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- EMDB-31211: Human Mediator (deletion of MED1-IDR) in a Tail-bent conformation... -

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Basic information

Entry
Database: EMDB / ID: EMD-31211
TitleHuman Mediator (deletion of MED1-IDR) in a Tail-bent conformation (MED-B)
Map data
Sample
  • Complex: Structure of the human Mediator complex
    • Protein or peptide: x 26 types
  • Ligand: x 1 types
Function / homology
Function and homology information


positive regulation of T cell extravasation / negative regulation of smooth muscle cell differentiation / enucleate erythrocyte development / positive regulation of type II interferon-mediated signaling pathway / mammary gland branching involved in thelarche / regulation of RNA biosynthetic process / androgen biosynthetic process / positive regulation of G0 to G1 transition / retinal pigment epithelium development / G0 to G1 transition ...positive regulation of T cell extravasation / negative regulation of smooth muscle cell differentiation / enucleate erythrocyte development / positive regulation of type II interferon-mediated signaling pathway / mammary gland branching involved in thelarche / regulation of RNA biosynthetic process / androgen biosynthetic process / positive regulation of G0 to G1 transition / retinal pigment epithelium development / G0 to G1 transition / thyroid hormone mediated signaling pathway / core mediator complex / regulation of vitamin D receptor signaling pathway / mediator complex / nuclear retinoic acid receptor binding / ventricular trabecula myocardium morphogenesis / thyroid hormone generation / positive regulation of keratinocyte differentiation / Generic Transcription Pathway / embryonic heart tube development / positive regulation of hepatocyte proliferation / cellular response to thyroid hormone stimulus / embryonic hindlimb morphogenesis / peroxisome proliferator activated receptor binding / nuclear vitamin D receptor binding / limb development / lens development in camera-type eye / nuclear thyroid hormone receptor binding / embryonic hemopoiesis / positive regulation of intracellular estrogen receptor signaling pathway / histone acetyltransferase binding / megakaryocyte development / erythrocyte development / termination of RNA polymerase II transcription / cellular response to hepatocyte growth factor stimulus / cellular response to steroid hormone stimulus / cortical actin cytoskeleton / epithelial cell proliferation involved in mammary gland duct elongation / LBD domain binding / fat cell differentiation / skeletal muscle cell differentiation / mammary gland branching involved in pregnancy / somatic stem cell population maintenance / monocyte differentiation / blastocyst development / general transcription initiation factor binding / negative regulation of neuron differentiation / positive regulation of transcription initiation by RNA polymerase II / hematopoietic stem cell differentiation / embryonic placenta development / negative regulation of keratinocyte proliferation / animal organ regeneration / ubiquitin ligase complex / intracellular steroid hormone receptor signaling pathway / negative regulation of fibroblast proliferation / RNA polymerase II preinitiation complex assembly / keratinocyte differentiation / cellular response to epidermal growth factor stimulus / peroxisome proliferator activated receptor signaling pathway / RORA activates gene expression / lactation / Regulation of lipid metabolism by PPARalpha / BMAL1:CLOCK,NPAS2 activates circadian gene expression / nuclear receptor coactivator activity / Activation of gene expression by SREBF (SREBP) / positive regulation of erythrocyte differentiation / liver development / nuclear estrogen receptor binding / promoter-specific chromatin binding / transcription initiation at RNA polymerase II promoter / nuclear receptor binding / positive regulation of transcription elongation by RNA polymerase II / transcription coregulator activity / brain development / Heme signaling / mRNA transcription by RNA polymerase II / cell morphogenesis / Transcriptional activation of mitochondrial biogenesis / transcription coactivator binding / PPARA activates gene expression / Cytoprotection by HMOX1 / chromatin DNA binding / DNA-directed 5'-3' RNA polymerase activity / Transcriptional regulation of white adipocyte differentiation / Nuclear Receptor transcription pathway / protein import into nucleus / transcription corepressor activity / ubiquitin protein ligase activity / Circadian Clock / actin binding / angiogenesis / DNA-binding transcription factor binding / Estrogen-dependent gene expression / transcription regulator complex / transcription by RNA polymerase II / transcription coactivator activity / nuclear body / protein ubiquitination / RNA polymerase II cis-regulatory region sequence-specific DNA binding / DNA-templated transcription
Similarity search - Function
Mediator complex subunit 26, C-terminal domain / Mediator complex subunit 26, middle domain / Mediator of RNA polymerase II transcription subunit 26 / : / Mediator complex subunit 26 C-terminal / Mediator complex subunit 26 middle domain / Mediator complex subunit 16, central helical bridge / : / : / ARC105/Med15 mediator subunit central domain ...Mediator complex subunit 26, C-terminal domain / Mediator complex subunit 26, middle domain / Mediator of RNA polymerase II transcription subunit 26 / : / Mediator complex subunit 26 C-terminal / Mediator complex subunit 26 middle domain / Mediator complex subunit 16, central helical bridge / : / : / ARC105/Med15 mediator subunit central domain / ARC105/Med15 mediator subunit C-terminal domain / Mediator complex, subunit Med29, metazoa / Mediator complex, subunit Med24, / Mediator complex subunit 24 N-terminal / Mediator complex subunit 29 / Mediator of RNA polymerase II transcription subunit 15 / Mediator complex, subunit Med23 / ARC105/Med15 mediator subunit N-terminal KIX domain / Mediator complex subunit 23 / Mediator complex subunit 28 / Mediator complex, subunit Med6, metazoa/plant / Mediator complex, subunit Med19, metazoa / Mediator complex, subunit Med30, metazoa / Mediator complex, subunit Med28 / Mediator of RNA polymerase II transcription subunit 9, metazoan / Mediator of RNA pol II transcription subunit 19 / Mediator complex subunit 30 / : / : / Mediator complex subunit 16, C-terminal / Mediator complex, subunit Med27 / Mediator complex subunit 27 / Mediator complex, subunit Med16 / Mediator complex subunit 16, N-terminal / Mediator complex, subunit Med10 / Transcription factor subunit Med10 of Mediator complex / Mediator of RNA polymerase II transcription subunit 9 / RNA polymerase II transcription mediator complex subunit 9 / Mediator complex, subunit Med4 / Vitamin-D-receptor interacting Mediator subunit 4 / Mediator complex, subunit Med7 superfmaily / Mediator complex, subunit Med31 / Mediator complex, subunit Med21 / Mediator of RNA polymerase II, subunit Med31 superfamily / SOH1 / Subunit 21 of Mediator complex / Mediator complex, subunit Med7 / Soluble quinoprotein glucose/sorbosone dehydrogenase / Mediator complex, subunit Med7/Med21-like / MED7 protein / Mediator complex, subunit Med20 / Mediator complex, subunit Med14 / TATA-binding related factor (TRF) of subunit 20 of Mediator complex / Mediator complex subunit MED14 / Mediator complex, subunit Med6 / Mediator complex, subunit Med17 / Mediator complex, subunit Med6 superfamily / MED6 mediator sub complex component / Subunit 17 of Mediator complex / Mediator of RNA polymerase II transcription subunit 22 / Mediator complex, subunit Med8, fungi/metazoa / Mediator complex, subunit Med11 / Surfeit locus protein 5 subunit 22 of Mediator complex / Mediator of RNA polymerase II transcription complex subunit 8 / Mediator complex protein / Mediator complex, subunit Med18 / Med18 protein / Transcription elongation factor, TFIIS/CRSP70, N-terminal, sub-type / Domain in the N-terminus of transcription elongation factor S-II (and elsewhere) / Mediator complex, subunit Med1 / Mediator of RNA polymerase II transcription subunit 1 / TFIIS helical bundle-like domain / Transcription factor IIS, N-terminal / TFIIS N-terminal domain profile. / TFIIS/LEDGF domain superfamily / Coactivator CBP, KIX domain superfamily / WD domain, G-beta repeat / WD40 repeats / WD40 repeat / Trp-Asp (WD) repeats profile. / Trp-Asp (WD) repeats circular profile. / WD40-repeat-containing domain superfamily / WD40/YVTN repeat-like-containing domain superfamily
Similarity search - Domain/homology
Mediator of RNA polymerase II transcription subunit 19 / Mediator of RNA polymerase II transcription subunit 7 / Mediator of RNA polymerase II transcription subunit 14 / Mediator of RNA polymerase II transcription subunit 24 / Mediator of RNA polymerase II transcription subunit 6 / Mediator of RNA polymerase II transcription subunit 26 / Mediator of RNA polymerase II transcription subunit 21 / Mediator of RNA polymerase II transcription subunit 22 / Mediator of RNA polymerase II transcription subunit 1 / Mediator of RNA polymerase II transcription subunit 27 ...Mediator of RNA polymerase II transcription subunit 19 / Mediator of RNA polymerase II transcription subunit 7 / Mediator of RNA polymerase II transcription subunit 14 / Mediator of RNA polymerase II transcription subunit 24 / Mediator of RNA polymerase II transcription subunit 6 / Mediator of RNA polymerase II transcription subunit 26 / Mediator of RNA polymerase II transcription subunit 21 / Mediator of RNA polymerase II transcription subunit 22 / Mediator of RNA polymerase II transcription subunit 1 / Mediator of RNA polymerase II transcription subunit 27 / Mediator of RNA polymerase II transcription subunit 8 / Mediator of RNA polymerase II transcription subunit 30 / Mediator of RNA polymerase II transcription subunit 15 / Mediator of RNA polymerase II transcription subunit 10 / Mediator of RNA polymerase II transcription subunit 18 / Mediator of RNA polymerase II transcription subunit 28 / Mediator of RNA polymerase II transcription subunit 20 / Mediator of RNA polymerase II transcription subunit 4 / Mediator of RNA polymerase II transcription subunit 17 / Mediator of RNA polymerase II transcription subunit 9 / Mediator of RNA polymerase II transcription subunit 29 / Mediator of RNA polymerase II transcription subunit 11 / Mediator of RNA polymerase II transcription subunit 23 / Mediator of RNA polymerase II transcription subunit 16 / Mediator of RNA polymerase II transcription subunit 31
Similarity search - Component
Biological speciesHomo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 4.4 Å
AuthorsYin X / Li J / Wu Z / Liu W / Xu Y
CitationJournal: Science / Year: 2021
Title: Structures of the human Mediator and Mediator-bound preinitiation complex.
Authors: Xizi Chen / Xiaotong Yin / Jiabei Li / Zihan Wu / Yilun Qi / Xinxin Wang / Weida Liu / Yanhui Xu /
Abstract: The 1.3-megadalton transcription factor IID (TFIID) is required for preinitiation complex (PIC) assembly and RNA polymerase II (Pol II)-mediated transcription initiation on almost all genes. The 26- ...The 1.3-megadalton transcription factor IID (TFIID) is required for preinitiation complex (PIC) assembly and RNA polymerase II (Pol II)-mediated transcription initiation on almost all genes. The 26-subunit Mediator stimulates transcription and cyclin-dependent kinase 7 (CDK7)-mediated phosphorylation of the Pol II C-terminal domain (CTD). We determined the structures of human Mediator in the Tail module-extended (at near-atomic resolution) and Tail-bent conformations and structures of TFIID-based PIC-Mediator (76 polypeptides, ~4.1 megadaltons) in four distinct conformations. PIC-Mediator assembly induces concerted reorganization (Head-tilting and Middle-down) of Mediator and creates a Head-Middle sandwich, which stabilizes two CTD segments and brings CTD to CDK7 for phosphorylation; this suggests a CTD-gating mechanism favorable for phosphorylation. The TFIID-based PIC architecture modulates Mediator organization and TFIIH stabilization, underscoring the importance of TFIID in orchestrating PIC-Mediator assembly.
History
DepositionApr 17, 2021-
Header (metadata) releaseMay 19, 2021-
Map releaseMay 19, 2021-
UpdateJun 16, 2021-
Current statusJun 16, 2021Processing site: PDBj / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.03
  • Imaged by UCSF Chimera
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  • Surface view colored by radius
  • Surface level: 0.03
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-7enj
  • Surface level: 0.03
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_31211.png

Downloads & links

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Map

FileDownload / File: emd_31211.map.gz / Format: CCP4 / Size: 64 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Voxel sizeX=Y=Z: 2.108 Å
Density
Contour LevelBy AUTHOR: 0.025 / Movie #1: 0.03
Minimum - Maximum-0.19444914 - 0.4518145
Average (Standard dev.)0.00016031876 (±0.008563838)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions256256256
Spacing256256256
CellA=B=C: 539.648 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z2.1082.1082.108
M x/y/z256256256
origin x/y/z0.0000.0000.000
length x/y/z539.648539.648539.648
α/β/γ90.00090.00090.000
start NX/NY/NZ000
NX/NY/NZ512512512
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS256256256
D min/max/mean-0.1940.4520.000

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Supplemental data

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Sample components

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Entire : Structure of the human Mediator complex

EntireName: Structure of the human Mediator complex
Components
  • Complex: Structure of the human Mediator complex
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 1
    • Protein or peptide: Unknown Chain
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 4
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 6
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 7
    • Protein or peptide: Isoform 2 of Mediator of RNA polymerase II transcription subunit 8
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 9
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 10
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 11
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 14
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 15
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 16
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 17
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 18
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 19
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 20
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 21
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 22
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 23
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 24
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 26
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 27
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 28
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 29
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 30
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 31
  • Ligand: ZINC ION

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Supramolecule #1: Structure of the human Mediator complex

SupramoleculeName: Structure of the human Mediator complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#26
Source (natural)Organism: Homo sapiens (human)
Recombinant expressionOrganism: Homo sapiens (human) / Recombinant cell: Expi293

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Macromolecule #1: Mediator of RNA polymerase II transcription subunit 1

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 1 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 168.697328 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MKAQGETEES EKLSKMSSLL ERLHAKFNQN RPWSETIKLV RQVMEKRVVM SSGGHQHLVS CLETLQKALK VTSLPAMTDR LESIARQNG LGSHLSASGT ECYITSDMFY VEVQLDPAGQ LCDVKVAHHG ENPVSCPELV QQLREKNFDE FSKHLKGLVN L YNLPGDNK ...String:
MKAQGETEES EKLSKMSSLL ERLHAKFNQN RPWSETIKLV RQVMEKRVVM SSGGHQHLVS CLETLQKALK VTSLPAMTDR LESIARQNG LGSHLSASGT ECYITSDMFY VEVQLDPAGQ LCDVKVAHHG ENPVSCPELV QQLREKNFDE FSKHLKGLVN L YNLPGDNK LKTKMYLALQ SLEQDLSKMA IMYWKATNAG PLDKILHGSV GYLTPRSGGH LMNLKYYVSP SDLLDDKTAS PI ILHENNV SRSLGMNASV TIEGTSAVYK LPIAPLIMGS HPVDNKWTPS FSSITSANSV DLPACFFLKF PQPIPVSRAF VQK LQNCTG IPLFETQPTY APLYELITQF ELSKDPDPIP LNHNMRFYAA LPGQQHCYFL NKDAPLPDGR SLQGTLVSKI TFQH PGRVP LILNLIRHQV AYNTLIGSCV KRTILKEDSP GLLQFEVCPL SESRFSVSFQ HPVNDSLVCV VMDVQDSTHV SCKLY KGLS DALICTDDFI AKVVQRCMSI PVTMRAIRRK AETIQADTPA LSLIAETVED MVKKNLPPAS SPGYGMTTGN NPMSGT TTP TNTFPGGPIT TLFNMSMSIK DRHESVGHGE DFSKVSQNPI LTSLLQITGN GGSTIGSSPT PPHHTPPPVS SMAGNTK NH PMLMNLLKDN PAQDFSTLYG SSPLERQNSS SGSPRMEICS GSNKTKKKKS SRLPPEKPKH QTEDDFQREL FSMDVDSQ N PIFDVNMTAD TLDTPHITPA PSQCSTPPTT YPQPVPHPQP SIQRMVRLSS SDSIGPDVTD ILSDIAEEAS KLPSTSDDC PAIGTPLRDS SSSGHSQSTL FDSDVFQTNN NENPYTDPAD LIADAAGSPS SDSPTNHFFH DGVDFNPDLL NSQSQSGFGE EYFDESSQS GDNDDFKGFA SQALNTLGVP MLGGDNGETK FKGNNQADTV DFSIISVAGK ALAPADLMEH HSGSQGPLLT T GDLGKEKT QKRVKEGNGT SNSTLSGPGL DSKPGKRSRT PSNDGKSKDK PPKRKKADTE GKSPSHSSSN RPFTPPTSTG GS KSPGSAG RSQTPPGVAT PPIPKITIQI PKGTVMVGKP SSHSQYTSSG SVSSSGSKSH HSHSSSSSSS ASTSGKMKSS KSE GSSSSK LSSSMYSSQG SSGSSQSKNS SQSGGKPGSS PITKHGLSSG SSSTKMKPQG KPSSLMNPSL SKPNISPSHS RPPG GSDKL ASPMKPVPGT PPSSKAKSPI SSGSGGSHMS GTSSSSGMKS SSGLGSSGSL SQKTPPSSNS CTASSSSFSS SGSSM SSSQ NQHGSSKGKS PSRNKKPSLT AVIDKLKHGV VTSGPGGEDP LDGQMGVSTN SSSHPMSSKH NMSGGEFQGK REKSDK DKS KVSTSGSSVD SSKKTSESKN VGSTGVAKII ISKHDGGSPS IKAKVTLQKP GESSGEGLRP QMASSKNYGS PLISGST PK HERGSPSHSK SPAYTPQNLD SESESGSSIA EKSYQNSPSS DDGIRPLPEY STEKHKKHKK EKKKVKDKDR DRDRDKDR D KKKSHSIKPE SWSKSPISSD QSLSMTSNTI LSADRPSRLS PDFMIGEEDD DLMDVALIGN

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Macromolecule #2: Unknown Chain

MacromoleculeName: Unknown Chain / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 1.720111 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)

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Macromolecule #3: Mediator of RNA polymerase II transcription subunit 4

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 4 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 29.781424 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MAASSSGEKE KERLGGGLGV AGGNSTRERL LSALEDLEVL SRELIEMLAI SRNQKLLQAG EENQVLELLI HRDGEFQELM KLALNQGKI HHEMQVLEKE VEKRDSDIQQ LQKQLKEAEQ ILATAVYQAK EKLKSIEKAR KGAISSEEII KYAHRISASN A VCAPLTWV ...String:
MAASSSGEKE KERLGGGLGV AGGNSTRERL LSALEDLEVL SRELIEMLAI SRNQKLLQAG EENQVLELLI HRDGEFQELM KLALNQGKI HHEMQVLEKE VEKRDSDIQQ LQKQLKEAEQ ILATAVYQAK EKLKSIEKAR KGAISSEEII KYAHRISASN A VCAPLTWV PGDPRRPYPT DLEMRSGLLG QMNNPSTNGV NGHLPGDALA AGRLPDVLAP QYPWQSNDMS MNMLPPNHSS DF LLEPPGH NKENEDDVEI MSTDSSSSSS ESD

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Macromolecule #4: Mediator of RNA polymerase II transcription subunit 6

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 6 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 28.464627 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MAAVDIRDNL LGISWVDSSW IPILNSGSVL DYFSERSNPF YDRTCNNEVV KMQRLTLEHL NQMVGIEYIL LHAQEPILFI IRKQQRQSP AQVIPLADYY IIAGVIYQAP DLGSVINSRV LTAVHGIQSA FDEAMSYCRY HPSKGYWWHF KDHEEQDKVR P KAKRKEEP ...String:
MAAVDIRDNL LGISWVDSSW IPILNSGSVL DYFSERSNPF YDRTCNNEVV KMQRLTLEHL NQMVGIEYIL LHAQEPILFI IRKQQRQSP AQVIPLADYY IIAGVIYQAP DLGSVINSRV LTAVHGIQSA FDEAMSYCRY HPSKGYWWHF KDHEEQDKVR P KAKRKEEP SSIFQRQRVD ALLLDLRQKF PPKFVQLKPG EKPVPVDQTK KEAEPIPETV KPEEKETTKN VQQTVSAKGP PE KRMRLQ

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Macromolecule #5: Mediator of RNA polymerase II transcription subunit 7

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 7 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 27.285281 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MGEPQQVSAL PPPPMQYIKE YTDENIQEGL APKPPPPIKD SYMMFGNQFQ CDDLIIRPLE SQGIERLHPM QFDHKKELRK LNMSILINF LDLLDILIRS PGSIKREEKL EDLKLLFVHV HHLINEYRPH QARETLRVMM EVQKRQRLET AERFQKHLER V IEMIQNCL ...String:
MGEPQQVSAL PPPPMQYIKE YTDENIQEGL APKPPPPIKD SYMMFGNQFQ CDDLIIRPLE SQGIERLHPM QFDHKKELRK LNMSILINF LDLLDILIRS PGSIKREEKL EDLKLLFVHV HHLINEYRPH QARETLRVMM EVQKRQRLET AERFQKHLER V IEMIQNCL ASLPDDLPHS EAGMRVKTEP MDADDSNNCT GQNEHQRENS GHRRDQIIEK DAALCVLIDE MNERP

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Macromolecule #6: Isoform 2 of Mediator of RNA polymerase II transcription subunit 8

MacromoleculeName: Isoform 2 of Mediator of RNA polymerase II transcription subunit 8
type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 29.112906 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MQREEKQLEA SLDALLSQVA DLKNSLGSFI CKLENEYGRL TWPSVLDSFA LLSGQLNTLN KVLKHEKTPL FRNQVIIPLV LSPDRDEDL MRQTEGRVPV FSHEVVPDHL RTKPDPEVEE QEKQLTTDAA RIGADAAQKQ IQSLNKMCSN LLEKISKEER E SESGGLRP ...String:
MQREEKQLEA SLDALLSQVA DLKNSLGSFI CKLENEYGRL TWPSVLDSFA LLSGQLNTLN KVLKHEKTPL FRNQVIIPLV LSPDRDEDL MRQTEGRVPV FSHEVVPDHL RTKPDPEVEE QEKQLTTDAA RIGADAAQKQ IQSLNKMCSN LLEKISKEER E SESGGLRP NKQTFNPTDT NALVAAVAFG KGLSNWRPSG SSGPGQAGQP GAGTILAGTS GLQQVQMAGA PSQQQPMLSG VQ MAQAGQP GKMPSGIKTN IKSASMHPYQ R

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Macromolecule #7: Mediator of RNA polymerase II transcription subunit 9

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 9 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 16.42275 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString:
MASAGVAAGR QAEDVLPPTS DQPLPDTKPL PPPQPPPVPA PQPQQSPAPR PQSPARAREE ENYSFLPLVH NIIKCMDKDS PEVHQDLNA LKSKFQEMRK LISTMPGIHL SPEQQQQQLQ SLREQVRTKN ELLQKYKSLC MFEIPKE

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Macromolecule #8: Mediator of RNA polymerase II transcription subunit 10

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 10
type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 15.710969 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString:
MAEKFDHLEE HLEKFVENIR QLGIIVSDFQ PSSQAGLNQK LNFIVTGLQD IDKCRQQLHD ITVPLEVFEY IDQGRNPQLY TKECLERAL AKNEQVKGKI DTMKKFKSLL IQELSKVFPE DMAKYRSIRG EDHPPS

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Macromolecule #9: Mediator of RNA polymerase II transcription subunit 11

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 11
type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 13.145829 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString:
MATYSLANER LRALEDIERE IGAILQNAGT VILELSKEKT NERLLDRQAA AFTASVQHVE AELSAQIRYL TQVATGQPHE GSSYSSRKD CQMALKRVDY ARLKLSDVAR TCEQMLEN

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Macromolecule #10: Mediator of RNA polymerase II transcription subunit 14

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 14
type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 160.787594 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MAPVQLENHQ LVPPGGGGGG SGGPPSAPAP PPPGAAVAAA AAAAASPGYR LSTLIEFLLH RAYSELMVLT DLLPRKSDVE RKIEIVQFA SRTRQLFVRL LALVKWANNA GKVEKCAMIS SFLDQQAILF VDTADRLASL ARDALVHARL PSFAIPYAID V LTTGSYPR ...String:
MAPVQLENHQ LVPPGGGGGG SGGPPSAPAP PPPGAAVAAA AAAAASPGYR LSTLIEFLLH RAYSELMVLT DLLPRKSDVE RKIEIVQFA SRTRQLFVRL LALVKWANNA GKVEKCAMIS SFLDQQAILF VDTADRLASL ARDALVHARL PSFAIPYAID V LTTGSYPR LPTCIRDKII PPDPITKIEK QATLHQLNQI LRHRLVTTDL PPQLANLTVA NGRVKFRVEG EFEATLTVMG DD PDVPWRL LKLEILVEDK ETGDGRALVH SMQISFIHQL VQSRLFADEK PLQDMYNCLH SFCLSLQLEV LHSQTLMLIR ERW GDLVQV ERYHAGKCLS LSVWNQQVLG RKTGTASVHK VTIKIDENDV SKPLQIFHDP PLPASDSKLV ERAMKIDHLS IEKL LIDSV HARAHQKLQE LKAILRGFNA NENSSIETAL PALVVPILEP CGNSECLHIF VDLHSGMFQL MLYGLDQATL DDMEK SVND DMKRIIPWIQ QLKFWLGQQR CKQSIKHLPT ISSETLQLSN YSTHPIGNLS KNKLFIKLTR LPQYYIVVEM LEVPNK PTQ LSYKYYFMSV NAADREDSPA MALLLQQFKE NIQDLVFRTK TGKQTRTNAK RKLSDDPCPV ESKKTKRAGE MCAFNKV LA HFVAMCDTNM PFVGLRLELS NLEIPHQGVQ VEGDGFSHAI RLLKIPPCKG ITEETQKALD RSLLDCTFRL QGRNNRTW V AELVFANCPL NGTSTREQGP SRHVYLTYEN LLSEPVGGRK VVEMFLNDWN SIARLYECVL EFARSLPDIP AHLNIFSEV RVYNYRKLIL CYGTTKGSSI SIQWNSIHQK FHISLGTVGP NSGCSNCHNT ILHQLQEMFN KTPNVVQLLQ VLFDTQAPLN AINKLPTVP MLGLTQRTNT AYQCFSILPQ SSTHIRLAFR NMYCIDIYCR SRGVVAIRDG AYSLFDNSKL VEGFYPAPGL K TFLNMFVD SNQDARRRSV NEDDNPPSPI GGDMMDSLIS QLQPPPQQQP FPKQPGTSGA YPLTSPPTSY HSTVNQSPSM MH TQSPGNL HAASSPSGAL RAPSPASFVP TPPPSSHGIS IGPGASFASP HGTLDPSSPY TMVSPSGRAG NWPGSPQVSG PSP AARMPG MSPANPSLHS PVPDASHSPR AGTSSQTMPT NMPPPRKLPQ RSWAASIPTI LTHSALNILL LPSPTPGLVP GLAG SYLCS PLERFLGSVI MRRHLQRIIQ QETLQLINSN EPGVIMFKTD ALKCRVALSP KTNQTLQLKV TPENAGQWKP DELQV LEKF FETRVAGPPF KANTLIAFTK LLGAPTHILR DCVHIMKLEL FPDQATQLKW NVQFCLTIPP SAPPIAPPGT PAVVLK SKM LFFLQLTQKT SVPPQEPVSI IVPIIYDMAS GTTQQADIPR QQNSSVAAPM MVSNILKRFA EMNPPRQGEC TIFAAVR DL MANLTLPPGG RP

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Macromolecule #11: Mediator of RNA polymerase II transcription subunit 15

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 15
type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 86.830102 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MDVSGQETDW RSTAFRQKLV SQIEDAMRKA GVAHSKSSKD MESHVFLKAK TRDEYLSLVA RLIIHFRDIH NKKSQASVSD PMNALQSLT GGPAAGAAGI GMPPRGPGQS LGGMGSLGAM GQPMSLSGQP PPGTSGMAPH SMAVVSTATP QTQLQLQQVA L QQQQQQQQ ...String:
MDVSGQETDW RSTAFRQKLV SQIEDAMRKA GVAHSKSSKD MESHVFLKAK TRDEYLSLVA RLIIHFRDIH NKKSQASVSD PMNALQSLT GGPAAGAAGI GMPPRGPGQS LGGMGSLGAM GQPMSLSGQP PPGTSGMAPH SMAVVSTATP QTQLQLQQVA L QQQQQQQQ FQQQQQAALQ QQQQQQQQQQ FQAQQSAMQQ QFQAVVQQQQ QLQQQQQQQQ HLIKLHHQNQ QQIQQQQQQL QR IAQLQLQ QQQQQQQQQQ QQQQQALQAQ PPIQQPPMQQ PQPPPSQALP QQLQQMHHTQ HHQPPPQPQQ PPVAQNQPSQ LPP QSQTQP LVSQAQALPG QMLYTQPPLK FVRAPMVVQQ PPVQPQVQQQ QTAVQTAQAA QMVAPGVQMI TEALAQGGMH IRAR FPPTT AVSAIPSSSI PLGRQPMAQV SQSSLPMLSS PSPGQQVQTP QSMPPPPQPS PQPGQPSSQP NSNVSSGPAP SPSSF LPSP SPQPSQSPVT ARTPQNFSVP SPGPLNTPVN PSSVMSPAGS SQAEEQQYLD KLKQLSKYIE PLRRMINKID KNEDRK KDL SKMKSLLDIL TDPSKRCPLK TLQKCEIALE KLKNDMAVPT PPPPPVPPTK QQYLCQPLLD AVLANIRSPV FNHSLYR TF VPAMTAIHGP PITAPVVCTR KRRLEDDERQ SIPSVLQGEV ARLDPKFLVN LDPSHCSNNG TVHLICKLDD KDLPSVPP L ELSVPADYPA QSPLWIDRQW QYDANPFLQS VHRCMTSRLL QLPDKHSVTA LLNTWAQSVH QACLSAA

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Macromolecule #12: Mediator of RNA polymerase II transcription subunit 16

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 16
type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 96.908055 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MCDLRRPAAG GMMDLAYVCE WEKWSKSTHC PSVPLACAWS CRNLIAFTMD LRSDDQDLTR MIHILDTEHP WDLHSIPSEH HEAITCLEW DQSGSRLLSA DADGQIKCWS MADHLANSWE SSVGSLVEGD PIVALSWLHN GVKLALHVEK SGASSFGEKF S RVKFSPSL ...String:
MCDLRRPAAG GMMDLAYVCE WEKWSKSTHC PSVPLACAWS CRNLIAFTMD LRSDDQDLTR MIHILDTEHP WDLHSIPSEH HEAITCLEW DQSGSRLLSA DADGQIKCWS MADHLANSWE SSVGSLVEGD PIVALSWLHN GVKLALHVEK SGASSFGEKF S RVKFSPSL TLFGGKPMEG WIAVTVSGLV TVSLLKPSGQ VLTSTESLCR LRGRVALADI AFTGGGNIVV ATADGSSASP VQ FYKVCVS VVSEKCRIDT EILPSLFMRC TTDLNRKDKF PAITHLKFLA RDMSEQVLLC ASSQTSSIVE CWSLRKEGLP VNN IFQQIS PVVGDKQPTI LKWRILSATN DLDRVSAVAL PKLPISLTNT DLKVASDTQF YPGLGLALAF HDGSVHIVHR LSLQ TMAVF YSSAAPRPVD EPAMKRPRTA GPAVHLKAMQ LSWTSLALVG IDSHGKLSVL RLSPSMGHPL EVGLALRHLL FLLEY CMVT GYDWWDILLH VQPSMVQSLV EKLHEEYTRQ TAALQQVLST RILAMKASLC KLSPCTVTRV CDYHTKLFLI AISSTL KSL LRPHFLNTPD KSPGDRLTEI CTKITDVDID KVMINLKTEE FVLDMNTLQA LQQLLQWVGD FVLYLLASLP NQGSLLR PG HSFLRDGTSL GMLRELMVVI RIWGLLKPSC LPVYTATSDT QDSMSLLFRL LTKLWICCRD EGPASEPDEA LVDECCLL P SQLLIPSLDW LPASDGLVSR LQPKQPLRLQ FGRAPTLPGS AATLQLDGLA RAPGQPKIDH LRRLHLGACP TEECKACTR CGCVTMLKSP NRTTAVKQWE QRWIKNCLAV EGRGPDACVT SRASEEAPAF VQLGPQSTHH SPRTPRSLDH LHPEDRP

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Macromolecule #13: Mediator of RNA polymerase II transcription subunit 17

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 17
type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 72.984047 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MSGVRAVRIS IESACEKQVH EVGLDGTETY LPPLSMSQNL ARLAQRIDFS QGSGSEEEEA AGTEGDAQEW PGAGSSADQD DEEGVVKFQ PSLWPWDSVR NNLRSALTEM CVLYDVLSIV RDKKFMTLDP VSQDALPPKQ NPQTLQLISK KKSLAGAAQI L LKGAERLT ...String:
MSGVRAVRIS IESACEKQVH EVGLDGTETY LPPLSMSQNL ARLAQRIDFS QGSGSEEEEA AGTEGDAQEW PGAGSSADQD DEEGVVKFQ PSLWPWDSVR NNLRSALTEM CVLYDVLSIV RDKKFMTLDP VSQDALPPKQ NPQTLQLISK KKSLAGAAQI L LKGAERLT KSVTENQENK LQRDFNSELL RLRQHWKLRK VGDKILGDLS YRSAGSLFPH HGTFEVIKNT DLDLDKKIPE DY CPLDVQI PSDLEGSAYI KVSIQKQAPD IGDLGTVNLF KRPLPKSKPG SPHWQTKLEA AQNVLLCKEI FAQLSREAVQ IKS QVPHIV VKNQIISQPF PSLQLSISLC HSSNDKKSQK FATEKQCPED HLYVLEHNLH LLIREFHKQT LSSIMMPHPA SAPF GHKRM RLSGPQAFDK NEINSLQSSE GLLEKIIKQA KHIFLRSRAA ATIDSLASRI EDPQIQAHWS NINDVYESSV KVLIT SQGY EQICKSIQLQ LNIGVEQIRV VHRDGRVITL SYQEQELQDF LLSQMSQHQV HAVQQLAKVM GWQVLSFSNH VGLGPI ESI GNASAITVAS PSGDYAISVR NGPESGSKIM VQFPRNQCKD LPKSDVLQDN KWSHLRGPFK EVQWNKMEGR NFVYKME LL MSALSPCLL

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Macromolecule #14: Mediator of RNA polymerase II transcription subunit 18

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 18
type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 23.69373 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MEAPPVTMMP VTGGTINMME YLLQGSVLDH SLESLIHRLR GLCDNMEPET FLDHEMVFLL KGQQASPFVL RARRSMDRAG APWHLRYLG QPEMGDKNRH ALVRNCVDIA TSENLTDFLM EMGFRMDHEF VAKGHLFRKG IMKIMVYKIF RILVPGNTDS T EALSLSYL ...String:
MEAPPVTMMP VTGGTINMME YLLQGSVLDH SLESLIHRLR GLCDNMEPET FLDHEMVFLL KGQQASPFVL RARRSMDRAG APWHLRYLG QPEMGDKNRH ALVRNCVDIA TSENLTDFLM EMGFRMDHEF VAKGHLFRKG IMKIMVYKIF RILVPGNTDS T EALSLSYL VELSVVAPAG QDMVSDDMKN FAEQLKPLVH LEKIDPKRLM

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Macromolecule #15: Mediator of RNA polymerase II transcription subunit 19

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 19
type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 26.321146 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MENFTALFGA QADPPPPPTA LGFGPGKPPP PPPPPAGGGP GTAPPPTAAT APPGADKSGA GCGPFYLMRE LPGSTELTGS TNLITHYNL EQAYNKFCGK KVKEKLSNFL PDLPGMIDLP GSHDNSSLRS LIEKPPILSS SFNPITGTML AGFRLHTGPL P EQCRLMHI ...String:
MENFTALFGA QADPPPPPTA LGFGPGKPPP PPPPPAGGGP GTAPPPTAAT APPGADKSGA GCGPFYLMRE LPGSTELTGS TNLITHYNL EQAYNKFCGK KVKEKLSNFL PDLPGMIDLP GSHDNSSLRS LIEKPPILSS SFNPITGTML AGFRLHTGPL P EQCRLMHI QPPKKKNKHK HKQSRTQDPV PPETPSDSDH KKKKKKKEED PDRKRKKKEK KKKKNRHSPD HPGMGSSQAS SS SSLR

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Macromolecule #16: Mediator of RNA polymerase II transcription subunit 20

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 20
type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 23.243762 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MGVTCVSQMP VAEGKSVQQT VELLTRKLEM LGAEKQGTFC VDCETYHTAA STLGSQGQTG KLMYVMHNSE YPLSCFALFE NGPCLIADT NFDVLMVKLK GFFQSAKASK IETRGTRYQY CDFLVKVGTV TMGPSARGIS VEVEYGPCVV ASDCWSLLLE F LQSFLGSH ...String:
MGVTCVSQMP VAEGKSVQQT VELLTRKLEM LGAEKQGTFC VDCETYHTAA STLGSQGQTG KLMYVMHNSE YPLSCFALFE NGPCLIADT NFDVLMVKLK GFFQSAKASK IETRGTRYQY CDFLVKVGTV TMGPSARGIS VEVEYGPCVV ASDCWSLLLE F LQSFLGSH TPGAPAVFGN RHDAVYGPAD TMVQYMELFN KIRKQQQVPV AGIR

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Macromolecule #17: Mediator of RNA polymerase II transcription subunit 21

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 21
type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 15.575234 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString:
MADRLTQLQD AVNSLADQFC NAIGVLQQCG PPASFNNIQT AINKDQPANP TEEYAQLFAA LIARTAKDID VLIDSLPSEE STAALQAAS LYKLEEENHE AATCLEDVVY RGDMLLEKIQ SALADIAQSQ LKTRSGTHSQ SLPDS

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Macromolecule #18: Mediator of RNA polymerase II transcription subunit 22

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 22
type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 22.243713 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MAQQRALPQS KETLLQSYNK RLKDDIKSIM DNFTEIIKTA KIEDETQVSR ATQGEQDNYE MHVRAANIVR AGESLMKLVS DLKQFLILN DFPSVNEAID QRNQQLRTLQ EECDRKLITL RDEISIDLYE LEEEYYSSSS SLCEANDLPL CEAYGRLDLD T DSADGLSA ...String:
MAQQRALPQS KETLLQSYNK RLKDDIKSIM DNFTEIIKTA KIEDETQVSR ATQGEQDNYE MHVRAANIVR AGESLMKLVS DLKQFLILN DFPSVNEAID QRNQQLRTLQ EECDRKLITL RDEISIDLYE LEEEYYSSSS SLCEANDLPL CEAYGRLDLD T DSADGLSA PLLASPEPSA GPLQVAAPAH SHAGGPGPTE HA

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Macromolecule #19: Mediator of RNA polymerase II transcription subunit 23

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 23
type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 156.652156 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: METQLQSIFE EVVKTEVIEE AFPGMFMDTP EDEKTKLISC LGAFRQFWGG LSQESHEQCI QWIVKFIHGQ HSPKRISFLY DCLAMAVET GLLPPRLVCE SLINSDTLEW ERTQLWALTF KLVRKIIGGV DYKGVRDLLK VILEKILTIP NTVSSAVVQQ L LAAREVIA ...String:
METQLQSIFE EVVKTEVIEE AFPGMFMDTP EDEKTKLISC LGAFRQFWGG LSQESHEQCI QWIVKFIHGQ HSPKRISFLY DCLAMAVET GLLPPRLVCE SLINSDTLEW ERTQLWALTF KLVRKIIGGV DYKGVRDLLK VILEKILTIP NTVSSAVVQQ L LAAREVIA YILERNACLL PAYFAVTEIR KLYPEGKLPH WLLGNLVSDF VDTFRPTARI NSICGRCSLL PVVNNSGAIC NS WKLDPAT LRFPLKGLLP YDKDLFEPQT ALLRYVLEQP YSRDMVCNML GLNKQHKQRC PVLEDQLVDL VVYAMERSET EEK FDDGGT SQLLWQHLSS QLIFFVLFQF ASFPHMVLSL HQKLAGRGLI KGRDHLMWVL LQFISGSIQK NALADFLPVM KLFD LLYPE KEYIPVPDIN KPQSTHAFAM TCIWIHLNRK AQNDNSKLQI PIPHSLRLHH EFLQQSLRNK SLQMNDYKIA LLCNA YSTN SECFTLPMGA LVETIYGNGI MRIPLPGTNC MASGSITPLP MNLLDSLTVH AKMSLIHSIA TRVIKLAHAK SSVALA PAL VETYSRLLVY MEIESLGIKG FISQLLPTVF KSHAWGILHT LLEMFSYRMH HIQPHYRVQL LSHLHTLAAV AQTNQNQ LH LCVESTALRL ITALGSSEVQ PQFTRFLSDP KTVLSAESEE LNRALILTLA RATHVTDFFT GSDSIQGTWC KDILQTIM S FTPHNWASHT LSCFPGPLQA FFKQNNVPQE SRFNLKKNVE EEYRKWKSMS NENDIITHFS MQGSPPLFLC LLWKMLLET DHINQIGYRV LERIGARALV AHVRTFADFL VYEFSTSAGG QQLNKCIEIL NDMVWKYNIV TLDRLILCLA MRSHEGNEAQ VCYFIIQLL LLKPNDFRNR VSDFVKENSP EHWLQNDWHT KHMNYHKKYP EKLYFEGLAE QVDPPVQIQS PYLPIYFGNV C LRFLPVFD IVIHRFLELL PVSKSLETLL DHLGGLYKFH DRPVTYLYNT LHYYEMHLRD RAFLKRKLVH AIIGSLKDNR PQ GWCLSDT YLKCAMNARE ENPWVPDDTY YCRLIGRLVD TMAGKSPGPF PNCDWRFNEF PNPAAHALHV TCVELMALAV SGK EVGNAL LNVVLKSQPL VPRENITAWM NAIGLIITAL PEPYWIVLHD RIVSVISSPS LTSETEWVGY PFRLFDFTAC HQSY SEMSC SYTLALAHAV WHHSSIGQLS LIPKFLTEVL LPIVKTEFQL LYVYHLVGPF LQRFQQERTR CMIEIGVAFY DMLLN VDQC STHLNYMDPI CDFLYHMKYM FTGDSVKEQV EKIICNLKPA LKLRLRFITH ISKMEPAAVP PQAMNSGSPA PQSNQV PVS LPVTQ

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Macromolecule #20: Mediator of RNA polymerase II transcription subunit 24

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 24
type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 110.430164 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MKVVNLKQAI LQAWKERWSD YQWAINMKKF FPKGATWDIL NLADALLEQA MIGPSPNPLI LSYLKYAISS QMVSYSSVLT AISKFDDFS RDLCVQALLD IMDMFCDRLS CHGKAEECIG LCRALLSALH WLLRCTAASA ERLREGLEAG TPAAGEKQLA M CLQRLEKT ...String:
MKVVNLKQAI LQAWKERWSD YQWAINMKKF FPKGATWDIL NLADALLEQA MIGPSPNPLI LSYLKYAISS QMVSYSSVLT AISKFDDFS RDLCVQALLD IMDMFCDRLS CHGKAEECIG LCRALLSALH WLLRCTAASA ERLREGLEAG TPAAGEKQLA M CLQRLEKT LSSTKNRALL HIAKLEEASS WTAIEHSLLK LGEILANLSN PQLRSQAEQC GTLIRSIPTM LSVHAEQMHK TG FPTVHAV ILLEGTMNLT GETQSLVEQL TMVKRMQHIP TPLFVLEIWK ACFVGLIESP EGTEELKWTA FTFLKIPQVL VKL KKYSHG DKDFTEDVNC AFEFLLKLTP LLDKADQRCN CDCTNFLLQE CGKQGLLSEA SVNNLMAKRK ADREHAPQQK SGEN ANIQP NIQLILRAEP TVTNILKTMD ADHSKSPEGL LGVLGHMLSG KSLDLLLAAA AATGKLKSFA RKFINLNEFT TYGSE ESTK PASVRALLFD ISFLMLCHVA QTYGSEVILS ESRTGAEVPF FETWMQTCMP EEGKILNPDH PCFRPDSTKV ESLVAL LNN SSEMKLVQMK WHEACLSISA AILEILNAWE NGVLAFESIQ KITDNIKGKV CSLAVCAVAW LVAHVRMLGL DEREKSL QM IRQLAGPLFS ENTLQFYNER VVIMNSILER MCADVLQQTA TQIKFPSTGV DTMPYWNLLP PKRPIKEVLT DIFAKVLE K GWVDSRSIHI FDTLLHMGGV YWFCNNLIKE LLKETRKEHT LRAVELLYSI FCLDMQQVTL VLLGHILPGL LTDSSKWHS LMDPPGTALA KLAVWCALSS YSSHKGQAST RQKKRHREDI EDYISLFPLD DVQPSKLMRL LSSNEDDANI LSSPTDRSMS SSLSASQLH TVNMRDPLNR VLANLFLLIS SILGSRTAGP HTQFVQWFME ECVDCLEQGG RGSVLQFMPF TTVSELVKVS A MSSPKVVL AITDLSLPLG RQVAAKAIAA L

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Macromolecule #21: Mediator of RNA polymerase II transcription subunit 26

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 26
type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 65.544438 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MTAAPASPQQ IRDRLLQAID PQSNIRNMVA VLEVISSLEK YPITKEALEE TRLGKLINDV RKKTKNEELA KRAKKLLRSW QKLIEPAHQ HEAALRGLAG ATGSANGGAH NCRPEVGAAG PPRSIHDLKS RNDLQRLPGQ RLDRLGSRKR RGDQRDLGHP G PPPKVSKA ...String:
MTAAPASPQQ IRDRLLQAID PQSNIRNMVA VLEVISSLEK YPITKEALEE TRLGKLINDV RKKTKNEELA KRAKKLLRSW QKLIEPAHQ HEAALRGLAG ATGSANGGAH NCRPEVGAAG PPRSIHDLKS RNDLQRLPGQ RLDRLGSRKR RGDQRDLGHP G PPPKVSKA SHDPLVPNSS PLPTNGISGS PESFASSLDG SGHAGPEGSR LERDENDKHS GKIPVNAVRP HTSSPGLGKP PG PCLQPKA SVLQQLDRVD ETPGPPHPKG PPRCSFSPRN SRHEGSFARQ QSLYAPKGSV PSPSPRPQAL DATQVPSPLP LAQ PSTPPV RRLELLPSAE SPVCWLEQPE SHQRLAGPGC KAGLSPAEPL LSRAGFSPDS SKADSDAASS GGSDSKKKKR YRPR DYTVN LDGQVAEAGV KPVRLKERKL TFDPMTRQIK PLTQKEPVRA DSPVHMEQQS RTELDKQEAK ASLQSPFEQT NWKEL SRNE IIQSYLSRQS SLLSSSGAQT PGAHHFMSEY LKQEESTRQG ARQLHVLVPQ SPPTDLPGLT REVTQDDLDR IQASQW PGV NGCQDTQGNW YDWTQCISLD PHGDDGRLNI LPYVCLD

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Macromolecule #22: Mediator of RNA polymerase II transcription subunit 27

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 27
type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 35.478742 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MADVINVSVN LEAFSQAISA IQALRSSVSR VFDCLKDGMR NKETLEGREK AFIAHFQDNL HSVNRDLNEL ERLSNLVGKP SENHPLHNS GLLSLDPVQD KTPLYSQLLQ AYKWSNKLQY HAGLASGLLN QQSLKRSANQ MGVSAKRRPK AQPTTLVLPP Q YVDDVISR ...String:
MADVINVSVN LEAFSQAISA IQALRSSVSR VFDCLKDGMR NKETLEGREK AFIAHFQDNL HSVNRDLNEL ERLSNLVGKP SENHPLHNS GLLSLDPVQD KTPLYSQLLQ AYKWSNKLQY HAGLASGLLN QQSLKRSANQ MGVSAKRRPK AQPTTLVLPP Q YVDDVISR IDRMFPEMSI HLSRPNGTSA MLLVTLGKVL KVIVVMRSLF IDRTIVKGYN ENVYTEDGKL DIWSKSNYQV FQ KVTDHAT TALLHYQLPQ MPDVVVRSFM TWLRSYIKLF QAPCQRCGKF LQDGLPPTWR DFRTLEAFHD TCRQ

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Macromolecule #23: Mediator of RNA polymerase II transcription subunit 28

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 28
type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 19.540107 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString:
MAAPLGGMFS GQPPGPPQAP PGLPGQASLL QAAPGAPRPS SSTLVDELES SFEACFASLV SQDYVNGTDQ EEIRTGVDQC IQKFLDIAR QTECFFLQKR LQLSVQKPEQ VIKEDVSELR NELQRKDALV QKHLTKLRHW QQVLEDINVQ HKKPADIPQG S LAYLEQAS ANIPAPLKPT

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Macromolecule #24: Mediator of RNA polymerase II transcription subunit 29

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 29
type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 21.089801 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MAASQQQASA ASSAAGVSGP SSAGGPGPQQ QPQPPAQLVG PAQSGLLQQQ QQDFDPVQRY KMLIPQLKES LQTLMKVAAQ NLIQNTNID NGQKSSDGPI QRFDKCLEEF YALCDQLELC LRLAHECLSQ SCDSAKHSPT LVPTATKPDA VQPDSLPYPQ Y LAVIKAQI ...String:
MAASQQQASA ASSAAGVSGP SSAGGPGPQQ QPQPPAQLVG PAQSGLLQQQ QQDFDPVQRY KMLIPQLKES LQTLMKVAAQ NLIQNTNID NGQKSSDGPI QRFDKCLEEF YALCDQLELC LRLAHECLSQ SCDSAKHSPT LVPTATKPDA VQPDSLPYPQ Y LAVIKAQI SCAKDIHTAL LDCANKVTGK TPAPPAGPGG TL

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Macromolecule #25: Mediator of RNA polymerase II transcription subunit 30

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 30
type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 20.301229 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString:
MSTPPLAASG MAPGPFAGPQ AQQAAREVNT ASLCRIGQET VQDIVYRTME IFQLLRNMQL PNGVTYHTGT YQDRLTKLQD NLRQLSVLF RKLRLVYDKC NENCGGMDPI PVEQLIPYVE EDGSKNDDRA GPPRFASEER REIAEVNKKL KQKNQQLKQI M DQLRNLIW DINAMLAMRN

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Macromolecule #26: Mediator of RNA polymerase II transcription subunit 31

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 31
type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 15.826116 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString:
MAAAVAMETD DAGNRLRFQL ELEFVQCLAN PNYLNFLAQR GYFKDKAFVN YLKYLLYWKD PEYAKYLKYP QCLHMLELLQ YEHFRKELV NAQCAKFIDE QQILHWQHYS RKRMRLQQAL AEQQQQNNTS GK

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Macromolecule #27: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 27 / Number of copies: 1 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 8
GridModel: Quantifoil R1.2/1.3 / Material: GOLD / Support film - Material: CARBON / Support film - topology: HOLEY
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 70.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Cs: 2.7 mm
Specialist opticsEnergy filter - Slit width: 20 eV
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: SUPER-RESOLUTION / Average electron dose: 50.0 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Initial angle assignmentType: OTHER
Final angle assignmentType: MAXIMUM LIKELIHOOD
Final reconstructionResolution.type: BY AUTHOR / Resolution: 4.4 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 62871

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Atomic model buiding 1

RefinementSpace: REAL / Protocol: RIGID BODY FIT
Output model

PDB-7enj:
Human Mediator (deletion of MED1-IDR) in a Tail-bent conformation (MED-B)

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