3N7B

SgrAI bound to secondary site DNA and Ca(II)

Summary for 3N7B

• Entry DOI: 10.2210/pdb3n7b/pdb
• Related: 3DPG 3DVO 3DW9 3MQY 3N78
• Descriptor: SgraIR restriction enzyme, DNA (5'-D(*AP*GP*TP*CP*CP*AP*CP*CP*GP*GP*GP*GP*GP*AP*CP*T)-3'), DNA (5'-D(*AP*GP*TP*CP*CP*CP*CP*CP*GP*GP*TP*GP*GP*AP*CP*T)-3'), ... (5 entities in total)
• Functional Keywords: restriction endonuclease, hydrolase-dna complex, hydrolase/dna
• Biological source: Streptomyces griseus
• Total number of polymer chains: 4
• Total formula weight: 85803.39

Authors

Horton, N.C.,Little, E.J.,Dunten, P.W. (deposition date: 2010-05-26, release date: 2010-11-24, Last modification date: 2024-02-21)

• Primary citation: Little, E.J.,Dunten, P.W.,Bitinaite, J.,Horton, N.C.; New clues in the allosteric activation of DNA cleavage by SgrAI: structures of SgrAI bound to cleaved primary-site DNA and uncleaved secondary-site DNA.; Acta Crystallogr.,Sect.D, 67:67-74, 2011; PubMed: 21206063; DOI: 10.1107/S0907444910047785

Experimental method

X-RAY DIFFRACTION (2.65 Å)