+
Open data
-
Basic information
Entry | Database: EMDB / ID: EMD-6827 | |||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Title | Cryo-EM Structure of the Exocyst Complex | |||||||||||||||
![]() | Cryo-EM map of the yeast Exocyst complex overall structure at 5.5A resolution | |||||||||||||||
![]() |
| |||||||||||||||
![]() | exocyst / coiled-coil / EXOCYTOSIS | |||||||||||||||
Function / homology | ![]() vesicle tethering involved in exocytosis / exocyst assembly / exocyst localization / negative regulation of SNARE complex assembly / endoplasmic reticulum inheritance / exocyst / prospore membrane / Golgi inheritance / incipient cellular bud site / cellular bud tip ...vesicle tethering involved in exocytosis / exocyst assembly / exocyst localization / negative regulation of SNARE complex assembly / endoplasmic reticulum inheritance / exocyst / prospore membrane / Golgi inheritance / incipient cellular bud site / cellular bud tip / Golgi to plasma membrane transport / cellular bud neck / mating projection tip / vesicle docking involved in exocytosis / exocytosis / spliceosomal complex assembly / Rho protein signal transduction / transport vesicle / phosphatidylinositol-4,5-bisphosphate binding / SNARE binding / cell periphery / intracellular protein transport / protein localization / small GTPase binding / protein transport / plasma membrane / cytoplasm Similarity search - Function | |||||||||||||||
Biological species | ![]() ![]() ![]() ![]() | |||||||||||||||
Method | single particle reconstruction / cryo EM / Resolution: 4.4 Å | |||||||||||||||
![]() | Mei K / Li Y | |||||||||||||||
Funding support | ![]() ![]()
| |||||||||||||||
![]() | ![]() Title: Cryo-EM structure of the exocyst complex. Authors: Kunrong Mei / Yan Li / Shaoxiao Wang / Guangcan Shao / Jia Wang / Yuehe Ding / Guangzuo Luo / Peng Yue / Jun-Jie Liu / Xinquan Wang / Meng-Qiu Dong / Hong-Wei Wang / Wei Guo / ![]() ![]() Abstract: The exocyst is an evolutionarily conserved octameric protein complex that mediates the tethering of post-Golgi secretory vesicles to the plasma membrane during exocytosis and is implicated in many ...The exocyst is an evolutionarily conserved octameric protein complex that mediates the tethering of post-Golgi secretory vesicles to the plasma membrane during exocytosis and is implicated in many cellular processes such as cell polarization, cytokinesis, ciliogenesis and tumor invasion. Using cryo-EM and chemical cross-linking MS (CXMS), we solved the structure of the Saccharomyces cerevisiae exocyst complex at an average resolution of 4.4 Å. Our model revealed the architecture of the exocyst and led to the identification of the helical bundles that mediate the assembly of the complex at its core. Sequence analysis suggests that these regions are evolutionarily conserved across eukaryotic systems. Additional cell biological data suggest a mechanism for exocyst assembly that leads to vesicle tethering at the plasma membrane. | |||||||||||||||
History |
|
-
Structure visualization
Movie |
![]() |
---|---|
Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
-
Downloads & links
-EMDB archive
Map data | ![]() | 12.6 MB | ![]() | |
---|---|---|---|---|
Header (meta data) | ![]() ![]() | 57.7 KB 57.7 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() ![]() ![]() ![]() ![]() ![]() | 12.7 KB 12.7 KB 12.8 KB 12.8 KB 12.7 KB 12.7 KB | Display Display Display Display Display Display | ![]() |
Images | ![]() ![]() ![]() ![]() ![]() ![]() | 41.6 KB 41.6 KB 32.4 KB 27.3 KB 30.4 KB 47 KB | ||
Masks | ![]() ![]() ![]() ![]() ![]() | 178 MB 178 MB 178 MB 178 MB 178 MB | ![]() | |
Filedesc metadata | ![]() | 12.8 KB | ||
Others | ![]() ![]() ![]() ![]() | 9.8 MB 9.7 MB 14.4 MB 13.7 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 471.5 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 471 KB | Display | |
Data in XML | ![]() | 13.1 KB | Display | |
Data in CIF | ![]() | 17.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 5yfpMC M: atomic model generated by this map C: citing same article ( |
---|---|
Similar structure data | |
EM raw data | ![]() Data size: 10.0 TB Data #1: Unaligned multi-frame micrographs of Yeast Exocyst complex [micrographs - multiframe]) |
-
Links
EMDB pages | ![]() ![]() |
---|---|
Related items in Molecule of the Month |
-
Map
File | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Annotation | Cryo-EM map of the yeast Exocyst complex overall structure at 5.5A resolution | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.30654 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
|
-Supplemental data
-Mask #1
File | ![]() | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Projections & Slices |
| ||||||||||||
Density Histograms |
-Mask #2
File | ![]() | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Projections & Slices |
| ||||||||||||
Density Histograms |
-Mask #3
File | ![]() | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Projections & Slices |
| ||||||||||||
Density Histograms |
-Mask #4
File | ![]() | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Projections & Slices |
| ||||||||||||
Density Histograms |
-Mask #5
File | ![]() | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Projections & Slices |
| ||||||||||||
Density Histograms |
-Additional map: Cryo-EM map of the yeast Exocyst complex head-part...
File | emd_6827_additional_1.map | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Annotation | Cryo-EM map of the yeast Exocyst complex head-part structure at 6.7A resolution | ||||||||||||
Projections & Slices |
| ||||||||||||
Density Histograms |
-Additional map: Cryo-EM map of the yeast Exocyst complex top...
File | emd_6827_additional_2.map | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Annotation | Cryo-EM map of the yeast Exocyst complex top head-part structure at 6.2A resolution | ||||||||||||
Projections & Slices |
| ||||||||||||
Density Histograms |
-Additional map: Cryo-EM map of the yeast Exocyst complex bottom...
File | emd_6827_additional_3.map | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Annotation | Cryo-EM map of the yeast Exocyst complex bottom leg-part structure at 4.6A resolution | ||||||||||||
Projections & Slices |
| ||||||||||||
Density Histograms |
-Additional map: Cryo-EM map of the yeast Exocyst complex body-part...
File | emd_6827_additional_4.map | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Annotation | Cryo-EM map of the yeast Exocyst complex body-part structure at 4.4A resolution | ||||||||||||
Projections & Slices |
| ||||||||||||
Density Histograms |