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3Q9N
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In silico and in vitro co-evolution of a high affinity complementary protein-protein interface
分子名称: CARBAMOYL SARCOSINE, COENZYME A, CoA binding protein, ...
著者Karanicolas, J, Corn, J.E, Chen, I, Joachimiak, L.A, Dym, O, Chung, S, Albeck, S, Unger, T, Hu, W, Liu, G, Delbecq, S, Montelione, G.T, Spiegel, C, Liu, D, Baker, D, Israel Structural Proteomics Center (ISPC)
登録日2011-01-09
公開日2011-04-27
最終更新日2023-09-13
実験手法X-RAY DIFFRACTION (2 Å)
主引用文献A de novo protein binding pair by computational design and directed evolution.
Mol.Cell, 42, 2011
6FKX
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BU of 6fkx by Molmil
Crystal structure of an acetyl xylan esterase from a desert metagenome
分子名称: 2-(N-MORPHOLINO)-ETHANESULFONIC ACID, Acetyl xylan esterase, FORMIC ACID, ...
著者Adesioye, F.A, Makhalanyane, T.P, Vikram, S, Sewell, B.T, Schubert, W, Cowan, D.A.
登録日2018-01-24
公開日2018-02-28
最終更新日2024-01-17
実験手法X-RAY DIFFRACTION (2.03 Å)
主引用文献Structural Characterization and Directed Evolution of a Novel Acetyl Xylan Esterase Reveals Thermostability Determinants of the Carbohydrate Esterase 7 Family.
Appl. Environ. Microbiol., 84, 2018
8J1X
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BU of 8j1x by Molmil
A near-infrared fluorescent protein of de novo backbone design
分子名称: near-infrared fluorescent protein
著者Hu, X, Xu, Y.
登録日2023-04-13
公開日2023-10-04
最終更新日2024-05-01
実験手法X-RAY DIFFRACTION (2.25 Å)
主引用文献Using Protein Design and Directed Evolution to Monomerize a Bright Near-Infrared Fluorescent Protein.
Acs Synth Biol, 13, 2024
8J1W
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BU of 8j1w by Molmil
A near-infrared fluorescent protein of de novo backbone design
分子名称: near-infrared fluorescent protein
著者Hu, X, Xu, Y.
登録日2023-04-13
公開日2023-10-04
最終更新日2024-05-01
実験手法X-RAY DIFFRACTION (1.95 Å)
主引用文献Using Protein Design and Directed Evolution to Monomerize a Bright Near-Infrared Fluorescent Protein.
Acs Synth Biol, 13, 2024
3A4G
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BU of 3a4g by Molmil
Structure of cytochrome P450 vdh from Pseudonocardia autotrophica (trigonal crystal form)
分子名称: 2-(2-METHOXYETHOXY)ETHANOL, PROTOPORPHYRIN IX CONTAINING FE, Vitamin D hydroxylase
著者Yasutake, Y, Fujii, Y, Cheon, W.K, Arisawa, A, Tamura, T.
登録日2009-07-07
公開日2010-07-14
最終更新日2023-11-01
実験手法X-RAY DIFFRACTION (1.75 Å)
主引用文献Structural evidence for enhancement of sequential vitamin D3 hydroxylation activities by directed evolution of cytochrome P450 vitamin D3 hydroxylase
J.Biol.Chem., 285, 2010
8ELI
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BU of 8eli by Molmil
Broadly neutralizing antibody VRC34-combo.1 in complex with HIV fusion peptide (residue 512-519)
分子名称: Fusion peptide, VRC34-combo.1 Fab Heavy chain, VRC34-combo.1 Fab Light chain
著者Xu, K, Kwong, P.D.
登録日2022-09-24
公開日2023-09-27
最終更新日2024-02-14
実験手法X-RAY DIFFRACTION (1.49 Å)
主引用文献Antibody-directed evolution reveals a mechanism for enhanced neutralization at the HIV-1 fusion peptide site.
Nat Commun, 14, 2023
8EUW
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BU of 8euw by Molmil
Cryo-EM structure of HIV-1 BG505 DS-SOSIP ENV trimer bound to VRC34.01-MM28 FAB
分子名称: 2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, Envelope glycoprotein gp120, ...
著者Pletnev, S, Kwong, P.
登録日2022-10-19
公開日2023-09-27
最終更新日2024-02-14
実験手法ELECTRON MICROSCOPY (2.7 Å)
主引用文献Antibody-directed evolution reveals a mechanism for enhanced neutralization at the HIV-1 fusion peptide site.
Nat Commun, 14, 2023
8EUV
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Cryo-EM structure of HIV-1 BG505 DS-SOSIP ENV trimer bound to VRC34.01-COMBO1 FAB
分子名称: 2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, Envelope glycoprotein gp120, ...
著者Pletnev, S, Kwong, P.
登録日2022-10-19
公開日2023-09-27
最終更新日2024-02-14
実験手法ELECTRON MICROSCOPY (2.6 Å)
主引用文献Antibody-directed evolution reveals a mechanism for enhanced neutralization at the HIV-1 fusion peptide site.
Nat Commun, 14, 2023
8EUU
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BU of 8euu by Molmil
Cryo-EM structure of HIV-1 BG505 DS-SOSIP ENV trimer bound to VRC34.01 FAB
分子名称: 2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, Envelope glycoprotein gp120, ...
著者Pletnev, S, Kwong, P.
登録日2022-10-19
公開日2023-09-27
最終更新日2024-02-14
実験手法ELECTRON MICROSCOPY (2.7 Å)
主引用文献Antibody-directed evolution reveals a mechanism for enhanced neutralization at the HIV-1 fusion peptide site.
Nat Commun, 14, 2023
6I5T
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BU of 6i5t by Molmil
Cryo-EM informed directed evolution of Nitrilase 4 leads to a change in quaternary structure.
分子名称: Bifunctional nitrilase/nitrile hydratase NIT4
著者Mulelu, A.E, Woodward, J.D.
登録日2018-11-14
公開日2019-11-20
最終更新日2024-05-15
実験手法ELECTRON MICROSCOPY (3.9 Å)
主引用文献Cryo-EM informed directed evolution of nitrilase 4 leads to a change in quaternary structure.
To Be Published
8F7Z
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BU of 8f7z by Molmil
VRC34.01_mm28 bound to fusion peptide
分子名称: HIV-1 Env Fusion Peptide, VRC34_m228 Light Chain, VRC34_mm28 Heavy Chain
著者Olia, A.S, Kwong, P.D.
登録日2022-11-21
公開日2023-11-01
最終更新日2024-02-14
実験手法X-RAY DIFFRACTION (2.7 Å)
主引用文献Antibody-directed evolution reveals a mechanism for enhanced neutralization at the HIV-1 fusion peptide site.
Nat Commun, 14, 2023
6I5U
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BU of 6i5u by Molmil
Cryo-EM informed directed evolution of Nitrilase 4 leads to a change in quaternary structure.
分子名称: Bifunctional nitrilase/nitrile hydratase NIT4
著者Mulelu, A.E, Woodward, J.D.
登録日2018-11-14
公開日2019-11-20
最終更新日2024-05-15
実験手法ELECTRON MICROSCOPY (3.9 Å)
主引用文献Cryo-EM informed directed evolution of nitrilase 4 leads to a change in quaternary structure.
To Be Published
6K3D
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BU of 6k3d by Molmil
Structure of multicopper oxidase mutant
分子名称: COPPER (II) ION, CU-O-CU LINKAGE, Multicopper oxidase
著者Sakuraba, H, Ohshida, T, Satomura, T, Yoneda, K, Ohshima, T.
登録日2019-05-17
公開日2020-05-20
最終更新日2023-11-22
実験手法X-RAY DIFFRACTION (1.919 Å)
主引用文献Activity enhancement of multicopper oxidase from a hyperthermophile via directed evolution, and its application as the element of a high performance biocathode.
J.Biotechnol., 325, 2021
3QA9
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BU of 3qa9 by Molmil
Crystal Structure of Prb (PH1109 protein redesigned for binding)
分子名称: CoA binding domain protein
著者Spiegel, P.C.
登録日2011-01-10
公開日2011-04-20
最終更新日2024-02-21
実験手法X-RAY DIFFRACTION (1.9 Å)
主引用文献A de novo protein binding pair by computational design and directed evolution.
Mol.Cell, 42, 2011
3A4H
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BU of 3a4h by Molmil
Structure of cytochrome P450 vdh from Pseudonocardia autotrophica (orthorhombic crystal form)
分子名称: CALCIUM ION, PROTOPORPHYRIN IX CONTAINING FE, Vitamin D hydroxylase
著者Yasutake, Y, Fujii, Y, Cheon, W.K, Arisawa, A, Tamura, T.
登録日2009-07-07
公開日2010-07-14
最終更新日2023-11-01
実験手法X-RAY DIFFRACTION (3.06 Å)
主引用文献Structural evidence for enhancement of sequential vitamin D3 hydroxylation activities by directed evolution of cytochrome P450 vitamin D3 hydroxylase
J.Biol.Chem., 285, 2010
4DU2
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BU of 4du2 by Molmil
cytochrome P450 BM3h-B7 MRI sensor bound to dopamine
分子名称: L-DOPAMINE, PROTOPORPHYRIN IX CONTAINING FE, cytochrome P450 BM3 variant B7
著者Brustad, E.M, Lelyveld, V.S, Snow, C.D, Crook, N, Martinez, F.M, Scholl, T.J, Jasanoff, A, Arnold, F.H.
登録日2012-02-21
公開日2012-06-13
最終更新日2023-09-13
実験手法X-RAY DIFFRACTION (1.9 Å)
主引用文献Structure-guided directed evolution of highly selective p450-based magnetic resonance imaging sensors for dopamine and serotonin.
J.Mol.Biol., 422, 2012
2DCY
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BU of 2dcy by Molmil
Crystal structure of Bacillus subtilis family-11 xylanase
分子名称: 1,4-DIETHYLENE DIOXIDE, D(-)-TARTARIC ACID, Endo-1,4-beta-xylanase A, ...
著者Kondo, H, Miyazaki, K, Takenouchi, M, Noro, N, Suzuki, M, Tsuda, S.
登録日2006-01-18
公開日2006-02-07
最終更新日2023-10-25
実験手法X-RAY DIFFRACTION (1.4 Å)
主引用文献Thermal Stabilization of Bacillus subtilis Family-11 Xylanase by Directed Evolution
J.Biol.Chem., 281, 2006
1ZJ5
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BU of 1zj5 by Molmil
Crystal Structure Analysis of the dienelactone hydrolase mutant (E36D, C123S, A134S, S208G, A229V, K234R) bound with the PMS moiety of the protease inhibitor, Phenylmethylsulfonyl fluoride (PMSF)- 1.7 A
分子名称: Carboxymethylenebutenolidase, GLYCEROL, SULFATE ION
著者Kim, H.-K, Liu, J.-W, Carr, P.D, Ollis, D.L.
登録日2005-04-28
公開日2005-07-05
最終更新日2023-10-25
実験手法X-RAY DIFFRACTION (1.7 Å)
主引用文献Following directed evolution with crystallography: structural changes observed in changing the substrate specificity of dienelactone hydrolase.
Acta Crystallogr.,Sect.D, 61, 2005
1ZIC
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BU of 1zic by Molmil
Crystal Structure Analysis of the dienelactone hydrolase (C123S, R206A) mutant- 1.7 A
分子名称: Carboxymethylenebutenolidase, GLYCEROL, SULFATE ION
著者Kim, H.-K, Liu, J.-W, Carr, P.D, Ollis, D.L.
登録日2005-04-27
公開日2005-07-05
最終更新日2023-10-25
実験手法X-RAY DIFFRACTION (1.7 Å)
主引用文献Following directed evolution with crystallography: structural changes observed in changing the substrate specificity of dienelactone hydrolase.
Acta Crystallogr.,Sect.D, 61, 2005
1ZIX
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BU of 1zix by Molmil
Crystal Structure Analysis of the dienelactone hydrolase mutant (E36D, R105H, C123S, G211D, K234N)- 1.8 A
分子名称: Carboxymethylenebutenolidase, GLYCEROL
著者Kim, H.-K, Liu, J.-W, Carr, P.D, Ollis, D.L.
登録日2005-04-27
公開日2005-07-05
最終更新日2023-10-25
実験手法X-RAY DIFFRACTION (1.8 Å)
主引用文献Following directed evolution with crystallography: structural changes observed in changing the substrate specificity of dienelactone hydrolase.
Acta Crystallogr.,Sect.D, 61, 2005
1ZIY
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BU of 1ziy by Molmil
Crystal Structure Analysis of the dienelactone hydrolase mutant (C123S) bound with the PMS moiety of the protease inhibitor, Phenylmethylsulfonyl fluoride (PMSF)- 1.9 A
分子名称: Carboxymethylenebutenolidase, GLYCEROL, SULFATE ION
著者Kim, H.-K, Liu, J.-W, Carr, P.D, Ollis, D.L.
登録日2005-04-27
公開日2005-07-05
最終更新日2023-10-25
実験手法X-RAY DIFFRACTION (1.9 Å)
主引用文献Following directed evolution with crystallography: structural changes observed in changing the substrate specificity of dienelactone hydrolase.
Acta Crystallogr.,Sect.D, 61, 2005
1ZI6
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BU of 1zi6 by Molmil
Crystal Structure Analysis of the dienelactone hydrolase (C123S) mutant- 1.7 A
分子名称: Carboxymethylenebutenolidase, GLYCEROL, SULFATE ION
著者Kim, H.-K, Liu, J.-W, Carr, P.D, Ollis, D.L.
登録日2005-04-27
公開日2005-07-05
最終更新日2023-10-25
実験手法X-RAY DIFFRACTION (1.7 Å)
主引用文献Following directed evolution with crystallography: structural changes observed in changing the substrate specificity of dienelactone hydrolase.
Acta Crystallogr.,Sect.D, 61, 2005
1ZI9
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BU of 1zi9 by Molmil
Crystal Structure Analysis of the dienelactone hydrolase (E36D, C123S) mutant- 1.5 A
分子名称: Carboxymethylenebutenolidase, GLYCEROL, SULFATE ION
著者Kim, H.-K, Liu, J.-W, Carr, P.D, Ollis, D.L.
登録日2005-04-27
公開日2005-07-05
最終更新日2023-10-25
実験手法X-RAY DIFFRACTION (1.5 Å)
主引用文献Following directed evolution with crystallography: structural changes observed in changing the substrate specificity of dienelactone hydrolase.
Acta Crystallogr.,Sect.D, 61, 2005
1ZJ4
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BU of 1zj4 by Molmil
Crystal Structure Analysis of the dienelactone hydrolase mutant (E36D, C123S) bound with the PMS moiety of the protease inhibitor, Phenylmethylsulfonyl fluoride (PMSF)- 1.7 A
分子名称: Carboxymethylenebutenolidase, GLYCEROL, SULFATE ION
著者Kim, H.-K, Liu, J.-W, Carr, P.D, Ollis, D.L.
登録日2005-04-28
公開日2005-07-05
最終更新日2023-10-25
実験手法X-RAY DIFFRACTION (1.7 Å)
主引用文献Following directed evolution with crystallography: structural changes observed in changing the substrate specificity of dienelactone hydrolase.
Acta Crystallogr.,Sect.D, 61, 2005
1ZI8
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BU of 1zi8 by Molmil
Crystal Structure Analysis of the dienelactone hydrolase mutant(E36D, C123S, A134S, S208G, A229V, K234R)- 1.4 A
分子名称: Carboxymethylenebutenolidase, GLYCEROL, SULFATE ION
著者Kim, H.-K, Liu, J.-W, Carr, P.D, Ollis, D.L.
登録日2005-04-27
公開日2005-07-05
最終更新日2023-10-25
実験手法X-RAY DIFFRACTION (1.4 Å)
主引用文献Following directed evolution with crystallography: structural changes observed in changing the substrate specificity of dienelactone hydrolase.
Acta Crystallogr.,Sect.D, 61, 2005

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