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- PDB-4c2m: Structure of RNA polymerase I at 2.8 A resolution -

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Basic information

Entry
Database: PDB / ID: 4c2m
TitleStructure of RNA polymerase I at 2.8 A resolution
Components
  • (DNA-DIRECTED RNA POLYMERASE I SUBUNIT ...Polymerase) x 7
  • (DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT ...) x 2
  • (DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC ...RNA polymerase) x 5
KeywordsTRANSCRIPTION / RIBOSOME BIOGENESIS
Function / homology
Function and homology information


RNA polymerase III activity / RNA polymerase I preinitiation complex assembly / RNA Polymerase I Transcription Initiation / Processing of Capped Intron-Containing Pre-mRNA / : / RNA Polymerase III Transcription Initiation From Type 2 Promoter / RNA Pol II CTD phosphorylation and interaction with CE / regulation of cell size / Formation of the Early Elongation Complex / mRNA Capping ...RNA polymerase III activity / RNA polymerase I preinitiation complex assembly / RNA Polymerase I Transcription Initiation / Processing of Capped Intron-Containing Pre-mRNA / : / RNA Polymerase III Transcription Initiation From Type 2 Promoter / RNA Pol II CTD phosphorylation and interaction with CE / regulation of cell size / Formation of the Early Elongation Complex / mRNA Capping / Formation of TC-NER Pre-Incision Complex / RNA polymerase II transcribes snRNA genes / RNA Polymerase I Promoter Escape / TP53 Regulates Transcription of DNA Repair Genes / Estrogen-dependent gene expression / RNA Polymerase II Promoter Escape / RNA Polymerase II Transcription Pre-Initiation And Promoter Opening / RNA Polymerase II Transcription Initiation / RNA Polymerase II Transcription Initiation And Promoter Clearance / RNA-templated transcription / termination of RNA polymerase III transcription / RNA Polymerase II Pre-transcription Events / Dual incision in TC-NER / RNA polymerase I activity / termination of RNA polymerase I transcription / transcription initiation at RNA polymerase III promoter / tRNA transcription by RNA polymerase III / nucleolar large rRNA transcription by RNA polymerase I / Gap-filling DNA repair synthesis and ligation in TC-NER / transcription initiation at RNA polymerase I promoter / transcription elongation by RNA polymerase I / RNA polymerase I complex / transcription by RNA polymerase I / RNA polymerase III complex / transcription by RNA polymerase III / RNA polymerase II, core complex / promoter-specific chromatin binding / transcription elongation by RNA polymerase II / transcription initiation at RNA polymerase II promoter / ribonucleoside binding / DNA-directed RNA polymerase / peroxisome / ribosome biogenesis / RNA polymerase II-specific DNA-binding transcription factor binding / transcription by RNA polymerase II / nucleic acid binding / protein dimerization activity / nucleolus / negative regulation of transcription by RNA polymerase II / DNA binding / zinc ion binding / nucleoplasm / metal ion binding / nucleus / cytoplasm
Similarity search - Function
Alpha-Beta Plaits - #2850 / Helix Hairpins - #1770 / Tetracycline Repressor; domain 2 - #120 / Single alpha-helices involved in coiled-coils or other helix-helix interfaces - #3390 / RNA polymerase ii / RNA polymerase Rpb1, domain 3 / RNA polymerase Rpb1 funnel domain / RNA polymerase I, subunit Rpa14, fungi / Yeast RNA polymerase I subunit RPA14 / DNA-directed RNA polymerase I, subunit RPA34.5 ...Alpha-Beta Plaits - #2850 / Helix Hairpins - #1770 / Tetracycline Repressor; domain 2 - #120 / Single alpha-helices involved in coiled-coils or other helix-helix interfaces - #3390 / RNA polymerase ii / RNA polymerase Rpb1, domain 3 / RNA polymerase Rpb1 funnel domain / RNA polymerase I, subunit Rpa14, fungi / Yeast RNA polymerase I subunit RPA14 / DNA-directed RNA polymerase I, subunit RPA34.5 / DNA-directed RNA polymerase I subunit RPA34.5 / RNA polymerase I associated factor, A49-like / A49-like RNA polymerase I associated factor / Rpa43, N-terminal ribonucleoprotein (RNP) domain / RPA43, OB domain / RPA43 OB domain in RNA Pol I / DNA-directed RNA polymerase I subunit RPA2, domain 4 / DNA-directed RNA pol I, largest subunit / Pol I subunit A12, C-terminal zinc ribbon / : / RNA polymerase I, Rpa2 specific domain / Hypothetical Protein Ta0175; Chain: A, domain 2 - #20 / DCoH-like / RNA polymerase alpha subunit dimerisation domain / Enzyme I; Chain A, domain 2 / Hypothetical Protein Ta0175; Chain: A, domain 2 / Dna-directed Rna Polymerases I, Ii, And Iii 27 Kd Polypeptide; Chain: A; domain 1 / RNA polymerase, Rpb5, N-terminal domain / RNA polymerase ii, chain L / RPB5-like RNA polymerase subunit / Topoisomerase I; Chain A, domain 4 / DNA-directed RNA polymerases I and III subunit AC19 / DNA-directed RNA polymerases I and III subunit AC40 / Dna-directed Rna Polymerase Ii 140kd Polypeptide; Chain: B; domain 3 / RNA polymerase Rpb2, domain 2 / Dna-directed Rna Polymerase Ii 140kd Polypeptide; Chain: B; Domain 6 / DNA-directed RNA polymerase, subunit 2, domain 6 / RNA polymerase II, Rpb2 subunit, wall domain / Barwin-like endoglucanases - #20 / Barwin-like endoglucanases / RNA polymerase subunit, RPB6/omega / Eukaryotic RPB6 RNA polymerase subunit / DNA-directed RNA polymerase, insert domain / RNA polymerase, RBP11-like subunit / RNA Polymerase Alpha Subunit; Chain A, domain 2 / Rubrerythrin, domain 2 / Tetracycline Repressor; domain 2 / Helix Hairpins / Gyrase A; domain 2 / Zinc finger TFIIS-type signature. / Single alpha-helices involved in coiled-coils or other helix-helix interfaces / RNA polymerase subunit Rpb7-like / RNA polymerase Rpb7-like , N-terminal / RNA polymerase Rpb7-like, N-terminal domain superfamily / SHS2 domain found in N terminus of Rpb7p/Rpc25p/MJ0397 / DNA-directed RNA polymerase subunit RPABC5/Rpb10 / RNA polymerases, subunit N, zinc binding site / RNA polymerase subunit RPB10 / RNA polymerases N / 8 kDa subunit / RNA polymerases N / 8 Kd subunits signature. / DNA-directed RNA polymerase, M/15kDa subunit / RNA polymerases M/15 Kd subunit / RNA polymerase subunit 9 / DNA-directed RNA polymerase M, 15kDa subunit, conserved site / RNA polymerases M / 15 Kd subunits signature. / DNA-directed RNA polymerase subunit/transcription factor S / RNA polymerase, Rpb8 / DNA-directed RNA polymerases I, II, and III subunit RPABC4 / RNA polymerase Rpb8 / RNA polymerase subunit 8 / RNA polymerase, Rpb5, N-terminal / RNA polymerase Rpb5, N-terminal domain superfamily / RNA polymerase Rpb5, N-terminal domain / DNA-directed RNA polymerase, subunit RPB6 / DNA-directed RNA polymerase subunit Rpo11 / DNA directed RNA polymerase, 7 kDa subunit / RNA polymerase archaeal subunit P/eukaryotic subunit RPABC4 / RNA polymerase, subunit H/Rpb5, conserved site / RNA polymerases H / 23 Kd subunits signature. / RNA polymerase subunit CX / DNA-directed RNA polymerase Rpb11, 13-16kDa subunit, conserved site / DNA-directed RNA polymerase subunit Rpo5/Rpb5 / RNA polymerases L / 13 to 16 Kd subunits signature. / DNA-directed RNA polymerase, 30-40kDa subunit, conserved site / DNA-directed RNA polymerase subunit Rpo3/Rpb3/RPAC1 / RNA polymerases D / 30 to 40 Kd subunits signature. / DNA-directed RNA polymerase, RBP11-like dimerisation domain / RNA polymerase Rpb3/Rpb11 dimerisation domain / RNA polymerase, subunit H/Rpb5 C-terminal / RNA polymerase subunit RPABC4/transcription elongation factor Spt4 / RPB5-like RNA polymerase subunit superfamily / RNA polymerase Rpb5, C-terminal domain / Zinc finger, TFIIS-type / Transcription factor S-II (TFIIS) / Zinc finger TFIIS-type profile. / C2C2 Zinc finger / Archaeal Rpo6/eukaryotic RPB6 RNA polymerase subunit / DNA-directed RNA polymerase, 14-18kDa subunit, conserved site / RNA polymerases K / 14 to 18 Kd subunits signature. / Homeodomain-like
Similarity search - Domain/homology
DNA-directed RNA polymerases I and III subunit RPAC1 / DNA-directed RNA polymerase I subunit RPA190 / DNA-directed RNA polymerases I, II, and III subunit RPABC1 / DNA-directed RNA polymerases I, II, and III subunit RPABC2 / DNA-directed RNA polymerases I, II, and III subunit RPABC3 / DNA-directed RNA polymerase I subunit RPA135 / DNA-directed RNA polymerases I, II, and III subunit RPABC5 / DNA-directed RNA polymerases I and III subunit RPAC2 / DNA-directed RNA polymerase I subunit RPA12 / DNA-directed RNA polymerases I, II, and III subunit RPABC4 ...DNA-directed RNA polymerases I and III subunit RPAC1 / DNA-directed RNA polymerase I subunit RPA190 / DNA-directed RNA polymerases I, II, and III subunit RPABC1 / DNA-directed RNA polymerases I, II, and III subunit RPABC2 / DNA-directed RNA polymerases I, II, and III subunit RPABC3 / DNA-directed RNA polymerase I subunit RPA135 / DNA-directed RNA polymerases I, II, and III subunit RPABC5 / DNA-directed RNA polymerases I and III subunit RPAC2 / DNA-directed RNA polymerase I subunit RPA12 / DNA-directed RNA polymerases I, II, and III subunit RPABC4 / DNA-directed RNA polymerase I subunit RPA43 / DNA-directed RNA polymerase I subunit RPA34 / DNA-directed RNA polymerase I subunit RPA14 / DNA-directed RNA polymerase I subunit RPA49
Similarity search - Component
Biological speciesSACCHAROMYCES CEREVISIAE (brewer's yeast)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MAD / Resolution: 2.8 Å
AuthorsEngel, C. / Sainsbury, S. / Cheung, A.C. / Kostrewa, D. / Cramer, P.
CitationJournal: Nature / Year: 2013
Title: RNA Polymerase I Structure and Transcription Regulation.
Authors: Engel, C. / Sainsbury, S. / Cheung, A.C. / Kostrewa, D. / Cramer, P.
History
DepositionAug 19, 2013Deposition site: PDBE / Processing site: PDBE
Revision 1.0Oct 23, 2013Provider: repository / Type: Initial release
Revision 1.1Nov 6, 2013Group: Database references
Revision 1.2Aug 19, 2015Group: Derived calculations
Remark 700 SHEET DETERMINATION METHOD: DSSP THE SHEETS PRESENTED AS "2B" IN EACH CHAIN ON SHEET RECORDS BELOW ... SHEET DETERMINATION METHOD: DSSP THE SHEETS PRESENTED AS "2B" IN EACH CHAIN ON SHEET RECORDS BELOW IS ACTUALLY AN 7-STRANDED BARREL THIS IS REPRESENTED BY A 8-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS ARE IDENTICAL. THE SHEETS PRESENTED AS "AF" IN EACH CHAIN ON SHEET RECORDS BELOW IS ACTUALLY AN 7-STRANDED BARREL THIS IS REPRESENTED BY A 8-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS ARE IDENTICAL. THE SHEETS PRESENTED AS "AI" IN EACH CHAIN ON SHEET RECORDS BELOW IS ACTUALLY AN 9-STRANDED BARREL THIS IS REPRESENTED BY A 10-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS ARE IDENTICAL. THE SHEETS PRESENTED AS "GC" IN EACH CHAIN ON SHEET RECORDS BELOW IS ACTUALLY AN 5-STRANDED BARREL THIS IS REPRESENTED BY A 6-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS ARE IDENTICAL. THE SHEETS PRESENTED AS "MB" IN EACH CHAIN ON SHEET RECORDS BELOW IS ACTUALLY AN 8-STRANDED BARREL THIS IS REPRESENTED BY A 9-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS ARE IDENTICAL. THE SHEETS PRESENTED AS "PF" IN EACH CHAIN ON SHEET RECORDS BELOW IS ACTUALLY AN 7-STRANDED BARREL THIS IS REPRESENTED BY A 8-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS ARE IDENTICAL. THE SHEETS PRESENTED AS "PI" IN EACH CHAIN ON SHEET RECORDS BELOW IS ACTUALLY AN 9-STRANDED BARREL THIS IS REPRESENTED BY A 10-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS ARE IDENTICAL. THE SHEETS PRESENTED AS "VC" IN EACH CHAIN ON SHEET RECORDS BELOW IS ACTUALLY AN 5-STRANDED BARREL THIS IS REPRESENTED BY A 6-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS ARE IDENTICAL.

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
1: DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 4
2: DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA49
3: DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA34
4: DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA43
A: DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA190
B: DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA135
C: DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT RPAC1
D: DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA14
E: DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 1
F: DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 2
G: DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA43
H: DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 3
I: DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA12
J: DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 5
K: DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT RPAC2
L: DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 4
M: DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA49
N: DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA34
O: DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA43
P: DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA190
Q: DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA135
R: DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT RPAC1
S: DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA14
T: DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 1
U: DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 2
V: DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA43
W: DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 3
X: DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA12
Y: DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 5
Z: DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT RPAC2
hetero molecules


Theoretical massNumber of molelcules
Total (without water)1,254,19546
Polymers1,253,08730
Non-polymers1,10816
Water4,720262
1
A: DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA190
B: DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA135
C: DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT RPAC1
D: DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA14
E: DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 1
F: DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 2
G: DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA43
H: DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 3
I: DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA12
J: DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 5
K: DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT RPAC2
L: DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 4
M: DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA49
N: DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA34
O: DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA43
hetero molecules


Theoretical massNumber of molelcules
Total (without water)627,09723
Polymers626,54315
Non-polymers5548
Water19811
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area99110 Å2
ΔGint-545.1 kcal/mol
Surface area204050 Å2
MethodPQS
2
1: DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 4
2: DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA49
3: DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA34
4: DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA43
P: DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA190
Q: DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA135
R: DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT RPAC1
S: DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA14
T: DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 1
U: DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 2
V: DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA43
W: DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 3
X: DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA12
Y: DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 5
Z: DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT RPAC2
hetero molecules


Theoretical massNumber of molelcules
Total (without water)627,09723
Polymers626,54315
Non-polymers5548
Water18010
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area98310 Å2
ΔGint-543.3 kcal/mol
Surface area203550 Å2
MethodPQS
Unit cell
Length a, b, c (Å)122.740, 139.020, 209.550
Angle α, β, γ (deg.)108.06, 95.40, 93.85
Int Tables number1
Space group name H-MP1
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-ID
11
21
12
22
13
23
14
24
15
25
16
26
17
27
18
28
19
29
110
210
111
211
112
212
113
213
114
214
115
215
116
216
117
217
118
218
119
219
120
220
121
221
122
222
123
223
124
224
125
225
126
226
127
227
128
228
129
229
130
230
131
231
132
232
133
233
134
234
135
235
136
236
137
237
138
238
139
239
140
240
141
241
142
242
143
243
144
244

NCS domain segments:
Dom-IDComponent-IDEns-IDSelection details
111CHAIN A AND RESSEQ 1:84
211CHAIN P AND RESSEQ 1:84
112CHAIN A AND RESSEQ 85:139
212CHAIN P AND RESSEQ 85:139
113CHAIN A AND RESSEQ 176:272
213CHAIN P AND RESSEQ 176:272
114CHAIN A AND RESSEQ 296:363
214CHAIN P AND RESSEQ 296:363
115CHAIN A AND RESSEQ 364:380
215CHAIN P AND RESSEQ 364:380
116CHAIN A AND RESSEQ 381:441
216CHAIN P AND RESSEQ 381:441
117CHAIN A AND RESSEQ 442:477
217CHAIN P AND RESSEQ 442:477
118CHAIN A AND RESSEQ 478:652
218CHAIN P AND RESSEQ 478:652
119CHAIN A AND RESSEQ 653:826
219CHAIN P AND RESSEQ 653:826
1110CHAIN A AND RESSEQ 827:989
2110CHAIN P AND RESSEQ 827:989
1111CHAIN A AND RESSEQ 990:1028
2111CHAIN P AND RESSEQ 990:1028
1112CHAIN A AND RESSEQ 1029:1152
2112CHAIN P AND RESSEQ 1029:1152
1113CHAIN A AND RESSEQ 1160:1204
2113CHAIN P AND RESSEQ 1160:1204
1114CHAIN A AND RESSEQ 1213:1337
2114CHAIN P AND RESSEQ 1213:1337
1115CHAIN A AND RESSEQ 1361:1395
2115CHAIN P AND RESSEQ 1361:1395
1116CHAIN A AND RESSEQ 1596:1664
2116CHAIN P AND RESSEQ 1596:1664
1117CHAIN B AND RESSEQ 8:79
2117CHAIN Q AND RESSEQ 8:79
1118CHAIN B AND RESSEQ 88:204
2118CHAIN Q AND RESSEQ 88:204
1119CHAIN B AND RESSEQ 205:404
2119CHAIN Q AND RESSEQ 205:404
1120CHAIN B AND RESSEQ 405:489
2120CHAIN Q AND RESSEQ 405:489
1121CHAIN B AND RESSEQ 490:697
2121CHAIN Q AND RESSEQ 490:697
1122CHAIN B AND RESSEQ 698:798
2122CHAIN Q AND RESSEQ 698:798
1123CHAIN B AND RESSEQ 799:910
2123CHAIN Q AND RESSEQ 799:910
1124CHAIN B AND RESSEQ 911:1038
2124CHAIN Q AND RESSEQ 911:1038
1125CHAIN B AND RESSEQ 1039:1203
2125CHAIN Q AND RESSEQ 1039:1203
1126CHAIN C AND RESSEQ 31:76
2126CHAIN R AND RESSEQ 31:76
1127CHAIN C AND RESSEQ 77:220
2127CHAIN R AND RESSEQ 77:220
1128CHAIN C AND RESSEQ 221:334
2128CHAIN R AND RESSEQ 221:334
1129CHAIN D
2129CHAIN S
1130CHAIN G AND RESSEQ 14:127
2130CHAIN V AND RESSEQ 14:127
1131CHAIN G AND RESSEQ 128:250
2131CHAIN V AND RESSEQ 128:250
1132CHAIN O AND RESSEQ 265:315
2132CHAIN 4 AND RESSEQ 265:315
1133CHAIN E AND RESSEQ 5:147
2133CHAIN T AND RESSEQ 5:147
1134CHAIN E AND RESSEQ 148:215
2134CHAIN T AND RESSEQ 148:215
1135CHAIN F
2135CHAIN U
1136CHAIN H
2136CHAIN W
1137CHAIN I AND RESSEQ 2:74
2137CHAIN X AND RESSEQ 2:74
1138CHAIN I AND RESSEQ 75:125
2138CHAIN X AND RESSEQ 75:125
1139CHAIN J
2139CHAIN Y
1140CHAIN K
2140CHAIN Z
1141CHAIN L
2141CHAIN 1
1142CHAIN M
2142CHAIN 2
1143CHAIN N AND RESSEQ 22:140
2143CHAIN 3 AND RESSEQ 22:140
1144CHAIN N AND RESSEQ 141:180
2144CHAIN 3 AND RESSEQ 141:180

NCS ensembles :
ID
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44

NCS oper: (Code: given
Matrix: (-0.9923, -0.1217, -0.02281), (-0.1221, 0.9923, 0.02118), (0.02006, 0.0238, -0.9995)
Vector: 106.6, 7.927, 212.8)

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Components

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DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC ... , 5 types, 10 molecules 1LETFUHWJY

#1: Protein DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 4 / RNA polymerase / RNA POLYMERASES I\ / II\ / AND III SUBUNIT ABC4 / ABC10-ALPHA / RPB12


Mass: 7729.969 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) SACCHAROMYCES CEREVISIAE (brewer's yeast) / Strain: CB010 / References: UniProt: P40422
#9: Protein DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 1 / RNA polymerase / RNA POLYMERASES I / II / AND III SUBUNIT ABC1 / ABC27 / DNA-DIRECTED RNA POLYMERASES I\ / II\ / AND ...RNA POLYMERASES I / II / AND III SUBUNIT ABC1 / ABC27 / DNA-DIRECTED RNA POLYMERASES I\ / II\ / AND III 27 KDA POLYPEPTIDE / RPB5


Mass: 25117.094 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) SACCHAROMYCES CEREVISIAE (brewer's yeast) / Strain: CB010 / References: UniProt: P20434
#10: Protein DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 2 / RNA polymerase / RNA POLYMERASES I\ / II\ / AND III SUBUNIT ABC2 / ABC23 / DNA-DIRECTED RNA POLYMERASES I\ / II\ / ...RNA POLYMERASES I\ / II\ / AND III SUBUNIT ABC2 / ABC23 / DNA-DIRECTED RNA POLYMERASES I\ / II\ / AND III 23 KDA POLYPEPTIDE / RPB6


Mass: 17931.834 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) SACCHAROMYCES CEREVISIAE (brewer's yeast) / Strain: CB010 / References: UniProt: P20435
#11: Protein DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 3 / RNA polymerase / RNA POLYMERASES I\ / II\ / AND III SUBUNIT ABC3 / ABC14.4 / ABC14.5 / DNA-DIRECTED RNA POLYMERASES ...RNA POLYMERASES I\ / II\ / AND III SUBUNIT ABC3 / ABC14.4 / ABC14.5 / DNA-DIRECTED RNA POLYMERASES I\ / II\ / AND III 14.5 KDA POLYPEPTIDE / RPB8


Mass: 16525.363 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) SACCHAROMYCES CEREVISIAE (brewer's yeast) / Strain: CB010 / References: UniProt: P20436
#13: Protein DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 5 / RNA polymerase / RNA POLYMERASES I\ / II\ / AND III SUBUNIT ABC5 / ABC10-BETA / ABC8 / DNA-DIRECTED RNA POLYMERASES ...RNA POLYMERASES I\ / II\ / AND III SUBUNIT ABC5 / ABC10-BETA / ABC8 / DNA-DIRECTED RNA POLYMERASES I\ / II\ / AND III 8.3 KDA POLYPEPTIDE / RPB10


Mass: 8290.732 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) SACCHAROMYCES CEREVISIAE (brewer's yeast) / Strain: CB010 / References: UniProt: P22139

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DNA-DIRECTED RNA POLYMERASE I SUBUNIT ... , 7 types, 16 molecules 2M3N4GOVAPBQDSIX

#2: Protein DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA49 / Polymerase / A49 / DNA-DIRECTED RNA POLYMERASE I 49 KDA POLYPEPTIDE


Mass: 46721.707 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) SACCHAROMYCES CEREVISIAE (brewer's yeast) / Strain: CB010 / References: UniProt: Q01080
#3: Protein DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA34 / Polymerase / A34 / DNA-DIRECTED DNA-DEPENDENT RNA POLYMERASE 34.5 KDA POLYPEPTIDE / A34.5 / A34.5


Mass: 26933.518 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) SACCHAROMYCES CEREVISIAE (brewer's yeast) / Strain: CB010 / References: UniProt: P47006
#4: Protein
DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA43 / Polymerase / A43 / DNA-DIRECTED DNA-DEPENDENT RNA POLYMERASE 36 KDA POLYPEPTIDE / A43


Mass: 36264.852 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) SACCHAROMYCES CEREVISIAE (brewer's yeast) / Strain: CB010 / References: UniProt: P46669
#5: Protein DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA190 / Polymerase / A190 / DNA-DIRECTED RNA POLYMERASE I 190 KDA POLYPEPTIDE / DNA-DIRECTED RNA POLYMERASE I LARGEST SUBUNIT


Mass: 186676.969 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) SACCHAROMYCES CEREVISIAE (brewer's yeast) / Strain: CB010 / References: UniProt: P10964, DNA-directed RNA polymerase
#6: Protein DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA135 / Polymerase / DNA-DIRECTED RNA POLYMERASE I 135 KDA POLYPEPTIDE / A135 / DNA-DIRECTED RNA POLYMERASE I ...DNA-DIRECTED RNA POLYMERASE I 135 KDA POLYPEPTIDE / A135 / DNA-DIRECTED RNA POLYMERASE I POLYPEPTIDE 2 / RNA POLYMERASE I SUBUNIT 2 / A135


Mass: 135910.328 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) SACCHAROMYCES CEREVISIAE (brewer's yeast) / Strain: CB010 / References: UniProt: P22138, DNA-directed RNA polymerase
#8: Protein DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA14 / Polymerase / A14 / DNA-DIRECTED RNA POLYMERASE I 14 KDA POLYPEPTIDE / A14


Mass: 14599.128 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) SACCHAROMYCES CEREVISIAE (brewer's yeast) / Strain: CB010 / References: UniProt: P50106
#12: Protein DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA12 / Polymerase / A12 / A12.2 / DNA-DIRECTED RNA POLYMERASE I 13.7 KDA POLYPEPTIDE / A12.2


Mass: 13676.566 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) SACCHAROMYCES CEREVISIAE (brewer's yeast) / Strain: CB010 / References: UniProt: P32529

-
DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT ... , 2 types, 4 molecules CRKZ

#7: Protein DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT RPAC1 / RNA POLYMERASES I AND III SUBUNIT AC1 / C37 / DNA-DIRECTED RN A POLYMERASES I AND III 40 KDA ...RNA POLYMERASES I AND III SUBUNIT AC1 / C37 / DNA-DIRECTED RN A POLYMERASES I AND III 40 KDA POLYPEPTIDE / AC40 / C40 / AC40


Mass: 37732.613 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) SACCHAROMYCES CEREVISIAE (brewer's yeast) / Strain: CB010 / References: UniProt: P07703
#14: Protein DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT RPAC2 / RNA POLYMERASES I AND III SUBUNIT AC2 / DNA-DIRECTED R NA POLYMERASES I AND III 16 KDA POLYPEPTIDE ...RNA POLYMERASES I AND III SUBUNIT AC2 / DNA-DIRECTED R NA POLYMERASES I AND III 16 KDA POLYPEPTIDE / RPA19 / AC19


Mass: 16167.860 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) SACCHAROMYCES CEREVISIAE (brewer's yeast) / Strain: CB010 / References: UniProt: P28000

-
Non-polymers , 3 types, 278 molecules

#15: Chemical
ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 14 / Source method: obtained synthetically / Formula: Zn
#16: Chemical ChemComp-SO4 / SULFATE ION / Sulfate


Mass: 96.063 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: SO4
#17: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 262 / Source method: isolated from a natural source / Formula: H2O

-
Experimental details

-
Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 4

-
Sample preparation

CrystalDensity Matthews: 2.85 Å3/Da / Density % sol: 56.9 % / Description: NONE
Crystal growpH: 7
Details: 10% PEG4000, 300MM AMMONIUM ACETATE, 50MM HEPES PH 7.5, 5MM TCEP

-
Data collection

Diffraction
IDMean temperature (K)Crystal-ID
11001
21001
Diffraction source
SourceSiteBeamlineIDWavelength
SYNCHROTRONSLS X10SA11
SYNCHROTRONSLS X06SA21.28288
Detector
TypeIDDetector
DECTRIS PILATUS 6M1PIXEL
DECTRIS PILATUS 6M2PIXEL
Radiation
IDProtocolMonochromatic (M) / Laue (L)Scattering typeWavelength-ID
1SINGLE WAVELENGTHMx-ray1
2SINGLE WAVELENGTHMx-ray1
Radiation wavelength
IDWavelength (Å)Relative weight
111
21.282881
ReflectionResolution: 2.8→40 Å / Num. obs: 320814 / % possible obs: 99.9 % / Observed criterion σ(I): 1.5 / Redundancy: 13.1 % / Rmerge(I) obs: 0.24 / Net I/σ(I): 9.61
Reflection shellResolution: 2.8→2.87 Å / Redundancy: 11.5 % / Mean I/σ(I) obs: 1.52 / % possible all: 99.8

-
Processing

Software
NameClassification
XDSdata reduction
XDSdata scaling
autoSHARPphasing
PARROTphasing
PHENIXrefinement
RefinementMethod to determine structure: MAD
Starting model: NONE

Resolution: 2.8→39.292 Å / SU ML: 0.39 / σ(F): 1.99 / Phase error: 23.43 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.2099 6493 2 %
Rwork0.1689 --
obs0.1697 320815 99.91 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Refinement stepCycle: LAST / Resolution: 2.8→39.292 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms68821 0 24 262 69107
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.0170209
X-RAY DIFFRACTIONf_angle_d1.24194762
X-RAY DIFFRACTIONf_dihedral_angle_d16.31826576
X-RAY DIFFRACTIONf_chiral_restr0.08410577
X-RAY DIFFRACTIONf_plane_restr0.00512274
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDNumberRefine-IDTypeRms dev position (Å)
11A625X-RAY DIFFRACTIONPOSITIONAL
12P625X-RAY DIFFRACTIONPOSITIONAL0.075
21A475X-RAY DIFFRACTIONPOSITIONAL
22P475X-RAY DIFFRACTIONPOSITIONAL0.037
31A783X-RAY DIFFRACTIONPOSITIONAL
32P783X-RAY DIFFRACTIONPOSITIONAL0.055
41A427X-RAY DIFFRACTIONPOSITIONAL
42P427X-RAY DIFFRACTIONPOSITIONAL0.092
51A141X-RAY DIFFRACTIONPOSITIONAL
52P141X-RAY DIFFRACTIONPOSITIONAL0.035
61A489X-RAY DIFFRACTIONPOSITIONAL
62P489X-RAY DIFFRACTIONPOSITIONAL0.101
71A279X-RAY DIFFRACTIONPOSITIONAL
72P279X-RAY DIFFRACTIONPOSITIONAL0.042
81A1367X-RAY DIFFRACTIONPOSITIONAL
82P1367X-RAY DIFFRACTIONPOSITIONAL0.041
91A1368X-RAY DIFFRACTIONPOSITIONAL
92P1368X-RAY DIFFRACTIONPOSITIONAL0.042
101A1220X-RAY DIFFRACTIONPOSITIONAL
102P1220X-RAY DIFFRACTIONPOSITIONAL0.042
111A316X-RAY DIFFRACTIONPOSITIONAL
112P316X-RAY DIFFRACTIONPOSITIONAL0.054
121A1012X-RAY DIFFRACTIONPOSITIONAL
122P1012X-RAY DIFFRACTIONPOSITIONAL0.041
131A341X-RAY DIFFRACTIONPOSITIONAL
132P341X-RAY DIFFRACTIONPOSITIONAL0.041
141A921X-RAY DIFFRACTIONPOSITIONAL
142P921X-RAY DIFFRACTIONPOSITIONAL0.043
151A290X-RAY DIFFRACTIONPOSITIONAL
152P290X-RAY DIFFRACTIONPOSITIONAL0.083
161A524X-RAY DIFFRACTIONPOSITIONAL
162P524X-RAY DIFFRACTIONPOSITIONAL0.048
171B544X-RAY DIFFRACTIONPOSITIONAL
172Q544X-RAY DIFFRACTIONPOSITIONAL0.038
181B885X-RAY DIFFRACTIONPOSITIONAL
182Q885X-RAY DIFFRACTIONPOSITIONAL0.039
191B1632X-RAY DIFFRACTIONPOSITIONAL
192Q1632X-RAY DIFFRACTIONPOSITIONAL0.05
201B677X-RAY DIFFRACTIONPOSITIONAL
202Q677X-RAY DIFFRACTIONPOSITIONAL0.092
211B1651X-RAY DIFFRACTIONPOSITIONAL
212Q1651X-RAY DIFFRACTIONPOSITIONAL0.068
221B787X-RAY DIFFRACTIONPOSITIONAL
222Q787X-RAY DIFFRACTIONPOSITIONAL0.045
231B865X-RAY DIFFRACTIONPOSITIONAL
232Q865X-RAY DIFFRACTIONPOSITIONAL0.03
241B1004X-RAY DIFFRACTIONPOSITIONAL
242Q1004X-RAY DIFFRACTIONPOSITIONAL0.055
251B1216X-RAY DIFFRACTIONPOSITIONAL
252Q1216X-RAY DIFFRACTIONPOSITIONAL0.047
261C377X-RAY DIFFRACTIONPOSITIONAL
262R377X-RAY DIFFRACTIONPOSITIONAL0.145
271C1121X-RAY DIFFRACTIONPOSITIONAL
272R1121X-RAY DIFFRACTIONPOSITIONAL0.048
281C915X-RAY DIFFRACTIONPOSITIONAL
282R915X-RAY DIFFRACTIONPOSITIONAL0.032
291D459X-RAY DIFFRACTIONPOSITIONAL
292S459X-RAY DIFFRACTIONPOSITIONAL0.029
301G890X-RAY DIFFRACTIONPOSITIONAL
302V890X-RAY DIFFRACTIONPOSITIONAL0.036
311G618X-RAY DIFFRACTIONPOSITIONAL
312V618X-RAY DIFFRACTIONPOSITIONAL0.058
321O404X-RAY DIFFRACTIONPOSITIONAL
3224404X-RAY DIFFRACTIONPOSITIONAL0.033
331E1161X-RAY DIFFRACTIONPOSITIONAL
332T1161X-RAY DIFFRACTIONPOSITIONAL0.027
341E565X-RAY DIFFRACTIONPOSITIONAL
342T565X-RAY DIFFRACTIONPOSITIONAL0.065
351F823X-RAY DIFFRACTIONPOSITIONAL
352U823X-RAY DIFFRACTIONPOSITIONAL0.037
361H1052X-RAY DIFFRACTIONPOSITIONAL
362W1052X-RAY DIFFRACTIONPOSITIONAL0.032
371I516X-RAY DIFFRACTIONPOSITIONAL
372X516X-RAY DIFFRACTIONPOSITIONAL0.043
381I384X-RAY DIFFRACTIONPOSITIONAL
382X384X-RAY DIFFRACTIONPOSITIONAL0.032
391J570X-RAY DIFFRACTIONPOSITIONAL
392Y570X-RAY DIFFRACTIONPOSITIONAL0.036
401K786X-RAY DIFFRACTIONPOSITIONAL
402Z786X-RAY DIFFRACTIONPOSITIONAL0.055
411L353X-RAY DIFFRACTIONPOSITIONAL
4121353X-RAY DIFFRACTIONPOSITIONAL0.029
421M814X-RAY DIFFRACTIONPOSITIONAL
4222814X-RAY DIFFRACTIONPOSITIONAL0.031
431N816X-RAY DIFFRACTIONPOSITIONAL
4323816X-RAY DIFFRACTIONPOSITIONAL0.031
441N320X-RAY DIFFRACTIONPOSITIONAL
4423320X-RAY DIFFRACTIONPOSITIONAL0.032
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.8-2.83180.34852120.31810451X-RAY DIFFRACTION100
2.8318-2.86510.33972180.310460X-RAY DIFFRACTION100
2.8651-2.90.32252360.285810483X-RAY DIFFRACTION100
2.9-2.93670.30652360.265410465X-RAY DIFFRACTION100
2.9367-2.97540.30112110.253910453X-RAY DIFFRACTION100
2.9754-3.01610.31462230.254910481X-RAY DIFFRACTION100
3.0161-3.05920.27032300.247610455X-RAY DIFFRACTION100
3.0592-3.10480.31742360.246910423X-RAY DIFFRACTION100
3.1048-3.15330.33042080.245910561X-RAY DIFFRACTION100
3.1533-3.2050.28172100.243110452X-RAY DIFFRACTION100
3.205-3.26020.27062480.225510466X-RAY DIFFRACTION100
3.2602-3.31950.29451980.220410572X-RAY DIFFRACTION100
3.3195-3.38330.25872010.210910465X-RAY DIFFRACTION100
3.3833-3.45230.24782170.199410411X-RAY DIFFRACTION100
3.4523-3.52740.2262270.189310479X-RAY DIFFRACTION100
3.5274-3.60940.23812060.185810565X-RAY DIFFRACTION100
3.6094-3.69950.2191970.169310428X-RAY DIFFRACTION100
3.6995-3.79950.21672150.162710469X-RAY DIFFRACTION100
3.7995-3.91120.20622090.159210507X-RAY DIFFRACTION100
3.9112-4.03730.21662170.152610449X-RAY DIFFRACTION100
4.0373-4.18150.20152100.147810538X-RAY DIFFRACTION100
4.1815-4.34870.17352150.133610527X-RAY DIFFRACTION100
4.3487-4.54630.15912060.129810456X-RAY DIFFRACTION100
4.5463-4.78560.16652200.125510513X-RAY DIFFRACTION100
4.7856-5.08490.16882270.126910452X-RAY DIFFRACTION100
5.0849-5.47650.15552230.133810483X-RAY DIFFRACTION100
5.4765-6.02590.1852000.143710500X-RAY DIFFRACTION100
6.0259-6.89380.1962040.149110504X-RAY DIFFRACTION100
6.8938-8.670.17112220.138210477X-RAY DIFFRACTION100
8.67-39.29630.15542110.146410377X-RAY DIFFRACTION99
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.8457-0.28590.10470.51880.02050.32640.01240.13860.3649-0.0644-0.0437-0.1187-0.14510.0404-00.5303-0.0410.00240.48750.09650.630449.5617129.4574160.767
20.7875-0.0413-0.05850.9547-0.28090.99060.0092-0.0915-0.12670.1126-0.03810.10660.13-0.0708-0.00010.4925-0.03030.00120.53230.04950.486615.469989.3582163.8487
31.3251-0.0103-0.38741.22510.08521.1674-0.01620.0912-0.4279-0.0799-0.077-0.23590.16470.1425-0.00020.52770.029-0.01110.58720.03230.633640.157869.4737136.6994
41.1614-0.0249-0.00471.00420.40491.20390.0580.8883-0.3307-0.5104-0.22570.19510.2821-0.3358-0.01090.88910.0662-0.20871.3082-0.1350.685.380681.6845107.1565
51.0481-0.2490.31121.06610.03450.7146-0.0843-0.5060.12750.56020.2702-0.6160.14310.31170.00020.89330.1725-0.30011.0172-0.14730.9531100.735994.2562206.2561
60.12550.02940.00450.08070.04470.0294-0.3526-0.5349-1.12980.25960.28520.31211.05090.1412-0.00171.08190.1868-0.07610.91050.19610.8685.549968.8476202.3236
70.06360.08330.04330.12520.10130.14870.0940.19020.393-0.1858-0.00790.3877-0.3311-0.55210.00010.97290.08490.02430.84460.17910.746145.293293.4795201.7095
80.08120.11110.09190.18970.17420.1661-0.15260.1593-0.0210.1446-0.36820.48750.3899-0.79020.00060.8157-0.10070.05290.8673-0.08130.757930.7226111.5067182.3912
90.52110.47420.3050.76170.21010.619-0.0249-0.0379-0.5020.00840.0499-0.19440.403-0.15360.00010.88540.0083-0.05510.79630.17720.684455.734667.7819203.1779
100.8446-0.4506-0.20610.81370.18870.9853-0.1423-0.0734-0.09460.16020.0743-0.11460.20810.12790.00010.59220.0288-0.06650.57870.04040.557279.910385.4226183.0671
110.4143-0.05940.24160.46460.19610.2566-0.1556-0.11760.19120.20330.2497-0.26270.11280.18470.00010.52680.0372-0.15030.6563-0.04540.632182.6974106.7574193.855
120.6955-0.1427-0.00110.76690.27390.68740.0244-0.31640.34120.1424-0.09640.379-0.305-0.3942-0.00020.74130.17140.08330.7904-0.15611.0088-3.01140.1705183.9891
130.79550.05630.11030.72330.23390.6128-0.049-0.11720.2240.0755-0.18510.4095-0.2239-0.1741-0.01140.75180.04590.03310.595-0.12290.788440.5961125.924754.8951
140.783-0.04730.00661.11740.59021.44260.00140.1705-0.12020.0727-0.0093-0.07130.15210.23840.00030.63660.00710.04330.63580.02510.472579.869390.325653.1921
151.3649-0.0962-0.30611.4060.08481.27280.0506-0.0261-0.4180.1393-0.13420.28910.2733-0.142-0.00030.8831-0.0720.02740.5854-0.00290.635557.100167.258179.3932
160.7712-0.00770.23570.294-0.70511.54060.088-0.3952-0.18250.0414-0.107-0.02070.30590.36310.00011.0382-0.0027-0.08780.97250.040.57288.569385.1618111.0841
170.86790.37480.22711.03440.26150.61-0.11130.3664-0.0835-0.4326-0.36120.9151-0.1769-0.5728-0.02620.83750.1021-0.23461.4629-0.65121.7693-4.163883.51528.1337
180.1241-0.11460.17010.1447-0.22260.34420.1456-0.174-0.82690.0087-0.29110.58690.6624-0.05110.02220.8221-0.09760.05921.2175-0.68471.731713.652460.301813.3168
190.0667-0.0593-0.00070.1026-0.07110.10040.05780.03280.17410.2879-0.25020.1639-0.210.12430.00110.7564-0.047-0.09080.8881-0.32070.917948.112487.195414.4203
200.0859-0.0350.08720.1205-0.09280.1181-0.1485-0.1992-0.24310.0328-0.3464-0.29520.18710.69430.0011.09810.07110.03441.00960.04980.93262.0321109.828134.3912
210.6127-0.52380.39190.5793-0.28780.6648-0.0592-0.1298-0.60070.007-0.11360.57620.38850.19470.00030.72-0.00130.0620.9038-0.31231.087543.413362.787213.4834
220.4579-0.01040.05980.64830.03770.6098-0.0896-0.0377-0.33570.1852-0.25490.51630.2106-0.30540.00030.6041-0.09760.16270.8681-0.38611.296916.255577.795732.5526
230.1873-0.1143-0.06640.2175-0.15660.2894-0.22010.1501-0.0121-0.1077-0.12490.602-0.0463-0.5024-0.02140.54310.1145-0.19790.9463-0.44441.302411.422698.388121.3402
240.67020.15410.12660.597-0.25980.6047-0.03220.50910.5471-0.2255-0.0115-0.1849-0.5540.4894-0.00021.1027-0.30280.04030.9030.20320.783691.5535142.785232.9817
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1CHAIN A AND (RESID 654:1013 OR RESID 1361:1367) OR CHAIN B AND (RESID 8:209 OR RESID 400:553 OR RESID 647:1068) OR CHAIN C OR CHAIN J OR CHAIN K OR CHAIN L OR CHAIN N AND RESID 147:180
2X-RAY DIFFRACTION2CHAIN A AND (RESID 477:653 OR RESID 1014:1261 OR RESID 1368:1395 OR RESID 1500:1608) OR CHAIN B AND RESID 1069:1092 OR CHAIN E OR CHAIN F AND RESID 73:154
3X-RAY DIFFRACTION3CHAIN A AND (RESID 1:476 OR RESID 1609:1649 OR RESID 3001:3002) OR CHAIN B AND (RESID 1093-1203 OR RESID 3001) OR CHAIN O
4X-RAY DIFFRACTION4CHAIN A AND RESID 1650:1664 OR CHAIN D OR CHAIN F AND RESID 55:72 OR CHAIN G
5X-RAY DIFFRACTION5CHAIN M OR CHAIN N AND RESID 25:146
6X-RAY DIFFRACTION6CHAIN I AND (RESID 2:52 OR RESID 3001)
7X-RAY DIFFRACTION7CHAIN I AND RESID 53:78
8X-RAY DIFFRACTION8CHAIN I AND (RESID 79:125 OR RESID 3002)
9X-RAY DIFFRACTION9CHAIN A AND (RESID 1262:1337 OR RESID 1440:1499)
10X-RAY DIFFRACTION10CHAIN B AND RESID 210:399
11X-RAY DIFFRACTION11CHAIN B AND RESID 554:646
12X-RAY DIFFRACTION12CHAIN H
13X-RAY DIFFRACTION13CHAIN P AND (RESID 654:1013 OR RESID 1361:1367) OR CHAIN Q AND (RESID 8:209 OR RESID 400:553 OR RESID 647:1068) OR CHAIN R OR CHAIN Y OR CHAIN Z OR CHAIN 1 OR CHAIN 3 AND RESID 147:181
14X-RAY DIFFRACTION14CHAIN P AND (RESID 477:653 OR RESID 1014:1261 OR RESID 1368:1395 OR RESID 1500:1608) OR CHAIN Q AND RESID 1069:1092 OR CHAIN T OR CHAIN U AND RESID 73:154
15X-RAY DIFFRACTION15CHAIN P AND (RESID 1:476 OR RESID 1609:1649 OR RESID 3001:3002) OR CHAIN Q AND (RESID 1093-1203 OR RESID 3001) OR CHAIN 4
16X-RAY DIFFRACTION16CHAIN P AND RESID 1650:1664 OR CHAIN S OR CHAIN U AND RESID 55:72 OR CHAIN V
17X-RAY DIFFRACTION17CHAIN 2 OR CHAIN 3 AND RESID 25:146
18X-RAY DIFFRACTION18CHAIN X AND (RESID 2:52 OR RESID 3001)
19X-RAY DIFFRACTION19CHAIN X AND RESID 53:78
20X-RAY DIFFRACTION20CHAIN X AND (RESID 79:125 OR RESID 3002)
21X-RAY DIFFRACTION21CHAIN P AND (RESID 1262:1337 OR RESID 1440:1499)
22X-RAY DIFFRACTION22CHAIN Q AND RESID 210:399
23X-RAY DIFFRACTION23CHAIN Q AND RESID 554:646
24X-RAY DIFFRACTION24CHAIN W

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