[English] 日本語
Yorodumi
- PDB-7enc: TFIID-based PIC-Mediator holo-complex in fully-assembled state (h... -

+
Open data


ID or keywords:

Loading...

-
Basic information

Entry
Database: PDB / ID: 7enc
TitleTFIID-based PIC-Mediator holo-complex in fully-assembled state (hPIC-MED)
Components
  • (DNA (69-MER)) x 2
  • (DNA-directed RNA ...) x 8
  • (General transcription and DNA repair factor IIH helicase subunit ...) x 2
  • (General transcription factor IIF subunit ...) x 2
  • (General transcription factor IIH subunit ...) x 5
  • (Isoform 2 of Mediator of RNA polymerase II transcription subunit ...) x 2
  • (Mediator of RNA polymerase II transcription subunit ...) x 23
  • (Transcription initiation factor ...) x 16
  • CDK-activating kinase assembly factor MAT1
  • Cyclin-H
  • Cyclin-dependent kinase 7
  • General transcription factor IIE subunit 1
  • RNA_pol_L_2 domain-containing protein
  • RPB10
  • RPB12
  • RPB1POLR2A
  • TATA-box-binding protein
  • TFIIA-a
KeywordsTRANSCRIPTION / mediator / preinitiation complex / transcription initiation
Function / homology
Function and homology information


negative regulation of smooth muscle cell differentiation / cyclin-dependent protein kinase activating kinase holoenzyme complex / MMXD complex / core mediator complex / negative regulation of MHC class I biosynthetic process / spermine transport / core TFIIH complex portion of holo TFIIH complex / positive regulation of DNA helicase activity / central nervous system myelin formation / CAK-ERCC2 complex ...negative regulation of smooth muscle cell differentiation / cyclin-dependent protein kinase activating kinase holoenzyme complex / MMXD complex / core mediator complex / negative regulation of MHC class I biosynthetic process / spermine transport / core TFIIH complex portion of holo TFIIH complex / positive regulation of DNA helicase activity / central nervous system myelin formation / CAK-ERCC2 complex / hair follicle maturation / enucleate erythrocyte development / Cytosolic iron-sulfur cluster assembly / positive regulation of interferon-gamma-mediated signaling pathway / positive regulation of G0 to G1 transition / ventricular system development / mammary gland branching involved in thelarche / TFIIH-class transcription factor complex binding / transcription factor TFIIK complex / retinal pigment epithelium development / negative regulation of histone H4 acetylation / regulation of RNA biosynthetic process / DNA-templated transcription open complex formation / positive regulation of mitotic recombination / pre-snoRNP complex / androgen biosynthetic process / SAGA complex => GO:0000124 / RNA polymerase II core complex assembly / Formation of RNA Pol II elongation complex / Formation of the Early Elongation Complex / Transcriptional regulation by small RNAs / RNA Polymerase II Pre-transcription Events / TP53 Regulates Transcription of DNA Repair Genes / FGFR2 alternative splicing / mRNA Capping / mRNA Splicing - Major Pathway / mRNA Splicing - Minor Pathway / Estrogen-dependent gene expression / Processing of Capped Intron-Containing Pre-mRNA / RNA Polymerase II Promoter Escape / RNA Polymerase II Transcription Pre-Initiation And Promoter Opening / RNA Polymerase II Transcription Initiation / RNA Pol II CTD phosphorylation and interaction with CE / RNA Polymerase II Transcription Elongation / RNA Polymerase II Transcription Initiation And Promoter Clearance / RNA polymerase II transcribes snRNA genes / adult heart development / transcription factor TFTC complex / hair cell differentiation / Gap-filling DNA repair synthesis and ligation in TC-NER / Dual incision in TC-NER / thyroid hormone mediated signaling pathway / Formation of TC-NER Pre-Incision Complex / negative regulation of protein autoubiquitination / nucleotide-excision repair factor 3 complex / negative regulation of RNA polymerase II regulatory region sequence-specific DNA binding / negative regulation of MHC class II biosynthetic process / SAGA complex => GO:0000124 / embryonic cleavage / transcription factor TFIIE complex / embryonic heart tube development / phosphatase activator activity / regulation of vitamin D receptor signaling pathway / TFIIF-class transcription factor complex binding / negative regulation of DNA helicase activity / regulation of cell cycle G1/S phase transition / [RNA-polymerase]-subunit kinase / SLIK (SAGA-like) complex / positive regulation of core promoter binding / transcription factor TFIIH core complex / mRNA transcription by RNA polymerase II / UV protection / transcription factor TFIIH holo complex / regulation of DNA binding / transcription factor TFIIF complex / transcription open complex formation at RNA polymerase II promoter / RNA polymerase I general transcription initiation factor activity / transcriptional start site selection at RNA polymerase II promoter / RNA Polymerase III Transcription Initiation From Type 1 Promoter / RNA Polymerase III Transcription Initiation From Type 2 Promoter / transcription elongation from RNA polymerase I promoter / SAGA complex / G protein-coupled receptor internalization / transcription regulator inhibitor activity / RNA Polymerase III Transcription Initiation From Type 3 Promoter / cyclin-dependent protein serine/threonine kinase activator activity / positive regulation of response to cytokine stimulus / RNA Polymerase III Abortive And Retractive Initiation / inner cell mass cell proliferation / phosphorylation of RNA polymerase II C-terminal domain / RNA polymerase II-specific DNA-binding transcription factor binding / maintenance of protein location in nucleus / thyroid hormone generation / RNA polymerase III general transcription initiation factor activity / histone deubiquitination / transcription factor TFIIA complex / hepatocyte differentiation / Abortive elongation of HIV-1 transcript in the absence of Tat / retinoic acid receptor binding / limb development
Similarity search - Function
Mediator complex subunit 28 / Mediator of RNA polymerase II transcription subunit 26 / Mediator complex subunit 26, middle domain / Mediator complex subunit 26, C-terminal domain / Mediator complex subunit 26 C-terminal / Mediator complex subunit 26 middle domain / Mediator complex subunit 24 N-terminal / Mediator complex, subunit Med24, / Mediator complex subunit 29 / Mediator complex, subunit Med29, metazoa ...Mediator complex subunit 28 / Mediator of RNA polymerase II transcription subunit 26 / Mediator complex subunit 26, middle domain / Mediator complex subunit 26, C-terminal domain / Mediator complex subunit 26 C-terminal / Mediator complex subunit 26 middle domain / Mediator complex subunit 24 N-terminal / Mediator complex, subunit Med24, / Mediator complex subunit 29 / Mediator complex, subunit Med29, metazoa / Mediator of RNA polymerase II transcription subunit 15 / Mediator complex, subunit Med23 / ARC105 or Med15 subunit of Mediator complex non-fungal / Mediator complex subunit 23 / Mediator complex, subunit Med16 / Mediator complex subunit 16 / Mediator of RNA pol II transcription subunit 19 / Mediator complex, subunit Med6, metazoa/plant / Mediator complex subunit 30 / Mediator complex, subunit Med28 / Mediator complex, subunit Med30, metazoa / Mediator complex, subunit Med19, metazoa / Mediator of RNA polymerase II transcription subunit 9, metazoan / Mediator complex, subunit Med27 / Transcription factor subunit Med10 of Mediator complex / Mediator complex subunit 27 / Mediator complex, subunit Med10 / RNA polymerase II transcription mediator complex subunit 9 / Mediator of RNA polymerase II transcription subunit 9 / Vitamin-D-receptor interacting Mediator subunit 4 / Mediator complex, subunit Med4 / Mediator complex subunit MED14 / Mediator complex, subunit Med14 / SOH1 / Mediator complex, subunit Med31 / Mediator of RNA polymerase II, subunit Med31 superfamily / Mediator complex, subunit Med21 / Subunit 21 of Mediator complex / Mediator complex protein / Mediator complex, subunit Med11 / CyclinH/Ccl1 / MED7 protein / Mediator complex, subunit Med7/Med21-like / Cyclin-dependent kinase 7 / Mediator complex, subunit Med7 / Cyclin, C-terminal domain 2 / Cyclin C-terminal domain / Mediator complex, subunit Med20 / TATA-binding related factor (TRF) of subunit 20 of Mediator complex / Subunit 17 of Mediator complex / Mediator complex, subunit Med6 superfamily / MED6 mediator sub complex component / Mediator complex, subunit Med6 / Mediator complex, subunit Med17 / Soluble quinoprotein glucose/sorbosone dehydrogenase / Mediator complex, subunit Med8, fungi/metazoa / Mediator of RNA polymerase II transcription subunit 22 / Surfeit locus protein 5 subunit 22 of Mediator complex / Mediator of RNA polymerase II transcription complex subunit 8 / : / Med18 protein / Mediator complex, subunit Med18 / Domain in the N-terminus of transcription elongation factor S-II (and elsewhere) / Transcription elongation factor, TFIIS/CRSP70, N-terminal, sub-type / TAFII28-like protein domain / hTAFII28-like protein conserved region / TAFII55 protein conserved region / Transcription initiation factor TFIID subunit 7 / TAFII55 protein, conserved region / TAFII55 protein conserved region / TFIIS helical bundle-like domain / Transcription initiation factor TFIID subunit 2 / Transcription factor IIS, N-terminal / TFIIS N-terminal domain profile. / Transcription initiation factor TFIID subunit 8 / Transcription factor TFIID complex subunit 8 C-term / Transcription factor TFIID, subunit 8, C-terminal / Transcription initiation factor TFIID component TAF4 family / Transcription initiation factor IID, 18kD subunit / Transcription initiation factor IID, subunit 13 / Transcription initiation factor TFIID component TAF4, C-terminal / TAFH/NHR1 domain superfamily / Bromodomain associated / TAFH/NHR1 domain profile. / TAF homology / NHR1 homology to TAF / Bromodomain transcription factors and PHD domain containing proteins / Bromodomain associated domain / Transcription initiation factor IID, 31kD subunit / TAFH/NHR1 / Transcription initiation factor TFIID subunit 12 / Transcription initiation factor TFIID subunit 5 / CDK-activating kinase assembly factor MAT1 / Cdk-activating kinase assembly factor MAT1, centre / Transcription initiation factor TFIID subunit A / Cdk-activating kinase assembly factor MAT1/Tfb3 / Zinc finger, C3HC4 type (RING finger) / TFIID subunit TAF5, NTD2 domain superfamily / C-terminal general transcription factor TFIIE alpha / WD40 associated region in TFIID subunit, NTD2 domain
Similarity search - Domain/homology
Transcription initiation factor TFIID subunit 2 / Transcription initiation factor TFIID subunit 3 / Transcription initiation factor TFIID subunit 9 / Transcription initiation factor TFIID subunit 12 / Mediator of RNA polymerase II transcription subunit 1 / Transcription initiation factor TFIID subunit 7 / Transcription initiation factor TFIID subunit 13 / Transcription initiation factor TFIID subunit 11 / Transcription initiation factor TFIID subunit 5 / Mediator of RNA polymerase II transcription subunit 22 ...Transcription initiation factor TFIID subunit 2 / Transcription initiation factor TFIID subunit 3 / Transcription initiation factor TFIID subunit 9 / Transcription initiation factor TFIID subunit 12 / Mediator of RNA polymerase II transcription subunit 1 / Transcription initiation factor TFIID subunit 7 / Transcription initiation factor TFIID subunit 13 / Transcription initiation factor TFIID subunit 11 / Transcription initiation factor TFIID subunit 5 / Mediator of RNA polymerase II transcription subunit 22 / Mediator of RNA polymerase II transcription subunit 21 / General transcription factor IIH subunit 2 / General transcription factor IIH subunit 5 / General transcription factor IIH subunit 3 / Mediator of RNA polymerase II transcription subunit 27 / Mediator of RNA polymerase II transcription subunit 4 / Transcription initiation factor TFIID subunit 8 / Mediator of RNA polymerase II transcription subunit 17 / Transcription initiation factor IIB / Mediator of RNA polymerase II transcription subunit 16 / Mediator of RNA polymerase II transcription subunit 23 / Mediator of RNA polymerase II transcription subunit 11 / Mediator of RNA polymerase II transcription subunit 29 / Mediator of RNA polymerase II transcription subunit 9 / Mediator of RNA polymerase II transcription subunit 20 / General transcription factor IIH subunit 4 / Mediator of RNA polymerase II transcription subunit 28 / Mediator of RNA polymerase II transcription subunit 18 / Mediator of RNA polymerase II transcription subunit 10 / Mediator of RNA polymerase II transcription subunit 15 / Mediator of RNA polymerase II transcription subunit 30 / Mediator of RNA polymerase II transcription subunit 8 / Transcription initiation factor TFIID subunit 10 / IRON/SULFUR CLUSTER / DNA-directed RNA polymerase II subunit RPB9 / Mediator of RNA polymerase II transcription subunit 14 / DNA / DNA (> 10) / DNA-directed RNA polymerase II subunit RPB4 / Uncharacterized protein / DNA-directed RNA polymerase subunit beta / RNA_pol_L_2 domain-containing protein / DNA-directed RNA polymerases I, II, and III subunit RPABC3 / DNA-directed RNA polymerase II subunit F / DNA-directed RNA polymerase II subunit RPB7 / DNA-directed RNA polymerase II subunit E / Mediator of RNA polymerase II transcription subunit 19 / DNA-directed RNA polymerase II subunit RPB3 / Transcription initiation factor TFIID subunit 4 / Mediator of RNA polymerase II transcription subunit 7 / Mediator of RNA polymerase II transcription subunit 24 / Transcription initiation factor IIA subunit 2 / Transcription initiation factor IIE subunit beta / CDK-activating kinase assembly factor MAT1 / Cyclin-H / Cyclin-dependent kinase 7 / Transcription initiation factor TFIID subunit 6 / General transcription factor IIF subunit 1 / General transcription factor IIH subunit 1 / General transcription factor IIE subunit 1 / Mediator of RNA polymerase II transcription subunit 6 / Transcription initiation factor TFIID subunit 1 / TATA-box-binding protein / General transcription and DNA repair factor IIH helicase subunit XPB / General transcription and DNA repair factor IIH helicase subunit XPD / General transcription factor IIF subunit 2 / Mediator of RNA polymerase II transcription subunit 26 / Mediator of RNA polymerase II transcription subunit 31
Similarity search - Component
Biological speciesHomo sapiens (human)
Sus scrofa (pig)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 4.13 Å
AuthorsChen, X. / Qi, Y. / Wang, X. / Wu, Z. / Yin, X. / Li, J. / Liu, W. / Xu, Y.
CitationJournal: Science / Year: 2021
Title: Structures of the human Mediator and Mediator-bound preinitiation complex.
Authors: Xizi Chen / Xiaotong Yin / Jiabei Li / Zihan Wu / Yilun Qi / Xinxin Wang / Weida Liu / Yanhui Xu /
Abstract: The 1.3-megadalton transcription factor IID (TFIID) is required for preinitiation complex (PIC) assembly and RNA polymerase II (Pol II)-mediated transcription initiation on almost all genes. The 26- ...The 1.3-megadalton transcription factor IID (TFIID) is required for preinitiation complex (PIC) assembly and RNA polymerase II (Pol II)-mediated transcription initiation on almost all genes. The 26-subunit Mediator stimulates transcription and cyclin-dependent kinase 7 (CDK7)-mediated phosphorylation of the Pol II C-terminal domain (CTD). We determined the structures of human Mediator in the Tail module-extended (at near-atomic resolution) and Tail-bent conformations and structures of TFIID-based PIC-Mediator (76 polypeptides, ~4.1 megadaltons) in four distinct conformations. PIC-Mediator assembly induces concerted reorganization (Head-tilting and Middle-down) of Mediator and creates a Head-Middle sandwich, which stabilizes two CTD segments and brings CTD to CDK7 for phosphorylation; this suggests a CTD-gating mechanism favorable for phosphorylation. The TFIID-based PIC architecture modulates Mediator organization and TFIIH stabilization, underscoring the importance of TFIID in orchestrating PIC-Mediator assembly.
History
DepositionApr 16, 2021Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0May 26, 2021Provider: repository / Type: Initial release
Revision 1.1Jun 16, 2021Group: Database references / Category: citation / Item: _citation.journal_volume

-
Structure visualization

Movie
  • Deposited structure unit
  • Imaged by Jmol
  • Download
  • Simplified surface model + fitted atomic model
  • EMDB-31207
  • Imaged by Jmol
  • Download
  • Superimposition on EM map
  • EMDB-31207
  • Imaged by UCSF Chimera
  • Download
Movie viewer
Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

-
Assembly

Deposited unit
a: Mediator of RNA polymerase II transcription subunit 1
m: Mediator of RNA polymerase II transcription subunit 31
d: Mediator of RNA polymerase II transcription subunit 4
f: Mediator of RNA polymerase II transcription subunit 6
g: Mediator of RNA polymerase II transcription subunit 7
h: Isoform 2 of Mediator of RNA polymerase II transcription subunit 8
r: Mediator of RNA polymerase II transcription subunit 18
t: Mediator of RNA polymerase II transcription subunit 20
j: Mediator of RNA polymerase II transcription subunit 10
k: Mediator of RNA polymerase II transcription subunit 11
n: Mediator of RNA polymerase II transcription subunit 14
q: Mediator of RNA polymerase II transcription subunit 17
s: Mediator of RNA polymerase II transcription subunit 19
u: Mediator of RNA polymerase II transcription subunit 21
v: Mediator of RNA polymerase II transcription subunit 22
z: Mediator of RNA polymerase II transcription subunit 26
c: Mediator of RNA polymerase II transcription subunit 27
e: Mediator of RNA polymerase II transcription subunit 28
b: Mediator of RNA polymerase II transcription subunit 29
l: Mediator of RNA polymerase II transcription subunit 30
o: Mediator of RNA polymerase II transcription subunit 15
i: Mediator of RNA polymerase II transcription subunit 9
0: CDK-activating kinase assembly factor MAT1
8: Cyclin-dependent kinase 7
9: Cyclin-H
1: General transcription factor IIH subunit 1
2: General transcription factor IIH subunit 2
3: General transcription factor IIH subunit 3
4: General transcription factor IIH subunit 4
5: General transcription factor IIH subunit 5
6: General transcription and DNA repair factor IIH helicase subunit XPB
7: General transcription and DNA repair factor IIH helicase subunit XPD
EA: General transcription factor IIE subunit 1
EB: Transcription initiation factor IIE subunit beta
DA: Transcription initiation factor TFIID subunit 1
DB: Transcription initiation factor TFIID subunit 2
DD: Transcription initiation factor TFIID subunit 4
DE: Transcription initiation factor TFIID subunit 5
DF: Transcription initiation factor TFIID subunit 6
DG: Transcription initiation factor TFIID subunit 7
DH: Transcription initiation factor TFIID subunit 8
DI: Transcription initiation factor TFIID subunit 9
DJ: Transcription initiation factor TFIID subunit 10
DL: Transcription initiation factor TFIID subunit 12
Dc: Transcription initiation factor TFIID subunit 3
Dd: Transcription initiation factor TFIID subunit 4
De: Transcription initiation factor TFIID subunit 5
Df: Transcription initiation factor TFIID subunit 6
Di: Transcription initiation factor TFIID subunit 9
Dj: Transcription initiation factor TFIID subunit 10
Dk: Transcription initiation factor TFIID subunit 11
Dl: Transcription initiation factor TFIID subunit 12
Dm: Transcription initiation factor TFIID subunit 13
DO: Transcription initiation factor IIA subunit 2
DP: TATA-box-binding protein
DQ: TFIIA-a
BA: Transcription initiation factor IIB
FB: General transcription factor IIF subunit 2
X: DNA (69-MER)
Y: DNA (69-MER)
PA: RPB1
PB: DNA-directed RNA polymerase subunit beta
PC: DNA-directed RNA polymerase II subunit RPB3
PD: DNA-directed RNA polymerase II subunit RPB4
PE: DNA-directed RNA polymerase II subunit E
PF: DNA-directed RNA polymerase II subunit F
PG: DNA-directed RNA polymerase II subunit RPB7
PH: DNA-directed RNA polymerases I, II, and III subunit RPABC3
PI: DNA-directed RNA polymerase II subunit RPB9
PJ: RPB10
PK: RNA_pol_L_2 domain-containing protein
PL: RPB12
FA: General transcription factor IIF subunit 1
w: Mediator of RNA polymerase II transcription subunit 23
x: Mediator of RNA polymerase II transcription subunit 24
p: Isoform 2 of Mediator of RNA polymerase II transcription subunit 16
hetero molecules


Theoretical massNumber of molelcules
Total (without water)3,882,03794
Polymers3,880,61476
Non-polymers1,42218
Water0
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_5551

-
Components

+
Mediator of RNA polymerase II transcription subunit ... , 23 types, 23 molecules amdfgrtjknqsuvzcebloiwx

#1: Protein Mediator of RNA polymerase II transcription subunit 1


Mass: 168697.328 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: MED1 / Production host: Homo sapiens (human) / References: UniProt: Q15648
#2: Protein Mediator of RNA polymerase II transcription subunit 31


Mass: 15826.116 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: MED31 / Production host: Homo sapiens (human) / References: UniProt: Q9Y3C7
#3: Protein Mediator of RNA polymerase II transcription subunit 4


Mass: 29781.424 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: MED4 / Production host: Homo sapiens (human) / References: UniProt: Q9NPJ6
#4: Protein Mediator of RNA polymerase II transcription subunit 6


Mass: 28464.627 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: MED6 / Production host: Homo sapiens (human) / References: UniProt: O75586
#5: Protein Mediator of RNA polymerase II transcription subunit 7


Mass: 27259.244 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: MED7 / Production host: Homo sapiens (human) / References: UniProt: O43513
#7: Protein Mediator of RNA polymerase II transcription subunit 18


Mass: 23693.730 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: MED18 / Production host: Homo sapiens (human) / References: UniProt: Q9BUE0
#8: Protein Mediator of RNA polymerase II transcription subunit 20


Mass: 23243.762 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: MED20 / Production host: Homo sapiens (human) / References: UniProt: Q9H944
#9: Protein Mediator of RNA polymerase II transcription subunit 10


Mass: 15710.969 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: MED10 / Production host: Homo sapiens (human) / References: UniProt: Q9BTT4
#10: Protein Mediator of RNA polymerase II transcription subunit 11


Mass: 13145.829 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: MED11 / Production host: Homo sapiens (human) / References: UniProt: Q9P086
#11: Protein Mediator of RNA polymerase II transcription subunit 14


Mass: 160829.672 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: MED14 / Production host: Homo sapiens (human) / References: UniProt: O60244
#12: Protein Mediator of RNA polymerase II transcription subunit 17


Mass: 72984.047 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: MED17 / Production host: Homo sapiens (human) / References: UniProt: Q9NVC6
#13: Protein Mediator of RNA polymerase II transcription subunit 19


Mass: 26321.146 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: MED19 / Production host: Homo sapiens (human) / References: UniProt: A0JLT2
#14: Protein Mediator of RNA polymerase II transcription subunit 21


Mass: 15575.234 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: MED21 / Production host: Homo sapiens (human) / References: UniProt: Q13503
#15: Protein Mediator of RNA polymerase II transcription subunit 22


Mass: 22243.713 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: MED22 / Production host: Homo sapiens (human) / References: UniProt: Q15528
#16: Protein Mediator of RNA polymerase II transcription subunit 26


Mass: 65544.438 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: MED26 / Production host: Homo sapiens (human) / References: UniProt: O95402
#17: Protein Mediator of RNA polymerase II transcription subunit 27


Mass: 35478.742 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: MED27 / Production host: Homo sapiens (human) / References: UniProt: Q6P2C8
#18: Protein Mediator of RNA polymerase II transcription subunit 28


Mass: 19540.107 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: MED28 / Production host: Homo sapiens (human) / References: UniProt: Q9H204
#19: Protein Mediator of RNA polymerase II transcription subunit 29


Mass: 21089.801 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: MED29 / Production host: Homo sapiens (human) / References: UniProt: Q9NX70
#20: Protein Mediator of RNA polymerase II transcription subunit 30


Mass: 20301.229 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: MED30 / Production host: Homo sapiens (human) / References: UniProt: Q96HR3
#21: Protein Mediator of RNA polymerase II transcription subunit 15


Mass: 86830.102 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: MED15 / Production host: Homo sapiens (human) / References: UniProt: Q96RN5
#22: Protein Mediator of RNA polymerase II transcription subunit 9


Mass: 16422.750 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: MED9 / Production host: Homo sapiens (human) / References: UniProt: Q9NWA0
#68: Protein Mediator of RNA polymerase II transcription subunit 23


Mass: 156652.156 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: MED23 / Production host: Homo sapiens (human) / References: UniProt: Q9ULK4
#69: Protein Mediator of RNA polymerase II transcription subunit 24


Mass: 110430.164 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: MED24 / Production host: Homo sapiens (human) / References: UniProt: O75448

+
Isoform 2 of Mediator of RNA polymerase II transcription subunit ... , 2 types, 2 molecules hp

#6: Protein Isoform 2 of Mediator of RNA polymerase II transcription subunit 8


Mass: 29112.906 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: MED8 / Production host: Homo sapiens (human) / References: UniProt: Q96G25
#70: Protein Isoform 2 of Mediator of RNA polymerase II transcription subunit 16


Mass: 93136.039 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: MED16 / Production host: Homo sapiens (human) / References: UniProt: Q9Y2X0

+
Protein , 10 types, 10 molecules 089EADPDQPAPJPKPL

#23: Protein CDK-activating kinase assembly factor MAT1 / CDK7/cyclin-H assembly factor / Cyclin-G1-interacting protein / Menage a trois / RING finger ...CDK7/cyclin-H assembly factor / Cyclin-G1-interacting protein / Menage a trois / RING finger protein 66 / RING finger protein MAT1 / p35 / p36


Mass: 35873.965 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: MNAT1, CAP35, MAT1, RNF66 / Production host: Homo sapiens (human) / References: UniProt: P51948
#24: Protein Cyclin-dependent kinase 7 / / 39 kDa protein kinase / p39 Mo15 / CDK-activating kinase 1 / Cell division protein kinase 7 / ...39 kDa protein kinase / p39 Mo15 / CDK-activating kinase 1 / Cell division protein kinase 7 / Serine/threonine-protein kinase 1 / TFIIH basal transcription factor complex kinase subunit


Mass: 39090.344 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: CDK7, CAK, CAK1, CDKN7, MO15, STK1 / Production host: Homo sapiens (human)
References: UniProt: P50613, cyclin-dependent kinase, [RNA-polymerase]-subunit kinase
#25: Protein Cyclin-H / MO15-associated protein / p34 / p37


Mass: 37695.473 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: CCNH / Production host: Homo sapiens (human) / References: UniProt: P51946
#33: Protein General transcription factor IIE subunit 1 / General transcription factor IIE 56 kDa subunit / Transcription initiation factor IIE subunit alpha ...General transcription factor IIE 56 kDa subunit / Transcription initiation factor IIE subunit alpha / TFIIE-alpha


Mass: 49516.094 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: GTF2E1, TF2E1 / Production host: Homo sapiens (human) / References: UniProt: P29083
#49: Protein TATA-box-binding protein / TATA sequence-binding protein / TATA-binding factor / TATA-box factor / Transcription initiation ...TATA sequence-binding protein / TATA-binding factor / TATA-box factor / Transcription initiation factor TFIID TBP subunit


Mass: 37729.938 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: TBP, GTF2D1, TF2D, TFIID / Production host: Homo sapiens (human) / References: UniProt: P20226
#50: Protein TFIIA-a


Mass: 34199.234 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Production host: Homo sapiens (human)
#55: Protein RPB1 / POLR2A


Mass: 217393.016 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Sus scrofa (pig)
#64: Protein RPB10


Mass: 7655.123 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Sus scrofa (pig)
#65: Protein RNA_pol_L_2 domain-containing protein


Mass: 13310.284 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Sus scrofa (pig) / References: UniProt: A0A4X1UK25
#66: Protein RPB12


Mass: 7018.244 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Sus scrofa (pig) / References: UniProt: A0A4X1TRS6

+
General transcription factor IIH subunit ... , 5 types, 5 molecules 12345

#26: Protein General transcription factor IIH subunit 1 / Basic transcription factor 2 62 kDa subunit / BTF2 p62 / General transcription factor IIH ...Basic transcription factor 2 62 kDa subunit / BTF2 p62 / General transcription factor IIH polypeptide 1 / TFIIH basal transcription factor complex p62 subunit


Mass: 62116.492 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: GTF2H1, BTF2 / Production host: Homo sapiens (human) / References: UniProt: P32780
#27: Protein General transcription factor IIH subunit 2 / Basic transcription factor 2 44 kDa subunit / BTF2 p44 / General transcription factor IIH ...Basic transcription factor 2 44 kDa subunit / BTF2 p44 / General transcription factor IIH polypeptide 2 / TFIIH basal transcription factor complex p44 subunit


Mass: 44481.996 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: GTF2H2, BTF2P44 / Production host: Homo sapiens (human) / References: UniProt: Q13888
#28: Protein General transcription factor IIH subunit 3 / Basic transcription factor 2 34 kDa subunit / BTF2 p34 / General transcription factor IIH ...Basic transcription factor 2 34 kDa subunit / BTF2 p34 / General transcription factor IIH polypeptide 3 / TFIIH basal transcription factor complex p34 subunit


Mass: 34416.008 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: GTF2H3 / Production host: Homo sapiens (human) / References: UniProt: Q13889
#29: Protein General transcription factor IIH subunit 4 / Basic transcription factor 2 52 kDa subunit / BTF2 p52 / General transcription factor IIH ...Basic transcription factor 2 52 kDa subunit / BTF2 p52 / General transcription factor IIH polypeptide 4 / TFIIH basal transcription factor complex p52 subunit


Mass: 52245.156 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: GTF2H4 / Production host: Homo sapiens (human) / References: UniProt: Q92759
#30: Protein General transcription factor IIH subunit 5 / General transcription factor IIH polypeptide 5 / TFB5 ortholog / TFIIH basal transcription factor ...General transcription factor IIH polypeptide 5 / TFB5 ortholog / TFIIH basal transcription factor complex TTD-A subunit


Mass: 8060.362 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: GTF2H5, C6orf175, TTDA / Production host: Homo sapiens (human) / References: UniProt: Q6ZYL4

+
General transcription and DNA repair factor IIH helicase subunit ... , 2 types, 2 molecules 67

#31: Protein General transcription and DNA repair factor IIH helicase subunit XPB / TFIIH subunit XPB / Basic transcription factor 2 89 kDa subunit / BTF2 p89 / DNA excision repair ...TFIIH subunit XPB / Basic transcription factor 2 89 kDa subunit / BTF2 p89 / DNA excision repair protein ERCC-3 / DNA repair protein complementing XP-B cells / TFIIH basal transcription factor complex 89 kDa subunit / TFIIH 89 kDa subunit / TFIIH p89 / Xeroderma pigmentosum group B-complementing protein


Mass: 89404.734 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: ERCC3, XPB, XPBC / Production host: Homo sapiens (human) / References: UniProt: P19447, DNA helicase
#32: Protein General transcription and DNA repair factor IIH helicase subunit XPD / TFIIH subunit XPD / Basic transcription factor 2 80 kDa subunit / BTF2 p80 / CXPD / DNA excision ...TFIIH subunit XPD / Basic transcription factor 2 80 kDa subunit / BTF2 p80 / CXPD / DNA excision repair protein ERCC-2 / DNA repair protein complementing XP-D cells / TFIIH basal transcription factor complex 80 kDa subunit / TFIIH 80 kDa subunit / TFIIH p80 / Xeroderma pigmentosum group D-complementing protein


Mass: 87021.078 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: ERCC2, XPD, XPDC / Production host: Homo sapiens (human) / References: UniProt: P18074, DNA helicase

+
Transcription initiation factor ... , 16 types, 22 molecules EBDADBDDDdDEDeDFDfDGDHDIDiDJDjDLDlDcDkDmDOBA

#34: Protein Transcription initiation factor IIE subunit beta / TFIIE-beta / General transcription factor IIE subunit 2


Mass: 33106.824 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: GTF2E2, TF2E2 / Production host: Homo sapiens (human) / References: UniProt: P29084
#35: Protein Transcription initiation factor TFIID subunit 1 / Cell cycle gene 1 protein / TBP-associated factor 250 kDa / p250 / Transcription initiation factor ...Cell cycle gene 1 protein / TBP-associated factor 250 kDa / p250 / Transcription initiation factor TFIID 250 kDa subunit / TAF(II)250 / TAFII-250 / TAFII250


Mass: 212956.172 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: TAF1, BA2R, CCG1, CCGS, TAF2A / Production host: Homo sapiens (human)
References: UniProt: P21675, histone acetyltransferase, non-specific serine/threonine protein kinase
#36: Protein Transcription initiation factor TFIID subunit 2 / 150 kDa cofactor of initiator function / RNA polymerase II TBP-associated factor subunit B / TBP- ...150 kDa cofactor of initiator function / RNA polymerase II TBP-associated factor subunit B / TBP-associated factor 150 kDa / Transcription initiation factor TFIID 150 kDa subunit / TAF(II)150 / TAFII-150 / TAFII150


Mass: 137159.984 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: TAF2, CIF150, TAF2B / Production host: Homo sapiens (human) / References: UniProt: Q6P1X5
#37: Protein Transcription initiation factor TFIID subunit 4 / RNA polymerase II TBP-associated factor subunit C / TBP-associated factor 4 / Transcription ...RNA polymerase II TBP-associated factor subunit C / TBP-associated factor 4 / Transcription initiation factor TFIID 130 kDa subunit / TAF(II)130 / TAFII-130 / TAFII130 / Transcription initiation factor TFIID 135 kDa subunit / TAF(II)135 / TAFII-135 / TAFII135


Mass: 110221.883 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: TAF4, TAF2C, TAF2C1, TAF4A, TAFII130, TAFII135 / Production host: Homo sapiens (human) / References: UniProt: O00268
#38: Protein Transcription initiation factor TFIID subunit 5 / Transcription initiation factor TFIID 100 kDa subunit / TAF(II)100 / TAFII-100 / TAFII100


Mass: 86932.109 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: TAF5, TAF2D / Production host: Homo sapiens (human) / References: UniProt: Q15542
#39: Protein Transcription initiation factor TFIID subunit 6 / RNA polymerase II TBP-associated factor subunit E / Transcription initiation factor TFIID 70 kDa ...RNA polymerase II TBP-associated factor subunit E / Transcription initiation factor TFIID 70 kDa subunit / TAF(II)70 / TAFII-70 / TAFII70 / Transcription initiation factor TFIID 80 kDa subunit / TAF(II)80 / TAFII-80 / TAFII80


Mass: 72749.297 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: TAF6, TAF2E, TAFII70 / Production host: Homo sapiens (human) / References: UniProt: P49848
#40: Protein Transcription initiation factor TFIID subunit 7 / RNA polymerase II TBP-associated factor subunit F / Transcription initiation factor TFIID 55 kDa ...RNA polymerase II TBP-associated factor subunit F / Transcription initiation factor TFIID 55 kDa subunit / TAF(II)55 / TAFII-55 / TAFII55


Mass: 40325.117 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: TAF7, TAF2F, TAFII55 / Production host: Homo sapiens (human) / References: UniProt: Q15545
#41: Protein Transcription initiation factor TFIID subunit 8 / Protein taube nuss / TBP-associated factor 43 kDa / TBP-associated factor 8 / Transcription ...Protein taube nuss / TBP-associated factor 43 kDa / TBP-associated factor 8 / Transcription initiation factor TFIID 43 kDa subunit / TAFII-43 / TAFII43 / hTAFII43


Mass: 34304.359 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: TAF8, TAFII43, TBN / Production host: Homo sapiens (human) / References: UniProt: Q7Z7C8
#42: Protein Transcription initiation factor TFIID subunit 9 / RNA polymerase II TBP-associated factor subunit G / STAF31/32 / Transcription initiation factor ...RNA polymerase II TBP-associated factor subunit G / STAF31/32 / Transcription initiation factor TFIID 31 kDa subunit / TAFII-31 / TAFII31 / Transcription initiation factor TFIID 32 kDa subunit / TAFII-32 / TAFII32


Mass: 29006.838 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: TAF9, TAF2G, TAFII31 / Production host: Homo sapiens (human) / References: UniProt: Q16594
#43: Protein Transcription initiation factor TFIID subunit 10 / STAF28 / Transcription initiation factor TFIID 30 kDa subunit / TAF(II)30 / TAFII-30 / TAFII30


Mass: 21731.248 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: TAF10, TAF2A, TAF2H, TAFII30 / Production host: Homo sapiens (human) / References: UniProt: Q12962
#44: Protein Transcription initiation factor TFIID subunit 12 / Transcription initiation factor TFIID 20/15 kDa subunits / TAFII-20/TAFII-15 / TAFII20/TAFII15


Mass: 17948.467 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: TAF12, TAF15, TAF2J, TAFII20 / Production host: Homo sapiens (human) / References: UniProt: Q16514
#45: Protein Transcription initiation factor TFIID subunit 3 / 140 kDa TATA box-binding protein-associated factor / TBP-associated factor 3 / Transcription ...140 kDa TATA box-binding protein-associated factor / TBP-associated factor 3 / Transcription initiation factor TFIID 140 kDa subunit / TAF(II)140 / TAF140 / TAFII-140 / TAFII140


Mass: 103769.320 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: TAF3 / Production host: Homo sapiens (human) / References: UniProt: Q5VWG9
#46: Protein Transcription initiation factor TFIID subunit 11 / TFIID subunit p30-beta / Transcription initiation factor TFIID 28 kDa subunit / TAF(II)28 / TAFII-28 / TAFII28


Mass: 23340.094 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: TAF11, TAF2I, PRO2134 / Production host: Homo sapiens (human) / References: UniProt: Q15544
#47: Protein Transcription initiation factor TFIID subunit 13 / Transcription initiation factor TFIID 18 kDa subunit / TAF(II)18 / TAFII-18 / TAFII18


Mass: 14307.068 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: TAF13, TAF2K, TAFII18 / Production host: Homo sapiens (human) / References: UniProt: Q15543
#48: Protein Transcription initiation factor IIA subunit 2 / General transcription factor IIA subunit 2 / TFIIA p12 subunit / TFIIA-12 / TFIIAS / Transcription ...General transcription factor IIA subunit 2 / TFIIA p12 subunit / TFIIA-12 / TFIIAS / Transcription initiation factor IIA gamma chain / TFIIA-gamma


Mass: 12469.091 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: GTF2A2, TF2A2 / Production host: Homo sapiens (human) / References: UniProt: P52657
#51: Protein Transcription initiation factor IIB / General transcription factor TFIIB / S300-II


Mass: 34877.949 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: GTF2B, TF2B, TFIIB / Production host: Homo sapiens (human) / References: UniProt: Q00403, histone acetyltransferase

+
General transcription factor IIF subunit ... , 2 types, 2 molecules FBFA

#52: Protein General transcription factor IIF subunit 2 / ATP-dependent helicase GTF2F2 / General transcription factor IIF 30 kDa subunit / Transcription ...ATP-dependent helicase GTF2F2 / General transcription factor IIF 30 kDa subunit / Transcription initiation factor IIF subunit beta / TFIIF-beta / Transcription initiation factor RAP30


Mass: 28427.309 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: GTF2F2, RAP30 / Production host: Homo sapiens (human) / References: UniProt: P13984, DNA helicase
#67: Protein General transcription factor IIF subunit 1 / General transcription factor IIF 74 kDa subunit / Transcription initiation factor IIF subunit alpha ...General transcription factor IIF 74 kDa subunit / Transcription initiation factor IIF subunit alpha / TFIIF-alpha / Transcription initiation factor RAP74


Mass: 58343.578 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: GTF2F1, RAP74 / Production host: Homo sapiens (human) / References: UniProt: P35269

+
DNA chain , 2 types, 2 molecules XY

#53: DNA chain DNA (69-MER)


Mass: 21459.699 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) Homo sapiens (human)
#54: DNA chain DNA (69-MER)


Mass: 21094.428 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) Homo sapiens (human)

+
DNA-directed RNA ... , 8 types, 8 molecules PBPCPDPEPFPGPHPI

#56: Protein DNA-directed RNA polymerase subunit beta / Polymerase


Mass: 134041.422 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Sus scrofa (pig)
References: UniProt: A0A4X1TVZ5, DNA-directed RNA polymerase
#57: Protein DNA-directed RNA polymerase II subunit RPB3 / Polymerase / RNA polymerase II subunit C


Mass: 31439.074 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Sus scrofa (pig) / References: UniProt: I3LCH3
#58: Protein DNA-directed RNA polymerase II subunit RPB4 / Polymerase / DNA-directed RNA polymerase II subunit RPB4 isoform X1


Mass: 16331.255 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Sus scrofa (pig) / References: UniProt: A0A481BYI6
#59: Protein DNA-directed RNA polymerase II subunit E / Polymerase / RPB5 homolog


Mass: 24644.318 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Sus scrofa (pig) / References: UniProt: A0A4X1VTX4
#60: Protein DNA-directed RNA polymerase II subunit F / Polymerase / DNA-directed RNA polymerases I / II / and III subunit RPABC2 / RPB6 homolog


Mass: 14477.001 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Sus scrofa (pig) / References: UniProt: A0A4X1VEK9
#61: Protein DNA-directed RNA polymerase II subunit RPB7 / Polymerase


Mass: 19314.283 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Sus scrofa (pig) / References: UniProt: A0A4X1VKG7
#62: Protein DNA-directed RNA polymerases I, II, and III subunit RPABC3 / RNA polymerase


Mass: 17162.273 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Sus scrofa (pig) / References: UniProt: A0A4X1ULF2
#63: Protein DNA-directed RNA polymerase II subunit RPB9 / Polymerase / RNA polymerase II subunit B9 / DNA-directed RNA polymerase II subunit I / RNA polymerase II 14.5 ...RNA polymerase II subunit B9 / DNA-directed RNA polymerase II subunit I / RNA polymerase II 14.5 kDa subunit / RPB14.5


Mass: 14541.221 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Sus scrofa (pig) / References: UniProt: P60899

+
Non-polymers , 3 types, 18 molecules

#71: Chemical
ChemComp-ZN / ZINC ION / Zinc


Mass: 65.409 Da / Num. of mol.: 16 / Source method: obtained synthetically / Formula: Zn
#72: Chemical ChemComp-SF4 / IRON/SULFUR CLUSTER / Iron–sulfur cluster


Mass: 351.640 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: Fe4S4
#73: Chemical ChemComp-MG / MAGNESIUM ION / Magnesium


Mass: 24.305 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: Mg

+
Details

Has ligand of interestN

-
Experimental details

-
Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

-
Sample preparation

ComponentName: TFIID-based PIC-Mediator holo-complex in fully-assembled state (hPIC-MED)
Type: COMPLEX / Entity ID: #1-#70 / Source: MULTIPLE SOURCES
Source (natural)Organism: Homo sapiens (human)
Source (recombinant)Organism: Homo sapiens (human)
Buffer solutionpH: 7.9
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
VitrificationCryogen name: ETHANE

-
Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELDBright-field microscopy
Image recordingElectron dose: 50 e/Å2 / Film or detector model: GATAN K2 SUMMIT (4k x 4k)

-
Processing

CTF correctionType: NONE
3D reconstructionResolution: 4.13 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 67732 / Symmetry type: POINT

+
About Yorodumi

-
News

-
Aug 12, 2020. Covid-19 info

Covid-19 info

URL: https://pdbj.org/emnavi/covid19.php

New page: Covid-19 featured information page in EM Navigator.

Related info.:Covid-19 info / Mar 5, 2020. Novel coronavirus structure data

-
Mar 5, 2020. Novel coronavirus structure data

Novel coronavirus structure data

Related info.:Yorodumi Speices / Aug 12, 2020. Covid-19 info

External links:COVID-19 featured content - PDBj / Molecule of the Month (242):Coronavirus Proteases

+
Jan 31, 2019. EMDB accession codes are about to change! (news from PDBe EMDB page)

EMDB accession codes are about to change! (news from PDBe EMDB page)

  • The allocation of 4 digits for EMDB accession codes will soon come to an end. Whilst these codes will remain in use, new EMDB accession codes will include an additional digit and will expand incrementally as the available range of codes is exhausted. The current 4-digit format prefixed with “EMD-” (i.e. EMD-XXXX) will advance to a 5-digit format (i.e. EMD-XXXXX), and so on. It is currently estimated that the 4-digit codes will be depleted around Spring 2019, at which point the 5-digit format will come into force.
  • The EM Navigator/Yorodumi systems omit the EMD- prefix.

Related info.:Q: What is EMD? / ID/Accession-code notation in Yorodumi/EM Navigator

External links:EMDB Accession Codes are Changing Soon! / Contact to PDBj

+
Jul 12, 2017. Major update of PDB

Major update of PDB

  • wwPDB released updated PDB data conforming to the new PDBx/mmCIF dictionary.
  • This is a major update changing the version number from 4 to 5, and with Remediation, in which all the entries are updated.
  • In this update, many items about electron microscopy experimental information are reorganized (e.g. em_software).
  • Now, EM Navigator and Yorodumi are based on the updated data.

External links:wwPDB Remediation / Enriched Model Files Conforming to OneDep Data Standards Now Available in the PDB FTP Archive

+
Jun 16, 2017. Omokage search with filter

Omokage search with filter

Result of Omokage search can be filtered by keywords and the database types

Related info.:Omokage search

-
Yorodumi

Thousand views of thousand structures

  • Yorodumi is a browser for structure data from EMDB, PDB, SASBDB, etc.
  • This page is also the successor to EM Navigator detail page, and also detail information page/front-end page for Omokage search.
  • The word "yorodu" (or yorozu) is an old Japanese word meaning "ten thousand". "mi" (miru) is to see.

Related info.:EMDB / PDB / SASBDB / Comparison of 3 databanks / Yorodumi Search / Aug 31, 2016. New EM Navigator & Yorodumi / Yorodumi Papers / Jmol/JSmol / Function and homology information / Changes in new EM Navigator and Yorodumi

Read more