3J4J
 
 | | Model of full-length T. thermophilus Translation Initiation Factor 2 refined against its cryo-EM density from a 30S Initiation Complex map | | Descriptor: | Translation initiation factor IF-2 | | Authors: | Simonetti, A, Marzi, S, Billas, I.M.L, Tsai, A, Fabbretti, A, Myasnikov, A, Roblin, P, Vaiana, A.C, Hazemann, I, Eiler, D, Steitz, T.A, Puglisi, J.D, Gualerzi, C.O, Klaholz, B.P. | | Deposit date: | 2013-08-26 | | Release date: | 2013-09-25 | | Last modified: | 2024-02-21 | | Method: | ELECTRON MICROSCOPY (11.5 Å) | | Cite: | Involvement of protein IF2 N domain in ribosomal subunit joining revealed from architecture and function of the full-length initiation factor.
Proc.Natl.Acad.Sci.USA, 110, 2013
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4V9R
 | | Crystal structure of antibiotic DITYROMYCIN bound to 70S ribosome | | Descriptor: | 16S Ribosomal RNA, 23S Ribosomal RNA, 30S Ribosomal Protein S10, ... | | Authors: | Bulkley, D.P, Brandi, L, Polikanov, Y.S, Fabbretti, A, O'Connor, M, Gualerzi, C.O, Steitz, T.A. | | Deposit date: | 2013-12-05 | | Release date: | 2014-07-09 | | Last modified: | 2025-03-26 | | Method: | X-RAY DIFFRACTION (3 Å) | | Cite: | The antibiotics dityromycin and GE82832 bind protein S12 and block EF-G-catalyzed translocation.
Cell Rep, 6, 2014
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4V9S
 | | Crystal structure of antibiotic GE82832 bound to 70S ribosome | | Descriptor: | 16S Ribosomal RNA, 23S Ribosomal RNA, 30S Ribosomal Protein S10, ... | | Authors: | Bulkley, D.P, Brandi, L, Polikanov, Y.S, Fabbretti, A, O'Connor, M, Gualerzi, C.O, Steitz, T.A. | | Deposit date: | 2013-12-05 | | Release date: | 2014-07-09 | | Last modified: | 2025-03-26 | | Method: | X-RAY DIFFRACTION (3.1 Å) | | Cite: | The antibiotics dityromycin and GE82832 bind protein S12 and block EF-G-catalyzed translocation.
Cell Rep, 6, 2014
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5ME0
 | | Structure of the 30S Pre-Initiation Complex 1 (30S IC-1) Stalled by GE81112 | | Descriptor: | 16S ribosomal RNA, 30S ribosomal protein S10, 30S ribosomal protein S11, ... | | Authors: | Lopez-Alonso, J.P, Fabbretti, A, Kaminishi, T, Iturrioz, I, Brandi, L, Gil Carton, D, Gualerzi, C, Fucini, P, Connell, S. | | Deposit date: | 2016-11-14 | | Release date: | 2017-01-11 | | Last modified: | 2024-04-24 | | Method: | ELECTRON MICROSCOPY (13.5 Å) | | Cite: | Structure of a 30S pre-initiation complex stalled by GE81112 reveals structural parallels in bacterial and eukaryotic protein synthesis initiation pathways.
Nucleic Acids Res., 45, 2017
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5ME1
 | | Structure of the 30S Pre-Initiation Complex 2 (30S IC-2) Stalled by GE81112 | | Descriptor: | 16S ribosomal RNA, 30S ribosomal protein S10, 30S ribosomal protein S11, ... | | Authors: | Lopez-Alonso, J.P, Fabbretti, A, Kaminishi, T, Iturrioz, I, Brandi, L, Gil Carton, D, Gualerzi, C, Fucini, P, Connell, S. | | Deposit date: | 2016-11-14 | | Release date: | 2017-01-11 | | Last modified: | 2024-04-24 | | Method: | ELECTRON MICROSCOPY (13.5 Å) | | Cite: | Structure of a 30S pre-initiation complex stalled by GE81112 reveals structural parallels in bacterial and eukaryotic protein synthesis initiation pathways.
Nucleic Acids Res., 45, 2017
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5IWA
 | | Crystal structure of the 30S ribosomal subunit from Thermus thermophilus in complex with the GE81112 peptide antibiotic | | Descriptor: | (2S,3S)-2-{[(2S)-3-(2-amino-1H-imidazol-5-yl)-2-{[(2S,4S)-5-(carbamoyloxy)-4-hydroxy-2-({[(2S,3S)-3-hydroxypiperidin-2-yl]carbonyl}amino)pentanoyl]amino}propanoyl]amino}-3-(2-chloro-1H-imidazol-5-yl)-3-hydroxypropanoic acid, 16S ribosomal RNA, 30S ribosomal protein S10, ... | | Authors: | Schedlbauer, A, Kaminishi, T, Ochoa-Lizarralde, B, Chieko, N, Masahito, K, Takemoto, C, Yokoyama, S, Connell, S.R, Fucini, P. | | Deposit date: | 2016-03-22 | | Release date: | 2016-04-27 | | Last modified: | 2024-01-10 | | Method: | X-RAY DIFFRACTION (3.5 Å) | | Cite: | Inhibition of translation initiation complex formation by GE81112 unravels a 16S rRNA structural switch involved in P-site decoding.
Proc.Natl.Acad.Sci.USA, 113, 2016
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5DM6
 | | Crystal structure of the 50S ribosomal subunit from Deinococcus radiodurans | | Descriptor: | 23S ribosomal RNA, 50S ribosomal protein L1, 50S ribosomal protein L11, ... | | Authors: | Kaminishi, T, Schedlbauer, A, Ochoa-Lizarralde, B, Connell, S.R, Fucini, P. | | Deposit date: | 2015-09-08 | | Release date: | 2015-11-11 | | Last modified: | 2024-11-13 | | Method: | X-RAY DIFFRACTION (2.9 Å) | | Cite: | Crystallographic characterization of the ribosomal binding site and molecular mechanism of action of Hygromycin A.
Nucleic Acids Res., 43, 2015
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5DM7
 | | Crystal structure of the 50S ribosomal subunit from Deinococcus radiodurans in complex with hygromycin A | | Descriptor: | 23S ribosomal RNA, 50S ribosomal protein L1, 50S ribosomal protein L11, ... | | Authors: | Kaminishi, T, Schedlbauer, A, Ochoa-Lizarralde, B, Connell, S.R, Fucini, P. | | Deposit date: | 2015-09-08 | | Release date: | 2015-11-11 | | Last modified: | 2024-11-20 | | Method: | X-RAY DIFFRACTION (3 Å) | | Cite: | Crystallographic characterization of the ribosomal binding site and molecular mechanism of action of Hygromycin A.
Nucleic Acids Res., 43, 2015
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9FDA
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9FCO
 | | Structure of E. coli 30S-IF1-IF3-mRNA-Kasugamycin complex | | Descriptor: | (1S,2R,3S,4R,5S,6S)-2,3,4,5,6-PENTAHYDROXYCYCLOHEXYL 2-AMINO-4-{[CARBOXY(IMINO)METHYL]AMINO}-2,3,4,6-TETRADEOXY-ALPHA-D-ARABINO-HEXOPYRANOSIDE, 16S rRNA, MAGNESIUM ION, ... | | Authors: | Safdari, H.A, Wilson, D.N. | | Deposit date: | 2024-05-15 | | Release date: | 2025-03-19 | | Method: | ELECTRON MICROSCOPY (2.4 Å) | | Cite: | The translation inhibitors kasugamycin, edeine and GE81112 target distinct steps during 30S initiation complex formation.
Nat Commun, 16, 2025
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9G06
 | | Structure of 30S-IF1-IF3-mRNA-fMet-tRNA-GE81112A complex | | Descriptor: | (2S,3S)-2-[[(2S)-2-[[(2S,4S)-5-aminocarbonyloxy-4-oxidanyl-2-[[(2S,3R)-3-oxidanylpiperidin-2-yl]carbonylamino]pentanoyl]amino]-3-(1H-imidazol-4-yl)propanoyl]amino]-3-(2-chloranyl-1H-imidazol-4-yl)-3-oxidanyl-propanoic acid, 16S ribosomal RNA, MAGNESIUM ION, ... | | Authors: | Safdari, H.A, Morici, M, Wilson, D.N. | | Deposit date: | 2024-07-07 | | Release date: | 2025-03-19 | | Method: | ELECTRON MICROSCOPY (2.85 Å) | | Cite: | The translation inhibitors kasugamycin, edeine and GE81112 target distinct steps during 30S initiation complex formation.
Nat Commun, 16, 2025
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9FIB
 | | Structure of 30S-IF1-IF3-mRNA-GE81112A complex | | Descriptor: | (2S,3S)-2-[[(2S)-2-[[(2S,4S)-5-aminocarbonyloxy-4-oxidanyl-2-[[(2S,3R)-3-oxidanylpiperidin-2-yl]carbonylamino]pentanoyl]amino]-3-(1H-imidazol-4-yl)propanoyl]amino]-3-(2-chloranyl-1H-imidazol-4-yl)-3-oxidanyl-propanoic acid, 16S rRNA, MAGNESIUM ION, ... | | Authors: | Safdari, H.A, Morici, M, Wilson, D.N. | | Deposit date: | 2024-05-29 | | Release date: | 2025-03-19 | | Method: | ELECTRON MICROSCOPY (2.3 Å) | | Cite: | The translation inhibitors kasugamycin, edeine and GE81112 target distinct steps during 30S initiation complex formation.
Nat Commun, 16, 2025
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4B3X
 | | Bacterial translation initiation factor IF2 (1-363), apo form | | Descriptor: | GLYCEROL, TRANSLATION INITIATION FACTOR IF-2 | | Authors: | Simonetti, A, Urzhumtsev, A, Klaholz, B.P. | | Deposit date: | 2012-07-26 | | Release date: | 2013-05-29 | | Last modified: | 2024-05-08 | | Method: | X-RAY DIFFRACTION (1.95 Å) | | Cite: | Structure of the Protein Core of Translation Initiation Factor 2 in Apo, GTP-Bound and Gdp-Bound Forms
Acta Crystallogr.,Sect.D, 69, 2013
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4B48
 | | Bacterial translation initiation factor IF2 (1-363), complex with GTP | | Descriptor: | GUANOSINE-5'-TRIPHOSPHATE, MAGNESIUM ION, TRANSLATION INITIATION FACTOR IF-2 | | Authors: | Simonetti, A, Urzhumtsev, A, Klaholz, B.P. | | Deposit date: | 2012-07-27 | | Release date: | 2013-05-29 | | Last modified: | 2023-12-20 | | Method: | X-RAY DIFFRACTION (2.8 Å) | | Cite: | Structure of the Protein Core of Translation Initiation Factor 2 in Apo, GTP-Bound and Gdp-Bound Forms
Acta Crystallogr.,Sect.D, 69, 2013
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4B43
 | | Bacterial translation initiation factor IF2 (1-363), apo form, double mutant K86L H130A | | Descriptor: | GLYCEROL, IODIDE ION, MAGNESIUM ION, ... | | Authors: | Simonetti, A, Urzhumtsev, A, Klaholz, B.P. | | Deposit date: | 2012-07-27 | | Release date: | 2013-09-18 | | Last modified: | 2023-12-20 | | Method: | X-RAY DIFFRACTION (1.937 Å) | | Cite: | Molecular Basis of GTP Hydrolysis by Bacterial Translation Initiation Factor If2
To be Published
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4B44
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4B47
 | | Bacterial translation initiation factor IF2 (1-363), complex with GDP at pH6.5 | | Descriptor: | GUANOSINE-5'-DIPHOSPHATE, MAGNESIUM ION, TRANSLATION INITIATION FACTOR IF-2 | | Authors: | Simonetti, A, Urzhumtsev, A, Klaholz, B.P. | | Deposit date: | 2012-07-27 | | Release date: | 2013-05-29 | | Last modified: | 2023-12-20 | | Method: | X-RAY DIFFRACTION (2.301 Å) | | Cite: | Structure of the Protein Core of Translation Initiation Factor 2 in Apo, GTP-Bound and Gdp-Bound Forms
Acta Crystallogr.,Sect.D, 69, 2013
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