9GBU
Desulfovibrio desulfuricans [FeFe]-hydrogenase variant with both subunits linked by a 13 amino acid linker peptide derived from a group A1 type [FeFe]-hydrogenase of Solobacterium moorei
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0008901 | molecular_function | ferredoxin hydrogenase activity |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0042597 | cellular_component | periplasmic space |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0051536 | molecular_function | iron-sulfur cluster binding |
| A | 0051539 | molecular_function | 4 iron, 4 sulfur cluster binding |
| B | 0008901 | molecular_function | ferredoxin hydrogenase activity |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0042597 | cellular_component | periplasmic space |
| B | 0046872 | molecular_function | metal ion binding |
| B | 0051536 | molecular_function | iron-sulfur cluster binding |
| B | 0051539 | molecular_function | 4 iron, 4 sulfur cluster binding |
Functional Information from PROSITE/UniProt
| site_id | PS00198 |
| Number of Residues | 12 |
| Details | 4FE4S_FER_1 4Fe-4S ferredoxin-type iron-sulfur binding region signature. CiGCDtCSqYCP |
| Chain | Residue | Details |
| A | CYS35-PRO46 | |
| A | CYS66-PRO77 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 62 |
| Details | Domain: {"description":"4Fe-4S ferredoxin-type 1","evidences":[{"source":"PROSITE-ProRule","id":"PRU00711","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 54 |
| Details | Domain: {"description":"4Fe-4S ferredoxin-type 2","evidences":[{"source":"PROSITE-ProRule","id":"PRU00711","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 24 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"10368269","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1HFE","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 20 |
| Details | Region: {"description":"Disordered","evidences":[{"source":"SAM","id":"MobiDB-lite","evidenceCode":"ECO:0000256"}]} |
| Chain | Residue | Details |
Catalytic Information from CSA
| site_id | MCSA1 |
| Number of Residues | 8 |
| Details | M-CSA 127 |
| Chain | Residue | Details |
| A | GLU156 | proton acceptor, proton donor, proton relay |
| A | GLU159 | proton acceptor, proton donor, proton relay |
| A | CYS178 | proton acceptor, proton donor, proton relay |
| A | SER198 | proton acceptor, proton donor, proton relay |
| A | LYS237 | proton acceptor, proton donor, proton relay |
| A | GLU240 | proton acceptor, proton donor, proton relay |
| A | GLU245 | proton acceptor, proton donor, proton relay |
| A | CYS382 | metal ligand |
| site_id | MCSA2 |
| Number of Residues | 8 |
| Details | M-CSA 127 |
| Chain | Residue | Details |
| B | GLU156 | proton acceptor, proton donor, proton relay |
| B | GLU159 | proton acceptor, proton donor, proton relay |
| B | CYS178 | proton acceptor, proton donor, proton relay |
| B | SER198 | proton acceptor, proton donor, proton relay |
| B | LYS237 | proton acceptor, proton donor, proton relay |
| B | GLU240 | proton acceptor, proton donor, proton relay |
| B | GLU245 | proton acceptor, proton donor, proton relay |
| B | CYS382 | metal ligand |
