3UV5
Crystal Structure of the tandem bromodomains of human Transcription initiation factor TFIID subunit 1 (TAF1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-11-27 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 57.760, 199.990, 88.020 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.480 - 2.030 |
R-factor | 0.192 |
Rwork | 0.190 |
R-free | 0.22970 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Ensemble of PDB entries 3HMH 2grc 2oo1 2oss 2ouo 3dai 3d7c 3dwy 3g0l 3gg3 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.649 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.16) |
Phasing software | PHASER (2.1.4) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 34.482 | 34.482 | 2.140 |
High resolution limit [Å] | 2.030 | 6.420 | 2.030 |
Rmerge | 0.092 | 0.039 | 0.741 |
Total number of observations | 7243 | 20411 | |
Number of reflections | 33176 | ||
<I/σ(I)> | 11.5 | 15.1 | 1 |
Completeness [%] | 99.2 | 97.4 | 95.1 |
Redundancy | 6.5 | 6.4 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9 | 293 | 0.1M Tris-HCl, 1.1M (NH4)2SO4, pH 9.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |