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- PDB-4pph: Crystal structure of conglutin gamma, a unique basic 7S globulin ... -

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Basic information

Entry
Database: PDB / ID: 4pph
TitleCrystal structure of conglutin gamma, a unique basic 7S globulin from lupine seeds
ComponentsConglutin gamma
KeywordsPLANT PROTEIN / lupine cotyledons / non-storage role / 7S basic globulin / glycoside-hydrolase-inhibitor-like protein / apigenin glycosides / N-linked glycosylation / insulin
Function / homology
Function and homology information


aspartic-type endopeptidase activity / proteolysis / extracellular region
Similarity search - Function
Xylanase inhibitor, C-terminal / Xylanase inhibitor I-like / Xylanase inhibitor C-terminal / Xylanase inhibitor, N-terminal / Xylanase inhibitor N-terminal / Aspartic peptidase A1 family / Peptidase family A1 domain / Peptidase family A1 domain profile. / Cathepsin D, subunit A; domain 1 / Acid Proteases ...Xylanase inhibitor, C-terminal / Xylanase inhibitor I-like / Xylanase inhibitor C-terminal / Xylanase inhibitor, N-terminal / Xylanase inhibitor N-terminal / Aspartic peptidase A1 family / Peptidase family A1 domain / Peptidase family A1 domain profile. / Cathepsin D, subunit A; domain 1 / Acid Proteases / Aspartic peptidase domain superfamily / Beta Barrel / Mainly Beta
Similarity search - Domain/homology
Biological speciesLupinus angustifolius (narrow-leaved blue lupine)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.009 Å
AuthorsCzubinski, J. / Barciszewski, J. / Gilski, M. / Lampart-Szczapa, E. / Jaskolski, M.
Citation
Journal: Acta Crystallogr.,Sect.D / Year: 2015
Title: Structure of gamma-conglutin: insight into the quaternary structure of 7S basic globulins from legumes.
Authors: Czubinski, J. / Barciszewski, J. / Gilski, M. / Szpotkowski, K. / Debski, J. / Lampart-Szczapa, E. / Jaskolski, M.
#1: Journal: Food Chem / Year: 2014
Title: Characterisation of different digestion susceptibility of lupin seed globulins.
Authors: Czubinski, J. / Dwiecki, K. / Siger, A. / Neunert, G. / Lampart-Szczapa, E.
#2: Journal: J.Agric.Food Chem. / Year: 2012
Title: Release of flavonoids from lupin globulin proteins during digestion in a model system.
Authors: Czubinski, J. / Dwiecki, K. / Siger, A. / Kachlicki, P. / Neunert, G. / Lampart-Szczapa, E. / Nogala-Kalucka, M.
#3: Journal: Febs J. / Year: 2011
Title: Crystal structure of basic 7S globulin, a xyloglucan-specific endo-beta-1,4-glucanase inhibitor protein-like protein from soybean lacking inhibitory activity against endo-beta-glucanase.
Authors: Yoshizawa, T. / Shimizu, T. / Yamabe, M. / Taichi, M. / Nishiuchi, Y. / Shichijo, N. / Unzai, S. / Hirano, H. / Sato, M. / Hashimoto, H.
#4: Journal: Biochem.Biophys.Res.Commun. / Year: 2013
Title: Internalisation and multiple phosphorylation of gamma-Conglutin, the lupin seed glycaemia-lowering protein, in HepG2 cells.
Authors: Capraro, J. / Magni, C. / Faoro, F. / Maffi, D. / Scarafoni, A. / Tedeschi, G. / Maffioli, E. / Parolari, A. / Manzoni, C. / Lovati, M.R. / Duranti, M.
#5: Journal: Br. J. Nutr. / Year: 2012
Title: Lupin seed gamma-conglutin lowers blood glucose in hyperglycaemic rats and increases glucose consumption of HepG2 cells.
Authors: Lovati, M.R. / Manzoni, C. / Castiglioni, S. / Parolari, A. / Magni, C. / Duranti, M.
History
DepositionFeb 27, 2014Deposition site: RCSB / Processing site: RCSB
Revision 1.0Feb 11, 2015Provider: repository / Type: Initial release
Revision 1.1Feb 25, 2015Group: Database references
Revision 1.2Nov 22, 2017Group: Advisory / Database references / Refinement description
Category: citation / pdbx_unobs_or_zero_occ_atoms ...citation / pdbx_unobs_or_zero_occ_atoms / pdbx_unobs_or_zero_occ_residues / software
Item: _citation.journal_abbrev / _software.name
Revision 2.0Jul 29, 2020Group: Atomic model / Data collection ...Atomic model / Data collection / Database references / Derived calculations / Structure summary
Category: atom_site / chem_comp ...atom_site / chem_comp / citation / entity / pdbx_branch_scheme / pdbx_chem_comp_identifier / pdbx_entity_branch / pdbx_entity_branch_descriptor / pdbx_entity_branch_link / pdbx_entity_branch_list / pdbx_entity_nonpoly / pdbx_nonpoly_scheme / pdbx_struct_assembly_gen / struct_asym / struct_conn / struct_site / struct_site_gen
Item: _atom_site.B_iso_or_equiv / _atom_site.Cartn_x ..._atom_site.B_iso_or_equiv / _atom_site.Cartn_x / _atom_site.Cartn_y / _atom_site.Cartn_z / _atom_site.auth_asym_id / _atom_site.auth_atom_id / _atom_site.auth_comp_id / _atom_site.auth_seq_id / _atom_site.label_asym_id / _atom_site.label_atom_id / _atom_site.label_comp_id / _atom_site.label_entity_id / _atom_site.type_symbol / _chem_comp.name / _chem_comp.type / _citation.country / _citation.journal_id_ISSN / _pdbx_struct_assembly_gen.asym_id_list / _struct_conn.pdbx_dist_value / _struct_conn.pdbx_leaving_atom_flag / _struct_conn.pdbx_role / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_comp_id
Description: Carbohydrate remediation / Provider: repository / Type: Remediation
Revision 2.1Sep 20, 2023Group: Advisory / Data collection ...Advisory / Data collection / Database references / Refinement description / Structure summary
Category: chem_comp / chem_comp_atom ...chem_comp / chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / pdbx_unobs_or_zero_occ_atoms / pdbx_unobs_or_zero_occ_residues
Item: _chem_comp.pdbx_synonyms / _database_2.pdbx_DOI / _database_2.pdbx_database_accession
Revision 2.2Nov 20, 2024Group: Structure summary / Category: pdbx_entry_details / pdbx_modification_feature

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Conglutin gamma
B: Conglutin gamma
C: Conglutin gamma
D: Conglutin gamma
E: Conglutin gamma
F: Conglutin gamma
hetero molecules


Theoretical massNumber of molelcules
Total (without water)276,11143
Polymers272,4666
Non-polymers3,64537
Water14,304794
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)121.990, 121.990, 188.490
Angle α, β, γ (deg.)90.00, 90.00, 120.00
Int Tables number144
Space group name H-MP31

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Components

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Protein , 1 types, 6 molecules ABCDEF

#1: Protein
Conglutin gamma / Conglutin gamma 1


Mass: 45411.059 Da / Num. of mol.: 6
Fragment: Lupinus angustifolius Conglutin gamma,UNP residues 33-449
Source method: isolated from a natural source
Source: (natural) Lupinus angustifolius (narrow-leaved blue lupine)
References: UniProt: Q42369

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Sugars , 3 types, 5 molecules

#2: Polysaccharide alpha-L-fucopyranose-(1-3)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)]2-acetamido-2-deoxy-beta- ...alpha-L-fucopyranose-(1-3)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)]2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 570.542 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
LFucpa1-3[DGlcpNAcb1-4]DGlcpNAcb1-Glycam Condensed SequenceGMML 1.0
WURCS=2.0/2,3,2/[a2122h-1b_1-5_2*NCC/3=O][a1221m-1a_1-5]/1-2-1/a3-b1_a4-c1WURCSPDB2Glycan 1.1.0
[]{[(4+1)][b-D-GlcpNAc]{[(3+1)][a-L-Fucp]{}[(4+1)][b-D-GlcpNAc]{}}}LINUCSPDB-CARE
#3: Polysaccharide 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 424.401 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DGlcpNAcb1-4DGlcpNAcb1-Glycam Condensed SequenceGMML 1.0
WURCS=2.0/1,2,1/[a2122h-1b_1-5_2*NCC/3=O]/1-1/a4-b1WURCSPDB2Glycan 1.1.0
[]{[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{}}}LINUCSPDB-CARE
#5: Sugar ChemComp-NAG / 2-acetamido-2-deoxy-beta-D-glucopyranose / N-acetyl-beta-D-glucosamine / 2-acetamido-2-deoxy-beta-D-glucose / 2-acetamido-2-deoxy-D-glucose / 2-acetamido-2-deoxy-glucose / N-ACETYL-D-GLUCOSAMINE


Type: D-saccharide, beta linking / Mass: 221.208 Da / Num. of mol.: 3
Source method: isolated from a genetically manipulated source
Formula: C8H15NO6
IdentifierTypeProgram
DGlcpNAcbCONDENSED IUPAC CARBOHYDRATE SYMBOLGMML 1.0
N-acetyl-b-D-glucopyranosamineCOMMON NAMEGMML 1.0
b-D-GlcpNAcIUPAC CARBOHYDRATE SYMBOLPDB-CARE 1.0
GlcNAcSNFG CARBOHYDRATE SYMBOLGMML 1.0

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Non-polymers , 2 types, 826 molecules

#4: Chemical...
ChemComp-EDO / 1,2-ETHANEDIOL / ETHYLENE GLYCOL


Mass: 62.068 Da / Num. of mol.: 32 / Source method: obtained synthetically / Formula: C2H6O2
#6: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 794 / Source method: isolated from a natural source / Formula: H2O

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Details

Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.97 Å3/Da / Density % sol: 58.61 %
Crystal growTemperature: 292 K / Method: vapor diffusion, hanging drop / pH: 7
Details: 7% PEG 6000, 0.1M HEPES pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: BESSY / Beamline: 14.2 / Wavelength: 0.91841 Å
DetectorType: RAYONIX MX-225 / Detector: CCD / Date: Jul 7, 2013
RadiationMonochromator: Double Crystal Monochromator, Si-111 crystal / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.91841 Å / Relative weight: 1
ReflectionResolution: 2→43.76 Å / Num. all: 208256 / Num. obs: 208256 / % possible obs: 99.7 % / Observed criterion σ(I): -3 / Redundancy: 3.86 % / Biso Wilson estimate: 42.37 Å2 / Rmerge(I) obs: 0.068 / Net I/σ(I): 13.22
Reflection shellResolution: 2→2.12 Å / Redundancy: 3.86 % / Rmerge(I) obs: 0.755 / Mean I/σ(I) obs: 1.98 / % possible all: 99.3

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Processing

Software
NameVersionClassification
MAR345data collection
PHASERphasing
PHENIX(phenix.refine: 1.8.3_1479)refinement
XDSdata reduction
XSCALEdata scaling
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: PDB ENTRY 3AUP, chain A

3aup


Resolution: 2.009→43.764 Å / σ(F): 1.97 / Phase error: 27.18 / Stereochemistry target values: TWIN_LSQ_F / Details: HYDROGEN ATOMS WERE ADDED AT RIDING POSITIONS
RfactorNum. reflection% reflection
Rfree0.174 999 0.48 %
Rwork0.1446 --
obs0.1457 208256 99.54 %
all-208256 -
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 45.18 Å2
Refinement stepCycle: LAST / Resolution: 2.009→43.764 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms17982 0 236 794 19012
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.01118741
X-RAY DIFFRACTIONf_angle_d1.42525374
X-RAY DIFFRACTIONf_dihedral_angle_d14.0716654
X-RAY DIFFRACTIONf_chiral_restr0.0612906
X-RAY DIFFRACTIONf_plane_restr0.0073294
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.0097-2.11570.25041420.243429380X-RAY DIFFRACTION99
2.1157-2.24820.25161430.219629717X-RAY DIFFRACTION99
2.2482-2.42180.22561430.194529648X-RAY DIFFRACTION99
2.4218-2.66550.2241420.178729676X-RAY DIFFRACTION99
2.6655-3.05110.18041440.164629705X-RAY DIFFRACTION99
3.0511-3.84370.16561430.138429607X-RAY DIFFRACTION99
3.8437-43.77450.13421420.102429486X-RAY DIFFRACTION99
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
13.9139-0.9379-0.45791.6025-0.16081.1490.22870.94380.1828-0.4379-0.1732-0.05450.0291-0.0722-0.04650.3090.05070.00950.4270.04570.2044115.945675.0278-14.3344
23.2912-0.6176-0.19772.19060.19831.48860.09240.70120.2818-0.3112-0.03510.1414-0.0925-0.1554-0.05140.2460.04420.01370.34870.10150.2178110.106980.5227-9.917
36.7922-0.5108-1.53362.1641-0.3161.14990.0609-0.26970.60510.24610.0610.1176-0.14970.07-0.1140.24820.0535-0.00590.2549-0.00430.193796.125982.18014.9256
47.3472-0.13390.12971.8667-0.0731.719-0.02691.26920.2376-0.3136-0.02740.743-0.0754-0.51610.04080.27950.0395-0.06810.5635-0.00850.479977.462774.137-7.942
55.12121.3232-0.02883.40310.38841.62130.03450.49570.4868-0.162-0.00820.3907-0.1058-0.2572-0.01450.1970.07070.0240.30040.0530.289890.042282.2625-1.8552
61.8301-1.9697-1.16865.4543-0.64452.4266-0.133-0.2434-0.22720.51090.18320.19810.0683-0.0495-0.03090.21380.02050.00520.30270.01570.204772.389437.258631.3354
71.3617-1.6454-1.04536.9625-0.37041.8521-0.0481-0.3931-0.22630.59160.14890.07990.12870.2766-0.05440.19910.0141-0.06750.29330.02960.24782.877127.728827.043
82.2954-0.6771-1.75056.55070.85172.5676-0.142-0.44470.1910.43640.2283-0.74610.19880.6865-0.06620.23050.0595-0.04970.3533-0.03850.349190.046924.355220.6982
92.6082-0.5525-0.35563.11430.34021.7615-0.0035-0.2228-0.01160.20960.09860.0307-0.00010.0676-0.09370.22520.0174-0.01740.24720.02530.188174.356935.533625.6714
102.1496-0.323-0.92552.814-0.99562.90510.02310.140.1136-0.06990.14120.7205-0.0952-0.4939-0.150.18190.014-0.03350.23690.00020.369662.763546.881116.6023
112.86930.08420.43876.7903-0.69854.07270.14820.07660.60210.06940.012-0.0697-0.55220.2336-0.14420.1919-0.02260.07510.2075-0.00580.33777.561863.529515.6797
120.01910.360.1397.03891.6724.837-0.0003-0.69950.71150.8825-0.09350.1876-0.8728-0.35230.01260.5933-0.04480.13280.486-0.18740.673775.25873.674929.9195
134.90440.4060.90589.68784.11312.02110.1577-0.18590.2928-0.2442-0.28940.8696-0.5419-0.68970.10630.29250.00850.06540.25260.05310.533766.264161.34215.3646
143.311.0309-0.56044.60470.34662.85680.0485-0.30680.26990.2393-0.03790.4458-0.2805-0.0926-0.01930.19790.05630.00630.19010.02170.257368.124553.783623.3341
155.7064-1.2307-0.86733.52680.24540.62720.02380.55040.0853-0.8549-0.20690.7299-0.0401-0.24060.1540.4497-0.0236-0.21110.2749-0.02280.356365.714420.4191-6.1758
166.3194-0.49910.3952.49840.51183.43460.15490.90840.2449-0.8898-0.08360.3411-0.43750.0761-0.05210.520.0045-0.08840.30250.04030.266871.209239.7027-5.4687
171.6923-0.0148-0.28733.1104-0.12432.08650.00450.3054-0.1895-0.57970.04250.48770.1062-0.129-0.05060.3213-0.0031-0.15580.2332-0.01620.313567.842821.1363-1.9221
186.347-1.4071-0.02794.1234-0.25411.585-0.1024-0.3004-0.5360.20830.07150.42460.0899-0.10110.02680.27670.006-0.03430.1554-0.00890.214673.7127.8239.5988
197.2408-6.55610.20079.2237-1.9072.69580.16110.2247-0.2255-0.5344-0.2348-0.38060.14280.28020.05660.2703-0.01890.03770.1875-0.0150.342291.23262.5545-3.1595
203.6122-2.7017-0.25747.84922.41268.44210.32880.7038-0.8272-1.1858-0.39020.43931.2652-0.44830.03810.69210.03090.03360.4294-0.13670.627586.9071-10.1757-14.3421
215.61862.81622.51855.58742.49289.24550.1741-0.1009-0.81180.25440.0504-0.07420.2391-0.147-0.36940.31660.0558-0.0160.28890.0070.452683.9643-2.16035.0355
223.3018-1.3263-1.36413.18680.36842.71750.06880.3492-0.4323-0.3864-0.13240.38940.1764-0.20270.04320.30130.024-0.08870.1766-0.02640.341575.8443.5381-0.8034
232.89741.659-0.12414.6917-1.43991.73160.2709-0.57640.24850.9444-0.13510.1853-0.43550.0409-0.09710.4260.03650.10350.4125-0.05210.2356135.026416.283617.2816
243.05281.00930.34841.1164-0.12550.86820.0628-0.4841-0.24610.1903-0.02940.2207-0.0271-0.1155-0.02060.28550.06270.10920.30460.0780.3445122.82331.224211.7919
254.7203-0.1773-0.62781.58360.04862.1226-0.1167-0.5792-0.55740.25060.15990.34260.1347-0.3138-0.03540.2790.05050.10810.34590.11090.4522115.8097-7.913913.1728
264.86922.6562-1.57384.3163-0.34341.6037-0.43270.7135-0.204-0.82490.4361-0.14990.2615-0.123-0.02140.3816-0.05520.04370.3648-0.02050.2179138.80113.2351-18.9831
275.76432.7939-1.40857.0159-1.28420.6682-0.51210.75340.1787-0.86880.63830.8620.086-0.685-0.04320.3425-0.0649-0.05720.53280.10490.4185121.103412.1166-14.113
284.45551.0256-0.43262.8382-0.01821.4571-0.31190.51890.0743-0.59340.3051-0.05880.1157-0.0811-0.00920.3444-0.0920.05840.3316-0.03130.1929137.401314.4159-18.1345
292.02071.714-0.11114.2563-0.00011.3207-0.0246-0.0402-0.11510.0381-0.0205-0.39850.03350.19430.05930.21120.00580.04970.2537-0.00620.2285149.625719.8879-6.5326
304.61071.37121.64916.35250.97692.1799-0.32320.25361.0693-1.02830.2705-0.1031-0.60760.06490.02610.457-0.0040.09810.2680.05720.5585148.220946.3069-12.5314
315.62641.34610.42856.5833-1.6887.9185-0.3780.98740.3875-1.0840.201-0.85910.00270.980.13790.5247-0.07080.17130.3743-0.00950.7024152.438642.8935-16.4313
325.5221-1.4779-0.91836.60270.52712.8274-0.050.0552-0.05-0.15140.0911-0.62850.07670.28340.00680.2314-0.03820.06180.2358-0.00590.2935155.646725.517-9.9901
335.94490.3864-1.95691.06541.31132.6930.0286-0.67240.01920.35820.0918-0.2085-0.10530.1917-0.11740.336-0.0156-0.09340.2514-0.00960.2587130.240776.644220.773
343.8951-0.530.94532.09191.18131.38720.0713-0.678-0.37170.57790.00250.1190.3437-0.3552-0.04470.3636-0.02470.01570.33280.11440.2305111.743567.748619.523
353.72480.5835-0.33142.9211-0.18331.90970.0517-0.25550.00790.2745-0.0263-0.4119-0.07250.0813-0.01060.21050.0319-0.0460.1818-0.00930.2021128.149776.218514.9921
365.48662.7114-0.07736.90152.02644.3385-0.10.2245-0.1837-0.16590.1747-0.8562-0.18150.1606-0.08490.16790.0924-0.03680.14020.05070.2968138.793673.16491.3326
375.9485-3.2208-3.2563.24771.43725.2054-0.1013-0.2192-0.20420.06370.1686-0.84520.37390.4234-0.0840.25830.0084-0.05090.2123-0.01210.6739147.329956.02014.1087
384.6121-3.43184.24792.6536-2.46928.1984-0.1325-0.43830.27160.67240.0865-0.7326-0.25821.01670.0830.57810.0674-0.31980.6375-0.01111.2808159.968154.961116.6848
397.6604-1.45363.68726.613-1.14482.0268-0.01270.47560.167-0.16510.0685-1.2768-0.3441.01860.02050.2893-0.01420.09690.36850.00270.8002149.824566.01891.5864
402.4388-1.8799-1.19185.3317-1.57364.1736-0.0459-0.3838-0.39490.66540.0842-0.70930.37390.5081-0.05510.31030.0938-0.12210.33390.03850.5491146.238759.413813.7874
415.27243.51442.9335.12673.5378.519-0.0037-0.00560.05290.09120.1138-0.6666-0.26310.4832-0.07930.21540.0188-0.03040.17270.03890.4073142.891679.03717.5733
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'A' and (resid 11 through 106 )
2X-RAY DIFFRACTION2chain 'A' and (resid 107 through 198 )
3X-RAY DIFFRACTION3chain 'A' and (resid 199 through 275 )
4X-RAY DIFFRACTION4chain 'A' and (resid 276 through 330 )
5X-RAY DIFFRACTION5chain 'A' and (resid 331 through 416 )
6X-RAY DIFFRACTION6chain 'B' and (resid 11 through 46 )
7X-RAY DIFFRACTION7chain 'B' and (resid 47 through 70 )
8X-RAY DIFFRACTION8chain 'B' and (resid 71 through 106 )
9X-RAY DIFFRACTION9chain 'B' and (resid 107 through 198 )
10X-RAY DIFFRACTION10chain 'B' and (resid 199 through 247 )
11X-RAY DIFFRACTION11chain 'B' and (resid 248 through 302 )
12X-RAY DIFFRACTION12chain 'B' and (resid 303 through 330 )
13X-RAY DIFFRACTION13chain 'B' and (resid 331 through 349 )
14X-RAY DIFFRACTION14chain 'B' and (resid 350 through 416 )
15X-RAY DIFFRACTION15chain 'C' and (resid 11 through 60 )
16X-RAY DIFFRACTION16chain 'C' and (resid 61 through 106 )
17X-RAY DIFFRACTION17chain 'C' and (resid 107 through 198 )
18X-RAY DIFFRACTION18chain 'C' and (resid 199 through 247 )
19X-RAY DIFFRACTION19chain 'C' and (resid 248 through 302 )
20X-RAY DIFFRACTION20chain 'C' and (resid 303 through 330 )
21X-RAY DIFFRACTION21chain 'C' and (resid 331 through 349 )
22X-RAY DIFFRACTION22chain 'C' and (resid 350 through 417 )
23X-RAY DIFFRACTION23chain 'D' and (resid 10 through 106 )
24X-RAY DIFFRACTION24chain 'D' and (resid 107 through 317 )
25X-RAY DIFFRACTION25chain 'D' and (resid 318 through 417 )
26X-RAY DIFFRACTION26chain 'E' and (resid 11 through 70 )
27X-RAY DIFFRACTION27chain 'E' and (resid 71 through 106 )
28X-RAY DIFFRACTION28chain 'E' and (resid 107 through 162 )
29X-RAY DIFFRACTION29chain 'E' and (resid 163 through 241 )
30X-RAY DIFFRACTION30chain 'E' and (resid 242 through 312 )
31X-RAY DIFFRACTION31chain 'E' and (resid 313 through 372 )
32X-RAY DIFFRACTION32chain 'E' and (resid 373 through 416 )
33X-RAY DIFFRACTION33chain 'F' and (resid 10 through 46 )
34X-RAY DIFFRACTION34chain 'F' and (resid 47 through 106 )
35X-RAY DIFFRACTION35chain 'F' and (resid 107 through 215 )
36X-RAY DIFFRACTION36chain 'F' and (resid 216 through 241 )
37X-RAY DIFFRACTION37chain 'F' and (resid 242 through 302 )
38X-RAY DIFFRACTION38chain 'F' and (resid 303 through 330 )
39X-RAY DIFFRACTION39chain 'F' and (resid 331 through 349 )
40X-RAY DIFFRACTION40chain 'F' and (resid 350 through 383 )
41X-RAY DIFFRACTION41chain 'F' and (resid 384 through 416 )

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