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Open data
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Basic information
Entry | Database: EMDB / ID: EMD-30135 | |||||||||
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Title | Mature bacteriorphage t7 tail adaptor protein gp11 | |||||||||
![]() | GP11 mature viron particle | |||||||||
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![]() | Phage / portal / mature / complex / STRUCTURAL PROTEIN | |||||||||
Function / homology | Tail tubular protein Gp11 / Tail tubular protein / virus tail, tube / symbiont genome ejection through host cell envelope, short tail mechanism / Tail tubular protein gp11![]() | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.8 Å | |||||||||
![]() | Chen WY / Xiao H | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Structural changes of a bacteriophage upon DNA packaging and maturation. Authors: Wenyuan Chen / Hao Xiao / Xurong Wang / Shuanglin Song / Zhen Han / Xiaowu Li / Fan Yang / Li Wang / Jingdong Song / Hongrong Liu / Lingpeng Cheng / ![]() | |||||||||
History |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 1.2 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 9.3 KB 9.3 KB | Display Display | ![]() |
Images | ![]() | 105.1 KB | ||
Filedesc metadata | ![]() | 4.7 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7boxMC ![]() 7bouC ![]() 7boyC ![]() 7bozC ![]() 7bp0C M: atomic model generated by this map C: citing same article ( |
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Similar structure data |
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Annotation | GP11 mature viron particle | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.27 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
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Sample components
-Entire : Mature bacteriorphage t7 tail adaptor protein gp11
Entire | Name: Mature bacteriorphage t7 tail adaptor protein gp11 |
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Components |
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-Supramolecule #1: Mature bacteriorphage t7 tail adaptor protein gp11
Supramolecule | Name: Mature bacteriorphage t7 tail adaptor protein gp11 / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all |
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Source (natural) | Organism: ![]() ![]() |
-Macromolecule #1: Tail adaptor protein gp11
Macromolecule | Name: Tail adaptor protein gp11 / type: protein_or_peptide / ID: 1 / Number of copies: 12 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 22.30765 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: MRSYDMNVET AAELSAVNDI LASIGEPPVS TLEGDANADA ANARRILNKI NRQIQSRGWT FNIEEGITLL PDVYSNLIVY SDDYLSLMS TSGQSIYVNR GGYVYDRTSQ SDRFDSGITV NIIRLRDYDE MPECFRYWIV TKASRQFNNR FFGAPEVEGV L QEEEDEAR ...String: MRSYDMNVET AAELSAVNDI LASIGEPPVS TLEGDANADA ANARRILNKI NRQIQSRGWT FNIEEGITLL PDVYSNLIVY SDDYLSLMS TSGQSIYVNR GGYVYDRTSQ SDRFDSGITV NIIRLRDYDE MPECFRYWIV TKASRQFNNR FFGAPEVEGV L QEEEDEAR RLCMEYEMDY GGYNMLDGDA FTSGLLTR UniProtKB: Tail tubular protein gp11 |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 7 |
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Vitrification | Cryogen name: NITROGEN |
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Electron microscopy
Microscope | FEI TECNAI ARCTICA |
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Image recording | Film or detector model: FEI FALCON II (4k x 4k) / Average electron dose: 24.0 e/Å2 |
Electron beam | Acceleration voltage: 200 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD |
Experimental equipment | ![]() Model: Talos Arctica / Image courtesy: FEI Company |
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Image processing
Startup model | Type of model: NONE |
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Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 3.8 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 55212 |
Initial angle assignment | Type: COMMON LINE |
Final angle assignment | Type: PROJECTION MATCHING |