7OX9
 
 | | Target-bound SpCas9 complex with AAVS1 all-RNA guide | | Descriptor: | AAVS1 non-target DNA strand, AAVS1 target DNA strand, CRISPR-associated endonuclease Cas9/Csn1, ... | | Authors: | Pacesa, M, Donohoue, P, May, A.P, Jinek, M, Cameron, P. | | Deposit date: | 2021-06-22 | | Release date: | 2021-09-15 | | Last modified: | 2024-01-31 | | Method: | X-RAY DIFFRACTION (2.45 Å) | | Cite: | Conformational control of Cas9 by CRISPR hybrid RNA-DNA guides mitigates off-target activity in T cells.
Mol.Cell, 81, 2021
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6RVZ
 | | Crystal structure of ANGEL2, a 2',3'-cyclic phosphatase, in complex with adenosine-2',3'-vanadate | | Descriptor: | ADENOSINE, MAGNESIUM ION, POTASSIUM ION, ... | | Authors: | Kroupova, A, Jinek, M. | | Deposit date: | 2019-06-03 | | Release date: | 2020-05-20 | | Last modified: | 2024-05-01 | | Method: | X-RAY DIFFRACTION (2.1 Å) | | Cite: | ANGEL2 is a member of the CCR4 family of deadenylases with 2',3'-cyclic phosphatase activity.
Science, 369, 2020
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6QZK
 | | Structure of Clostridium butyricum Argonaute bound to a guide DNA (5' deoxycytidine) and a 19-mer target DNA | | Descriptor: | Clostridium butyricum Argonaute, DNA target (5'-D(T*AP*TP*AP*CP*AP*AP*CP*CP*TP*AP*CP*TP*AP*CP*CP*TP*CP*T)-3'), FORMIC ACID, ... | | Authors: | Swarts, D.C, Jinek, M, Hegge, J.W, Van der Oost, J. | | Deposit date: | 2019-03-11 | | Release date: | 2019-04-24 | | Last modified: | 2024-01-24 | | Method: | X-RAY DIFFRACTION (3.548 Å) | | Cite: | DNA-guided DNA cleavage at moderate temperatures by Clostridium butyricum Argonaute.
Nucleic Acids Res., 47, 2019
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6RW0
 | | Crystal structure of ANGEL2, a 2',3'-cyclic phosphatase | | Descriptor: | GLYCEROL, MAGNESIUM ION, POTASSIUM ION, ... | | Authors: | Kroupova, A, Jinek, M. | | Deposit date: | 2019-06-03 | | Release date: | 2020-05-20 | | Last modified: | 2024-01-24 | | Method: | X-RAY DIFFRACTION (1.45 Å) | | Cite: | ANGEL2 is a member of the CCR4 family of deadenylases with 2',3'-cyclic phosphatase activity.
Science, 369, 2020
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3ICQ
 | | Karyopherin nuclear state | | Descriptor: | Exportin-T, GTP-binding nuclear protein GSP1/CNR1, GUANOSINE-5'-TRIPHOSPHATE, ... | | Authors: | Cook, A.G, Fukuhara, N, Jinek, M, Conti, E. | | Deposit date: | 2009-07-18 | | Release date: | 2009-08-25 | | Last modified: | 2023-11-01 | | Method: | X-RAY DIFFRACTION (3.2 Å) | | Cite: | Structures of the tRNA export factor in the nuclear and cytosolic states
Nature, 461, 2009
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3IBV
 | | Karyopherin cytosolic state | | Descriptor: | CALCIUM ION, Exportin-T | | Authors: | Cook, A.G, Fukuhara, N, Jinek, M, Conti, E. | | Deposit date: | 2009-07-17 | | Release date: | 2009-08-25 | | Last modified: | 2024-03-20 | | Method: | X-RAY DIFFRACTION (3.1 Å) | | Cite: | Structures of the tRNA export factor in the nuclear and cytosolic states
Nature, 461, 2009
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7PLA
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7OXD
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7P3A
 | | N-terminal domain of CGI-99 | | Descriptor: | (4S)-2-METHYL-2,4-PENTANEDIOL, CHLORIDE ION, ISOPROPYL ALCOHOL, ... | | Authors: | Kroupova, A, Jinek, M. | | Deposit date: | 2021-07-07 | | Release date: | 2021-12-22 | | Method: | X-RAY DIFFRACTION (2 Å) | | Cite: | Molecular architecture of the human tRNA ligase complex.
Elife, 10, 2021
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7P3B
 | | Human RNA ligase RTCB in complex with GMP and Co(II) | | Descriptor: | ACETATE ION, COBALT (II) ION, FORMIC ACID, ... | | Authors: | Kroupova, A, Ackle, F, Jinek, M. | | Deposit date: | 2021-07-07 | | Release date: | 2021-12-22 | | Last modified: | 2024-01-31 | | Method: | X-RAY DIFFRACTION (2.3 Å) | | Cite: | Molecular architecture of the human tRNA ligase complex.
Elife, 10, 2021
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7PLH
 | | Scytonema hofmannii TnsC bound to AMPPNP and DNA | | Descriptor: | DNA (5'-D(P*AP*TP*AP*TP*AP*TP*AP*TP*AP*TP*AP*TP*AP*TP*AP*TP*AP*TP*AP*TP*AP*T)-3'), MAGNESIUM ION, PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER, ... | | Authors: | Querques, I, Jinek, M. | | Deposit date: | 2021-08-31 | | Release date: | 2021-12-01 | | Last modified: | 2024-07-17 | | Method: | ELECTRON MICROSCOPY (3.57 Å) | | Cite: | Target site selection and remodelling by type V CRISPR-transposon systems.
Nature, 599, 2021
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5FW1
 | | Crystal structure of SpyCas9 variant VQR bound to sgRNA and TGAG PAM target DNA | | Descriptor: | CRISPR-ASSOCIATED ENDONUCLEASE CAS9/CSN1, MAGNESIUM ION, NON-TARGET DNA STRAND, ... | | Authors: | Anders, C, Bargsten, K, Jinek, M. | | Deposit date: | 2016-02-11 | | Release date: | 2016-04-20 | | Last modified: | 2024-01-10 | | Method: | X-RAY DIFFRACTION (2.499 Å) | | Cite: | Structural Plasticity of Pam Recognition by Engineered Variants of the RNA-Guided Endonuclease Cas9.
Mol.Cell, 61, 2016
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5FW3
 | | Crystal structure of SpCas9 variant VRER bound to sgRNA and TGCG PAM target DNA | | Descriptor: | CRISPR-ASSOCIATED ENDONUCLEASE CAS9/CSN1, MAGNESIUM ION, NON-TARGET DNA STRAND, ... | | Authors: | Anders, C, Bargsten, K, Jinek, M. | | Deposit date: | 2016-02-11 | | Release date: | 2016-06-15 | | Last modified: | 2024-01-10 | | Method: | X-RAY DIFFRACTION (2.7 Å) | | Cite: | Structural Plasticity of Pam Recognition by Engineered Variants of the RNA-Guided Endonuclease Cas9.
Mol.Cell, 61, 2016
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5FSH
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5FW2
 | | Crystal structure of SpCas9 variant EQR bound to sgRNA and TGAG PAM target DNA | | Descriptor: | CRISPR-ASSOCIATED ENDONUCLEASE CAS9/CSN1, MAGNESIUM ION, NON-TARGET DNA STRAND, ... | | Authors: | Anders, C, Bargsten, K, Jinek, M. | | Deposit date: | 2016-02-11 | | Release date: | 2016-06-15 | | Last modified: | 2024-01-10 | | Method: | X-RAY DIFFRACTION (2.676 Å) | | Cite: | Structural Plasticity of Pam Recognition by Engineered Variants of the RNA-Guided Endonuclease Cas9.
Mol.Cell, 61, 2016
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8Q3Z
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8Q3Y
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3P3I
 | | Crystal structure of the F36A mutant of the fluoroacetyl-CoA-specific thioesterase FlK in complex with fluoroacetate and CoA | | Descriptor: | COENZYME A, Fluoroacetyl coenzyme A thioesterase, fluoroacetic acid | | Authors: | Weeks, A.M, Coyle, S.M, Jinek, M, Doudna, J.A, Chang, M.C.Y. | | Deposit date: | 2010-10-04 | | Release date: | 2010-10-20 | | Last modified: | 2011-11-16 | | Method: | X-RAY DIFFRACTION (2 Å) | | Cite: | Structural and biochemical studies of a fluoroacetyl-CoA-specific thioesterase reveal a molecular basis for fluorine selectivity.
Biochemistry, 49, 2010
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8QLP
 | | CryoEM structure of the RNA/DNA bound SPARTA (BabAgo/TIR-APAZ) tetrameric complex | | Descriptor: | DNA (5'-D(*AP*CP*TP*AP*AP*TP*AP*GP*AP*TP*TP*AP*GP*AP*GP*CP*CP*GP*TP*C)-3'), MAGNESIUM ION, RNA (5'-R(*AP*UP*GP*AP*CP*GP*GP*CP*UP*CP*UP*AP*AP*UP*CP*UP*AP*UP*UP*AP*GP*U)-3'), ... | | Authors: | Finocchio, G, Koopal, B, Potocnik, A, Heijstek, C, Jinek, M, Swarts, D. | | Deposit date: | 2023-09-20 | | Release date: | 2024-01-31 | | Last modified: | 2024-03-06 | | Method: | ELECTRON MICROSCOPY (3.14 Å) | | Cite: | Target DNA-dependent activation mechanism of the prokaryotic immune system SPARTA.
Nucleic Acids Res., 52, 2024
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8QLO
 | | CryoEM structure of the apo SPARTA (BabAgo/TIR-APAZ) complex | | Descriptor: | Short prokaryotic Argonaute, Toll/interleukin-1 receptor domain-containing protein | | Authors: | Finocchio, G, Koopal, B, Potocnik, A, Heijstek, C, Jinek, M, Swarts, D. | | Deposit date: | 2023-09-20 | | Release date: | 2024-01-31 | | Last modified: | 2024-03-06 | | Method: | ELECTRON MICROSCOPY (2.57 Å) | | Cite: | Target DNA-dependent activation mechanism of the prokaryotic immune system SPARTA.
Nucleic Acids Res., 52, 2024
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3P2S
 | | Crystal structure of the fluoroacetyl-CoA-specific thioesterase FlK in an open conformation | | Descriptor: | Fluoroacetyl coenzyme A thioesterase | | Authors: | Weeks, A.M, Coyle, S.M, Jinek, M, Doudna, J.A, Chang, M.C.Y. | | Deposit date: | 2010-10-04 | | Release date: | 2010-10-20 | | Last modified: | 2011-11-16 | | Method: | X-RAY DIFFRACTION (1.95 Å) | | Cite: | Structural and biochemical studies of a fluoroacetyl-CoA-specific thioesterase reveal a molecular basis for fluorine selectivity.
Biochemistry, 49, 2010
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8Q40
 | | Crystal structure of cA4 activated Can2 in complex with a cleaved DNA substrate | | Descriptor: | Cyclic tetraadenosine monophosphate (cA4), DNA (5'-D(*TP*CP*A)-3'), DUF1887 family protein, ... | | Authors: | Jungfer, K, Sigg, A, Jinek, M. | | Deposit date: | 2023-08-04 | | Release date: | 2023-11-22 | | Last modified: | 2024-01-24 | | Method: | X-RAY DIFFRACTION (2.21 Å) | | Cite: | Substrate selectivity and catalytic activation of the type III CRISPR ancillary nuclease Can2.
Nucleic Acids Res., 52, 2024
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8Q41
 | | Crystal structure of Can2 (E341A) bound to cA4 and TTTAAA ssDNA | | Descriptor: | Cyclic tetraadenosine monophosphate (cA4), DNA (5'-D(*TP*TP*AP*AP*A)-3'), DUF1887 family protein, ... | | Authors: | Jungfer, K, Sigg, A, Jinek, M. | | Deposit date: | 2023-08-04 | | Release date: | 2023-11-22 | | Last modified: | 2024-01-24 | | Method: | X-RAY DIFFRACTION (2.38 Å) | | Cite: | Substrate selectivity and catalytic activation of the type III CRISPR ancillary nuclease Can2.
Nucleic Acids Res., 52, 2024
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8Q42
 | | Crystal structure of cA4-bound Can2 (E341A) in complex with oligo-A DNA | | Descriptor: | Cyclic tetraadenosine monophosphate (cA4), DNA (5'-D(*AP*AP*AP*A)-3'), DUF1887 family protein, ... | | Authors: | Jungfer, K, Sigg, A, Jinek, M. | | Deposit date: | 2023-08-04 | | Release date: | 2023-11-22 | | Last modified: | 2024-01-24 | | Method: | X-RAY DIFFRACTION (1.97 Å) | | Cite: | Substrate selectivity and catalytic activation of the type III CRISPR ancillary nuclease Can2.
Nucleic Acids Res., 52, 2024
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8Q43
 | | Crystal structure of cA4-bound Can2 (E341A) in complex with oligo-C DNA | | Descriptor: | Cyclic tetraadenosine monophosphate (cA4), DNA (5'-D(*CP*CP*CP*CP*C)-3'), DUF1887 family protein, ... | | Authors: | Jungfer, K, Sigg, A, Jinek, M. | | Deposit date: | 2023-08-04 | | Release date: | 2023-11-22 | | Last modified: | 2024-01-24 | | Method: | X-RAY DIFFRACTION (2.28 Å) | | Cite: | Substrate selectivity and catalytic activation of the type III CRISPR ancillary nuclease Can2.
Nucleic Acids Res., 52, 2024
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