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6XUX

Crystal structure of Megabody Mb-Nb207-cYgjK_NO

Summary for 6XUX
Entry DOI10.2210/pdb6xux/pdb
DescriptorNanobody,Glucosidase YgjK,Glucosidase YgjK,Nanobody, CALCIUM ION (3 entities in total)
Functional Keywordsmegabody, scaffold, structural protein
Biological sourceEscherichia coli K-12
More
Total number of polymer chains1
Total formula weight101659.08
Authors
Steyaert, J.,Uchanski, T.,Fischer, B. (deposition date: 2020-01-21, release date: 2021-01-13, Last modification date: 2024-10-23)
Primary citationUchanski, T.,Masiulis, S.,Fischer, B.,Kalichuk, V.,Lopez-Sanchez, U.,Zarkadas, E.,Weckener, M.,Sente, A.,Ward, P.,Wohlkonig, A.,Zogg, T.,Remaut, H.,Naismith, J.H.,Nury, H.,Vranken, W.,Aricescu, A.R.,Pardon, E.,Steyaert, J.
Megabodies expand the nanobody toolkit for protein structure determination by single-particle cryo-EM.
Nat.Methods, 18:60-68, 2021
Cited by
PubMed Abstract: Nanobodies are popular and versatile tools for structural biology. They have a compact single immunoglobulin domain organization, bind target proteins with high affinities while reducing their conformational heterogeneity and stabilize multi-protein complexes. Here we demonstrate that engineered nanobodies can also help overcome two major obstacles that limit the resolution of single-particle cryo-electron microscopy reconstructions: particle size and preferential orientation at the water-air interfaces. We have developed and characterized constructs, termed megabodies, by grafting nanobodies onto selected protein scaffolds to increase their molecular weight while retaining the full antigen-binding specificity and affinity. We show that the megabody design principles are applicable to different scaffold proteins and recognition domains of compatible geometries and are amenable for efficient selection from yeast display libraries. Moreover, we demonstrate that megabodies can be used to obtain three-dimensional reconstructions for membrane proteins that suffer from severe preferential orientation or are otherwise too small to allow accurate particle alignment.
PubMed: 33408403
DOI: 10.1038/s41592-020-01001-6
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (1.90000643078 Å)
Structure validation

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