9GBU

Desulfovibrio desulfuricans [FeFe]-hydrogenase variant with both subunits linked by a 13 amino acid linker peptide derived from a group A1 type [FeFe]-hydrogenase of Solobacterium moorei

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Periplasmic [Fe] hydrogenase large subunit,Periplasmic [Fe] hydrogenase small subunit	polymer	490	54064.9	2	UniProt (P07598) UniProt (P07603) Pfam (PF00037) Pfam (PF02906) Pfam (PF02256)	Desulfovibrio desulfuricans	Fe hydrogenlyase,Fe hydrogenlyase small chain
2	C, D, E, F, K... (A, B)	(4S)-2-METHYL-2,4-PENTANEDIOL	non-polymer		118.2	6	Chemie (MPD)
3	G, N (A, B)	dicarbonyl[bis(cyanide-kappaC)]-mu-(iminodimethanethiolatato-1kappaS:2kappaS)-mu-(oxomethylidene)diiron(2+)	non-polymer		355.0	2	Chemie (402)
4	H, I, J, M, O... (A, B)	IRON/SULFUR CLUSTER	non-polymer		351.6	6	Chemie (SF4)
5	Q, R (A, B)	water	water		18.0	221	Chemie (HOH)

Sequence modifications

A, B: 1 - 389 (UniProt: P07598)

PDB	External Database	Details
Arg 390	-	linker
His 391	-	linker
Lys 392	-	linker
Leu 393	-	linker
Pro 394	-	linker
Gln 395	-	linker
Val 396	-	linker
Lys 397	-	linker
Ala 398	-	linker
Ala 399	-	linker
Lys 400	-	linker
Glu 401	-	linker
Ser 402	-	linker

A, B: 403 - 480 (UniProt: P07603)

PDB	External Database	Details
Ser 481	-	expression tag
Ala 482	-	expression tag
Trp 483	-	expression tag
Ser 484	-	expression tag
His 485	-	expression tag
Pro 486	-	expression tag
Gln 487	-	expression tag
Phe 488	-	expression tag
Glu 489	-	expression tag
Lys 490	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	108129.9
Non-Polymers*	Number of molecules	14
	Total formula weight	3528.8
All*	Total formula weight	111658.7

*Water molecules are not included.