9GBU

Nitratidesulfovibrio vulgaris [FeFe]-hydrogenase variant with both subunits linked by a 13 amino acid linker peptide derived from a group A1 type [FeFe]-hydrogenase of Solobacterium moorei

Summary for 9GBU

• Entry DOI: 10.2210/pdb9gbu/pdb
• Descriptor: Periplasmic [Fe] hydrogenase large subunit,Periplasmic [Fe] hydrogenase small subunit, (4S)-2-METHYL-2,4-PENTANEDIOL, dicarbonyl[bis(cyanide-kappaC)]-mu-(iminodimethanethiolatato-1kappaS:2kappaS)-mu-(oxomethylidene)diiron(2+), ... (5 entities in total)
• Functional Keywords: [fefe] hydrogenase, iron-sulfur cluster, metalloenzyme, hydrogen production, fusion protein, oxidoreductase
• Biological source: Nitratidesulfovibrio vulgaris; More
• Total number of polymer chains: 2
• Total formula weight: 111658.66

Authors

Bikbaev, K.,Jaenecke, J.,Winkler, M.,Span, I. (deposition date: 2024-07-31, release date: 2025-08-13, Last modification date: 2026-02-25)

• Primary citation: Winkler, M.H.,Jaenecke, J.,Bikbaev, K.,Bronold, J.,Yadav, S.,Apfel, U.P.,Birrell, J.A.,Span, I.,Plumere, N.,Leger, C.,Malagnini, M.; Subunit fusion unlocks rapid in vitro maturation for slowly activating heterodimeric [FeFe]-hydrogenases; Chem Sci, 2026; DOI: 10.1039/D5SC07299A

Experimental method

X-RAY DIFFRACTION (1.785 Å)