6XVI
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6XUX
| Crystal structure of Megabody Mb-Nb207-cYgjK_NO | Descriptor: | CALCIUM ION, Nanobody,Glucosidase YgjK,Glucosidase YgjK,Nanobody | Authors: | Steyaert, J, Uchanski, T, Fischer, B. | Deposit date: | 2020-01-21 | Release date: | 2021-01-13 | Last modified: | 2024-01-24 | Method: | X-RAY DIFFRACTION (1.90000641 Å) | Cite: | Megabodies expand the nanobody toolkit for protein structure determination by single-particle cryo-EM. Nat.Methods, 18, 2021
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6XV8
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1LRA
| CRYSTALLOGRAPHIC STUDY OF GLU 58 ALA RNASE T1(ASTERISK)2'-GUANOSINE MONOPHOSPHATE AT 1.9 ANGSTROMS RESOLUTION | Descriptor: | GUANOSINE-2'-MONOPHOSPHATE, RIBONUCLEASE T1, SODIUM ION | Authors: | Pletinckx, J, Steyaert, J, Choe, H.-W, Heinemann, U, Wyns, L. | Deposit date: | 1993-10-01 | Release date: | 1994-01-31 | Last modified: | 2017-11-29 | Method: | X-RAY DIFFRACTION (1.9 Å) | Cite: | Crystallographic study of Glu58Ala RNase T1 x 2'-guanosine monophosphate at 1.9-A resolution. Biochemistry, 33, 1994
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1YOE
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6QD6
| Molecular scaffolds expand the nanobody toolkit for cryo-EM applications: crystal structure of Mb-cHopQ-Nb207 | Descriptor: | CHLORIDE ION, Mb-cHopQ-Nb207,Outer membrane protein,Mb-cHopQ-Nb207,Outer membrane protein,Mb-cHopQ-Nb207 | Authors: | Uchanski, T, Masiulis, S, Fischer, B, Kalichuk, V, Wohlkonig, A, Zogg, T, Remaut, H, Vranken, W, Aricescu, A.R, Pardon, E, Steyaert, J. | Deposit date: | 2018-12-31 | Release date: | 2019-12-18 | Last modified: | 2021-01-13 | Method: | X-RAY DIFFRACTION (2.84 Å) | Cite: | Megabodies expand the nanobody toolkit for protein structure determination by single-particle cryo-EM Nat.Methods, 18, 2021
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2Q5J
| X-ray structure of phenylpyruvate decarboxylase in complex with 3-deaza-ThDP | Descriptor: | 2-{4-[(4-AMINO-2-METHYLPYRIMIDIN-5-YL)METHYL]-3-METHYLTHIOPHEN-2-YL}ETHYL TRIHYDROGEN DIPHOSPHATE, MAGNESIUM ION, Phenylpyruvate decarboxylase | Authors: | Versees, W, Spaepen, S, Wood, M.D, Leeper, F.J, Vanderleyden, J, Steyaert, J. | Deposit date: | 2007-06-01 | Release date: | 2007-10-23 | Last modified: | 2023-08-30 | Method: | X-RAY DIFFRACTION (3.2 Å) | Cite: | Molecular mechanism of allosteric substrate activation in a thiamine diphosphate-dependent decarboxylase. J.Biol.Chem., 282, 2007
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2Q5O
| X-ray structure of phenylpyruvate decarboxylase in complex with 3-deaza-ThDP and phenylpyruvate | Descriptor: | 2-{4-[(4-AMINO-2-METHYLPYRIMIDIN-5-YL)METHYL]-3-METHYLTHIOPHEN-2-YL}ETHYL TRIHYDROGEN DIPHOSPHATE, 3-PHENYLPYRUVIC ACID, GLYCEROL, ... | Authors: | Versees, W, Spaepen, S, Wood, M.D, Leeper, F.J, Vanderleyden, J, Steyaert, J. | Deposit date: | 2007-06-01 | Release date: | 2007-10-23 | Last modified: | 2023-08-30 | Method: | X-RAY DIFFRACTION (2.15 Å) | Cite: | Molecular mechanism of allosteric substrate activation in a thiamine diphosphate-dependent decarboxylase. J.Biol.Chem., 282, 2007
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2FF1
| Crystal structure of Trypanosoma vivax nucleoside hydrolase soaked with ImmucillinH | Descriptor: | 1,4-DIDEOXY-4-AZA-1-(S)-(9-DEAZAHYPOXANTHIN-9-YL)-D-RIBITOL, CALCIUM ION, IAG-nucleoside hydrolase | Authors: | Versees, W, Barlow, J, Steyaert, J. | Deposit date: | 2005-12-18 | Release date: | 2006-05-23 | Last modified: | 2022-10-05 | Method: | X-RAY DIFFRACTION (2.07 Å) | Cite: | Transition-state Complex of the Purine-specific Nucleoside Hydrolase of T.vivax: Enzyme Conformational Changes and Implications for Catalysis. J.Mol.Biol., 359, 2006
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2FF2
| Crystal structure of Trypanosoma vivax nucleoside hydrolase co-crystallized with ImmucillinH | Descriptor: | 1,4-DIDEOXY-4-AZA-1-(S)-(9-DEAZAHYPOXANTHIN-9-YL)-D-RIBITOL, CALCIUM ION, IAG-nucleoside hydrolase, ... | Authors: | Versees, W, Barlow, J, Steyaert, J. | Deposit date: | 2005-12-18 | Release date: | 2006-05-23 | Last modified: | 2023-09-20 | Method: | X-RAY DIFFRACTION (2.2 Å) | Cite: | Transition-state Complex of the Purine-specific Nucleoside Hydrolase of T.vivax: Enzyme Conformational Changes and Implications for Catalysis. J.Mol.Biol., 359, 2006
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8OI2
| Crystal structure of Alb1 megabody in complex with human serum albumin | Descriptor: | Alb1 Megabody, Albumin | Authors: | De Felice, S, Zoia, G, Romanyuk, Z, Pardon, E, Steyaert, J, Angelini, A, Cendron, L. | Deposit date: | 2023-03-22 | Release date: | 2024-04-03 | Method: | X-RAY DIFFRACTION (3.3 Å) | Cite: | Crystal structure of human serum albumin in complex with megabody reveals unique human and murine cross-reactive binding site. Protein Sci., 33, 2024
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1LOV
| X-ray structure of the E58A mutant of Ribonuclease T1 complexed with 3'-guanosine monophosphate | Descriptor: | CALCIUM ION, GUANOSINE-3'-MONOPHOSPHATE, Guanyl-specific ribonuclease T1 | Authors: | Mignon, P, Steyaert, J, Loris, R, Geerlings, P, Loverix, S. | Deposit date: | 2002-05-07 | Release date: | 2002-08-21 | Last modified: | 2023-08-16 | Method: | X-RAY DIFFRACTION (1.55 Å) | Cite: | A nucleophile activation dyad in ribonucleases. A combined X-ray crystallographic/ab initio quantum chemical study J.Biol.Chem., 277, 2002
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1BIR
| RIBONUCLEASE T1, PHE 100 TO ALA MUTANT COMPLEXED WITH 2' GMP | Descriptor: | CALCIUM ION, GUANOSINE-2'-MONOPHOSPHATE, RIBONUCLEASE T1 | Authors: | Doumen, J, Gonciarz, M, Zegers, I, Loris, R, Wyns, L, Steyaert, J. | Deposit date: | 1996-01-04 | Release date: | 1996-08-17 | Last modified: | 2021-11-03 | Method: | X-RAY DIFFRACTION (1.8 Å) | Cite: | A catalytic function for the structurally conserved residue Phe 100 of ribonuclease T1. Protein Sci., 5, 1996
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2HOH
| RIBONUCLEASE T1 (N9A MUTANT) COMPLEXED WITH 2'GMP | Descriptor: | CALCIUM ION, GUANOSINE-2'-MONOPHOSPHATE, PHOSPHATE ION, ... | Authors: | Langhorst, U, Loris, R, Denisov, V.P, Doumen, J, Roose, P, Maes, D, Halle, B, Steyaert, J. | Deposit date: | 1998-09-14 | Release date: | 1998-09-23 | Last modified: | 2023-08-30 | Method: | X-RAY DIFFRACTION (1.9 Å) | Cite: | Dissection of the structural and functional role of a conserved hydration site in RNase T1. Protein Sci., 8, 1999
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7ZC2
| Dipeptide and tripeptide Permease C (DtpC) | Descriptor: | Amino acid/peptide transporter | Authors: | Killer, M, Finocchio, G, Pardon, E, Steyaert, J, Loew, C. | Deposit date: | 2022-03-25 | Release date: | 2022-07-06 | Last modified: | 2022-08-10 | Method: | ELECTRON MICROSCOPY (2.72 Å) | Cite: | Cryo-EM Structure of an Atypical Proton-Coupled Peptide Transporter: Di- and Tripeptide Permease C. Front Mol Biosci, 9, 2022
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7ZRA
| Crystal structure of E.coli LexA in complex with nanobody NbSOS1(Nb14497) | Descriptor: | 1,2-ETHANEDIOL, LexA repressor, Nanobody NbSOS1 (Nb14497) | Authors: | Maso, L, Vascon, F, Chinellato, M, Pardon, E, Steyaert, J, Angelini, A, Tondi, D, Cendron, L. | Deposit date: | 2022-05-04 | Release date: | 2022-10-26 | Last modified: | 2024-01-31 | Method: | X-RAY DIFFRACTION (2.8 Å) | Cite: | Nanobodies targeting LexA autocleavage disclose a novel suppression strategy of SOS-response pathway. Structure, 30, 2022
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2AAE
| THE ROLE OF HISTIDINE-40 IN RIBONUCLEASE T1 CATALYSIS: THREE-DIMENSIONAL STRUCTURES OF THE PARTIALLY ACTIVE HIS40LYS MUTANT | Descriptor: | CALCIUM ION, PHOSPHATE ION, RIBONUCLEASE T1 | Authors: | Zegers, I, Verhelst, P, Choe, C.W, Steyaert, J, Heinemann, U, Wyns, L, Saenger, W. | Deposit date: | 1992-09-15 | Release date: | 1994-01-31 | Last modified: | 2017-11-29 | Method: | X-RAY DIFFRACTION (1.8 Å) | Cite: | Role of histidine-40 in ribonuclease T1 catalysis: three-dimensionalstructures of the partially active His40Lys mutant. Biochemistry, 31, 1992
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2AAD
| THE ROLE OF HISTIDINE-40 IN RIBONUCLEASE T1 CATALYSIS: THREE-DIMENSIONAL STRUCTURES OF THE PARTIALLY ACTIVE HIS40LYS MUTANT | Descriptor: | CALCIUM ION, GUANOSINE-2'-MONOPHOSPHATE, RIBONUCLEASE T1 ISOZYME | Authors: | Zegers, I, Verhelst, P, Choe, C.W, Steyaert, J, Heinemann, U, Wyns, L, Saenger, W. | Deposit date: | 1992-09-15 | Release date: | 1994-01-31 | Last modified: | 2017-11-29 | Method: | X-RAY DIFFRACTION (2 Å) | Cite: | Role of histidine-40 in ribonuclease T1 catalysis: three-dimensionalstructures of the partially active His40Lys mutant. Biochemistry, 31, 1992
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7PHP
| Structure of Multidrug and Toxin Compound Extrusion (MATE) transporter NorM by NabFab-fiducial assisted cryo-EM | Descriptor: | Anti-Fab nanobody, Multidrug resistance protein NorM, NabFab HC, ... | Authors: | Bloch, J.S, Mukherjee, S, Kowal, J, Niederer, M, Pardon, E, Steyaert, J, Kossiakoff, A.A, Locher, K.P. | Deposit date: | 2021-08-18 | Release date: | 2021-09-01 | Last modified: | 2021-12-01 | Method: | ELECTRON MICROSCOPY (3.47 Å) | Cite: | Development of a universal nanobody-binding Fab module for fiducial-assisted cryo-EM studies of membrane proteins. Proc.Natl.Acad.Sci.USA, 118, 2021
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3P0G
| Structure of a nanobody-stabilized active state of the beta2 adrenoceptor | Descriptor: | 8-[(1R)-2-{[1,1-dimethyl-2-(2-methylphenyl)ethyl]amino}-1-hydroxyethyl]-5-hydroxy-2H-1,4-benzoxazin-3(4H)-one, Beta-2 adrenergic receptor, Lysozyme, ... | Authors: | Rasmussen, S.G.F, Choi, H.-J, Fung, J.J, Pardon, E, Casarosa, P, Chae, P.S, DeVree, B.T, Rosenbaum, D.M, Thian, F.S, Kobilka, T.S, Schnapp, A, Konetzki, I, Sunahara, R.K, Gellman, S.H, Pautsch, A, Steyaert, J, Weis, W.I, Kobilka, B.K. | Deposit date: | 2010-09-28 | Release date: | 2011-01-19 | Last modified: | 2023-09-06 | Method: | X-RAY DIFFRACTION (3.5 Å) | Cite: | Structure of a nanobody-stabilized active state of the b2 adrenoceptor Nature, 469, 2011
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8G8W
| Molecular mechanism of nucleotide inhibition of human uncoupling protein 1 | Descriptor: | CARDIOLIPIN, GUANOSINE-5'-TRIPHOSPHATE, Mitochondrial brown fat uncoupling protein 1, ... | Authors: | Gogoi, P, Jones, S.A, Ruprecht, J.J, King, M.S, Lee, Y, Zogg, T, Pardon, E, Chand, D, Steimle, S, Copeman, D, Cotrim, C.A, Steyaert, J, Crichton, P.G, Moiseenkova-Bell, V, Kunji, E.R.S. | Deposit date: | 2023-02-20 | Release date: | 2023-06-07 | Method: | ELECTRON MICROSCOPY (3.8 Å) | Cite: | Structural basis of purine nucleotide inhibition of human uncoupling protein 1. Sci Adv, 9, 2023
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3ZBI
| Fitting result in the O-layer of the subnanometer structure of the bacterial pKM101 type IV secretion system core complex digested with elastase | Descriptor: | TRAF PROTEIN, TRAN PROTEIN, TRAO PROTEIN | Authors: | Rivera-Calzada, A, Fronzes, R, Savva, C.G, Chandran, V, Lian, P.W, Laeremans, T, Pardon, E, Steyaert, J, Remaut, H, Waksman, G, Orlova, E.V. | Deposit date: | 2012-11-10 | Release date: | 2013-04-03 | Last modified: | 2017-08-23 | Method: | ELECTRON MICROSCOPY (8.5 Å) | Cite: | Structure of a Bacterial Type Iv Secretion Core Complex at Subnanometre Resolution. Embo J., 32, 2013
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3ZBJ
| Fitting results in the I-layer of the subnanometer structure of the bacterial pKM101 type IV secretion system core complex digested with elastase | Descriptor: | TRAO PROTEIN | Authors: | Rivera-Calzada, A, Fronzes, R, Savva, C.G, Chandran, V, Lian, P.W, Laeremans, T, Pardon, E, Steyaert, J, Remaut, H, Waksman, G, Orlova, E.V. | Deposit date: | 2012-11-10 | Release date: | 2013-04-03 | Last modified: | 2017-08-30 | Method: | ELECTRON MICROSCOPY (8.5 Å) | Cite: | Structure of a Bacterial Type Iv Secretion Core Complex at Subnanometre Resolution. Embo J., 32, 2013
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3BIR
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2BU4
| RIBONUCLEASE T1 COMPLEX WITH 2'GMP | Descriptor: | CALCIUM ION, GUANOSINE-2'-MONOPHOSPHATE, PROTEIN (RIBONUCLEASE T1) | Authors: | Loris, R, Devos, S, Langhorst, U, Decanniere, K, Bouckaert, J, Maes, D, Transue, T.R, Steyaert, J. | Deposit date: | 1998-09-14 | Release date: | 1998-09-23 | Last modified: | 2023-08-23 | Method: | X-RAY DIFFRACTION (1.95 Å) | Cite: | Conserved water molecules in a large family of microbial ribonucleases. Proteins, 36, 1999
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