3J5M
Cryo-EM structure of the BG505 SOSIP.664 HIV-1 Env trimer with 3 PGV04 Fabs
Summary for 3J5M
| Entry DOI | 10.2210/pdb3j5m/pdb |
| Related | 4NCO |
| EMDB information | 5779 |
| Descriptor | BG505 SOSIP gp120, BG505 SOSIP gp41, PGV04 light chain, ... (4 entities in total) |
| Functional Keywords | hiv-1 trimeric spike, gp140, sosip, broadly neutralizing antibody, pgv04, env, envelope glycoprotein, viral protein-immune system complex, viral protein/immune system |
| Biological source | Human immunodeficiency virus 1 More |
| Total number of polymer chains | 12 |
| Total formula weight | 318913.28 |
| Authors | Lyumkis, D.,Julien, J.-P.,Wilson, I.A.,Ward, A.B. (deposition date: 2013-10-26, release date: 2013-11-13, Last modification date: 2024-11-27) |
| Primary citation | Lyumkis, D.,Julien, J.P.,de Val, N.,Cupo, A.,Potter, C.S.,Klasse, P.J.,Burton, D.R.,Sanders, R.W.,Moore, J.P.,Carragher, B.,Wilson, I.A.,Ward, A.B. Cryo-EM structure of a fully glycosylated soluble cleaved HIV-1 envelope trimer. Science, 342:1484-1490, 2013 Cited by PubMed Abstract: The HIV-1 envelope glycoprotein (Env) trimer contains the receptor binding sites and membrane fusion machinery that introduce the viral genome into the host cell. As the only target for broadly neutralizing antibodies (bnAbs), Env is a focus for rational vaccine design. We present a cryo-electron microscopy reconstruction and structural model of a cleaved, soluble Env trimer (termed BG505 SOSIP.664 gp140) in complex with a CD4 binding site (CD4bs) bnAb, PGV04, at 5.8 angstrom resolution. The structure reveals the spatial arrangement of Env components, including the V1/V2, V3, HR1, and HR2 domains, as well as shielding glycans. The structure also provides insights into trimer assembly, gp120-gp41 interactions, and the CD4bs epitope cluster for bnAbs, which covers a more extensive area and defines a more complex site of vulnerability than previously described. PubMed: 24179160DOI: 10.1126/science.1245627 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (5.8 Å) |
Structure validation
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