9VL2
Structure of CD33 complexed with its mAb-10C8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE TPS 05A |
| Synchrotron site | NSRRC |
| Beamline | TPS 05A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2021-10-08 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 0.9998 |
| Spacegroup name | P 1 |
| Unit cell lengths | 71.408, 71.156, 86.765 |
| Unit cell angles | 95.89, 94.16, 111.23 |
Refinement procedure
| Resolution | 28.350 - 3.180 |
| R-factor | 0.2088 |
| Rwork | 0.205 |
| R-free | 0.25230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.527 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (("2.0_5885": ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.400 |
| High resolution limit [Å] | 3.180 | 3.370 |
| Rmerge | 0.047 | 0.095 |
| Number of reflections | 23442 | 881 |
| <I/σ(I)> | 19.5 | |
| Completeness [%] | 92.7 | |
| Redundancy | 1.9 | |
| CC(1/2) | 0.970 | 0.860 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293.15 | Tris-HCl pH 8.5, 15% PEG 4000 |
