6EKO
Crystal structure of Type IIP restriction endonuclease PfoI with cognate DNA
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I911-3 |
Synchrotron site | MAX II |
Beamline | I911-3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-12-03 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.96112,0.98010 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.279, 91.663, 152.742 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.960 - 2.284 |
R-factor | 0.2094 |
Rwork | 0.205 |
R-free | 0.24780 |
Structure solution method | MAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.085 |
Data reduction software | XDS (VERSION Jun 17, 2015 BUILT=20150617) |
Data scaling software | SCALA (CCP4 6.2: Scala version 3.3.20 : 23/06/11) |
Phasing software | Auto-Rickshaw |
Refinement software | PHENIX (1.8.3_1479) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.960 | 2.410 |
High resolution limit [Å] | 2.280 | 2.280 |
Rmerge | 0.198 | |
Rmeas | 0.216 | |
Rpim | 0.058 | 0.442 |
Number of reflections | 36181 | 4683 |
<I/σ(I)> | 11.6 | 1.8 |
Completeness [%] | 98.5 | 89.8 |
Redundancy | 13.5 | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 292 | Reservoir: 20% PEG8000, 0.1M TrisHCl pH8.5, LiCl 0.2M and glycerol 10%. Protein-DNA complex concentration 5.3 mg/ml |