7SS5
Activated SgrAI endonuclease DNA-bound dimer with Ca2+ and intact primary site DNA
EM specimen
| Vitrification | Yes |
| Instrument | HOMEMADE PLUNGER |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Energy Filter | GIF Bioquantum |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 2.7 |
| Software | RELION,PHENIX,Leginon,Warp,Coot,cryoSPARC |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
