5DWA

Crystal structure of pre-specific restriction endonuclease AgeI-DNA complex

Summary for 5DWA

• Entry DOI: 10.2210/pdb5dwa/pdb
• Descriptor: Type-2 restriction enzyme AgeI, DNA (5'-D(*TP*CP*GP*AP*CP*CP*GP*GP*TP*CP*G*)-3') (3 entities in total)
• Functional Keywords: restriction endonuclease, pd-(d/e)xk nuclease, protein-dna complex, hydrolase
• Biological source: Thalassobius gelatinovorus; More
• Total number of polymer chains: 4
• Total formula weight: 67855.41

Authors

Tamulaitiene, G.,Jovaisaite, V.,Grazulis, S.,Siksnys, V. (deposition date: 2015-09-22, release date: 2016-10-05, Last modification date: 2019-07-17)

• Primary citation: Tamulaitiene, G.,Jovaisaite, V.,Tamulaitis, G.,Songailiene, I.,Manakova, E.,Zaremba, M.,Grazulis, S.,Xu, S.Y.,Siksnys, V.; Restriction endonuclease AgeI is a monomer which dimerizes to cleave DNA.; Nucleic Acids Res., 45:3547-3558, 2017; PubMed: 28039325; DOI: 10.1093/nar/gkw1310

Experimental method

X-RAY DIFFRACTION (1.5 Å)