3AXI
 
 | | Crystal structure of isomaltase in complex with maltose | | Descriptor: | CALCIUM ION, Oligo-1,6-glucosidase IMA1, alpha-D-glucopyranose | | Authors: | Yamamoto, K, Miyake, H, Kusunoki, M, Osaki, S. | | Deposit date: | 2011-04-06 | | Release date: | 2011-10-05 | | Last modified: | 2023-11-01 | | Method: | X-RAY DIFFRACTION (1.4 Å) | | Cite: | Steric hindrance by 2 amino acid residues determines the substrate specificity of isomaltase from Saccharomyces cerevisiae
J.Biosci.Bioeng., 112, 2011
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8WUT
 | | SpCas9-MMLV RT-pegRNA-target DNA complex (initiation) | | Descriptor: | CRISPR-associated endonuclease Cas9/Csn1, DNA (5'-D(*TP*GP*AP*TP*GP*GP*CP*AP*GP*AP*GP*TP*AP*CP*TP*AP*G)-3'), DNA (51-MER), ... | | Authors: | Shuto, Y, Nakagawa, R, Hoki, M, Omura, S.N, Hirano, H, Itoh, Y, Nureki, O. | | Deposit date: | 2023-10-21 | | Release date: | 2024-06-05 | | Last modified: | 2024-09-11 | | Method: | ELECTRON MICROSCOPY (3 Å) | | Cite: | Structural basis for pegRNA-guided reverse transcription by a prime editor.
Nature, 631, 2024
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8WUS
 | | SpCas9-MMLV RT-pegRNA-target DNA complex (termination) | | Descriptor: | CRISPR-associated endonuclease Cas9/Csn1, DNA (40-MER), DNA (5'-D(*TP*GP*AP*TP*GP*GP*CP*AP*GP*AP*GP*TP*AP*CP*TP*AP*G)-3'), ... | | Authors: | Shuto, Y, Nakagawa, R, Hoki, M, Omura, S.N, Hirano, H, Itoh, Y, Nureki, O. | | Deposit date: | 2023-10-21 | | Release date: | 2024-06-05 | | Last modified: | 2024-09-11 | | Method: | ELECTRON MICROSCOPY (2.9 Å) | | Cite: | Structural basis for pegRNA-guided reverse transcription by a prime editor.
Nature, 631, 2024
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8WUU
 | | SpCas9-pegRNA-target DNA complex (pre-initiation) | | Descriptor: | CRISPR-associated endonuclease Cas9/Csn1, DNA (34-MER), DNA (5'-D(*TP*GP*AP*TP*GP*GP*CP*AP*GP*AP*GP*TP*AP*CP*TP*AP*G)-3'), ... | | Authors: | Shuto, Y, Nakagawa, R, Hoki, M, Omura, S.N, Hirano, H, Itoh, Y, Nureki, O. | | Deposit date: | 2023-10-21 | | Release date: | 2024-06-05 | | Last modified: | 2024-09-11 | | Method: | ELECTRON MICROSCOPY (3.2 Å) | | Cite: | Structural basis for pegRNA-guided reverse transcription by a prime editor.
Nature, 631, 2024
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8WUV
 | | SpCas9-MMLV RT-pegRNA-target DNA complex (elongation 16-nt) | | Descriptor: | CRISPR-associated endonuclease Cas9/Csn1, DNA (5'-D(*TP*GP*AP*TP*GP*GP*CP*AP*GP*AP*GP*TP*AP*CP*TP*AP*G)-3'), DNA (50-MER), ... | | Authors: | Shuto, Y, Nakagawa, R, Hoki, M, Omura, S.N, Hirano, H, Itoh, Y, Nureki, O. | | Deposit date: | 2023-10-21 | | Release date: | 2024-06-05 | | Last modified: | 2024-09-11 | | Method: | ELECTRON MICROSCOPY (3 Å) | | Cite: | Structural basis for pegRNA-guided reverse transcription by a prime editor.
Nature, 631, 2024
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8YFY
 | | CRYSTAL STRUCTURE OF THE EST1 H274D MUTANT AT PH 4.2 | | Descriptor: | Carboxylesterase, octyl beta-D-glucopyranoside | | Authors: | Unno, H, Oshima, Y, Nishino, T, Nakayama, T, Kusunoki, M. | | Deposit date: | 2024-02-26 | | Release date: | 2024-07-10 | | Last modified: | 2024-08-28 | | Method: | X-RAY DIFFRACTION (1.45 Å) | | Cite: | Lowering pH optimum of activity of SshEstI, a slightly alkaliphilic archaeal esterase of the hormone-sensitive lipase family.
J.Biosci.Bioeng., 138, 2024
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8YFZ
 | | CRYSTAL STRUCTURE OF THE EST1 H274E MUTANT AT PH 4.2 | | Descriptor: | Carboxylesterase, octyl beta-D-glucopyranoside | | Authors: | Unno, H, Oshima, Y, Nishino, T, Nakayama, T, Kusunoki, M. | | Deposit date: | 2024-02-26 | | Release date: | 2024-07-10 | | Method: | X-RAY DIFFRACTION (1.5 Å) | | Cite: | Lowering pH optimum of activity of SshEstI, a slightly alkaliphilic archaeal esterase of the hormone-sensitive lipase family.
J.Biosci.Bioeng., 2024
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8YGJ
 | | SpCas9-MMLV RT-pegRNA-target DNA complex (elongation 28-nt) | | Descriptor: | CRISPR-associated endonuclease Cas9/Csn1, DNA (5'-D(P*TP*GP*AP*TP*GP*GP*CP*AP*GP*AP*GP*TP*AP*CP*TP*AP*G)-3'), DNA (51-MER), ... | | Authors: | Shuto, Y, Nakagawa, R, Hoki, M, Omura, S.N, Hirano, H, Itoh, Y, Nureki, O. | | Deposit date: | 2024-02-26 | | Release date: | 2024-06-05 | | Last modified: | 2024-09-11 | | Method: | ELECTRON MICROSCOPY (3.2 Å) | | Cite: | Structural basis for pegRNA-guided reverse transcription by a prime editor.
Nature, 631, 2024
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3TKK
 | | Crystal Structure Analysis of a recombinant predicted acetamidase/ formamidase from the thermophile thermoanaerobacter tengcongensis | | Descriptor: | CALCIUM ION, Predicted acetamidase/formamidase, ZINC ION | | Authors: | Qian, M, Huang, Q, Wu, G, Lai, L, Tang, Y, Pei, J, Kusunoki, M. | | Deposit date: | 2011-08-26 | | Release date: | 2011-11-16 | | Last modified: | 2012-02-08 | | Method: | X-RAY DIFFRACTION (1.99 Å) | | Cite: | Crystal Structure Analysis of a Recombinant Predicted Acetamidase/Formamidase from the Thermophile Thermoanaerobacter tengcongensis.
PROTEIN J., 31, 2012
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1G6K
 | | Crystal structure of glucose dehydrogenase mutant E96A complexed with NAD+ | | Descriptor: | GLUCOSE 1-DEHYDROGENASE, NICOTINAMIDE-ADENINE-DINUCLEOTIDE | | Authors: | Yamamoto, K, Kurisu, G, Kusunoki, M, Tabata, S, Urabe, I, Osaki, S. | | Deposit date: | 2000-11-06 | | Release date: | 2003-08-12 | | Last modified: | 2023-10-25 | | Method: | X-RAY DIFFRACTION (2 Å) | | Cite: | Structural analysis of stability-increasing mutants of glucose dehydrogenase
To be Published
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1GEE
 | | Crystal structure of glucose dehydrogenase mutant Q252L complexed with NAD+ | | Descriptor: | GLUCOSE 1-DEHYDROGENASE, NICOTINAMIDE-ADENINE-DINUCLEOTIDE | | Authors: | Yamamoto, K, Kurisu, G, Kusunoki, M, Tabata, S, Urabe, I, Osaki, S. | | Deposit date: | 2000-11-07 | | Release date: | 2003-08-12 | | Last modified: | 2023-10-25 | | Method: | X-RAY DIFFRACTION (1.6 Å) | | Cite: | Structural analysis of stability-increasing mutants of glucose dehydrogenase
To be Published
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3WMH
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1IWP
 | | Glycerol Dehydratase-cyanocobalamin Complex of Klebsiella pneumoniae | | Descriptor: | COBALAMIN, Glycerol Dehydratase Alpha subunit, Glycerol Dehydratase Beta subunit, ... | | Authors: | Yamanishi, M, Yunoki, M, Tobimatsu, T, Toraya, T. | | Deposit date: | 2002-05-28 | | Release date: | 2002-10-02 | | Last modified: | 2023-10-25 | | Method: | X-RAY DIFFRACTION (2.1 Å) | | Cite: | The crystal structure of coenzyme B12-dependent glycerol dehydratase in complex with cobalamin and propane-1,2-diol.
Eur.J.Biochem., 269, 2002
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1J10
 | | beta-amylase from Bacillus cereus var. mycoides in complex with GGX | | Descriptor: | Beta-amylase, CALCIUM ION, alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-xylopyranose, ... | | Authors: | Oyama, T, Miyake, H, Kusunoki, M, Nitta, Y. | | Deposit date: | 2002-11-25 | | Release date: | 2003-06-17 | | Last modified: | 2023-10-25 | | Method: | X-RAY DIFFRACTION (2.1 Å) | | Cite: | Crystal Structures of beta-Amylase from Bacillus cereus var. mycoides in Complexes with Substrate Analogs and Affinity-Labeling Reagents
J.BIOCHEM.(TOKYO), 133, 2003
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1J11
 | | beta-amylase from Bacillus cereus var. mycoides in complex with alpha-EPG | | Descriptor: | (2R)-oxiran-2-ylmethyl alpha-D-glucopyranoside, Beta-amylase, CALCIUM ION | | Authors: | Oyama, T, Miyake, H, Kusunoki, M, Nitta, Y. | | Deposit date: | 2002-11-25 | | Release date: | 2003-06-17 | | Last modified: | 2023-10-25 | | Method: | X-RAY DIFFRACTION (2 Å) | | Cite: | Crystal Structures of beta-Amylase from Bacillus cereus var. mycoides in Complexes with Substrate Analogs and Affinity-Labeling Reagents
J.BIOCHEM.(TOKYO), 133, 2003
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3WJ2
 | | Crystal structure of ESTFA (FE-lacking apo form) | | Descriptor: | Carboxylesterase | | Authors: | Ohara, K, Unno, H, Oshima, Y, Furukawa, K, Fujino, N, Hirooka, K, Hemmi, H, Takahashi, S, Nishino, T, Kusunoki, M, Nakayama, T. | | Deposit date: | 2013-10-03 | | Release date: | 2014-07-30 | | Last modified: | 2024-03-20 | | Method: | X-RAY DIFFRACTION (1.61 Å) | | Cite: | Structural insights into the low pH adaptation of a unique carboxylesterase from Ferroplasma: altering the pH optima of two carboxylesterases.
J.Biol.Chem., 289, 2014
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3WJ1
 | | Crystal structure of SSHESTI | | Descriptor: | Carboxylesterase, octyl beta-D-glucopyranoside | | Authors: | Ohara, K, Unno, H, Oshima, Y, Furukawa, K, Fujino, N, Hirooka, K, Hemmi, H, Takahashi, S, Nishino, T, Kusunoki, M, Nakayama, T. | | Deposit date: | 2013-10-03 | | Release date: | 2014-07-30 | | Last modified: | 2020-07-29 | | Method: | X-RAY DIFFRACTION (1.5 Å) | | Cite: | Structural insights into the low pH adaptation of a unique carboxylesterase from Ferroplasma: altering the pH optima of two carboxylesterases.
J.Biol.Chem., 289, 2014
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1GCO
 | | CRYSTAL STRUCTURE OF GLUCOSE DEHYDROGENASE COMPLEXED WITH NAD+ | | Descriptor: | GLUCOSE DEHYDROGENASE, NICOTINAMIDE-ADENINE-DINUCLEOTIDE | | Authors: | Yamamoto, K, Kurisu, G, Kusunoki, M, Tabata, S, Urabe, I, Osaki, S. | | Deposit date: | 2000-08-07 | | Release date: | 2001-02-28 | | Last modified: | 2023-12-27 | | Method: | X-RAY DIFFRACTION (1.7 Å) | | Cite: | Crystal structure of glucose dehydrogenase from Bacillus megaterium IWG3 at 1.7 A resolution.
J.Biochem., 129, 2001
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1GEG
 | | CRYATAL STRUCTURE ANALYSIS OF MESO-2,3-BUTANEDIOL DEHYDROGENASE | | Descriptor: | ACETOIN REDUCTASE, BETA-MERCAPTOETHANOL, MAGNESIUM ION, ... | | Authors: | Otagiri, M, Kurisu, G, Ui, S, Kusunoki, M. | | Deposit date: | 2000-11-10 | | Release date: | 2001-02-28 | | Last modified: | 2023-12-27 | | Method: | X-RAY DIFFRACTION (1.7 Å) | | Cite: | Crystal structure of meso-2,3-butanediol dehydrogenase in a complex with NAD+ and inhibitor mercaptoethanol at 1.7 A resolution for understanding of chiral substrate recognition mechanisms.
J.Biochem., 129, 2001
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3WYE
 | | Crystal Structure of chimeric engineered (2S,3S)-butanediol dehydrogenase complexed with NAD+ | | Descriptor: | Diacetyl reductase [(S)-acetoin forming],L-2,3-butanediol dehydrogenase,Diacetyl reductase [(S)-acetoin forming],L-2,3-butanediol dehydrogenase,Diacetyl reductase [(S)-acetoin forming],L-2,3-butanediol dehydrogenase,Diacetyl reductase [(S)-acetoin forming], NICOTINAMIDE-ADENINE-DINUCLEOTIDE | | Authors: | Shimegi, T, Oyama, T, Kusunoki, M, Ui, S. | | Deposit date: | 2014-08-26 | | Release date: | 2015-08-26 | | Last modified: | 2024-03-20 | | Method: | X-RAY DIFFRACTION (1.58 Å) | | Cite: | Crystal Structure of chimeric engineered (2S,3S)-butanediol dehydrogenase complexed with NAD+
To be Published
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1GAQ
 | | CRYSTAL STRUCTURE OF THE COMPLEX BETWEEN FERREDOXIN AND FERREDOXIN-NADP+ REDUCTASE | | Descriptor: | FE2/S2 (INORGANIC) CLUSTER, FERREDOXIN I, FERREDOXIN-NADP+ REDUCTASE, ... | | Authors: | Kurisu, G, Kusunoki, M, Hase, T. | | Deposit date: | 2000-05-08 | | Release date: | 2001-02-07 | | Last modified: | 2023-12-27 | | Method: | X-RAY DIFFRACTION (2.59 Å) | | Cite: | Structure of the electron transfer complex between ferredoxin and ferredoxin-NADP(+) reductase.
Nat.Struct.Biol., 8, 2001
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1GAW
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7T71
 | | Crystal Structure of Mevalonate 3,5-Bisphosphate Decarboxylase from Picrophilus Torridus | | Descriptor: | Mevalonate 3,5-bisphosphate decarboxylase, OLEIC ACID | | Authors: | Vinokur, J.M, Sawaya, M.R, Cascio, D, Collazo, M, Bowie, J.U. | | Deposit date: | 2021-12-14 | | Release date: | 2021-12-22 | | Last modified: | 2023-10-25 | | Method: | X-RAY DIFFRACTION (2.19 Å) | | Cite: | Crystal structure of mevalonate 3,5-bisphosphate decarboxylase reveals insight into the evolution of decarboxylases in the mevalonate metabolic pathways.
J.Biol.Chem., 298, 2022
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5ZU2
 | | Effect of mutation (R554A) on FAD modification in Aspergillus oryzae RIB40formate oxidase | | Descriptor: | (4S)-2-METHYL-2,4-PENTANEDIOL, ACETATE ION, FLAVIN-ADENINE DINUCLEOTIDE, ... | | Authors: | Mikami, B, Uchida, H, Doubayashi, D. | | Deposit date: | 2018-05-06 | | Release date: | 2019-05-22 | | Last modified: | 2024-03-27 | | Method: | X-RAY DIFFRACTION (2.441 Å) | | Cite: | The microenvironment surrounding FAD mediates its conversion to 8-formyl-FAD in Aspergillus oryzae RIB40 formate oxidase.
J.Biochem., 166, 2019
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5ZU3
 | | Effect of mutation (R554K) on FAD modification in Aspergillus oryzae RIB40formate oxidase | | Descriptor: | (4S)-2-METHYL-2,4-PENTANEDIOL, ACETATE ION, Formate oxidase, ... | | Authors: | Mikami, B, Uchida, H, Doubayashi, D. | | Deposit date: | 2018-05-06 | | Release date: | 2019-05-22 | | Last modified: | 2024-03-27 | | Method: | X-RAY DIFFRACTION (2.4 Å) | | Cite: | The microenvironment surrounding FAD mediates its conversion to 8-formyl-FAD in Aspergillus oryzae RIB40 formate oxidase.
J.Biochem., 166, 2019
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