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- EMDB-31191: Human Mediator (deletion of MED1-IDR) in a Tail-extended conformation -

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Open data


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Basic information

Entry
Database: EMDB / ID: EMD-31191
TitleHuman Mediator (deletion of MED1-IDR) in a Tail-extended conformation
Map data
SampleStructure of the human Mediator complex
  • (Mediator of RNA polymerase II transcription subunit ...) x 24
  • Unknown Chain (poly A)
  • (Isoform 2 of Mediator of RNA polymerase II transcription subunit ...) x 2
  • ligand
Function / homology
Function and homology information


positive regulation of mediator complex assembly / negative regulation of smooth muscle cell differentiation / core mediator complex / enucleate erythrocyte development / positive regulation of interferon-gamma-mediated signaling pathway / positive regulation of G0 to G1 transition / mammary gland branching involved in thelarche / retinal pigment epithelium development / regulation of RNA biosynthetic process / androgen biosynthetic process ...positive regulation of mediator complex assembly / negative regulation of smooth muscle cell differentiation / core mediator complex / enucleate erythrocyte development / positive regulation of interferon-gamma-mediated signaling pathway / positive regulation of G0 to G1 transition / mammary gland branching involved in thelarche / retinal pigment epithelium development / regulation of RNA biosynthetic process / androgen biosynthetic process / thyroid hormone mediated signaling pathway / embryonic heart tube development / regulation of vitamin D receptor signaling pathway / mRNA transcription by RNA polymerase II / thyroid hormone generation / retinoic acid receptor binding / limb development / ventricular trabecula myocardium morphogenesis / peroxisome proliferator activated receptor binding / embryonic hindlimb morphogenesis / positive regulation of keratinocyte differentiation / Generic Transcription Pathway / positive regulation of hepatocyte proliferation / mediator complex / embryonic placenta development / megakaryocyte development / thyroid hormone receptor binding / vitamin D receptor binding / cortical actin cytoskeleton / lens development in camera-type eye / cellular response to steroid hormone stimulus / cellular response to thyroid hormone stimulus / stem cell population maintenance / embryonic hemopoiesis / fat cell differentiation / positive regulation of intracellular estrogen receptor signaling pathway / erythrocyte development / positive regulation of transcription initiation from RNA polymerase II promoter / epithelial cell proliferation involved in mammary gland duct elongation / mammary gland branching involved in pregnancy / cellular response to hepatocyte growth factor stimulus / LBD domain binding / peroxisome proliferator activated receptor signaling pathway / positive regulation of chromatin binding / negative regulation of keratinocyte proliferation / termination of RNA polymerase II transcription / monocyte differentiation / negative regulation of neuron differentiation / ubiquitin ligase complex / negative regulation of fibroblast proliferation / regulation of transcription initiation from RNA polymerase II promoter / transcription coactivator binding / intracellular steroid hormone receptor signaling pathway / keratinocyte differentiation / retinoid X receptor binding / regulation of cell cycle => GO:0051726 / cellular response to epidermal growth factor stimulus / nuclear receptor binding / catalytic activity / RORA activates gene expression / Regulation of lipid metabolism by PPARalpha / lactation / nuclear receptor binding => GO:0016922 / promoter-specific chromatin binding / nuclear receptor coactivator activity / animal organ regeneration / BMAL1:CLOCK,NPAS2 activates circadian gene expression / Activation of gene expression by SREBF (SREBP) / cell morphogenesis / DNA-directed 5'-3' RNA polymerase activity / positive regulation of erythrocyte differentiation / protein-DNA complex / chromatin DNA binding / transcription coregulator activity / protein import into nucleus / transcription by RNA polymerase II / estrogen receptor binding / Transcriptional activation of mitochondrial biogenesis / ubiquitin protein ligase activity / transcription corepressor activity / liver development / Transcriptional regulation of white adipocyte differentiation / PPARA activates gene expression / regulation of lipid metabolic process / Nuclear Receptor transcription pathway / transcription regulator complex / brain development / Circadian Clock / actin binding / angiogenesis / transcription initiation from RNA polymerase II promoter / Estrogen-dependent gene expression / transcription coactivator activity / nuclear body / protein ubiquitination / transcription, DNA-templated / RNA polymerase II cis-regulatory region sequence-specific DNA binding / chromatin / transcription factor binding / chromatin binding
Similarity search - Function
Mediator complex subunit 26 C-terminal / Mediator complex subunit 26 middle domain / Mediator of RNA polymerase II transcription subunit 26 / Mediator complex subunit 26, middle domain / Mediator complex subunit 26, C-terminal domain / Mediator complex, subunit Med25, synapsin 1 / Mediator complex subunit 25 von Willebrand factor type A / Mediator complex, subunit Med25, von Willebrand factor type A / Mediator complex subunit 25 PTOV activation and synapsin 2 / Mediator complex subunit 25 synapsin 1 ...Mediator complex subunit 26 C-terminal / Mediator complex subunit 26 middle domain / Mediator of RNA polymerase II transcription subunit 26 / Mediator complex subunit 26, middle domain / Mediator complex subunit 26, C-terminal domain / Mediator complex, subunit Med25, synapsin 1 / Mediator complex subunit 25 von Willebrand factor type A / Mediator complex, subunit Med25, von Willebrand factor type A / Mediator complex subunit 25 PTOV activation and synapsin 2 / Mediator complex subunit 25 synapsin 1 / Mediator complex subunit 25 C-terminal NR box-containing / Mediator complex subunit 25, PTOV domain superfamily / Mediator complex, subunit Med25, NR box / Mediator complex, subunit Med25, PTOV domain / Mediator complex, subunit Med24, / Mediator complex subunit 29 / Mediator complex subunit 24 N-terminal / Mediator complex, subunit Med29, metazoa / Mediator complex, subunit Med23 / Mediator of RNA polymerase II transcription subunit 15 / ARC105 or Med15 subunit of Mediator complex non-fungal / Mediator complex subunit 23 / Mediator complex, subunit Med16 / Mediator complex subunit 16 / Mediator complex, subunit Med28 / Mediator of RNA polymerase II transcription subunit 9, metazoan / Mediator of RNA pol II transcription subunit 19 / Mediator complex, subunit Med6, metazoa/plant / Mediator complex, subunit Med30, metazoa / Mediator complex, subunit Med19, metazoa / Mediator complex subunit 30 / Mediator complex, subunit Med27 / Transcription factor subunit Med10 of Mediator complex / Mediator complex, subunit Med10 / Mediator complex subunit 27 / RNA polymerase II transcription mediator complex subunit 9 / Mediator of RNA polymerase II transcription subunit 9 / Mediator complex, subunit Med4 / Vitamin-D-receptor interacting Mediator subunit 4 / Mediator complex subunit MED14 / Mediator complex, subunit Med14 / Subunit 21 of Mediator complex / Mediator complex, subunit Med31 / SOH1 / Mediator complex, subunit Med21 / Mediator of RNA polymerase II, subunit Med31 superfamily / Mediator complex protein / Mediator complex, subunit Med11 / Mediator complex, subunit Med7 / MED7 protein / Mediator complex, subunit Med7/Med21-like / Mediator complex, subunit Med20 / TATA-binding related factor (TRF) of subunit 20 of Mediator complex / Subunit 17 of Mediator complex / MED6 mediator sub complex component / Mediator complex, subunit Med6 superfamily / Mediator complex, subunit Med17 / Mediator complex, subunit Med6 / Soluble quinoprotein glucose/sorbosone dehydrogenase / : / Surfeit locus protein 5 subunit 22 of Mediator complex / Mediator of RNA polymerase II transcription subunit 22 / Mediator of RNA polymerase II transcription complex subunit 8 / Mediator complex, subunit Med8, fungi/metazoa / Med18 protein / Mediator complex, subunit Med18 / Transcription elongation factor, TFIIS/CRSP70, N-terminal, sub-type / Domain in the N-terminus of transcription elongation factor S-II (and elsewhere) / TFIIS helical bundle-like domain / Transcription factor IIS, N-terminal / TFIIS N-terminal domain profile. / Mediator complex, subunit Med1 / Mediator of RNA polymerase II transcription subunit 1 / TFIIS/LEDGF domain superfamily / Coactivator CBP, KIX domain superfamily / von Willebrand factor A-like domain superfamily / Trp-Asp (WD) repeats profile. / Trp-Asp (WD) repeats circular profile. / WD40-repeat-containing domain / WD40 repeats / WD40 repeat / WD40-repeat-containing domain superfamily / WD40/YVTN repeat-like-containing domain superfamily
Similarity search - Domain/homology
Mediator of RNA polymerase II transcription subunit 4 / Mediator of RNA polymerase II transcription subunit 18 / Mediator of RNA polymerase II transcription subunit 28 / Mediator of RNA polymerase II transcription subunit 20 / Mediator of RNA polymerase II transcription subunit 29 / Mediator of RNA polymerase II transcription subunit 17 / Mediator of RNA polymerase II transcription subunit 9 / Mediator of RNA polymerase II transcription subunit 15 / Mediator of RNA polymerase II transcription subunit 11 / Mediator of RNA polymerase II transcription subunit 23 ...Mediator of RNA polymerase II transcription subunit 4 / Mediator of RNA polymerase II transcription subunit 18 / Mediator of RNA polymerase II transcription subunit 28 / Mediator of RNA polymerase II transcription subunit 20 / Mediator of RNA polymerase II transcription subunit 29 / Mediator of RNA polymerase II transcription subunit 17 / Mediator of RNA polymerase II transcription subunit 9 / Mediator of RNA polymerase II transcription subunit 15 / Mediator of RNA polymerase II transcription subunit 11 / Mediator of RNA polymerase II transcription subunit 23 / Mediator of RNA polymerase II transcription subunit 16 / Mediator of RNA polymerase II transcription subunit 10 / Mediator of RNA polymerase II transcription subunit 25 / Mediator of RNA polymerase II transcription subunit 30 / Mediator of RNA polymerase II transcription subunit 8 / Mediator of RNA polymerase II transcription subunit 27 / Mediator of RNA polymerase II transcription subunit 1 / Mediator of RNA polymerase II transcription subunit 22 / Mediator of RNA polymerase II transcription subunit 21 / Mediator of RNA polymerase II transcription subunit 26 / Mediator of RNA polymerase II transcription subunit 6 / Mediator of RNA polymerase II transcription subunit 24 / Mediator of RNA polymerase II transcription subunit 14 / Mediator of RNA polymerase II transcription subunit 7 / Mediator of RNA polymerase II transcription subunit 19 / Mediator of RNA polymerase II transcription subunit 31
Similarity search - Component
Biological speciesHomo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.5 Å
AuthorsYin X / Li J / Wu Z / Liu W / Xu Y
CitationJournal: Science / Year: 2021
Title: Structures of the human Mediator and Mediator-bound preinitiation complex.
Authors: Xizi Chen / Xiaotong Yin / Jiabei Li / Zihan Wu / Yilun Qi / Xinxin Wang / Weida Liu / Yanhui Xu /
Abstract: The 1.3-megadalton transcription factor IID (TFIID) is required for preinitiation complex (PIC) assembly and RNA polymerase II (Pol II)-mediated transcription initiation on almost all genes. The 26- ...The 1.3-megadalton transcription factor IID (TFIID) is required for preinitiation complex (PIC) assembly and RNA polymerase II (Pol II)-mediated transcription initiation on almost all genes. The 26-subunit Mediator stimulates transcription and cyclin-dependent kinase 7 (CDK7)-mediated phosphorylation of the Pol II C-terminal domain (CTD). We determined the structures of human Mediator in the Tail module-extended (at near-atomic resolution) and Tail-bent conformations and structures of TFIID-based PIC-Mediator (76 polypeptides, ~4.1 megadaltons) in four distinct conformations. PIC-Mediator assembly induces concerted reorganization (Head-tilting and Middle-down) of Mediator and creates a Head-Middle sandwich, which stabilizes two CTD segments and brings CTD to CDK7 for phosphorylation; this suggests a CTD-gating mechanism favorable for phosphorylation. The TFIID-based PIC architecture modulates Mediator organization and TFIIH stabilization, underscoring the importance of TFIID in orchestrating PIC-Mediator assembly.
History
DepositionApr 13, 2021-
Header (metadata) releaseMay 5, 2021-
Map releaseMay 5, 2021-
UpdateMay 19, 2021-
Current statusMay 19, 2021Processing site: PDBj / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.01
  • Imaged by UCSF Chimera
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  • Surface view colored by radius
  • Surface level: 0.01
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-7emf
  • Surface level: 0.01
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

Downloads & links

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Map

FileDownload / File: emd_31191.map.gz / Format: CCP4 / Size: 512 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.05 Å/pix.
x 512 pix.
= 539.648 Å
1.05 Å/pix.
x 512 pix.
= 539.648 Å
1.05 Å/pix.
x 512 pix.
= 539.648 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.054 Å
Density
Contour LevelBy AUTHOR: 0.01 / Movie #1: 0.01
Minimum - Maximum-0.0638854 - 0.114047185
Average (Standard dev.)9.998839e-05 (±0.0015914931)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions512512512
Spacing512512512
CellA=B=C: 539.648 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.0541.0541.054
M x/y/z512512512
origin x/y/z0.0000.0000.000
length x/y/z539.648539.648539.648
α/β/γ90.00090.00090.000
start NX/NY/NZ000
NX/NY/NZ512512512
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS512512512
D min/max/mean-0.0640.1140.000

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Supplemental data

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Sample components

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Entire Structure of the human Mediator complex

EntireName: Structure of the human Mediator complex / Number of components: 29

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Component #1: protein, Structure of the human Mediator complex

ProteinName: Structure of the human Mediator complex / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (engineered)Expression System: Homo sapiens (human) / Cell of expression system: Expi293

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Component #2: protein, Mediator of RNA polymerase II transcription subunit 1

ProteinName: Mediator of RNA polymerase II transcription subunit 1 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 168.697328 kDa
SourceSpecies: Homo sapiens (human)
Source (engineered)Expression System: Homo sapiens (human)

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Component #3: protein, Unknown Chain (poly A)

ProteinName: Unknown Chain (poly A) / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 1.439571 kDa
SourceSpecies: Homo sapiens (human)
Source (engineered)Expression System: Homo sapiens (human)

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Component #4: protein, Mediator of RNA polymerase II transcription subunit 4

ProteinName: Mediator of RNA polymerase II transcription subunit 4 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 29.781424 kDa
SourceSpecies: Homo sapiens (human)
Source (engineered)Expression System: Homo sapiens (human)

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Component #5: protein, Mediator of RNA polymerase II transcription subunit 6

ProteinName: Mediator of RNA polymerase II transcription subunit 6 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 28.464627 kDa
SourceSpecies: Homo sapiens (human)
Source (engineered)Expression System: Homo sapiens (human)

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Component #6: protein, Mediator of RNA polymerase II transcription subunit 7

ProteinName: Mediator of RNA polymerase II transcription subunit 7 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 27.285281 kDa
SourceSpecies: Homo sapiens (human)
Source (engineered)Expression System: Homo sapiens (human)

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Component #7: protein, Isoform 2 of Mediator of RNA polymerase II transcription...

ProteinName: Isoform 2 of Mediator of RNA polymerase II transcription subunit 8
Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 29.112906 kDa
SourceSpecies: Homo sapiens (human)
Source (engineered)Expression System: Homo sapiens (human)

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Component #8: protein, Mediator of RNA polymerase II transcription subunit 9

ProteinName: Mediator of RNA polymerase II transcription subunit 9 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 16.42275 kDa
SourceSpecies: Homo sapiens (human)
Source (engineered)Expression System: Homo sapiens (human)

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Component #9: protein, Mediator of RNA polymerase II transcription subunit 10

ProteinName: Mediator of RNA polymerase II transcription subunit 10
Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 15.710969 kDa
SourceSpecies: Homo sapiens (human)
Source (engineered)Expression System: Homo sapiens (human)

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Component #10: protein, Mediator of RNA polymerase II transcription subunit 11

ProteinName: Mediator of RNA polymerase II transcription subunit 11
Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 13.145829 kDa
SourceSpecies: Homo sapiens (human)
Source (engineered)Expression System: Homo sapiens (human)

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Component #11: protein, Mediator of RNA polymerase II transcription subunit 14

ProteinName: Mediator of RNA polymerase II transcription subunit 14
Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 160.787594 kDa
SourceSpecies: Homo sapiens (human)
Source (engineered)Expression System: Homo sapiens (human)

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Component #12: protein, Mediator of RNA polymerase II transcription subunit 15

ProteinName: Mediator of RNA polymerase II transcription subunit 15
Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 86.830102 kDa
SourceSpecies: Homo sapiens (human)
Source (engineered)Expression System: Homo sapiens (human)

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Component #13: protein, Isoform 2 of Mediator of RNA polymerase II transcription...

ProteinName: Isoform 2 of Mediator of RNA polymerase II transcription subunit 16
Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 93.136039 kDa
SourceSpecies: Homo sapiens (human)
Source (engineered)Expression System: Homo sapiens (human)

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Component #14: protein, Mediator of RNA polymerase II transcription subunit 17

ProteinName: Mediator of RNA polymerase II transcription subunit 17
Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 72.984047 kDa
SourceSpecies: Homo sapiens (human)
Source (engineered)Expression System: Homo sapiens (human)

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Component #15: protein, Mediator of RNA polymerase II transcription subunit 18

ProteinName: Mediator of RNA polymerase II transcription subunit 18
Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 23.69373 kDa
SourceSpecies: Homo sapiens (human)
Source (engineered)Expression System: Homo sapiens (human)

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Component #16: protein, Mediator of RNA polymerase II transcription subunit 19

ProteinName: Mediator of RNA polymerase II transcription subunit 19
Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 26.321146 kDa
SourceSpecies: Homo sapiens (human)
Source (engineered)Expression System: Homo sapiens (human)

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Component #17: protein, Mediator of RNA polymerase II transcription subunit 20

ProteinName: Mediator of RNA polymerase II transcription subunit 20
Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 23.243762 kDa
SourceSpecies: Homo sapiens (human)
Source (engineered)Expression System: Homo sapiens (human)

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Component #18: protein, Mediator of RNA polymerase II transcription subunit 21

ProteinName: Mediator of RNA polymerase II transcription subunit 21
Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 15.575234 kDa
SourceSpecies: Homo sapiens (human)
Source (engineered)Expression System: Homo sapiens (human)

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Component #19: protein, Mediator of RNA polymerase II transcription subunit 22

ProteinName: Mediator of RNA polymerase II transcription subunit 22
Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 22.243713 kDa
SourceSpecies: Homo sapiens (human)
Source (engineered)Expression System: Homo sapiens (human)

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Component #20: protein, Mediator of RNA polymerase II transcription subunit 23

ProteinName: Mediator of RNA polymerase II transcription subunit 23
Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 156.652156 kDa
SourceSpecies: Homo sapiens (human)
Source (engineered)Expression System: Homo sapiens (human)

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Component #21: protein, Mediator of RNA polymerase II transcription subunit 24

ProteinName: Mediator of RNA polymerase II transcription subunit 24
Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 110.430164 kDa
SourceSpecies: Homo sapiens (human)
Source (engineered)Expression System: Homo sapiens (human)

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Component #22: protein, Mediator of RNA polymerase II transcription subunit 25

ProteinName: Mediator of RNA polymerase II transcription subunit 25
Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 78.230344 kDa
SourceSpecies: Homo sapiens (human)
Source (engineered)Expression System: Homo sapiens (human)

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Component #23: protein, Mediator of RNA polymerase II transcription subunit 26

ProteinName: Mediator of RNA polymerase II transcription subunit 26
Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 65.544438 kDa
SourceSpecies: Homo sapiens (human)
Source (engineered)Expression System: Homo sapiens (human)

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Component #24: protein, Mediator of RNA polymerase II transcription subunit 27

ProteinName: Mediator of RNA polymerase II transcription subunit 27
Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 35.478742 kDa
SourceSpecies: Homo sapiens (human)
Source (engineered)Expression System: Homo sapiens (human)

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Component #25: protein, Mediator of RNA polymerase II transcription subunit 28

ProteinName: Mediator of RNA polymerase II transcription subunit 28
Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 19.540107 kDa
SourceSpecies: Homo sapiens (human)
Source (engineered)Expression System: Homo sapiens (human)

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Component #26: protein, Mediator of RNA polymerase II transcription subunit 29

ProteinName: Mediator of RNA polymerase II transcription subunit 29
Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 21.089801 kDa
SourceSpecies: Homo sapiens (human)
Source (engineered)Expression System: Homo sapiens (human)

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Component #27: protein, Mediator of RNA polymerase II transcription subunit 30

ProteinName: Mediator of RNA polymerase II transcription subunit 30
Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 20.301229 kDa
SourceSpecies: Homo sapiens (human)
Source (engineered)Expression System: Homo sapiens (human)

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Component #28: protein, Mediator of RNA polymerase II transcription subunit 31

ProteinName: Mediator of RNA polymerase II transcription subunit 31
Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 15.826116 kDa
SourceSpecies: Homo sapiens (human)
Source (engineered)Expression System: Homo sapiens (human)

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Component #29: ligand, ZINC ION

LigandName: ZINC ION / Number of Copies: 2 / Recombinant expression: No
MassTheoretical: 6.540905 MDa

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Experimental details

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Sample preparation

SpecimenSpecimen state: Particle / Method: cryo EM
Sample solutionpH: 8
VitrificationInstrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Humidity: 100 %

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
ImagingMicroscope: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Electron dose: 50 e/Å2 / Illumination mode: FLOOD BEAM
LensCs: 2.7 mm / Imaging mode: BRIGHT FIELD
Specimen HolderModel: FEI TITAN KRIOS AUTOGRID HOLDER
CameraDetector: GATAN K2 SUMMIT (4k x 4k)

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Image processing

ProcessingMethod: single particle reconstruction / Number of projections: 95433
3D reconstructionResolution: 3.5 Å / Resolution method: FSC 0.143 CUT-OFF

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Atomic model buiding

Modeling #1Refinement space: REAL
Output model

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