Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Structures of the human Mediator and Mediator-bound preinitiation complex.
Journal, issue, pages	Science, Vol. 372, Issue 6546, Year 2021
Publish date	Jun 4, 2021
Authors	Xizi Chen / Xiaotong Yin / Jiabei Li / Zihan Wu / Yilun Qi / Xinxin Wang / Weida Liu / Yanhui Xu /
PubMed Abstract	The 1.3-megadalton transcription factor IID (TFIID) is required for preinitiation complex (PIC) assembly and RNA polymerase II (Pol II)-mediated transcription initiation on almost all genes. The 26- ...The 1.3-megadalton transcription factor IID (TFIID) is required for preinitiation complex (PIC) assembly and RNA polymerase II (Pol II)-mediated transcription initiation on almost all genes. The 26-subunit Mediator stimulates transcription and cyclin-dependent kinase 7 (CDK7)-mediated phosphorylation of the Pol II C-terminal domain (CTD). We determined the structures of human Mediator in the Tail module-extended (at near-atomic resolution) and Tail-bent conformations and structures of TFIID-based PIC-Mediator (76 polypeptides, ~4.1 megadaltons) in four distinct conformations. PIC-Mediator assembly induces concerted reorganization (Head-tilting and Middle-down) of Mediator and creates a Head-Middle sandwich, which stabilizes two CTD segments and brings CTD to CDK7 for phosphorylation; this suggests a CTD-gating mechanism favorable for phosphorylation. The TFIID-based PIC architecture modulates Mediator organization and TFIIH stabilization, underscoring the importance of TFIID in orchestrating PIC-Mediator assembly.
External links	Science / PubMed:33958484
Methods	EM (single particle)
Resolution	2.8 - 7.1 Å
Structure data	31191 7emf EMDB-31191, PDB-7emf: Human Mediator (deletion of MED1-IDR) in a Tail-extended conformation Method: EM (single particle) / Resolution: 3.5 Å EMDB-31192: Human Mediator (deletion of MED1-IDR) Head module Method: EM (single particle) / Resolution: 2.8 Å EMDB-31193: Human Mediator (deletion of MED1-IDR) Middle module Method: EM (single particle) / Resolution: 3.7 Å EMDB-31194: Human Mediator (deletion of MED1-IDR) Tail module Method: EM (single particle) / Resolution: 3.2 Å EMDB-31195: Structure of the human Mediator MED1 module Method: EM (single particle) / Resolution: 3.6 Å EMDB-31196: Human Mediator (deletion of MED1-IDR) Head module near MED6 Method: EM (single particle) / Resolution: 3.1 Å 31204 7ena EMDB-31204, PDB-7ena: TFIID-based PIC-Mediator holo-complex in pre-assembled state (pre-hPIC-MED) Method: EM (single particle) / Resolution: 4.07 Å EMDB-31205: TFIID-based PIC-MED holo-complex (deletion of MED1-IDR) in an intermediate state (state2) Method: EM (single particle) / Resolution: 4.0 Å EMDB-31206: TFIID-based PIC-MED holo-complex (deletion of MED1-IDR) in an intermediate state (state3) Method: EM (single particle) / Resolution: 5.22 Å 31207 7enc EMDB-31207, PDB-7enc: TFIID-based PIC-Mediator holo-complex in fully-assembled state (hPIC-MED) Method: EM (single particle) / Resolution: 4.13 Å EMDB-31208: Local maps of TFIID-based PIC-Mediator complex (deletion of MED1-IDR) Method: EM (single particle) / Resolution: 4.54 Å 31211 7enj EMDB-31211, PDB-7enj: Human Mediator (deletion of MED1-IDR) in a Tail-bent conformation (MED-B) Method: EM (single particle) / Resolution: 4.4 Å EMDB-31212: Human Mediator (deletion of MED1-IDR) Tail module in a bent conformation Method: EM (single particle) / Resolution: 4.5 Å EMDB-31213: Human Mediator in a Tail-bent conformation (MED-B) Method: EM (single particle) / Resolution: 7.1 Å EMDB-31214: Human Mediator in a Tail-extended conformation (MED-E) Method: EM (single particle) / Resolution: 4.0 Å EMDB-31215: TFIID-based PIC-MED holo-complex Method: EM (single particle) / Resolution: 6.5 Å
Chemicals	ChemComp-ZN: Unknown entry ChemComp-MG: Unknown entry ChemComp-SF4: IRON/SULFUR CLUSTER
Source	homo sapiens (human) sus scrofa (pig)
Keywords	TRANSCRIPTION / Mediator complex / mediator / preinitiation complex / transcription initiation