6EKO

Crystal structure of Type IIP restriction endonuclease PfoI with cognate DNA

Summary for 6EKO

• Entry DOI: 10.2210/pdb6eko/pdb
• Related: 6EK1
• Descriptor: Restriction endonuclease PfoI, DNA (5'-D(*CP*GP*CP*TP*CP*CP*CP*GP*GP*AP*GP*CP*GP*T)-3'), CALCIUM ION, ... (4 entities in total)
• Functional Keywords: restriction endonuclease, pd-(d/e)xk nuclease, hydrolase
• Biological source: Pseudomonas fluorescens; More
• Total number of polymer chains: 4
• Total formula weight: 79851.21

Authors

Tamulaitiene, G.,Manakova, E.,Jovaisaite, V.,Grazulis, S.,Siksnys, V. (deposition date: 2017-09-26, release date: 2018-10-10, Last modification date: 2019-04-24)

• Primary citation: Tamulaitiene, G.,Manakova, E.,Jovaisaite, V.,Tamulaitis, G.,Grazulis, S.,Bochtler, M.,Siksnys, V.; Unique mechanism of target recognition by PfoI restriction endonuclease of the CCGG-family.; Nucleic Acids Res., 47:997-1010, 2019; PubMed: 30445642; DOI: 10.1093/nar/gky1137

Experimental method

X-RAY DIFFRACTION (2.284 Å)