5DWB
 
 | | Crystal structure of specific restriction endonuclease AgeI-DNA complex | | Descriptor: | DNA (5'-D(*TP*TP*CP*GP*AP*CP*CP*GP*GP*TP*CP*GP*A*)-3'), Type-2 restriction enzyme AgeI | | Authors: | Tamulaitiene, G, Jovaisaite, V, Grazulis, S, Siksnys, V. | | Deposit date: | 2015-09-22 | | Release date: | 2016-09-28 | | Last modified: | 2024-01-10 | | Method: | X-RAY DIFFRACTION (2.4 Å) | | Cite: | Restriction endonuclease AgeI is a monomer which dimerizes to cleave DNA.
Nucleic Acids Res., 45, 2017
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5DWA
 | | Crystal structure of pre-specific restriction endonuclease AgeI-DNA complex | | Descriptor: | DNA (5'-D(*TP*CP*GP*AP*CP*CP*GP*GP*TP*CP*G*)-3'), Type-2 restriction enzyme AgeI | | Authors: | Tamulaitiene, G, Jovaisaite, V, Grazulis, S, Siksnys, V. | | Deposit date: | 2015-09-22 | | Release date: | 2016-10-05 | | Last modified: | 2024-05-08 | | Method: | X-RAY DIFFRACTION (1.5 Å) | | Cite: | Restriction endonuclease AgeI is a monomer which dimerizes to cleave DNA.
Nucleic Acids Res., 45, 2017
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5DWC
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5O63
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8BF8
 | | ISDra2 TnpB in complex with reRNA | | Descriptor: | Deinococcus radiodurans R1 chromosome 1, RNA-guided DNA endonuclease TnpB | | Authors: | Sasnauskas, G, Tamulaitiene, G, Carabias, A, Siksnys, V, Montoya, G, Druteika, G, Silanskas, A, Venclovas, C, Karvelis, T, Kazlauskas, D. | | Deposit date: | 2022-10-24 | | Release date: | 2023-04-12 | | Last modified: | 2024-07-24 | | Method: | ELECTRON MICROSCOPY (2.8 Å) | | Cite: | TnpB structure reveals minimal functional core of Cas12 nuclease family.
Nature, 616, 2023
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1CFR
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1D02
 | | CRYSTAL STRUCTURE OF MUNI RESTRICTION ENDONUCLEASE IN COMPLEX WITH COGNATE DNA | | Descriptor: | DNA (5'-D(*GP*CP*CP*AP*AP*TP*TP*GP*GP*C)-3'), TYPE II RESTRICTION ENZYME MUNI | | Authors: | Deibert, M, Grazulis, S, Janulaitis, A, Siksnys, V, Huber, R. | | Deposit date: | 1999-09-08 | | Release date: | 2000-03-08 | | Last modified: | 2024-02-07 | | Method: | X-RAY DIFFRACTION (1.7 Å) | | Cite: | Crystal structure of MunI restriction endonuclease in complex with cognate DNA at 1.7 A resolution.
EMBO J., 18, 1999
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6EKO
 | | Crystal structure of Type IIP restriction endonuclease PfoI with cognate DNA | | Descriptor: | CALCIUM ION, DNA (5'-D(*CP*GP*CP*TP*CP*CP*CP*GP*GP*AP*GP*CP*GP*T)-3'), Restriction endonuclease PfoI | | Authors: | Tamulaitiene, G, Manakova, E, Jovaisaite, V, Grazulis, S, Siksnys, V. | | Deposit date: | 2017-09-26 | | Release date: | 2018-10-10 | | Last modified: | 2019-04-24 | | Method: | X-RAY DIFFRACTION (2.284 Å) | | Cite: | Unique mechanism of target recognition by PfoI restriction endonuclease of the CCGG-family.
Nucleic Acids Res., 47, 2019
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6EK1
 | | Crystal structure of Type IIP restriction endonuclease PfoI | | Descriptor: | 1,2-ETHANEDIOL, restriction endonuclease PfoI | | Authors: | Tamulaitiene, G, Manakova, E, Jovaisaite, V, Grazulis, S, Siksnys, V. | | Deposit date: | 2017-09-25 | | Release date: | 2018-10-10 | | Last modified: | 2024-05-08 | | Method: | X-RAY DIFFRACTION (2.601 Å) | | Cite: | Unique mechanism of target recognition by PfoI restriction endonuclease of the CCGG-family.
Nucleic Acids Res., 47, 2019
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3HQF
 | | Crystal structure of restriction endonuclease EcoRII N-terminal effector-binding domain in complex with cognate DNA | | Descriptor: | 5'-D(*CP*GP*CP*CP*AP*GP*GP*GP*C)-3', 5'-D(*GP*CP*CP*CP*TP*GP*GP*CP*G)-3', Restriction endonuclease | | Authors: | Golovenko, D, Manakova, E, Grazulis, S, Tamulaitiene, G, Siksnys, V. | | Deposit date: | 2009-06-06 | | Release date: | 2009-09-22 | | Last modified: | 2023-09-06 | | Method: | X-RAY DIFFRACTION (2.51 Å) | | Cite: | Structural mechanisms for the 5'-CCWGG sequence recognition by the N- and C-terminal domains of EcoRII.
Nucleic Acids Res., 37, 2009
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3HQG
 | | Crystal structure of restriction endonuclease EcoRII catalytic C-terminal domain in complex with cognate DNA | | Descriptor: | 5'-D(*TP*AP*GP*CP*CP*TP*GP*GP*TP*CP*GP*A)-3', 5'-D(*TP*CP*GP*AP*CP*CP*AP*GP*GP*CP*TP*A)-3', GLYCEROL, ... | | Authors: | Golovenko, D, Manakova, E, Grazulis, S, Tamulaitiene, G, Siksnys, V. | | Deposit date: | 2009-06-06 | | Release date: | 2009-09-22 | | Last modified: | 2023-09-06 | | Method: | X-RAY DIFFRACTION (2.6 Å) | | Cite: | Structural mechanisms for the 5'-CCWGG sequence recognition by the N- and C-terminal domains of EcoRII.
Nucleic Acids Res., 37, 2009
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6EKR
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2VLA
 | | Crystal structure of restriction endonuclease BpuJI recognition domain in complex with cognate DNA | | Descriptor: | 1-(2-METHOXY-ETHOXY)-2-{2-[2-(2-METHOXY-ETHOXY]-ETHOXY}-ETHANE, 5'-D(*GP*GP*TP*AP*CP*CP*CP*GP*TP*GP *GP*A)-3', 5'-D(*TP*CP*CP*AP*CP*GP*GP*GP*TP*AP *CP*C)-3', ... | | Authors: | Sukackaite, R, Grazulis, S, Bochtler, M, Siksnys, V. | | Deposit date: | 2008-01-11 | | Release date: | 2008-05-06 | | Last modified: | 2024-05-08 | | Method: | X-RAY DIFFRACTION (1.3 Å) | | Cite: | The Recognition Domain of the Bpuji Restriction Endonuclease in Complex with Cognate DNA at 1.3-A Resolution.
J.Mol.Biol., 378, 2008
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8J12
 | | Cryo-EM structure of the AsCas12f-sgRNA-target DNA ternary complex | | Descriptor: | DNA (38-MER), MAGNESIUM ION, RNA (247-MER), ... | | Authors: | Hino, T, Omura, N.S, Nakagawa, R, Togashi, T, Takeda, N.S, Hiramoto, T, Tasaka, S, Hirano, H, Tokuyama, T, Uosaki, H, Ishiguro, H, Yamano, H, Ozaki, Y, Motooka, D, Mori, H, Kirita, Y, Kise, Y, Itoh, Y, Matoba, S, Aburatani, H, Yachie, N, Siksnys, V, Ohmori, T, Hoshino, A, Nureki, O. | | Deposit date: | 2023-04-12 | | Release date: | 2023-09-27 | | Method: | ELECTRON MICROSCOPY (3.08 Å) | | Cite: | Minimal and most efficient genome editing Cas enzyme
To Be Published
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8J3R
 | | Cryo-EM structure of the AsCas12f-HKRA-sgRNAS3-5v7-target DNA | | Descriptor: | DNA (37-MER), DNA (38-MER), MAGNESIUM ION, ... | | Authors: | Hino, T, Omura, N.S, Nakagawa, R, Togashi, T, Takeda, N.S, Hiramoto, T, Tasaka, S, Hirano, H, Tokuyama, T, Uosaki, H, Ishiguro, H, Yamano, H, Ozaki, Y, Motooka, D, Mori, H, Kirita, Y, Kise, Y, Itoh, Y, Matoba, S, Aburatani, H, Yachie, N, Siksnys, V, Ohmori, T, Hoshino, A, Nureki, O. | | Deposit date: | 2023-04-18 | | Release date: | 2023-09-27 | | Method: | ELECTRON MICROSCOPY (2.95 Å) | | Cite: | Minimal and most efficient genome editing Cas enzyme
To Be Published
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8J1J
 | | Cryo-EM structure of the AsCas12f-YHAM-sgRNAS3-5v7-target DNA | | Descriptor: | DNA (38-MER), MAGNESIUM ION, RNA (118-MER), ... | | Authors: | Hino, T, Omura, N.S, Nakagawa, R, Togashi, T, Takeda, N.S, Hiramoto, T, Tasaka, S, Hirano, H, Tokuyama, T, Uosaki, H, Ishiguro, H, Yamano, H, Ozaki, Y, Motooka, D, Mori, H, Kirita, Y, Kise, Y, Itoh, Y, Matoba, S, Aburatani, H, Yachie, N, Siksnys, V, Ohmori, T, Hoshino, A, Nureki, O. | | Deposit date: | 2023-04-13 | | Release date: | 2023-09-27 | | Method: | ELECTRON MICROSCOPY (2.91 Å) | | Cite: | Minimal and most efficient genome editing Cas enzyme
To Be Published
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6FJW
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6QXT
 | | Cas1-Cas2-Csn2-DNA dimer complex from the Type II-A CRISPR-Cas system | | Descriptor: | CALCIUM ION, CRISPR-associated endonuclease Cas1, CRISPR-associated endoribonuclease Cas2, ... | | Authors: | Wilkinson, M, Drabavicius, G, Silanskas, A, Gasiunas, G, Siksnys, V, Wigley, D.B. | | Deposit date: | 2019-03-08 | | Release date: | 2019-05-08 | | Last modified: | 2024-05-15 | | Method: | ELECTRON MICROSCOPY (8.9 Å) | | Cite: | Structure of the DNA-Bound Spacer Capture Complex of a Type II CRISPR-Cas System.
Mol.Cell, 75, 2019
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6QY3
 | | Segment of the Cas1-Cas2-Csn2-DNA filament complex from the Type II-A CRISPR-Cas system | | Descriptor: | CALCIUM ION, CRISPR-associated endonuclease Cas1, CRISPR-associated endoribonuclease Cas2, ... | | Authors: | Wilkinson, M, Drabavicius, G, Silanskas, A, Gasiunas, G, Siksnys, V, Wigley, D.B. | | Deposit date: | 2019-03-08 | | Release date: | 2019-05-08 | | Last modified: | 2024-05-15 | | Method: | ELECTRON MICROSCOPY (9.1 Å) | | Cite: | Structure of the DNA-Bound Spacer Capture Complex of a Type II CRISPR-Cas System.
Mol.Cell, 75, 2019
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6QXF
 | | Cas1-Cas2-Csn2-DNA complex from the Type II-A CRISPR-Cas system | | Descriptor: | CALCIUM ION, CRISPR-associated endonuclease Cas1, CRISPR-associated endoribonuclease Cas2, ... | | Authors: | Wilkinson, M, Drabavicius, G, Silanskas, A, Gasiunas, G, Siksnys, V, Wigley, D.B. | | Deposit date: | 2019-03-07 | | Release date: | 2019-05-08 | | Last modified: | 2024-05-15 | | Method: | ELECTRON MICROSCOPY (3.6 Å) | | Cite: | Structure of the DNA-Bound Spacer Capture Complex of a Type II CRISPR-Cas System.
Mol.Cell, 75, 2019
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3SSE
 | | DNA binding domain of restriction endonuclease bound to DNA | | Descriptor: | 5-methylcytosine-specific restriction enzyme B, DNA (5'-D(*A*GP*CP*TP*AP*CP*CP*GP*GP*TP*CP*TP*C)-3'), DNA (5'-D(*T*GP*AP*GP*AP*CP*CP*GP*GP*TP*AP*GP*C)-3') | | Authors: | Sukackaite, R, Grazulis, S, Siksnys, V. | | Deposit date: | 2011-07-08 | | Release date: | 2012-05-23 | | Last modified: | 2024-02-28 | | Method: | X-RAY DIFFRACTION (2.7 Å) | | Cite: | The recognition domain of the methyl-specific endonuclease McrBC flips out 5-methylcytosine.
Nucleic Acids Res., 40, 2012
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3SSD
 | | DNA binding domain of restriction endonuclease bound to DNA | | Descriptor: | 5-methylcytosine-specific restriction enzyme B, DNA (5'-D(*A*GP*CP*TP*AP*CP*CP*GP*GP*TP*CP*TP*C)-3'), DNA (5'-D(*T*GP*AP*GP*AP*(5CM)P*CP*GP*GP*TP*AP*GP*C)-3') | | Authors: | Sukackaite, R, Grazulis, S, Siksnys, V. | | Deposit date: | 2011-07-08 | | Release date: | 2012-05-23 | | Last modified: | 2024-02-28 | | Method: | X-RAY DIFFRACTION (2.2 Å) | | Cite: | The recognition domain of the methyl-specific endonuclease McrBC flips out 5-methylcytosine.
Nucleic Acids Res., 40, 2012
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3SSC
 | | DNA binding domain of restriction endonuclease bound to DNA | | Descriptor: | 5-methylcytosine-specific restriction enzyme B, DNA (5'-D(*AP*GP*CP*TP*AP*(5CM)P*CP*GP*GP*TP*CP*TP*C)-3'), DNA (5'-D(*TP*GP*AP*GP*AP*(5CM)P*CP*GP*GP*TP*AP*GP*C)-3') | | Authors: | Sukackaite, R, Grazulis, S, Siksnys, V. | | Deposit date: | 2011-07-08 | | Release date: | 2012-05-23 | | Last modified: | 2024-02-28 | | Method: | X-RAY DIFFRACTION (2.1 Å) | | Cite: | The recognition domain of the methyl-specific endonuclease McrBC flips out 5-methylcytosine.
Nucleic Acids Res., 40, 2012
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7AE9
 | | Crystal structure of mono-AMPylated HEPN(R46E) toxin in complex with MNT antitoxin | | Descriptor: | 2',3'-DIDEOXYADENOSINE-5'-MONOPHOSPHATE, HEPN toxin, MNT ANTITOXIN | | Authors: | Tamulaitiene, G, Sasnauskas, G, Songailiene, I, Juozapaitis, J, Siksnys, V. | | Deposit date: | 2020-09-17 | | Release date: | 2020-12-30 | | Last modified: | 2024-01-31 | | Method: | X-RAY DIFFRACTION (2.9 Å) | | Cite: | HEPN-MNT Toxin-Antitoxin System: The HEPN Ribonuclease Is Neutralized by OligoAMPylation.
Mol.Cell, 80, 2020
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7AE8
 | | Crystal structure of HEPN(R102A) toxin | | Descriptor: | ACETATE ION, HEPN toxin | | Authors: | Tamulaitiene, G, Sasnauskas, G, Songailiene, I, Juozapaitis, J, Siksnys, V. | | Deposit date: | 2020-09-17 | | Release date: | 2020-12-30 | | Last modified: | 2024-01-31 | | Method: | X-RAY DIFFRACTION (2 Å) | | Cite: | HEPN-MNT Toxin-Antitoxin System: The HEPN Ribonuclease Is Neutralized by OligoAMPylation.
Mol.Cell, 80, 2020
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