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Open data
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Basic information
Entry | Database: EMDB / ID: EMD-11903 | |||||||||
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Title | Staphylococcus aureus 70S after 50 minutes incubation at 37C | |||||||||
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![]() | Ribosome / H68 / translation / protein synthesis | |||||||||
Function / homology | ![]() large ribosomal subunit / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit / small ribosomal subunit rRNA binding / transferase activity / 5S rRNA binding / large ribosomal subunit rRNA binding / cytosolic small ribosomal subunit / cytosolic large ribosomal subunit ...large ribosomal subunit / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit / small ribosomal subunit rRNA binding / transferase activity / 5S rRNA binding / large ribosomal subunit rRNA binding / cytosolic small ribosomal subunit / cytosolic large ribosomal subunit / tRNA binding / cytoplasmic translation / rRNA binding / negative regulation of translation / ribosome / structural constituent of ribosome / ribonucleoprotein complex / translation / mRNA binding / RNA binding / zinc ion binding / cytoplasm / cytosol Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 2.86 Å | |||||||||
![]() | Cimicata G / Bashan A / Yonath A | |||||||||
Funding support | 1 items
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![]() | ![]() Title: Staphylococcus aureus 50S after 30 minutes incubation a 37C Authors: Cimicata G / Bashan A / Yonath A | |||||||||
History |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 336.9 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 61.8 KB 61.8 KB | Display Display | ![]() |
Images | ![]() | 117.4 KB | ||
Filedesc metadata | ![]() | 11.8 KB | ||
Others | ![]() | 395.4 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 534.2 KB | Display | ![]() |
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Full document | ![]() | 533.8 KB | Display | |
Data in XML | ![]() | 7.6 KB | Display | |
Data in CIF | ![]() | 8.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7aspMC ![]() 7asmC ![]() 7asnC ![]() 7asoC M: atomic model generated by this map C: citing same article ( |
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Similar structure data |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
File | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Voxel size | X=Y=Z: 0.85 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
-Additional map: #1
File | emd_11903_additional_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
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Sample components
+Entire : 70S
+Supramolecule #1: 70S
+Macromolecule #1: 23S rRNA
+Macromolecule #2: 16S rRNA
+Macromolecule #3: 5S rRNA
+Macromolecule #4: 50S ribosomal protein L33,50S ribosomal protein L30
+Macromolecule #5: 50S ribosomal protein L34
+Macromolecule #6: 50S ribosomal protein L35
+Macromolecule #7: 50S ribosomal protein L36
+Macromolecule #8: 30S ribosomal protein S10
+Macromolecule #9: 30S ribosomal protein S11
+Macromolecule #10: 30S ribosomal protein S12
+Macromolecule #11: 50S ribosomal protein L2
+Macromolecule #12: 30S ribosomal protein S13
+Macromolecule #13: 50S ribosomal protein L3
+Macromolecule #14: 50S ribosomal protein L4
+Macromolecule #15: 30S ribosomal protein S14 type Z
+Macromolecule #16: 30S ribosomal protein S15
+Macromolecule #17: 30S ribosomal protein S16
+Macromolecule #18: 50S ribosomal protein L6
+Macromolecule #19: 50S ribosomal protein L13
+Macromolecule #20: 30S ribosomal protein S17
+Macromolecule #21: 50S ribosomal protein L14
+Macromolecule #22: 30S ribosomal protein S18
+Macromolecule #23: 30S ribosomal protein S20
+Macromolecule #24: 50S ribosomal protein L15
+Macromolecule #25: 30S ribosomal protein S3
+Macromolecule #26: 50S ribosomal protein L16
+Macromolecule #27: 50S ribosomal protein L17
+Macromolecule #28: 30S ribosomal protein S4
+Macromolecule #29: 30S ribosomal protein S5
+Macromolecule #30: 50S ribosomal protein L18
+Macromolecule #31: 30S ribosomal protein S6
+Macromolecule #32: 50S ribosomal protein L19
+Macromolecule #33: 30S ribosomal protein S8
+Macromolecule #34: 50S ribosomal protein L20
+Macromolecule #35: 30S ribosomal protein S7
+Macromolecule #36: 50S ribosomal protein L21
+Macromolecule #37: 30S ribosomal protein S9
+Macromolecule #38: 50S ribosomal protein L22
+Macromolecule #39: 50S ribosomal protein L23
+Macromolecule #40: 50S ribosomal protein L24
+Macromolecule #41: 50S ribosomal protein L25
+Macromolecule #42: 50S ribosomal protein L27
+Macromolecule #43: 50S ribosomal protein L28
+Macromolecule #44: 50S ribosomal protein L29
+Macromolecule #45: 50S ribosomalprotein L32p
+Macromolecule #46: 50S ribosomal protein L5
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 7.6 |
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Grid | Model: Quantifoil R1.2/1.3 / Material: COPPER / Mesh: 200 |
Vitrification | Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 47.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |