7SS5

Activated SgrAI endonuclease DNA-bound dimer with Ca2+ and intact primary site DNA

Summary for 7SS5

• Entry DOI: 10.2210/pdb7ss5/pdb
• EMDB information: 25404
• Descriptor: SgraIR restriction enzyme, DNA (5'-D(P*GP*GP*TP*CP*TP*TP*CP*AP*CP*AP*CP*CP*GP*GP*TP*GP*TP*GP*AP*AP*GP*AP*CP*C)-3'), CALCIUM ION, ... (4 entities in total)
• Functional Keywords: restriction endonuclease, dnase, allostery, bacterial innate immunity, filament, hyper-activation, substrate specificity, hydrolase-dna complex, hydrolase/dna
• Biological source: Streptomyces griseus; More
• Total number of polymer chains: 4
• Total formula weight: 104438.04

Authors

Shan, Z.,Lyumkis, D.,Horton, N.C. (deposition date: 2021-11-09, release date: 2021-12-01, Last modification date: 2024-06-05)

• Primary citation: Shan, Z.,Ghadirian, N.,Lyumkis, D.,Horton, N.C.; Pretransition state and apo structures of the filament-forming enzyme SgrAI elucidate mechanisms of activation and substrate specificity.; J.Biol.Chem., 298:101760-101760, 2022; PubMed: 35202658; DOI: 10.1016/j.jbc.2022.101760

Experimental method

ELECTRON MICROSCOPY (2.7 Å)