3N7B

SgrAI bound to secondary site DNA and Ca(II)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	SgraIR restriction enzyme	polymer	338	37941.9	2	UniProt (Q9F6L0) Pfam (PF07832)	Streptomyces griseus
2	C (C)	DNA (5'-D(*AP*GP*TP*CP*CP*AP*CP*CP*GP*GP*GP*GP*GP*AP*CP*T)-3')	polymer	16	4924.2	1
3	D (D)	DNA (5'-D(*AP*GP*TP*CP*CP*CP*CP*CP*GP*GP*TP*GP*GP*AP*CP*T)-3')	polymer	16	4875.2	1
4	E, F, G (A, B)	CALCIUM ION	non-polymer		40.1	3	Chemie (CA)
5	H, I, J, K (A, B, C, D)	water	water		18.0	395	Chemie (HOH)

Sequence modifications

A, B: 2 - 339 (UniProt: Q9F6L0)

PDB	External Database	Details
Asp 63	Asn 63	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	85683.2
Non-Polymers*	Number of molecules	3
	Total formula weight	120.2
All*	Total formula weight	85803.4

*Water molecules are not included.