7SS5
Activated SgrAI endonuclease DNA-bound dimer with Ca2+ and intact primary site DNA
Summary for 7SS5
Entry DOI | 10.2210/pdb7ss5/pdb |
EMDB information | 25404 |
Descriptor | SgraIR restriction enzyme, DNA (5'-D(P*GP*GP*TP*CP*TP*TP*CP*AP*CP*AP*CP*CP*GP*GP*TP*GP*TP*GP*AP*AP*GP*AP*CP*C)-3'), CALCIUM ION, ... (4 entities in total) |
Functional Keywords | restriction endonuclease, dnase, allostery, bacterial innate immunity, filament, hyper-activation, substrate specificity, hydrolase-dna complex, hydrolase/dna |
Biological source | Streptomyces griseus More |
Total number of polymer chains | 4 |
Total formula weight | 104438.04 |
Authors | Shan, Z.,Lyumkis, D.,Horton, N.C. (deposition date: 2021-11-09, release date: 2021-12-01, Last modification date: 2024-06-05) |
Primary citation | Shan, Z.,Ghadirian, N.,Lyumkis, D.,Horton, N.C. Pretransition state and apo structures of the filament-forming enzyme SgrAI elucidate mechanisms of activation and substrate specificity. J.Biol.Chem., 298:101760-101760, 2022 Cited by PubMed: 35202658DOI: 10.1016/j.jbc.2022.101760 PDB entries with the same primary citation |
Experimental method | ELECTRON MICROSCOPY (2.7 Å) |
Structure validation
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