5ME1

Structure of the 30S Pre-Initiation Complex 2 (30S IC-2) Stalled by GE81112

Summary for 5ME1

EMDB information3495
Descriptor16S ribosomal RNA, 30S ribosomal protein S2, 30S ribosomal protein S3, ... (27 entities in total)
Functional Keywordsribosome, initiation of translation
Biological sourceEscherichia coli K-12
More
Total number of polymer chains26
Total molecular weight953587.7
Authors
Lopez-Alonso, J.P.,Fabbretti, A.,Kaminishi, T.,Iturrioz, I.,Brandi, L.,Gil Carton, D.,Gualerzi, C.,Fucini, P.,Connell, S. (deposition date: 2016-11-14, release date: 2017-01-11, Last modification date: 2018-11-21)
Primary citation
Lopez-Alonso, J.P.,Fabbretti, A.,Kaminishi, T.,Iturrioz, I.,Brandi, L.,Gil-Carton, D.,Gualerzi, C.O.,Fucini, P.,Connell, S.R.
Structure of a 30S pre-initiation complex stalled by GE81112 reveals structural parallels in bacterial and eukaryotic protein synthesis initiation pathways.
Nucleic Acids Res., 45:2179-2187, 2017
PubMed: 27986852 (PDB entries with the same primary citation)
DOI: 10.1093/nar/gkw1251
MImport into Mendeley
Experimental method
ELECTRON MICROSCOPY
?

Structure validation

ClashscoreRamachandran outliersSidechain outliersRNA backbone19 0.9% 3.2% 0.55MetricValuePercentile RanksWorseBetterPercentile relative to all structuresPercentile relative to all EM structures

More Asymmetric unit images

Molmil generated image of 5me1
no rotation
Molmil generated image of 5me1
rotated about x axis by 90°
Molmil generated image of 5me1
rotated about y axis by 90°