8YGJ

SpCas9-MMLV RT-pegRNA-target DNA complex (elongation 28-nt)

Summary for 8YGJ

• Entry DOI: 10.2210/pdb8ygj/pdb
• EMDB information: 39253
• Descriptor: RNA (137-MER), DNA (51-MER), DNA (5'-D(P*TP*GP*AP*TP*GP*GP*CP*AP*GP*AP*GP*TP*AP*CP*TP*AP*G)-3'), ... (6 entities in total)
• Functional Keywords: crispr-cas, rna binding protein-dna-rna complex, rna binding protein/dna/rna
• Biological source: Streptococcus pyogenes; More
• Total number of polymer chains: 6
• Total formula weight: 298370.82

Authors

Shuto, Y.,Nakagawa, R.,Hoki, M.,Omura, S.N.,Hirano, H.,Itoh, Y.,Nureki, O. (deposition date: 2024-02-26, release date: 2024-06-05, Last modification date: 2024-09-11)

• Primary citation: Shuto, Y.,Nakagawa, R.,Zhu, S.,Hoki, M.,Omura, S.N.,Hirano, H.,Itoh, Y.,Zhang, F.,Nureki, O.; Structural basis for pegRNA-guided reverse transcription by a prime editor.; Nature, 631:224-231, 2024; PubMed: 38811740; DOI: 10.1038/s41586-024-07497-8

Experimental method

ELECTRON MICROSCOPY (3.2 Å)