5ME1
Structure of the 30S Pre-Initiation Complex 2 (30S IC-2) Stalled by GE81112
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK II |
EM Data Collection
| Microscope | JEOL 2200FSC |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2 |
| Energy Filter | In-column Omega Filter |
| Detector | GATAN ULTRASCAN 4000 (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 13.5 |
| Number of Particle | 174142 |
| Software | RELION,Coot,DigitalMicrograph,CTFFIND,Situs |
| Symmetry | C1 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
