9CAI

High-resolution C. elegans 80S ribosome structure - class 1

This is a non-PDB format compatible entry.

Summary for 9CAI

• Entry DOI: 10.2210/pdb9cai/pdb
• Related: 9BH5
• EMDB information: 45392
• Descriptor: Small ribosomal subunit protein uS2, 5.8S rRNA, 18S rRNA, ... (81 entities in total)
• Functional Keywords: ribosome, endogenous purified 80s ribosome
• Biological source: Caenorhabditis elegans; More
• Total number of polymer chains: 79
• Total formula weight: 3163653.10

Authors

Sehgal, E.,Serrao, V.H.B.,Arribere, J. (deposition date: 2024-06-17, release date: 2024-09-11, Last modification date: 2024-10-30)

Primary citation

Sehgal, E.,Wohlenberg, C.,Soukup, E.M.,Viscardi, M.J.,Serrao, V.H.B.,Arribere, J.A.
High-resolution reconstruction of a C. elegans ribosome sheds light on evolutionary dynamics and tissue specificity.
Rna, 30:1513-1528, 2024

PubMed Abstract: Caenorhabditis elegans is an important model organism for human health and disease, with foundational contributions to the understanding of gene expression and tissue patterning in animals. An invaluable tool in modern gene expression research is the presence of a high-resolution ribosome structure, though no such structure exists for C. elegans. Here we present a high-resolution single-particle cryogenic electron microscopy (cryoEM) reconstruction and molecular model of a C. elegans ribosome, revealing a significantly streamlined animal ribosome. Many facets of ribosome structure are conserved in C. elegans, including overall ribosomal architecture and the mechanism of cycloheximide, while other facets such as expansion segments and eL28 are rapidly evolving. We identify uL5 and uL23 as two instances of tissue-specific ribosomal protein paralog expression conserved in Caenorhabditis, suggesting that C. elegans ribosomes vary across tissues. The C. elegans ribosome structure will provide a basis for future structural, biochemical, and genetic studies of translation in this important animal system.

PubMed: 39209556
DOI: 10.1261/rna.080103.124

Experimental method

ELECTRON MICROSCOPY (2.59 Å)