8YGJ
SpCas9-MMLV RT-pegRNA-target DNA complex (elongation 28-nt)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (B) | RNA (137-MER) | polymer | 137 | 44316.4 | 1 | Streptococcus pyogenes | ||
| 2 | B (C) | DNA (51-MER) | polymer | 51 | 15636.0 | 1 | Streptococcus pyogenes | ||
| 3 | C (D) | DNA (5'-D(P*TP*GP*AP*TP*GP*GP*CP*AP*GP*AP*GP*TP*AP*CP*TP*AP*G)-3') | polymer | 17 | 5291.4 | 1 | Streptococcus pyogenes | ||
| 4 | D (E) | Gag-Pol polyprotein | polymer | 496 | 55656.2 | 1 | UniProt (Q8UN00) Pfam (PF00078) Pfam (PF17919) | Moloney murine leukemia virus | |
| 5 | E (F) | DNA (62-MER) | polymer | 62 | 19013.1 | 1 | Streptococcus pyogenes | ||
| 6 | F (A) | CRISPR-associated endonuclease Cas9/Csn1 | polymer | 1367 | 158457.6 | 1 | UniProt (Q99ZW2) Pfam (PF16593) Pfam (PF16592) Pfam (PF13395) Pfam (PF22702) Pfam (PF16595) | Streptococcus pyogenes serotype M1 |
Sequence modifications
E: 1 - 496 (UniProt: Q8UN00)
A: 2 - 1368 (UniProt: Q99ZW2)
| PDB | External Database | Details |
|---|---|---|
| Asn 200 | Asp 859 | engineered mutation |
| Asn 249 | Asp 908 | engineered mutation |
| Lys 306 | Thr 965 | engineered mutation |
| Phe 313 | Trp 972 | engineered mutation |
| Pro 330 | Thr 989 | engineered mutation |
| Asn 335 | Asp 994 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Ala 10 | Asp 10 | engineered mutation |
| Ala 840 | His 840 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 298370.8 | |
| All* | Total formula weight | 298370.8 |
