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PDB: 36 results

7ROF
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BU of 7rof by Molmil
Engineered tryptophan synthase b-subunit from Pyrococcus furiosus, PfTrpB2B9-H275E with L-Trp non-covalently bound
Descriptor: SODIUM ION, TRYPTOPHAN, Tryptophan synthase beta chain 1
Authors:Higgins, P.M, Buller, A.R.
Deposit date:2021-07-30
Release date:2022-08-03
Last modified:2023-11-15
Method:X-RAY DIFFRACTION (2.39 Å)
Cite:Substrate multiplexed protein engineering facilitates promiscuous biocatalytic synthesis.
Nat Commun, 13, 2022
8SF4
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BU of 8sf4 by Molmil
Cystathionine gamma lyase from Thermobifida fusca in internal aldimine form
Descriptor: Cystathionine gamma-lyase, DI(HYDROXYETHYL)ETHER, MAGNESIUM ION, ...
Authors:Perkins, L.J, Buller, A.R, Bindman, C.A.
Deposit date:2023-04-10
Release date:2024-07-03
Method:X-RAY DIFFRACTION (1.455 Å)
Cite:Multiplexed deconvolution of enzyme function in a PLP-dependent protein family
To Be Published
7N2T
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BU of 7n2t by Molmil
O-acetylserine sulfhydrylase from Citrullus vulgaris in the internal aldimine state, with citrate bound
Descriptor: CITRIC ACID, Cysteine synthase, PENTAETHYLENE GLYCOL, ...
Authors:Smith, J.L, Buller, A.R, Bingman, C.A.
Deposit date:2021-05-29
Release date:2022-07-06
Last modified:2024-04-03
Method:X-RAY DIFFRACTION (1.55 Å)
Cite:Investigation of beta-Substitution Activity of O-Acetylserine Sulfhydrolase from Citrullus vulgaris.
Chembiochem, 23, 2022
7MKV
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BU of 7mkv by Molmil
Engineered PLP-dependent decarboxylative aldolase from Aspergillus flavus, UstD2.0, bound as the internal aldimine
Descriptor: Cysteine desulfurase-like protein ustD
Authors:Ellis, J.M, Buller, A.R, Bingman, C.A.
Deposit date:2021-04-27
Release date:2022-05-04
Method:X-RAY DIFFRACTION (2.25 Å)
Cite:Biocatalytic synthesis of non-standard amino acids by a decarboxylative aldol reaction
Nat Catal, 5, 2022
3S3U
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BU of 3s3u by Molmil
Crystal Structure of Uncleaved ThnT T282C
Descriptor: cysteine transferase
Authors:Schildbach, J.F, Wright, N.T, Buller, A.R.
Deposit date:2011-05-18
Release date:2012-02-01
Last modified:2024-02-28
Method:X-RAY DIFFRACTION (1.6 Å)
Cite:Insights into cis-autoproteolysis reveal a reactive state formed through conformational rearrangement.
Proc.Natl.Acad.Sci.USA, 109, 2012
3TM2
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BU of 3tm2 by Molmil
Crystal structure of mature ThnT with a covalently bound product mimic
Descriptor: (2R)-N-(4-chloro-3-oxobutyl)-2,4-dihydroxy-3,3-dimethylbutanamide, cysteine transferase
Authors:Schildbach, J.F, Wright, N.T, Buller, A.R.
Deposit date:2011-08-30
Release date:2012-07-04
Last modified:2023-09-13
Method:X-RAY DIFFRACTION (2 Å)
Cite:Autoproteolytic Activation of ThnT Results in Structural Reorganization Necessary for Substrate Binding and Catalysis.
J.Mol.Biol., 422, 2012
3TM1
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BU of 3tm1 by Molmil
Crystal structure of mature ThnT, a pantetheine hydrolase
Descriptor: GLYCEROL, cysteine transferase
Authors:Schildbach, J.F, Wright, N.T, Buller, A.R.
Deposit date:2011-08-30
Release date:2012-07-04
Last modified:2024-02-28
Method:X-RAY DIFFRACTION (1.8 Å)
Cite:Autoproteolytic Activation of ThnT Results in Structural Reorganization Necessary for Substrate Binding and Catalysis.
J.Mol.Biol., 422, 2012
5E4R
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BU of 5e4r by Molmil
Crystal structure of domain-duplicated synthetic class II ketol-acid reductoisomerase 2Ia_KARI-DD
Descriptor: 1,2-ETHANEDIOL, GLYCEROL, Ketol-acid reductoisomerase, ...
Authors:Cahn, J.K.B, Brinkmann-Chen, S, Buller, A.R, Arnold, F.H.
Deposit date:2015-10-07
Release date:2015-12-23
Last modified:2023-09-27
Method:X-RAY DIFFRACTION (1.94 Å)
Cite:Artificial domain duplication replicates evolutionary history of ketol-acid reductoisomerases.
Protein Sci., 25, 2016
6CUZ
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BU of 6cuz by Molmil
Engineered TrpB from Pyrococcus furiosus, PfTrpB7E6 with (2S,3R)-ethylserine bound as the amino-acrylate
Descriptor: (2E)-2-[(E)-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene)amino]pent-2-enoic acid, PHOSPHATE ION, SODIUM ION, ...
Authors:Scheele, R.A, Buller, A.R, Boville, C.E, Arnold, F.H.
Deposit date:2018-03-27
Release date:2018-09-26
Last modified:2023-11-15
Method:X-RAY DIFFRACTION (1.75 Å)
Cite:Engineered Biosynthesis of beta-Alkyl Tryptophan Analogues.
Angew. Chem. Int. Ed. Engl., 57, 2018
6CUT
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BU of 6cut by Molmil
Engineered Holo TrpB from Pyrococcus furiosus, PfTrpB7E6 with (2S,3S)-isopropylserine bound as the external aldimine
Descriptor: (2S,3S)-3-hydroxy-2-[(E)-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene)amino]-4-methylpentanoic acid (non-preferred name), SODIUM ION, Tryptophan synthase beta chain 1
Authors:Boville, C.E, Scheele, R.A, Buller, A.R, Arnold, F.H.
Deposit date:2018-03-26
Release date:2018-09-26
Last modified:2024-03-13
Method:X-RAY DIFFRACTION (1.77 Å)
Cite:Engineered Biosynthesis of beta-Alkyl Tryptophan Analogues.
Angew. Chem. Int. Ed. Engl., 57, 2018
5UCW
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BU of 5ucw by Molmil
Cytochrome P411 P-4 A82L A78V F263L amination catalyst
Descriptor: NADPH-cytochrome P450 reductase 102A1V3, PROTOPORPHYRIN IX CONTAINING FE
Authors:Zhang, R.K, Buller, A.R, Arnold, F.H.
Deposit date:2016-12-22
Release date:2017-05-17
Last modified:2023-10-04
Method:X-RAY DIFFRACTION (1.7 Å)
Cite:Enantioselective, intermolecular benzylic C-H amination catalysed by an engineered iron-haem enzyme.
Nat Chem, 9, 2017
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