7SS5

Activated SgrAI endonuclease DNA-bound dimer with Ca2+ and intact primary site DNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, B)	SgraIR restriction enzyme	polymer	352	39783.9	2	UniProt (Q9F6L0) Pfam (PF07832)	Streptomyces griseus
2	B, D (C, D)	DNA (5'-D(P*GP*GP*TP*CP*TP*TP*CP*AP*CP*AP*CP*CP*GP*GP*TP*GP*TP*GP*AP*AP*GP*AP*CP*C)-3')	polymer	40	12314.9	2		Streptomyces griseus
3	E, F, G, H, I... (A, B)	CALCIUM ION	non-polymer		40.1	6	Chemie (CA)
4	K, L, M, N (A, C, B, D)	water	water		18.0	593	Chemie (HOH)

Sequence modifications

A, B: 1 - 339 (UniProt: Q9F6L0)

PDB	External Database	Details
Asp 63	Asn 63	conflict
Glu 340	-	expression tag
Asn 341	-	expression tag
Leu 342	-	expression tag
Tyr 343	-	expression tag
Phe 344	-	expression tag
Gln 345	-	expression tag
Ser 346	-	expression tag
His 347	-	expression tag
His 348	-	expression tag
His 349	-	expression tag
His 350	-	expression tag
His 351	-	expression tag
His 352	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	104197.6
Non-Polymers*	Number of molecules	6
	Total formula weight	240.5
All*	Total formula weight	104438.0

*Water molecules are not included.