8WUU

SpCas9-pegRNA-target DNA complex (pre-initiation)

This is a non-PDB format compatible entry.

Summary for 8WUU

• Entry DOI: 10.2210/pdb8wuu/pdb
• EMDB information: 37860
• Descriptor: CRISPR-associated endonuclease Cas9/Csn1, DNA (51-MER), DNA (5'-D(*TP*GP*AP*TP*GP*GP*CP*AP*GP*AP*GP*TP*AP*CP*TP*AP*G)-3'), ... (5 entities in total)
• Functional Keywords: crispr-cas, rna binding protein-rna-dna complex, rna binding protein/rna/dna
• Biological source: Streptococcus pyogenes serotype M1; More
• Total number of polymer chains: 5
• Total formula weight: 234148.18

Authors

Shuto, Y.,Nakagawa, R.,Hoki, M.,Omura, S.N.,Hirano, H.,Itoh, Y.,Nureki, O. (deposition date: 2023-10-21, release date: 2024-06-05, Last modification date: 2024-06-12)

• Primary citation: Shuto, Y.,Nakagawa, R.,Zhu, S.,Hoki, M.,Omura, S.N.,Hirano, H.,Itoh, Y.,Zhang, F.,Nureki, O.; Structural basis for pegRNA-guided reverse transcription by a prime editor.; Nature, 2024; PubMed: 38811740; DOI: 10.1038/s41586-024-07497-8

Experimental method

ELECTRON MICROSCOPY (3.2 Å)