3V1Z
 
 | | Crystal structure of Type IIF restriction endonuclease Bse634I with cognate DNA | | Descriptor: | DNA (5'-D(*TP*CP*GP*CP*GP*CP*CP*GP*GP*CP*GP*CP*G)-3'), Endonuclease Bse634IR | | Authors: | Manakova, E.N, Grazulis, S, Golovenko, D, Tamulaitiene, G. | | Deposit date: | 2011-12-11 | | Release date: | 2012-04-25 | | Last modified: | 2023-09-13 | | Method: | X-RAY DIFFRACTION (2.2 Å) | | Cite: | Structural mechanisms of the degenerate sequence recognition by Bse634I restriction endonuclease.
Nucleic Acids Res., 40, 2012
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4C3G
 | | cryo-EM structure of activated and oligomeric restriction endonuclease SgrAI | | Descriptor: | 5'-D(*CP*CP*GP*GP*TP*GP*TP*GP*AP*AP*GP*AP*CP*CP *CP*AP*CP*GP*CP*AP*TP*CP)-3', 5'-D(*GP*AP*TP*GP*CP*GP*TP*GP*GP*GP*TP*CP*TP*TP *CP*AP*CP*AP)-3', SGRAIR RESTRICTION ENZYME | | Authors: | Lyumkis, D, Talley, H, Stewart, A, Shah, S, Park, C.K, Tama, F, Potter, C.S, Carragher, B, Horton, N.C. | | Deposit date: | 2013-08-23 | | Release date: | 2013-09-11 | | Last modified: | 2024-05-08 | | Method: | ELECTRON MICROSCOPY (8.6 Å) | | Cite: | Allosteric Regulation of DNA Cleavage and Sequence-Specificity Through Run-on Oligomerization.
Structure, 21, 2013
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6OBJ
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1KNV
 | | Bse634I restriction endonuclease | | Descriptor: | ACETATE ION, Bse634I restriction endonuclease, CHLORIDE ION | | Authors: | Grazulis, S, Deibert, M, Rimseliene, R, Skirgaila, R, Sasnauskas, G, Lagunavicius, A, Repin, V, Urbanke, C, Huber, R, Siksnys, V. | | Deposit date: | 2001-12-19 | | Release date: | 2002-02-27 | | Last modified: | 2024-03-13 | | Method: | X-RAY DIFFRACTION (2.17 Å) | | Cite: | Crystal structure of the Bse634I restriction endonuclease: comparison of two enzymes recognizing the same DNA sequence.
Nucleic Acids Res., 30, 2002
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1CFR
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3V20
 | | Crystal structure of Type IIF restriction endonuclease Bse634I with cognate DNA | | Descriptor: | 2-(2-(2-(2-(2-(2-ETHOXYETHOXY)ETHOXY)ETHOXY)ETHOXY)ETHOXY)ETHANOL, CALCIUM ION, CHLORIDE ION, ... | | Authors: | Manakova, E.N, Grazulis, S, Golovenko, D, Tamulaitiene, G. | | Deposit date: | 2011-12-11 | | Release date: | 2012-04-25 | | Last modified: | 2023-09-13 | | Method: | X-RAY DIFFRACTION (2.35 Å) | | Cite: | Structural mechanisms of the degenerate sequence recognition by Bse634I restriction endonuclease.
Nucleic Acids Res., 40, 2012
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7S8D
 | | Structure of DNA-free SgrAI | | Descriptor: | CALCIUM ION, SgraIR restriction enzyme | | Authors: | Horton, N.C. | | Deposit date: | 2021-09-17 | | Release date: | 2022-08-24 | | Last modified: | 2023-10-18 | | Method: | X-RAY DIFFRACTION (2.02 Å) | | Cite: | Pretransition state and apo structures of the filament-forming enzyme SgrAI elucidate mechanisms of activation and substrate specificity.
J.Biol.Chem., 298, 2022
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9BGI
 | | Activated wild-type SgrAI endonuclease DNA-bound dimer with Mg2+ and cleaved primary site DNA | | Descriptor: | 40-1 DNA, MAGNESIUM ION, SgraIR restriction enzyme | | Authors: | Shan, Z, Lyumkis, D, Horton, N.C. | | Deposit date: | 2024-04-18 | | Release date: | 2025-03-05 | | Method: | ELECTRON MICROSCOPY (3.05 Å) | | Cite: | Two-metal ion mechanism of DNA cleavage by activated, filamentous SgrAI.
J.Biol.Chem., 300, 2024
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9BGJ
 | | SgrAI mutant K242A endonuclease DNA-bound dimer with Mg2+ and intact primary site DNA | | Descriptor: | 40-1 DNA, MAGNESIUM ION, SgraIR restriction enzyme | | Authors: | Shan, Z, Lyumkis, D, Horton, N.C. | | Deposit date: | 2024-04-18 | | Release date: | 2025-03-05 | | Method: | ELECTRON MICROSCOPY (3.34 Å) | | Cite: | Two-metal ion mechanism of DNA cleavage by activated, filamentous SgrAI.
J.Biol.Chem., 300, 2024
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7SS5
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3V21
 | | Crystal structure of Type IIF restriction endonuclease Bse634I with cognate DNA | | Descriptor: | DNA (5'-D(*TP*TP*CP*GP*AP*CP*CP*GP*GP*TP*CP*GP*A)-3'), Endonuclease Bse634IR | | Authors: | Manakova, E.N, Grazulis, S, Golovenko, D, Tamulaitiene, G. | | Deposit date: | 2011-12-11 | | Release date: | 2012-04-25 | | Last modified: | 2023-09-13 | | Method: | X-RAY DIFFRACTION (2.7 Å) | | Cite: | Structural mechanisms of the degenerate sequence recognition by Bse634I restriction endonuclease.
Nucleic Acids Res., 40, 2012
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3DVO
 | | SgrAI with cognate DNA and calcium bound | | Descriptor: | CALCIUM ION, DNA (5'-D(*DGP*DAP*DGP*DTP*DCP*DCP*DAP*DCP*DCP*DGP*DGP*DTP*DGP*DGP*DAP*DCP*DTP*DC)-3'), SgraIR restriction enzyme | | Authors: | Dunten, P.W, Horton, N.C, Little, E.J. | | Deposit date: | 2008-07-18 | | Release date: | 2008-08-19 | | Last modified: | 2023-08-30 | | Method: | X-RAY DIFFRACTION (1.892 Å) | | Cite: | The structure of SgrAI bound to DNA; recognition of an 8 base pair target.
Nucleic Acids Res., 36, 2008
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3DW9
 | | SgrAI with cognate DNA and manganese bound | | Descriptor: | DNA (5'-D(*DGP*DAP*DGP*DTP*DCP*DCP*DAP*DCP*DCP*DGP*DGP*DTP*DGP*DGP*DAP*DCP*DTP*DC)-3'), MANGANESE (II) ION, SgraIR restriction enzyme | | Authors: | Dunten, P.W, Horton, N.C, Little, E.J. | | Deposit date: | 2008-07-21 | | Release date: | 2008-08-19 | | Last modified: | 2024-02-21 | | Method: | X-RAY DIFFRACTION (2.2 Å) | | Cite: | The structure of SgrAI bound to DNA; recognition of an 8 base pair target.
Nucleic Acids Res., 36, 2008
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3DPG
 | | SgrAI with noncognate DNA bound | | Descriptor: | CALCIUM ION, DNA (5'-D(*DAP*DAP*DGP*DTP*DCP*DGP*DAP*DCP*DCP*DGP*DGP*DTP*DGP*DGP*DAP*DCP*DT)-3'), SgraIR restriction enzyme | | Authors: | Dunten, P.W, Horton, N.C, Little, E.J. | | Deposit date: | 2008-07-08 | | Release date: | 2008-08-19 | | Last modified: | 2024-02-21 | | Method: | X-RAY DIFFRACTION (1.91 Å) | | Cite: | The structure of SgrAI bound to DNA; recognition of an 8 base pair target.
Nucleic Acids Res., 36, 2008
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3MQY
 | | SgrAI with cleaved DNA and Magnesium bound | | Descriptor: | DNA (5'-D(*GP*AP*GP*TP*CP*CP*A)-3'), DNA (5'-D(P*CP*CP*GP*GP*TP*GP*GP*AP*CP*TP*C)-3'), MAGNESIUM ION, ... | | Authors: | Dunten, P.W, Horton, N.C, Little, E.J. | | Deposit date: | 2010-04-28 | | Release date: | 2010-11-24 | | Last modified: | 2023-09-06 | | Method: | X-RAY DIFFRACTION (2 Å) | | Cite: | New clues in the allosteric activation of DNA cleavage by SgrAI: structures of SgrAI bound to cleaved primary-site DNA and uncleaved secondary-site DNA.
Acta Crystallogr.,Sect.D, 67, 2011
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3N7B
 | | SgrAI bound to secondary site DNA and Ca(II) | | Descriptor: | CALCIUM ION, DNA (5'-D(*AP*GP*TP*CP*CP*AP*CP*CP*GP*GP*GP*GP*GP*AP*CP*T)-3'), DNA (5'-D(*AP*GP*TP*CP*CP*CP*CP*CP*GP*GP*TP*GP*GP*AP*CP*T)-3'), ... | | Authors: | Horton, N.C, Little, E.J, Dunten, P.W. | | Deposit date: | 2010-05-26 | | Release date: | 2010-11-24 | | Last modified: | 2024-02-21 | | Method: | X-RAY DIFFRACTION (2.65 Å) | | Cite: | New clues in the allosteric activation of DNA cleavage by SgrAI: structures of SgrAI bound to cleaved primary-site DNA and uncleaved secondary-site DNA.
Acta Crystallogr.,Sect.D, 67, 2011
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3MQ6
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3N78
 | | SgrAI bound to Secondary Site DNA and Mg(II) | | Descriptor: | DNA (5'-D(*AP*GP*TP*CP*CP*AP*CP*CP*GP*GP*GP*GP*GP*AP*CP*T)-3'), DNA (5'-D(*AP*GP*TP*CP*CP*CP*CP*CP*GP*GP*TP*GP*GP*AP*CP*T)-3'), MAGNESIUM ION, ... | | Authors: | Horton, N.C, Little, E.J, Dunten, P.W. | | Deposit date: | 2010-05-26 | | Release date: | 2010-11-24 | | Last modified: | 2024-02-21 | | Method: | X-RAY DIFFRACTION (2.95 Å) | | Cite: | New clues in the allosteric activation of DNA cleavage by SgrAI: structures of SgrAI bound to cleaved primary-site DNA and uncleaved secondary-site DNA.
Acta Crystallogr.,Sect.D, 67, 2011
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