3J5M
Cryo-EM structure of the BG505 SOSIP.664 HIV-1 Env trimer with 3 PGV04 Fabs
EM specimen
| Vitrification | Yes |
| Instrument | HOMEMADE PLUNGER |
| Method | Specimen was prepared for cryo-EM by applying 3 microliters of sample to a freshly plasma cleaned holey carbon C-flat grid (Protochips, Inc.), allowing the sample to adsorb to the grid for 30 seconds, followed by blotting with a small piece of filter paper and plunge-freezing into liquid ethane using a manual cryo-plunger in an ambient environment (4 C). |
EM Data Collection
| Microscope | FEI TECNAI F20 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
| Number of Images | 6355 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 5.8 |
| Software | FREALIGN,Xmipp |
| Symmetry | C3 |
