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- EMDB-21514: map of the mammalian Mediator complex -

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Open data


ID or keywords:

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Basic information

Entry
Database: EMDB / ID: EMD-21514
Titlemap of the mammalian Mediator complex
Map dataMammalian Mediator complex
Sample
  • Complex: Mouse mediator complexMediator (coactivator)
    • Protein or peptide: x 25 types
Function / homology
Function and homology information


: / Generic Transcription Pathway / regulation of cell cycle => GO:0051726 / positive regulation of mediator complex assembly / positive regulation of T cell extravasation / negative regulation of smooth muscle cell differentiation / core mediator complex / mediator complex / nuclear retinoic acid receptor binding / positive regulation of chromatin binding ...: / Generic Transcription Pathway / regulation of cell cycle => GO:0051726 / positive regulation of mediator complex assembly / positive regulation of T cell extravasation / negative regulation of smooth muscle cell differentiation / core mediator complex / mediator complex / nuclear retinoic acid receptor binding / positive regulation of chromatin binding / limb development / nuclear thyroid hormone receptor binding / termination of RNA polymerase II transcription / cortical actin cytoskeleton / stem cell population maintenance / skeletal muscle cell differentiation / blastocyst development / transcription factor binding / positive regulation of transcription initiation by RNA polymerase II / ubiquitin ligase complex / nuclear retinoid X receptor binding / catalytic activity / negative regulation of fibroblast proliferation / histone acetyltransferase activity / nuclear receptor coactivator activity / transcription coregulator activity / transcription coactivator binding / ubiquitin protein ligase activity / actin binding / transcription regulator complex / transcription by RNA polymerase II / transcription coactivator activity / protein ubiquitination / nuclear body / cytoskeleton / RNA polymerase II cis-regulatory region sequence-specific DNA binding / protein-containing complex binding / positive regulation of gene expression / nucleolus / regulation of DNA-templated transcription / regulation of transcription by RNA polymerase II / positive regulation of DNA-templated transcription / negative regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / DNA binding / nucleoplasm / membrane / nucleus / cytosol / cytoplasm
Similarity search - Function
Mediator complex, subunit Med25, NR box / : / : / Mediator complex, subunit Med25, PTOV domain / Mediator complex, subunit Med25, synapsin 1 / Mediator of RNA polymerase II transcription subunit 25, von Willebrand factor type A domain / Mediator complex subunit 25, PTOV domain superfamily / Mediator complex subunit 25 PTOV activation and synapsin 2 / Mediator complex subunit 25 synapsin 1 / Mediator complex subunit 25 von Willebrand factor type A ...Mediator complex, subunit Med25, NR box / : / : / Mediator complex, subunit Med25, PTOV domain / Mediator complex, subunit Med25, synapsin 1 / Mediator of RNA polymerase II transcription subunit 25, von Willebrand factor type A domain / Mediator complex subunit 25, PTOV domain superfamily / Mediator complex subunit 25 PTOV activation and synapsin 2 / Mediator complex subunit 25 synapsin 1 / Mediator complex subunit 25 von Willebrand factor type A / Mediator complex, subunit Med29, metazoa / Mediator complex, subunit Med24, / Mediator complex subunit 24 N-terminal / Mediator complex subunit 29 / Mediator of RNA polymerase II transcription subunit 15 / Mediator complex, subunit Med23 / ARC105/Med15 mediator subunit N-terminal KIX domain / Mediator complex subunit 23 / Mediator complex, subunit Med6, metazoa/plant / Mediator complex, subunit Med19, metazoa / Mediator complex, subunit Med30, metazoa / Mediator complex, subunit Med28 / Mediator of RNA polymerase II transcription subunit 9, metazoan / Mediator of RNA pol II transcription subunit 19 / Mediator complex subunit 30 / Mediator complex, subunit Med27 / Mediator complex subunit 27 / Mediator complex, subunit Med16 / Mediator complex subunit 16, N-terminal / Mediator complex, subunit Med10 / Transcription factor subunit Med10 of Mediator complex / Mediator of RNA polymerase II transcription subunit 9 / RNA polymerase II transcription mediator complex subunit 9 / Mediator complex, subunit Med4 / Vitamin-D-receptor interacting Mediator subunit 4 / Mediator complex, subunit Med31 / Mediator complex, subunit Med21 / Mediator of RNA polymerase II, subunit Med31 superfamily / SOH1 / Subunit 21 of Mediator complex / Mediator complex, subunit Med7 / Soluble quinoprotein glucose/sorbosone dehydrogenase / Mediator complex, subunit Med7/Med21-like / MED7 protein / Mediator complex, subunit Med20 / Mediator complex, subunit Med14 / TATA-binding related factor (TRF) of subunit 20 of Mediator complex / Mediator complex subunit MED14 / Mediator complex, subunit Med6 / Mediator complex, subunit Med17 / Mediator complex, subunit Med6 superfamily / MED6 mediator sub complex component / Subunit 17 of Mediator complex / Mediator of RNA polymerase II transcription subunit 22 / Mediator complex, subunit Med8, fungi/metazoa / Mediator complex, subunit Med11 / Surfeit locus protein 5 subunit 22 of Mediator complex / Mediator of RNA polymerase II transcription complex subunit 8 / Mediator complex protein / Mediator complex, subunit Med18 / Med18 protein / : / Coactivator CBP, KIX domain superfamily / von Willebrand factor A-like domain superfamily / WD40 repeats / WD40 repeat / Trp-Asp (WD) repeats profile. / Trp-Asp (WD) repeats circular profile. / WD40-repeat-containing domain superfamily / WD40/YVTN repeat-like-containing domain superfamily
Similarity search - Domain/homology
Mediator of RNA polymerase II transcription subunit 14 / Mediator of RNA polymerase II transcription subunit 22 / Mediator of RNA polymerase II transcription subunit 16 / Mediator of RNA polymerase II transcription subunit 23 / Mediator of RNA polymerase II transcription subunit 19 / Mediator of RNA polymerase II transcription subunit 25 / Mediator of RNA polymerase II transcription subunit 17 / Mediator of RNA polymerase II transcription subunit 9 / Mediator of RNA polymerase II transcription subunit 28 / Mediator of RNA polymerase II transcription subunit 6 ...Mediator of RNA polymerase II transcription subunit 14 / Mediator of RNA polymerase II transcription subunit 22 / Mediator of RNA polymerase II transcription subunit 16 / Mediator of RNA polymerase II transcription subunit 23 / Mediator of RNA polymerase II transcription subunit 19 / Mediator of RNA polymerase II transcription subunit 25 / Mediator of RNA polymerase II transcription subunit 17 / Mediator of RNA polymerase II transcription subunit 9 / Mediator of RNA polymerase II transcription subunit 28 / Mediator of RNA polymerase II transcription subunit 6 / Mediator of RNA polymerase II transcription subunit 15 / Mediator of RNA polymerase II transcription subunit 24 / Mediator of RNA polymerase II transcription subunit 21 / Mediator of RNA polymerase II transcription subunit 4 / Mediator of RNA polymerase II transcription subunit 30 / Mediator of RNA polymerase II transcription subunit 10 / Mediator of RNA polymerase II transcription subunit 31 / Mediator of RNA polymerase II transcription subunit 18 / Mediator of RNA polymerase II transcription subunit 7 / Mediator of RNA polymerase II transcription subunit 8 / Mediator of RNA polymerase II transcription subunit 11 / Mediator of RNA polymerase II transcription subunit 27 / Mediator of RNA polymerase II transcription subunit 29 / Mediator of RNA polymerase II transcription subunit 20
Similarity search - Component
Biological speciesMus musculus (house mouse) / Mouse (mice)
Methodsingle particle reconstruction / cryo EM / Resolution: 4.02 Å
AuthorsZhao H / Young N / Asturias F
Funding support United States, 2 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R01-67167 United States
National Institutes of Health/National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIH/NIAMS) United States
CitationJournal: Cell / Year: 2019
Title: A Pliable Mediator Acts as a Functional Rather Than an Architectural Bridge between Promoters and Enhancers.
Authors: Laila El Khattabi / Haiyan Zhao / Jens Kalchschmidt / Natalie Young / Seolkyoung Jung / Peter Van Blerkom / Philippe Kieffer-Kwon / Kyong-Rim Kieffer-Kwon / Solji Park / Xiang Wang / Jordan ...Authors: Laila El Khattabi / Haiyan Zhao / Jens Kalchschmidt / Natalie Young / Seolkyoung Jung / Peter Van Blerkom / Philippe Kieffer-Kwon / Kyong-Rim Kieffer-Kwon / Solji Park / Xiang Wang / Jordan Krebs / Subhash Tripathi / Noboru Sakabe / Débora R Sobreira / Su-Chen Huang / Suhas S P Rao / Nathanael Pruett / Daniel Chauss / Erica Sadler / Andrea Lopez / Marcelo A Nóbrega / Erez Lieberman Aiden / Francisco J Asturias / Rafael Casellas /
Abstract: While Mediator plays a key role in eukaryotic transcription, little is known about its mechanism of action. This study combines CRISPR-Cas9 genetic screens, degron assays, Hi-C, and cryoelectron ...While Mediator plays a key role in eukaryotic transcription, little is known about its mechanism of action. This study combines CRISPR-Cas9 genetic screens, degron assays, Hi-C, and cryoelectron microscopy (cryo-EM) to dissect the function and structure of mammalian Mediator (mMED). Deletion analyses in B, T, and embryonic stem cells (ESC) identified a core of essential subunits required for Pol II recruitment genome-wide. Conversely, loss of non-essential subunits mostly affects promoters linked to multiple enhancers. Contrary to current models, however, mMED and Pol II are dispensable to physically tether regulatory DNA, a topological activity requiring architectural proteins. Cryo-EM analysis revealed a conserved core, with non-essential subunits increasing structural complexity of the tail module, a primary transcription factor target. Changes in tail structure markedly increase Pol II and kinase module interactions. We propose that Mediator's structural pliability enables it to integrate and transmit regulatory signals and act as a functional, rather than an architectural bridge, between promoters and enhancers.
History
DepositionMar 4, 2020-
Header (metadata) releaseMar 10, 2021-
Map releaseMar 10, 2021-
UpdateMar 10, 2021-
Current statusMar 10, 2021Processing site: RCSB / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.1
  • Imaged by UCSF Chimera
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  • Surface view colored by radius
  • Surface level: 0.1
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-6w1s
  • Surface level: 0.1
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_21514.png

Downloads & links

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Map

FileDownload / File: emd_21514.map.gz / Format: CCP4 / Size: 669.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationMammalian Mediator complex
Voxel sizeX=Y=Z: 1.11 Å
Density
Contour LevelBy AUTHOR: 0.1 / Movie #1: 0.1
Minimum - Maximum-0.39771584 - 0.66880524
Average (Standard dev.)0.00015942252 (±0.021543505)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions560560560
Spacing560560560
CellA=B=C: 621.60004 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.111.111.11
M x/y/z560560560
origin x/y/z0.0000.0000.000
length x/y/z621.600621.600621.600
α/β/γ90.00090.00090.000
start NX/NY/NZ-170-170-170
NX/NY/NZ340340340
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS560560560
D min/max/mean-0.3980.6690.000

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Supplemental data

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Additional map: Mammalian Mediator complex

Fileemd_21514_additional_1.map
AnnotationMammalian Mediator complex
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Additional map: Mammalian Mediator complex

Fileemd_21514_additional_2.map
AnnotationMammalian Mediator complex
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Mouse mediator complex

EntireName: Mouse mediator complexMediator (coactivator)
Components
  • Complex: Mouse mediator complexMediator (coactivator)
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 1
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 4
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 6
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 7
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 8
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 9
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 10
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 11
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 14
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 15
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 16
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 17
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 18
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 19
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 20
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 21
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 22
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 23
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 24
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 25
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 27
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 28
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 29
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 30
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 31

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Supramolecule #1: Mouse mediator complex

SupramoleculeName: Mouse mediator complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 1.0 MDa

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Macromolecule #1: Mediator of RNA polymerase II transcription subunit 1

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 1 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mouse (mice)
Molecular weightTheoretical: 32.527998 KDa
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)

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Macromolecule #2: Mediator of RNA polymerase II transcription subunit 4

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 4 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mouse (mice)
Molecular weightTheoretical: 11.647339 KDa
SequenceString:
LLSALEDLEV LSRELIEMLA ISRNQKLLQL EEENQVLELL IHRDGDFQEL MKLALNQGKV HHEMQALEKE VEKRDSDIQQ LQKQLKEAE QILATAVYQA K

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Macromolecule #3: Mediator of RNA polymerase II transcription subunit 6

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 6 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mouse (mice)
Molecular weightTheoretical: 28.473553 KDa
SequenceString: MAAVDIRDNL LGISWVDSSW IPILNSGSVL DYFSERSNPF YDRTCNNEVV KMQRLTLEHL NQMVGIEYIL LHAQEPILFI IRKQQRQSP AQVIPLADYY IIAGVIYQAP DLGSVINSRV LTAVHGIQSA FDEAMSYCRY HPSKGYWWHF KDHEEQEKVK P KAKRKEEP ...String:
MAAVDIRDNL LGISWVDSSW IPILNSGSVL DYFSERSNPF YDRTCNNEVV KMQRLTLEHL NQMVGIEYIL LHAQEPILFI IRKQQRQSP AQVIPLADYY IIAGVIYQAP DLGSVINSRV LTAVHGIQSA FDEAMSYCRY HPSKGYWWHF KDHEEQEKVK P KAKRKEEP SSIFQRQRVD ALLIDLRQKF PPRFVQQKSG EKPVPVDQAK KEAEPLPETV KSEEKESTKN IQQTVSTKGP PE KRMRLQ

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Macromolecule #4: Mediator of RNA polymerase II transcription subunit 7

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 7 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mouse (mice)
Molecular weightTheoretical: 18.836996 KDa
SequenceString:
PPPPMQYIKE YTDENIQEGL APKPPPPIKD SYMMFGNQFQ CDDLIIRPLE SQGIERLHPM QFDHKKELRK LNMSILINFL DLLDILIRS PGSIKREEKL EDLKLLFVHV HHLINEYRPH QARETLRVMM EVQKRQRLET AERFQKHLER VIEMIQNC

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Macromolecule #5: Mediator of RNA polymerase II transcription subunit 8

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 8 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mouse (mice)
Molecular weightTheoretical: 20.156684 KDa
SequenceString:
QLEASLDALL NQVADLKNSL GSFIYKLENE YDRLTWPSVL DSFALLSGQL NTLNKVLKHE KTPLFRNQVI IPLVLSPDRD EDLMRQTEG RVPVFSHEVV PDHLRTKPDP EVEEQEKQLT TDAARIGADA AQKQIQSLNK MCSNLLEKIS KEERESESGG L RPNKQTFN PGDTNALVAA VA

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Macromolecule #6: Mediator of RNA polymerase II transcription subunit 9

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 9 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mouse (mice)
Molecular weightTheoretical: 8.924443 KDa
SequenceString:
FLPLVHNVIK CMDKDSPDLH QDLNALKTKF QELRKLIGTM PGIHVSPEQQ QQQLHSLREQ VRTKNELLQK YKSLCM

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Macromolecule #7: Mediator of RNA polymerase II transcription subunit 10

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 10
type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mouse (mice)
Molecular weightTheoretical: 15.951878 KDa
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)IDKCRQ QLHDITVPLE VFEYIDQGRN PQLYTKECLE RALAKNEQVK GKID TMKKF KSLLIQELSK VFPEDMAKYR SIRGEDHPPS

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Macromolecule #8: Mediator of RNA polymerase II transcription subunit 11

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 11
type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mouse (mice)
Molecular weightTheoretical: 12.166723 KDa
SequenceString:
NERLRALEDI EREIGAILQN AGTAILELSK EKTNERLLDR QAAAFTTSVQ HVEAELSAQI RYLTQVATGQ PHEGSSYSSR KDCQMALKR VDYARLKISD VARTCEQML

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Macromolecule #9: Mediator of RNA polymerase II transcription subunit 14

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 14
type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mouse (mice)
Molecular weightTheoretical: 168.719531 KDa
SequenceString: MAPVQLDNHQ LIPPGGGGGS SGGGGSSSGS ASAPAPPPPA AAVAAAAAAA ASPGYRLSTL IEFLLHRAYS ELMVLTDLLP RKSDVERKI EIVQFASRTR QLFVRLLALV KWANDAGKVE KCAMISSFLD QQAILFVDTA DRLASLARDA LVHARLPSFA I PYAIDVLT ...String:
MAPVQLDNHQ LIPPGGGGGS SGGGGSSSGS ASAPAPPPPA AAVAAAAAAA ASPGYRLSTL IEFLLHRAYS ELMVLTDLLP RKSDVERKI EIVQFASRTR QLFVRLLALV KWANDAGKVE KCAMISSFLD QQAILFVDTA DRLASLARDA LVHARLPSFA I PYAIDVLT TGSYPRLPTC IRDKIIPPDP ITKIEKQATL HQLNQILRHR LVTTDLPPQL ANLTVANGRV KFRVEGEFEA TL TVMGDDP EVPWRLLKLE ILVEDKETGD GRALVHSMQI DFIHQLVQSR LFADEKPLQD MYNCLHCFCL SLQLEVLHSQ TLM LIRERW GDLVQVERYH AGKSLSLSVW NQQVLGRKTG TASVHKVTIK IDENDVSKPL QIFHDPPLPA SDSKLVERAM KIDH LSIEK LLIDSVHARA HQRLQELKAI LRSFNANESS SIETALPALI VPILEPCGNS ECLHIFVDLH SGMFQLMLYG LDPAT LEDM EKSLNDDMKR IIPWIQQLKF WLGQQRCKQS IKHLPTITTE TLQLANYSTH PIGSLSKNKL FIKLTRLPQY YIVVEM LEV PNKPTQLSYN YYFMSVSTAD REDSPVMALL LQQFKDNIQD LMSYTKTGKQ TRTGTKHKLS DDPCPIDSKK AKRSGEM CA FNKVLAHFVA MCDTNMPFVG LRLELSNLEI PHQGVQVEGD GFNHAIRLLK IPPCKGISEE TQKALDRSLL DCTFRLQG R NNRTWVAELV FANCPLNGTS TREQGPSRHV YLTYENLLSE PVGGRKVVEM FLNDWSSIAR LYECVLEFAR SLPEIPAHL NIFSEVRVYN YRKLILCYGT TKGSSISIQW NSIHQKFHIA LGTVGPNSGC SNCHNTILHQ LQEMFNKTPN VVQLLQVLFD TQAPLNAIN KLPTVPMLGL TQRTNTAYQC FSILPQSSTH IRLAFRNMYC IDIYCRSRGV VAIRDGAYSL FDNSKLVEGF Y PAPGLKTF LNMFVDSNQD ARRRSVNEDD NPPSPIGGDM MDSLISQLQP PQQQPFPKQP GTSGAYPLTS PPTSYHSTVN QS PSMMHTQ SPGNLHAASS PSGALRAPSP ASFVPTPPPS SHGISIGPGA SFASPHGTLD PSSPYTMVSP SGRAGNWPGS PQV SGPSPA TRLPGMSPAN PSLHSPVPDV SHSPRAGTSS QTMPTNMPPP RKLPQRSWAA SIPTILTHSA LNILLLPSPT PGLV PGLAG SYLCSPLERF LGSVIMRRHL QRIIQQETLQ LINSNEPGVI MFKTDALKCR VALSPKTNQT LQLKVTPENA GQWKP DELQ VLEKFFETRV AGPPFKANTL IAFTKLLGAP THILRDCVHI MKLELFPDQA TQLKWNVQFC LTIPPSAPPI APPGTP AVV LKSKMLFFLQ LTQKTSVPPQ EPVSIIVPII YDMASGTTQQ ADIPRQQNSS VAAPMMVSNI LKRFAEMNPP RQGECTI FA AVRDLMANLT LPPGGRP(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)

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Macromolecule #10: Mediator of RNA polymerase II transcription subunit 15

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 15
type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mouse (mice)
Molecular weightTheoretical: 18.904717 KDa
SequenceString:
QPLLDAVLAN IRSPVFNHSL YRTFVPAMMA IHGPPIVSPV VCSRKRRFEE DERQSIPNVL QGEVARLDPK FLVNLDPSHC SNNGTVHLI CKLDDKDLPS VPPLELSVPA DYPAQSPMWI DRQWQYDANP FLQSVHRCMT SRLLQLPDKH SVTALLNTWA Q SIHQACL

+
Macromolecule #11: Mediator of RNA polymerase II transcription subunit 16

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 16
type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mouse (mice)
Molecular weightTheoretical: 91.890484 KDa
SequenceString: MDLAYVCEWE KWAKSTYCPS LPLACAWSCR NLIAFTTDLR NDDQDLTHMI HILDTEHPWE VHSVSSGHSE AITCLEWDQS GSRLLSADA DGQIKCWSMA DHLANSWESS VGSQVEGDPI VALSWLHNGV KLALHVEKSG ASSFGEKFSR VKFSPSLTLF G GKPMEGWI ...String:
MDLAYVCEWE KWAKSTYCPS LPLACAWSCR NLIAFTTDLR NDDQDLTHMI HILDTEHPWE VHSVSSGHSE AITCLEWDQS GSRLLSADA DGQIKCWSMA DHLANSWESS VGSQVEGDPI VALSWLHNGV KLALHVEKSG ASSFGEKFSR VKFSPSLTLF G GKPMEGWI AVTVSGLVTV SLLKPSGQVL TSTESLCRLR GRVALADIAF TGGGNIVVAA ADGSSASPVK FYKVCVSVVS EK CRIDTEI LPSLFMRCTT DPNRKDRFPA ITHLKFLARD MSEQVLLCAS SQTSSLVECW SLRKEGLPVN NIFQQISPVV GDK QPMILK WRILSATNDL DRVSAVALPK LPISLTNTDL KVASDTQFYP GLGLALAFQD GSVHMVHRLS LQTMAVFYSS APRS LDEPA LKRPRTTCPA VHFKAMQLSW TSLALVGIDN HGKLSMLRIS PSLGHPLEPK LALQHLLFLL EYCMVTGYDW WDILL HVQP GMVQSLVERL HEEYTRQKPA LQQVLSTRIL AMKASLCKLS PCTVARVCDY HTKLFLMAIT STLKSLLRPH FLNTPD KSP GDRLAEICAK ITDVDIDKVM INLKTEEFVL DMNTLQALQQ LLQWVGDFVL YLLVSLPNQG SPLRPGHSFL RDGTSLG ML RELMVVIRIW GLLKPSCLPV YTATSDTQDS MSLLFRLLTK LWICCRDEGA ASEPDEGLVD ECCLLPSQLL VPNLDWLP A SDGLVSRLQP KQPLRLRFGR APTLPSSTST LQLDGLTRAP GQPKIDHLRR LHLGAYPTEE CKACTRCGCV TMLKSPNKT TAVTQWEQRW IKNCLCGGLW RRVPLSCS

+
Macromolecule #12: Mediator of RNA polymerase II transcription subunit 17

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 17
type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mouse (mice)
Molecular weightTheoretical: 70.66932 KDa
SequenceString: CEKQVQEVGL DGTETYLQPL SMSQNLARLA QRIDFSQGSG SEEEEAAGPD GDAPDWGGAG ADQDDEEGLV KFQPSLWPWD SVRNNLRSA LTEMCVLYDV LSIVRDKKFM TLDPVSQDAL PPKQSPQTLQ LISKKKSLAG AAQILLKGAE RLTKSVAENQ E NKLQRDFN ...String:
CEKQVQEVGL DGTETYLQPL SMSQNLARLA QRIDFSQGSG SEEEEAAGPD GDAPDWGGAG ADQDDEEGLV KFQPSLWPWD SVRNNLRSA LTEMCVLYDV LSIVRDKKFM TLDPVSQDAL PPKQSPQTLQ LISKKKSLAG AAQILLKGAE RLTKSVAENQ E NKLQRDFN SELLRLRQHW KLRKVGDKIL GDLSYRSAGS LFPHHGTFEV IKNTDIDLDK KIPEDYCPLD VQIPSDLEGS AY IKVSIQK QAPDIGDLGT VNLFKRPLPK SKPGSPHWQT KLEAAQNVLL CKEIFAQLSR EAVQIKSQIP HIVVKNQIIS QPF PSLQLS ISLCHSSDDK KSQKCAAEKP GQEDHLYVLE HNLHLLIREF HKQTLSSIVM PHPASAPFGH KRMRLSGPQA FDKN EINSI QSTEGLLEKI IKQAKHIFLR SRTAATIDSL ASRIEDPQIQ AHWSNINDVY ESSVKVLITS QGYEQICKSI QLQLN IGVE QVRVVHRDGR VIMLSHQEQE LQDFLLSQMS QHQVHAVQQL AKVMGWQVLS FSNHVGLGPI ESIGNASAIT VASPSG DYA ISVRNGPESG SKIMVQFPRN QCKDLPKSDV LQDSKWSHLR GPFKEVQWNK MEGRNFVYKM ELLMSALS

+
Macromolecule #13: Mediator of RNA polymerase II transcription subunit 18

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 18
type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mouse (mice)
Molecular weightTheoretical: 21.43385 KDa
SequenceString:
NMMEYLLQGS VLDHSLESLI HRLRGLCDNM EPETFLDHEM VFLLKGQQAS PFVLRARRSM DRAGAPWHLR YLGQPEMGDK NRHALVRNC VDIATSENLT DFLMEMGFRM DHEFVAKGHL FRKGIMKVVV YKIFRILVPG NTDSTEALSL SYLVELSVVA P AGQDMVSD DMRNFAEQLK PLVHLEKID

+
Macromolecule #14: Mediator of RNA polymerase II transcription subunit 19

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 19
type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mouse (mice)
Molecular weightTheoretical: 24.684615 KDa
SequenceString: MENFTALFGA QTDPPPPPSA LGFGPGKPPP PPPPPPGGGP GAAPPSTATS APAGADKSTA GSGPFYLMRE LPGSTEL (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) ...String:
MENFTALFGA QTDPPPPPSA LGFGPGKPPP PPPPPPGGGP GAAPPSTATS APAGADKSTA GSGPFYLMRE LPGSTEL (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)L GGSFNPITGT MLSGFRLHTG PLPEQCRLM HIQPPKKKNK HKHKQSRTQD PVPPETPSDS DHKKKKKKKE EDPERKRKKK EKKKKKNRHS PDHPGMGSSQ ASSSSSLR

+
Macromolecule #15: Mediator of RNA polymerase II transcription subunit 20

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 20
type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mouse (mice)
Molecular weightTheoretical: 21.660906 KDa
SequenceString: VTCVSQMPVA EGKSLQQTVE LLTKKLEMLG AEKQGTFCVD CETYHTAAST LGSQGQAGKL MYVMHNSEYP LSCFALFENG PCLIADTNF DVLMVKLKGF FQSAKASKIE TRGTRYQYCD FLVKVGTVTM GPSARGISVE VEYGPCVVAS DCWSLLLEFL Q SFLGSHAP ...String:
VTCVSQMPVA EGKSLQQTVE LLTKKLEMLG AEKQGTFCVD CETYHTAAST LGSQGQAGKL MYVMHNSEYP LSCFALFENG PCLIADTNF DVLMVKLKGF FQSAKASKIE TRGTRYQYCD FLVKVGTVTM GPSARGISVE VEYGPCVVAS DCWSLLLEFL Q SFLGSHAP GAPTVFGNRH DAVYGPADTM IQYMELFNKI

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Macromolecule #16: Mediator of RNA polymerase II transcription subunit 21

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 21
type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mouse (mice)
Molecular weightTheoretical: 13.619062 KDa
SequenceString:
DRLTQLQDAV NSLADQFCNA IGVLQQCGPP ASFSNIQTAI NKDQPANPTE EYAQLFAALI ARTAKDIDVL IDSLPSEEST AALQAASLY KLEEENHEAA TCLEDVVYRG DMLLEKIQSA LADIAQS

+
Macromolecule #17: Mediator of RNA polymerase II transcription subunit 22

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 22
type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mouse (mice)
Molecular weightTheoretical: 15.284022 KDa
SequenceString:
QSKETLLQSY NKRLKDDIKS IMDNFTEIIK TAKIEDETQV SRATQGEQDN YEMHVRAANI VRAGESLMKL VSDLKQFLIL NDFPSVNEA IDQRNQQLRA LQEECDRKLI TLRDEVSIDL YELEEEYYSS SS

+
Macromolecule #18: Mediator of RNA polymerase II transcription subunit 23

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 23
type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mouse (mice)
Molecular weightTheoretical: 156.265656 KDa
SequenceString: METQLQSIFE EVVKTEIIEE AFPGMFMDTP EDEKTKLISC LAAFRQFWSG LSQESHEQCV QWIVKFIHGQ HSPKRISFLY DCLAMAVET GLLPPRMVCE SLINSDSLEW ERTQLWALTF KLVRKIIGGV DYKGVRDLLK AILEKILTIP NTVSSAVVQQ L LAAREVIA ...String:
METQLQSIFE EVVKTEIIEE AFPGMFMDTP EDEKTKLISC LAAFRQFWSG LSQESHEQCV QWIVKFIHGQ HSPKRISFLY DCLAMAVET GLLPPRMVCE SLINSDSLEW ERTQLWALTF KLVRKIIGGV DYKGVRDLLK AILEKILTIP NTVSSAVVQQ L LAAREVIA YILERNACLL PAYFAVTEIR KLYPEGKLPH WLLGNLVSDF VDTFRPTARI NSICGRCSLL PVVNNSGAIC NS WKLDPAT LRFPLKGLLP YDKDLFEPQT ALLRYVLEQP YSRDMVCNML GLNKQHKQRC PVLEDQLVDL VVYAMERSET EEK FDDGGT SQLLWQHLSS QLIFFVLFQF ASFPHMVLSL HQKLAGRGLI KGRDHLMWVL LQFISGSIQK NALADFLPVM KLFD LLYPE KECIPVPDIN KPQSTHAFAM TCIWIHLNRK AQNGDSTLQI PIPHSLKLHH EFLQQSLRNK SLQMNDYKIA LLCNA YSTN SECFTLPMGA LVETIYGNGI MRVPLPGTSC LASASVTPLP MNLLDSLTVH AKMSLIHSIA TRVIKLAHTK SSVALA PAL VETYSRLLVY MEIESLGIKG FISQLLPTVF KSHAWGILHT LLEMFSHRMH HIQPHYRVQL LSHLHTLAAV AQTNQNQ LH LCVESTALRL ITALGSSEVQ PQFTRFLNDP KTVLSAESEE LNRALILTLA RATHVTDFFT GSDSIQGTWC KDILQTIM N FTPHNWASHT LSCFPAPLQA FFKQNNVPQE SRFNLKKNVE EEYRKWKSMT DENEIITQFS VQGFPPLFLC LLWKMLLET DHISQIGYKV LERIGARALV AHVRTFADFL VYEFSTSAGG QQLNKCIEIL NDMVWKYNIV TLDRLILCLA MRSHEGNEAQ VCYFIIQLL LLKPNDFRNR VSDFVKENSP EHWLQSDWHT KHMSYHKKYP EKLYFEGLAE QVDPPVPIQS PYLPIYFGNV C LRFLPVFD IVIHRFLELL PVSKSLETLL DHLGGLYKFH DRPVTYLYNT LHYYEMCLRN RDHLKRKLVH AIIGSLKDNR PQ GWCLSDT YLKHAMNARE DNPWVPEDSY YCKLIGRLVD TMAGKSPGPF PNCDWRFNEF PNPAAHALHV TCVELMALAV PGK DVGNAL LNVVLKSQPL VPRENITAWM NAIGLIITAL PEPYWIVLHD RIVNVISSSS LTSETEWVGY PFRLFDFTAC HQSY SEMSC SYTLALAHAV WHHSSIGQLS LIPKFLTEAL LPVVKTEFQL LYVYHLVGPF LQRFQQERTR CMIEIGVAFY DMLLN VDQC STHLNYMDPI CDFLYHMKYM FTGDSVKEQV EKIICNLKPA LKLRLRFITH ISKMEPAVPP QALNSGSPAP QSNQVP ASL PVTQ

+
Macromolecule #19: Mediator of RNA polymerase II transcription subunit 24

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 24
type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mouse (mice)
Molecular weightTheoretical: 109.563117 KDa
SequenceString: VNLKQAILQA WKERWSDYQW AINMKKFFPK GATWDILNLA EALLEQAMIG PSPNPLILSY LKYAISSQMV SCSSVLTAIS KFDDFSRDL CVQALLDIMD MFCDRLSCHG KAEECIGLCR ALLSALHWLL RCTAASAERL QEGLEAGTPA PGEKQLALCL Q CLEKTLSS ...String:
VNLKQAILQA WKERWSDYQW AINMKKFFPK GATWDILNLA EALLEQAMIG PSPNPLILSY LKYAISSQMV SCSSVLTAIS KFDDFSRDL CVQALLDIMD MFCDRLSCHG KAEECIGLCR ALLSALHWLL RCTAASAERL QEGLEAGTPA PGEKQLALCL Q CLEKTLSS TKNRALLHIA KLEEASSWTA IEHSLLKLGE ILANLSNPQL RSQAERCGTL IRSIPSMLSV HSEQLHKTGF PT IHALILL EGTMNLTGEM QPLVEQLMMV KRMQHIPTPL FVLEIWKACF VGLIESPEGT QELKWTAFTY LKIPQVLVKL KKY FHGEKD FTEDVNCAFE FLLKLTPLLD KADQRCNCDC TNFLLQECNK QGLLSEVNFA SLVGKRTADR DPQLKSSENA NIQP NPGLI LRAEPTVTNI LKTMDADHSK SPEGLLGVLG HMLSGKSLDL LLAAAAATGK LKSFARKFIN LNEFTTHGSG ESTKT ASVR ALLFDISFLM LCHVAQTYGS EVILSESSSG EEVPFFETWM QTCMPEEGKI LNPDHPCFRP DSTKVESLVA LLNNSS EMK LVQMKWHEAC LSISAAILEI LNAWENGVLA FESIQKITDN IKGKVCSLAV CAVAWLVAHV RMLGLDEREK SLQMIRQ LA GPLYSENTLQ FYNERVVIMN SILEHMCADV LQQTATQIKF PSTGVDTMPY WNLLPPKRPI KEVLTDIFAK VLEKGWVD S RSIHILDTLL HMGGVYWFCN NLIKELLKET RKEHTLRAVQ LLYSIFCLDM QQVTLVLLGH ILPGLLTDSS KWHSLMDPP GTALAKLAVW CALSSYSSHK GQASSRQKKR HREDIEDYVS LFPVEDMQPS KLMRLLSSSD DDANILSSPT DRSMNSSLSA SQLHTVNMR DPLNRVLANL FLLISSILGS RTAGPHTQFV QWFMEECVGC LEQDSRGSIL QFMPFTTVSE LVKVSAMSSP K VVLAITDL SLPLGRQVAA KAIA

+
Macromolecule #20: Mediator of RNA polymerase II transcription subunit 25

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 25
type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mouse (mice)
Molecular weightTheoretical: 22.032941 KDa
SequenceString: VADVVFVIEG TANLGPYFEE LRKHYLLPAI EYFNGGPPAE TDFGGDYGGT QYSLVVFNTV DCAPESYVQC HAPTSSAYEF VTWLDGIKF MGGGGESCSL IAEGLSTALQ LFDDFKKMRE QIGQTHRVCL LICNSPPYLL PAVESTTYSG CTTESLVQKI G ERGIHFSI ...String:
VADVVFVIEG TANLGPYFEE LRKHYLLPAI EYFNGGPPAE TDFGGDYGGT QYSLVVFNTV DCAPESYVQC HAPTSSAYEF VTWLDGIKF MGGGGESCSL IAEGLSTALQ LFDDFKKMRE QIGQTHRVCL LICNSPPYLL PAVESTTYSG CTTESLVQKI G ERGIHFSI VSPRKLPALR LLFEKAAPPA LLEPLQQPAD VSQD

+
Macromolecule #21: Mediator of RNA polymerase II transcription subunit 27

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 27
type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mouse (mice)
Molecular weightTheoretical: 33.737773 KDa
SequenceString: GVNLEAFSQA ISAIQALRSS VSRVFDCLKD GMRNKETLEG REKAFIANFQ DNLHSVNRDL NELERLSNLV GKPSENHPLH NSGLLSLDP VQDKTPLYSQ LLQAYKWSNK LQYHAGLASG LLNQQSLKRS ANQMGVSAKR RPKAQPTTLV LPPQYVDDVI S RIDRMFPE ...String:
GVNLEAFSQA ISAIQALRSS VSRVFDCLKD GMRNKETLEG REKAFIANFQ DNLHSVNRDL NELERLSNLV GKPSENHPLH NSGLLSLDP VQDKTPLYSQ LLQAYKWSNK LQYHAGLASG LLNQQSLKRS ANQMGVSAKR RPKAQPTTLV LPPQYVDDVI S RIDRMFPE MSIHLSRPNG TSAMLLVTLG KVLKVIVVMR SLFIDRTIVK GYNESVYTED GKLDIWSKSS YQVFQKVTDH AT TALLHYQ LPQMPDVVVR SFMTWLRSYI KLFQAPCQRC GKFLQDGLPP TWRDFRTLEA

+
Macromolecule #22: Mediator of RNA polymerase II transcription subunit 28

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 28
type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mouse (mice)
Molecular weightTheoretical: 13.531168 KDa
SequenceString:
AAPGAPRPSN STLVDELESS FEACFASLVS QDYVNGTDQE EIRTGVDQCI QKFLDIARQT ECFFLQKRLQ LSVQKPDQVI KEDVSELRS ELQRKDALVQ KHLTKLRHWQ QVLEDINVQ

+
Macromolecule #23: Mediator of RNA polymerase II transcription subunit 29

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 29
type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mouse (mice)
Molecular weightTheoretical: 14.965154 KDa
SequenceString:
DFDPVQRYKM LIPQLKESLQ TLMKVAAQNL IQNTNIDNGQ KSSDAPLQRF DKCLEEFYAL CDQLELCLRL AHECLSQSCD SAKHSPTLV PTATKPDAVQ PDSLPYPQYL AVIKAQITCA KDIHTALLDC ANKVT

+
Macromolecule #24: Mediator of RNA polymerase II transcription subunit 30

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 30
type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mouse (mice)
Molecular weightTheoretical: 17.873512 KDa
SequenceString:
EVNTATLCRI GQETVQDIVY RTMEIFQLLR NMQLPNGVTY HTGTYQDRLT KLQDHLRQLS ILFRKLRLVY DKCNENCGGM DPIPVEQLI PYVDEDGSKN DDRAGPPRFA SEERREIVEV NKKLKQKNQQ LKQIMDQLRN LIWDINAMLA MRN

+
Macromolecule #25: Mediator of RNA polymerase II transcription subunit 31

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 31
type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mouse (mice)
Molecular weightTheoretical: 11.54537 KDa
SequenceString:
GNRLRFQLEL EFVQCLANPN YLNFLAQRGY FKDKAFVNYL KYLLYWKEPE YAKYLKYPQC LHMLELLQYE HFRKELVNAQ CAKFIDEQQ ILHW

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration0.1 mg/mL
BufferpH: 7.9
Sugar embeddingMaterial: ice
GridModel: PELCO Ultrathin Carbon with Lacey Carbon / Material: COPPER / Mesh: 400 / Support film - Material: CARBON / Support film - topology: CONTINUOUS / Support film - Film thickness: 30.0 nm / Pretreatment - Type: PLASMA CLEANING
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: HOMEMADE PLUNGER

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Electron microscopy

MicroscopeFEI TALOS ARCTICA
Electron beamAcceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 50.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Cs: 2.7 mm
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 22.5 e/Å2
Experimental equipment
Model: Talos Arctica / Image courtesy: FEI Company

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Image processing

Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
Final reconstructionResolution.type: BY AUTHOR / Resolution: 4.02 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 217557
FSC plot (resolution estimation)

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