4YM7

RNA polymerase I structure with an alternative dimer hinge

This is a non-PDB format compatible entry.

Summary for 4YM7

• Entry DOI: 10.2210/pdb4ym7/pdb
• Descriptor: DNA-directed RNA polymerase I subunit RPA190, DNA-directed RNA polymerases I, II, and III subunit RPABC5, DNA-directed RNA polymerases I and III subunit RPAC2, ... (15 entities in total)
• Functional Keywords: rna polymerase i, dimer hinge, transferase
• Biological source: Saccharomyces cerevisiae (Baker's yeast); More
• Cellular location: Nucleus, nucleolus : P10964 P22139 P28000 P40422 Q01080 P47006 P22138 P07703 P50106 P46669 P32529; Nucleus: P20434 P20436; Cytoplasm : P20435
• Total number of polymer chains: 90
• Total formula weight: 3762007.49

Authors

Kostrewa, D.,Kuhn, C.-D.,Engel, C.,Cramer, P. (deposition date: 2015-03-06, release date: 2015-09-09, Last modification date: 2024-11-20)

Primary citation

Kostrewa, D.,Kuhn, C.D.,Engel, C.,Cramer, P.
An alternative RNA polymerase I structure reveals a dimer hinge.
Acta Crystallogr.,Sect.D, 71:1850-1855, 2015

PubMed Abstract: RNA polymerase I (Pol I) is the central, 14-subunit enzyme that synthesizes the ribosomal RNA (rRNA) precursor in eukaryotic cells. The recent crystal structure of Pol I at 2.8 Å resolution revealed two novel elements: the `expander' in the active-centre cleft and the `connector' that mediates Pol I dimerization [Engel et al. (2013), Nature (London), 502, 650-655]. Here, a Pol I structure in an alternative crystal form that was solved by molecular replacement using the original atomic Pol I structure is reported. The resulting alternative structure lacks the expander but still shows an expanded active-centre cleft. The neighbouring Pol I monomers form a homodimer with a relative orientation distinct from that observed previously, establishing the connector as a hinge between Pol I monomers.

PubMed: 26327374
DOI: 10.1107/S1399004715012651

Experimental method

X-RAY DIFFRACTION (5.5 Å)