9B37

Open state of kainate receptor GluK2 in complex with agonist glutamate and positive allosteric modulator BPAM344 bound to one concanavalin A dimer. Composite map.

Summary for 9B37

• Entry DOI: 10.2210/pdb9b37/pdb
• EMDB information: 44130
• Descriptor: Glutamate receptor ionotropic, kainate 2, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 4-cyclopropyl-7-fluoro-3,4-dihydro-2H-1,2,4-benzothiadiazine 1,1-dioxide, ... (17 entities in total)
• Functional Keywords: kainate receptor, gluk2, positive allosteric modulator, bpam344, open, concanavalin a, cona, glutamate, membrane protein
• Biological source: Rattus norvegicus (Norway rat); More
• Total number of polymer chains: 6
• Total formula weight: 497410.32

Authors

Nadezhdin, K.D.,Gangwar, S.P.,Sobolevsky, A.I. (deposition date: 2024-03-18, release date: 2024-05-22, Last modification date: 2024-11-13)

Primary citation

Gangwar, S.P.,Yelshanskaya, M.V.,Nadezhdin, K.D.,Yen, L.Y.,Newton, T.P.,Aktolun, M.,Kurnikova, M.G.,Sobolevsky, A.I.
Kainate receptor channel opening and gating mechanism.
Nature, 630:762-768, 2024

PubMed Abstract: Kainate receptors, a subclass of ionotropic glutamate receptors, are tetrameric ligand-gated ion channels that mediate excitatory neurotransmission. Kainate receptors modulate neuronal circuits and synaptic plasticity during the development and function of the central nervous system and are implicated in various neurological and psychiatric diseases, including epilepsy, depression, schizophrenia, anxiety and autism. Although structures of kainate receptor domains and subunit assemblies are available, the mechanism of kainate receptor gating remains poorly understood. Here we present cryo-electron microscopy structures of the kainate receptor GluK2 in the presence of the agonist glutamate and the positive allosteric modulators lectin concanavalin A and BPAM344. Concanavalin A and BPAM344 inhibit kainate receptor desensitization and prolong activation by acting as a spacer between the amino-terminal and ligand-binding domains and a stabilizer of the ligand-binding domain dimer interface, respectively. Channel opening involves the kinking of all four pore-forming M3 helices. Our structures reveal the molecular basis of kainate receptor gating, which could guide the development of drugs for treatment of neurological disorders.

PubMed: 38778115
DOI: 10.1038/s41586-024-07475-0

Experimental method

ELECTRON MICROSCOPY (6.66 Å)