9VL2

Structure of CD33 complexed with its mAb-10C8

Summary for 9VL2

Entry DOI	10.2210/pdb9vl2/pdb
Descriptor	Myeloid cell surface antigen CD33, light chain of Fab-10C8, heavy chain of Fab-10C8, ... (5 entities in total)
Functional Keywords	cd33 siglec-3 hbv infection immune checkpoint, immune system
Biological source	Homo sapiens (human) More
Total number of polymer chains	6
Total formula weight	142847.45
Authors	Yeh, Y.H.,Sung, P.S.,Hsieh, S.L.,Hsiao, C.D. (deposition date: 2025-06-24, release date: 2026-05-27, Last modification date: 2026-06-10)
Primary citation	Yeh, Y.H.,Lin, M.G.,Sung, P.S.,Hsieh, S.L.,Hsiao, C.D. Crystal structure of the CD33/Fab-10C8 complex elucidates the mechanism of antibody antagonism in HBV-induced immunosuppression. J.Biomed.Sci., 33:-, 2026 Cited by PubMed Abstract: Hepatitis B virus (HBV) infection persists through immune evasion strategies, including engagement of the inhibitory receptor CD33 (Siglec-3) by α2,6-linked sialoglycans on HBsAg. This interaction induces ITIM phosphorylation and SHP-1/2 recruitment, dampening myeloid cell activation. The monoclonal antibody 10C8 has been identified as a potent antagonist of CD33, but the structural basis for its inhibitory activity remains unclear. PubMed: 42215976 DOI: 10.1186/s12929-026-01248-9 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (3.18 Å)

Structure validation