6YXU
 
 | | Structure of Mycobacterium smegmatis HelD protein in complex with RNA polymerase core - State I, primary channel engaged | | Descriptor: | DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', ... | | Authors: | Kouba, T, Koval, T, Krasny, L, Dohnalek, J. | | Deposit date: | 2020-05-03 | | Release date: | 2020-11-04 | | Last modified: | 2024-05-22 | | Method: | ELECTRON MICROSCOPY (3.08 Å) | | Cite: | Mycobacterial HelD is a nucleic acids-clearing factor for RNA polymerase.
Nat Commun, 11, 2020
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6YYS
 | | Structure of Mycobacterium smegmatis HelD protein in complex with RNA polymerase core - State II, primary channel engaged and active site interfering | | Descriptor: | DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', ... | | Authors: | Kouba, T, Koval, T, Krasny, L, Dohnalek, J. | | Deposit date: | 2020-05-05 | | Release date: | 2020-11-04 | | Last modified: | 2024-07-10 | | Method: | ELECTRON MICROSCOPY (3.08 Å) | | Cite: | Mycobacterial HelD is a nucleic acids-clearing factor for RNA polymerase.
Nat Commun, 11, 2020
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6Z11
 | | Structure of Mycobacterium smegmatis HelD protein in complex with RNA polymerase core - State III, primary channel dis-engaged and active site interfering | | Descriptor: | DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', ... | | Authors: | Kouba, T, Koval, T, Krasny, L, Dohnalek, J. | | Deposit date: | 2020-05-11 | | Release date: | 2020-11-04 | | Last modified: | 2024-05-22 | | Method: | ELECTRON MICROSCOPY (3.36 Å) | | Cite: | HelD, a helicase-like protein from gram-positive bacteria
in complex with RNA polymerase
To Be Published
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6EYD
 | | Structure of Mycobacterium smegmatis RNA polymerase Sigma-A holoenzyme | | Descriptor: | DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', ... | | Authors: | Kouba, T, Barvik, I, Krasny, L. | | Deposit date: | 2017-11-11 | | Release date: | 2018-12-12 | | Last modified: | 2024-05-15 | | Method: | ELECTRON MICROSCOPY (4.22 Å) | | Cite: | The Core and Holoenzyme Forms of RNA Polymerase fromMycobacterium smegmatis.
J. Bacteriol., 201, 2019
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6F6W
 | | Structure of Mycobacterium smegmatis RNA polymerase core | | Descriptor: | DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', ... | | Authors: | Kouba, T, Barvik, I, Krasny, L. | | Deposit date: | 2017-12-06 | | Release date: | 2018-12-19 | | Last modified: | 2024-05-15 | | Method: | ELECTRON MICROSCOPY (3.81 Å) | | Cite: | The Core and Holoenzyme Forms of RNA Polymerase fromMycobacterium smegmatis.
J. Bacteriol., 201, 2019
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7OE7
 | | Apo-structure of Lassa virus L protein (well-resolved alpha ribbon) [APO-RIBBON] | | Descriptor: | MAGNESIUM ION, RNA-directed RNA polymerase L, ZINC ION | | Authors: | Kouba, T, Vogel, D, Thorkelsson, S, Quemin, E, Williams, H.M, Milewski, M, Busch, C, Gunther, S, Grunewald, K, Rosenthal, M, Cusack, S. | | Deposit date: | 2021-05-01 | | Release date: | 2021-12-01 | | Last modified: | 2024-05-01 | | Method: | ELECTRON MICROSCOPY (3.73 Å) | | Cite: | Conformational changes in Lassa virus L protein associated with promoter binding and RNA synthesis activity.
Nat Commun, 12, 2021
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7OEA
 | | Lassa virus L protein bound to 3' promoter RNA (well-resolved polymerase core) [3END-CORE] | | Descriptor: | 3' vRNA, MAGNESIUM ION, RNA-directed RNA polymerase L, ... | | Authors: | Kouba, T, Vogel, D, Thorkelsson, S, Quemin, E, Williams, H.M, Milewski, M, Busch, C, Gunther, S, Grunewald, K, Rosenthal, M, Cusack, S. | | Deposit date: | 2021-05-02 | | Release date: | 2021-12-01 | | Last modified: | 2024-05-01 | | Method: | ELECTRON MICROSCOPY (2.7 Å) | | Cite: | Conformational changes in Lassa virus L protein associated with promoter binding and RNA synthesis activity.
Nat Commun, 12, 2021
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7OCH
 | | Apo-structure of Lassa virus L protein (well-resolved polymerase core) [APO-CORE] | | Descriptor: | MAGNESIUM ION, RNA-directed RNA polymerase L, ZINC ION | | Authors: | Kouba, T, Vogel, D, Thorkelsson, S, Quemin, E, Williams, H.M, Milewski, M, Busch, C, Gunther, S, Grunewald, K, Rosenthal, M, Cusack, S. | | Deposit date: | 2021-04-26 | | Release date: | 2021-12-01 | | Last modified: | 2024-05-01 | | Method: | ELECTRON MICROSCOPY (3.14 Å) | | Cite: | Conformational changes in Lassa virus L protein associated with promoter binding and RNA synthesis activity.
Nat Commun, 12, 2021
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7OE3
 | | Apo-structure of Lassa virus L protein (well-resolved endonuclease) [APO-ENDO] | | Descriptor: | MAGNESIUM ION, RNA-directed RNA polymerase L, ZINC ION | | Authors: | Kouba, T, Vogel, D, Thorkelsson, S, Quemin, E, Williams, H.M, Milewski, M, Busch, C, Gunther, S, Grunewald, K, Rosenthal, M, Cusack, S. | | Deposit date: | 2021-05-01 | | Release date: | 2021-12-01 | | Last modified: | 2024-05-01 | | Method: | ELECTRON MICROSCOPY (3.35 Å) | | Cite: | Conformational changes in Lassa virus L protein associated with promoter binding and RNA synthesis activity.
Nat Commun, 12, 2021
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7OEB
 | | Lassa virus L protein bound to 3' promoter RNA (well-resolved endonuclease) [3END-ENDO] | | Descriptor: | 3' vRNA, MAGNESIUM ION, RNA-directed RNA polymerase L, ... | | Authors: | Kouba, T, Vogel, D, Thorkelsson, S, Quemin, E, Williams, H.M, Milewski, M, Busch, C, Gunther, S, Grunewald, K, Rosenthal, M, Cusack, S. | | Deposit date: | 2021-05-02 | | Release date: | 2021-12-01 | | Last modified: | 2024-05-01 | | Method: | ELECTRON MICROSCOPY (3.04 Å) | | Cite: | Conformational changes in Lassa virus L protein associated with promoter binding and RNA synthesis activity.
Nat Commun, 12, 2021
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7OJK
 | | Lassa virus L protein bound to the distal promoter duplex [DISTAL-PROMOTER] | | Descriptor: | 3' RNA, 5' RNA, RNA-directed RNA polymerase L, ... | | Authors: | Kouba, T, Vogel, D, Thorkelsson, S, Quemin, E, Williams, H.M, Milewski, M, Busch, C, Gunther, S, Grunewald, K, Rosenthal, M, Cusack, S. | | Deposit date: | 2021-05-16 | | Release date: | 2021-12-01 | | Last modified: | 2024-05-01 | | Method: | ELECTRON MICROSCOPY (3.89 Å) | | Cite: | Conformational changes in Lassa virus L protein associated with promoter binding and RNA synthesis activity.
Nat Commun, 12, 2021
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7OJL
 | | Lassa virus L protein in a pre-initiation conformation [PREINITIATION] | | Descriptor: | 3' RNA, 5' RNA, MANGANESE (II) ION, ... | | Authors: | Kouba, T, Vogel, D, Thorkelsson, S, Quemin, E, Williams, H.M, Milewski, M, Busch, C, Gunther, S, Grunewald, K, Rosenthal, M, Cusack, S. | | Deposit date: | 2021-05-16 | | Release date: | 2021-12-01 | | Last modified: | 2024-05-01 | | Method: | ELECTRON MICROSCOPY (3.3 Å) | | Cite: | Conformational changes in Lassa virus L protein associated with promoter binding and RNA synthesis activity.
Nat Commun, 12, 2021
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7OJN
 | | Lassa virus L protein in an elongation conformation [ELONGATION] | | Descriptor: | 3' RNA, 5' RNA, 5'-O-[(S)-hydroxy{[(S)-hydroxy(phosphonooxy)phosphoryl]amino}phosphoryl]uridine, ... | | Authors: | Kouba, T, Vogel, D, Thorkelsson, S, Quemin, E, Williams, H.M, Milewski, M, Busch, C, Gunther, S, Grunewald, K, Rosenthal, M, Cusack, S. | | Deposit date: | 2021-05-16 | | Release date: | 2021-12-01 | | Last modified: | 2024-05-01 | | Method: | ELECTRON MICROSCOPY (2.92 Å) | | Cite: | Conformational changes in Lassa virus L protein associated with promoter binding and RNA synthesis activity.
Nat Commun, 12, 2021
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7OJJ
 | | Lassa virus L protein with endonuclease and C-terminal domains in close proximity [MID-LINK] | | Descriptor: | MAGNESIUM ION, RNA-directed RNA polymerase L, ZINC ION | | Authors: | Kouba, T, Vogel, D, Thorkelsson, S, Quemin, E, Williams, H.M, Milewski, M, Busch, C, Gunther, S, Grunewald, K, Rosenthal, M, Cusack, S. | | Deposit date: | 2021-05-16 | | Release date: | 2021-12-01 | | Last modified: | 2024-05-01 | | Method: | ELECTRON MICROSCOPY (3.5 Å) | | Cite: | Conformational changes in Lassa virus L protein associated with promoter binding and RNA synthesis activity.
Nat Commun, 12, 2021
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8CBQ
 | | structure of LEDGF/p75 PWWP domain bound to the H3K36 trimethylated dinucleosome | | Descriptor: | Histone H2A, Histone H2B 1.1, Histone H3, ... | | Authors: | Koutna, E, Kouba, T, Novacek, J, Veverka, V. | | Deposit date: | 2023-01-25 | | Release date: | 2023-09-06 | | Last modified: | 2023-11-15 | | Method: | ELECTRON MICROSCOPY (4 Å) | | Cite: | Multivalency of nucleosome recognition by LEDGF.
Nucleic Acids Res., 51, 2023
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7R0E
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7R1F
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8PEP
 | | H3K36me2 nucleosome-LEDGF/p75 PWWP domain complex - pose 2 | | Descriptor: | Histone H2A, Histone H2B 1.1, Histone H3, ... | | Authors: | Koutna, E, Kouba, T, Veverka, V. | | Deposit date: | 2023-06-14 | | Release date: | 2023-08-16 | | Last modified: | 2023-11-15 | | Method: | ELECTRON MICROSCOPY (3.33 Å) | | Cite: | Multivalency of nucleosome recognition by LEDGF.
Nucleic Acids Res., 51, 2023
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8PEO
 | | H3K36me2 nucleosome-LEDGF/p75 PWWP domain complex | | Descriptor: | (2R)-2-amino-3-(2-dimethylaminoethylsulfanyl)propanoic acid, Histone H2A, Histone H2B 1.1, ... | | Authors: | Koutna, E, Kouba, T, Veverka, V. | | Deposit date: | 2023-06-14 | | Release date: | 2023-08-16 | | Last modified: | 2023-11-15 | | Method: | ELECTRON MICROSCOPY (2.69 Å) | | Cite: | Multivalency of nucleosome recognition by LEDGF.
Nucleic Acids Res., 51, 2023
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8CBN
 | | structure of LEDGF/p75 PWWP domain bound to the H3K36 trimethylated dinucleosome | | Descriptor: | Histone H2A, Histone H2B 1.1, Histone H3, ... | | Authors: | Koutna, E, Kouba, T, Novacek, J, Veverka, V. | | Deposit date: | 2023-01-25 | | Release date: | 2023-12-27 | | Method: | ELECTRON MICROSCOPY (3.34 Å) | | Cite: | Multivalency of nucleosome recognition by LEDGF.
Nucleic Acids Res., 51, 2023
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7QTL
 | | Influenza A/H7N9 polymerase elongation complex | | Descriptor: | 3' vRNA, 5' vRNA, 5'-O-[(S)-hydroxy{[(S)-hydroxy(phosphonooxy)phosphoryl]methyl}phosphoryl]cytidine, ... | | Authors: | Cusack, S, Kouba, T. | | Deposit date: | 2022-01-14 | | Release date: | 2022-12-28 | | Last modified: | 2024-07-17 | | Method: | ELECTRON MICROSCOPY (2.48 Å) | | Cite: | Direct observation of backtracking by influenza A and B polymerases upon consecutive incorporation of the nucleoside analog T1106.
Cell Rep, 42, 2023
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5NP0
 | | Closed dimer of human ATM (Ataxia telangiectasia mutated) | | Descriptor: | Serine-protein kinase ATM | | Authors: | Baretic, D, Pollard, H.K, Fisher, D.I, Johnson, C.M, Santhanam, B, Truman, C.M, Kouba, T, Fersht, A.R, Phillips, C, Williams, R.L. | | Deposit date: | 2017-04-13 | | Release date: | 2017-05-17 | | Last modified: | 2024-05-15 | | Method: | ELECTRON MICROSCOPY (5.7 Å) | | Cite: | Structures of closed and open conformations of dimeric human ATM.
Sci Adv, 3, 2017
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5NP1
 | | Open protomer of human ATM (Ataxia telangiectasia mutated) | | Descriptor: | Serine-protein kinase ATM | | Authors: | Baretic, D, Pollard, H.K, Fisher, D.I, Johnson, C.M, Santhanam, B, Truman, C.M, Kouba, T, Fersht, A.R, Phillips, C, Williams, R.L. | | Deposit date: | 2017-04-13 | | Release date: | 2017-05-17 | | Last modified: | 2024-05-15 | | Method: | ELECTRON MICROSCOPY (5.7 Å) | | Cite: | Structures of closed and open conformations of dimeric human ATM.
Sci Adv, 3, 2017
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8Q3I
 | | Mycobacterium smegmatis RNA polymerase in complex with HelD, SigA and RbpA in State I | | Descriptor: | DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', ... | | Authors: | Koval, T, Krasny, L, Dohnalek, J, Kouba, T. | | Deposit date: | 2023-08-04 | | Release date: | 2024-08-14 | | Method: | ELECTRON MICROSCOPY (3.11 Å) | | Cite: | Molecular insights into RNA polymerase recycling by mycobacterial HelD
To Be Published
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6QCS
 | | Influenza B polymerase pre-initiation complex | | Descriptor: | 3 end, 5 end, Capped RNA, ... | | Authors: | Cusack, S, Kouba, T. | | Deposit date: | 2018-12-30 | | Release date: | 2019-06-05 | | Last modified: | 2024-05-15 | | Method: | ELECTRON MICROSCOPY (3.1 Å) | | Cite: | Structural snapshots of actively transcribing influenza polymerase.
Nat.Struct.Mol.Biol., 26, 2019
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