.29061051299
5.7896-5.95970.36591361.30.282310665100
5.9597-6.15170.30631381.30.272110550100
6.1517-6.37110.3311551.40.255910620100
6.3711-6.62570.28721471.40.233810631100
6.6257-6.92650.28161321.20.213510587100
6.9265-7.29060.22781481.40.188510567100
7.2906-7.74580.20781511.40.171610589100
7.7458-8.34120.1851531.40.140110604100
8.3412-9.17590.17831191.10.126210655100
9.1759-10.49280.15341651.50.116610659100
10.4928-13.17880.15591221.10.135210697100
13.1788-49.6870.23611401.30.241070199
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.28150.0154-0.21580.85840.21450.98760.04550.0584-0.1905-0.04590.1082-0.02410.4329-0.39720.00022.2182-0.1026-0.07752.42930.28752.492485.349285.3406-26.6758
21.047-0.1429-0.44990.79950.02420.92230.063-0.0991-0.43970.0396-0.0064-0.25520.43140.18880.00052.36020.2118-0.13432.1390.25432.6143124.101382.14147.851
30.7093-0.13110.36960.78880.64090.82740.0116-0.6857-0.54140.7811-0.5382-0.665-0.32770.4487-0.01352.8884-0.3637-0.26363.20740.462.8981120.02379.795956.7371
40.46690.25850.17330.516-0.06650.87650.00560.0704-0.00270.20740.16120.2165-0.0678-0.46580.00022.953-0.11850.28293.41750.20322.815952.883967.681790.3085
50.6033-0.1518-0.61240.9916-0.40720.91350.05480.0575-0.04130.1391-0.0887-0.26540.447-0.22360.00143.3102-0.4266-0.19723.18830.30662.865865.337225.01363.376
60.4883-0.32970.29190.5272-0.01950.2805-0.5418-0.3189-0.6053-0.5883-0.02540.02510.67830.6354-0.00684.4542-0.48980.4053.1406-0.19743.193875.343621.765415.5719
70.4569-0.2395-0.36210.4904-0.01090.83060.06980.0531-0.0495-0.0769-0.0079-0.08790.2575-0.04910.00011.9701-0.1253-0.13131.95120.07011.984887.3505174.223366.1176
81.34080.1684-0.45330.9671-0.55661.238-0.0317-0.0811-0.00210.0504-0.0506-0.143-0.00590.38160.00061.7096-0.1569-0.09211.7112-0.00951.9418112.2186206.57698.6558
91.4882-0.10230.02890.51540.21730.4074-0.4326-0.16930.14550.29790.2926-0.26210.04920.0193-0.00642.5183-0.24760.08581.97290.01852.1959109.3195207.196147.5302
100.35230.27930.14440.93230.23980.80970.06210.07210.09460.24010.13730.0669-0.0355-0.08150.00021.80840.06950.1222.05850.18082.008475.1269150.7016186.0185
111.32490.05430.57030.9186-0.5961.6257-0.14360.10850.03880.27070.1165-0.2318-0.24090.41730.00081.89980.19040.10941.8016-0.1421.9693117.2427131.4376162.7896
121.19940.13340.17050.50730.25850.4473-0.0784-0.1240.2002-0.2306-0.6573-0.5272-0.45610.786-0.01132.62870.34360.20212.48650.26892.247128.0456132.2919114.8582
131.02950.145-0.33650.2967-0.21760.92980.10250.18280.00920.01740.0407-0.03790.09810.020201.94120.0121-0.10561.7537-0.13351.900114.2033238.4705149.0214
141.19690.17590.54651.2395-0.61181.38560.0419-0.04560.3004-0.0385-0.1104-0.0338-0.06730.20670.00091.97850.07840.04721.8732-0.10861.87522.2482281.511176.3455
150.58980.3263-0.06120.4870.35950.48210.1129-0.4950.4234-0.087-0.43450.0767-0.54110.2305-0.01172.59230.37050.01522.69560.01972.3688-2.7554284.9624224.9059
160.3523-0.2977-0.09270.72340.26610.8139-0.0539-0.06740.0553-0.01140.15950.0807-0.1182-0.242-0.00021.7647-0.1305-0.03771.91310.13321.943319.1121228.3297271.2455
170.80640.23190.47010.94110.21731.5545-0.0535-0.10880.19070.08140.0297-0.1104-0.4003-0.08040.0011.8082-0.08620.12021.81640.06441.90161.3214247.3005247.6865
180.45790.413-0.2240.93070.2070.49890.02280.14310.4554-0.0782-0.3375-0.4192-0.60681.0389-0.00662.31880.13080.10472.63460.17662.211869.516250.9023199.4736
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain AA and (resid 654:1013 or resid 1262:1349 or resid 1435:1499) or chain AB and resid 13:1039 or chain AC or chain AH or chain AI or chain AJ or chain AK or chain AL or chain AM or chain ANA0
2X-RAY DIFFRACTION2chain AA and (resid 1:653 or resid 1014:1261 or resid 1500:1656 or resid 3001:3002) or chain AB and (resid 1040:1203 or resid 3001) or chain AE or chain AF and resid 73:154 or chain AOA0
3X-RAY DIFFRACTION3chain AA and resid 1657:1664 or chain AD or chain AF and resid 57:72 or chain AGA0
4X-RAY DIFFRACTION4chain BA and (resid 654:1013 or resid 1262:1348 or resid 1435:1499) or chain BB and resid 13:1039 or chain BC or chain BH or chain BI or chain BJ or chain BK or chain BL or chain BM or chain BNB0
5X-RAY DIFFRACTION5chain BA and (resid 1:653 or resid 1014:1261 or resid 1500:1656 or resid 3001:3002) or chain BB and (resid 1040:1203 or resid 3001) or chain BE or chain BF and resid 73:154 or chain BOB0
6X-RAY DIFFRACTION6chain BA and resid 1657:1664 or chain BD or chain BF and resid 57:72 or chain BGB0
7X-RAY DIFFRACTION7chain CA and (resid 654:1013 or resid 1262:1349 or resid 1435:1499) or chain CB and resid 9:1039 or chain CC or chain CH or chain CI or chain CJ or chain CK or chain CL or chain CM or chain CNC0
8X-RAY DIFFRACTION8chain CA and (resid 1:653 or resid 1014:1261 or resid 1500:1656 or resid 3001:3002) or chain CB and (resid 1040:1203 or resid 3001) or chain CE or chain CF and resid 73:154 or chain COC0
9X-RAY DIFFRACTION9chain CA and resid 1657:1664 or chain CD or chain CF and resid 56:72 or chain CGC0
10X-RAY DIFFRACTION10chain DA and (resid 654:1013 or resid 1262:1349 or resid 1434:1499) or chain DB and resid 8:1039 or chain DC or chain DH or chain DI or chain DJ or chain DK or chain DL or chain DM or chain DND0
11X-RAY DIFFRACTION11chain DA and (resid 1:653 or resid 1014:1261 or resid 1500:1656 or resid 3001:3002) or chain DB and (resid 1040:1203 or resid 3001) or chain DE or chain DF and resid 73:154 or chain DOD0
12X-RAY DIFFRACTION12chain DA and resid 1657:1664 or chain DD or chain DF and resid 56:72 or chain DGD0
13X-RAY DIFFRACTION13chain EA and (resid 654:1013 or resid 1262:1349 or resid 1435:1499) or chain EB and resid 12:1039 or chain EC or chain EH or chain EI or chain EJ or chain EK or chain EL or chain EM or chain ENE0
14X-RAY DIFFRACTION14chain EA and (resid 1:653 or resid 1014:1261 or resid 1500:1656 or resid 3001:3002) or chain EB and (resid 1040:1203 or resid 3001) or chain EE or chain EF and resid 73:154 or chain EOE0
15X-RAY DIFFRACTION15chain EA and resid 1657:1664 or chain ED or chain EF and resid 56:72 or chain EGE0
16X-RAY DIFFRACTION16chain FA and (resid 654:1013 or resid 1262:1349 or resid 1434:1499) or chain FB and resid 12:1039 or chain FC or chain FH or chain FI or chain FJ or chain FK or chain FL or chain FM or chain FNF0
17X-RAY DIFFRACTION17chain FA and (resid 1:653 or resid 1014:1261 or resid 1500:1656 or resid 3001:3002) or chain FB and (resid 1040:1203 or resid 3001) or chain FE or chain FF and resid 73:154 or chain FOF0
18X-RAY DIFFRACTION18chain FA and resid 1657:1664 or chain FD or chain FF and resid 56:72 or chain FGF0

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