9VDJ

Serial synchrotron crystallography structure of a ba3-type cytochrome c oxidase using a goniometer-compatible chip-based platform

Summary for 9VDJ

• Entry DOI: 10.2210/pdb9vdj/pdb
• Descriptor: Cytochrome c oxidase subunit 1, Cytochrome c oxidase subunit 2, Cytochrome c oxidase polypeptide 2A, ... (9 entities in total)
• Functional Keywords: proton-pump, bioenergetics, complex iv, serial crystallography, lipidic cubic phase, membrane protein
• Biological source: Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8); More
• Total number of polymer chains: 3
• Total formula weight: 92967.22

Authors

Kabbinale, A.,Branden, G.,Neutze, R.,Ghosh, S. (deposition date: 2025-06-08, release date: 2026-03-25, Last modification date: 2026-04-22)

Primary citation

Ghosh, S.,Banacore, A.,Norder, P.,Bjelcic, M.,Kabbinale, A.,Nileshwar, P.,Wehlander, G.,de Sanctis, D.,Basu, S.,Orlans, J.,Vallejos, A.,Chavas, L.M.G.,Neutze, R.,Branden, G.
A user-friendly goniometer-compatible fixed-target platform for macromolecular crystallography at synchrotrons.
J.Appl.Crystallogr., 59:303-315, 2026

PubMed Abstract: Fixed-target platforms provide convenient support for microcrystals during serial X-ray crystallography studies using synchrotron radiation. Here, we describe a simple user-friendly 3D-printed support where the crystals are sandwiched between two layers of thin X-ray-transparent membrane resulting in very low scattering background. The platform is compatible with magnetic mounting onto the standard goniometer of macromolecular crystallography beamlines. Our design utilizes a 96-well frame that facilitates hanging-drop experiments directly on the membrane using conventional crystallization plates, thereby eliminating multiple pipetting and crystal handling steps. Crystals can be enclosed in a sandwich and packed into 'cassettes', preventing the risk of the sample drying out during room-temperature transportation to synchrotron sources. The versatility of the platform is demonstrated by five structures solved using different crystallization and data-collection strategies. Lysozyme single-crystal rotational crystallography at room temperature is shown, as well as microcrystal serial data collection under cryogenic conditions. On-chip microcrystallization is illustrated by use of a photosynthetic reaction center as an example. Finally, serial crystallography data collection at room temperature from microcrystals of the membrane protein cytochrome oxidase crystallized in lipidic cubic phase is presented.

PubMed: 41959872
DOI: 10.1107/S1600576725011513

Experimental method

X-RAY DIFFRACTION (2.3 Å)