9B37

Open state of kainate receptor GluK2 in complex with agonist glutamate and positive allosteric modulator BPAM344 bound to one concanavalin A dimer. Composite map.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Glutamate receptor ionotropic, kainate 2	polymer	912	102976.6	4	UniProt (P42260) Pfam (PF01094) Pfam (PF10613) Pfam (PF00060) In PDB	Rattus norvegicus (Norway rat)	GluK2,Glutamate receptor 6,GluR-6,GluR6
2	E, F	Concanavalin A	polymer	237	25622.4	2	UniProt (C0HJY1) Pfam (PF00139) In PDB	Canavalia ensiformis (jack bean)	ConV
3	b, e, G, g, J...	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	15	In PDB GlyTouCan (G42666HT)
4	d, H, Y	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	3	In PDB GlyTouCan (G15407YE)
5	I, W	beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1479.3	2	In PDB GlyTouCan (G89993FE)
6	N, O	beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1276.2	2	In PDB GlyTouCan (G28663KH)
7	P	Cyclic 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-6)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)]alpha-D-mannopyranose	branched		1114.0	1	In PDB
8	f, S	alpha-D-mannopyranose-(1-6)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		748.7	2	In PDB GlyTouCan (G22573RC)
9	a	beta-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		748.7	1	In PDB GlyTouCan (G31886NL)
10	c	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1317.2	1	In PDB GlyTouCan (G39213VZ)
11	A, D, B, C	4-cyclopropyl-7-fluoro-3,4-dihydro-2H-1,2,4-benzothiadiazine 1,1-dioxide	non-polymer		242.3	4	Chemie (2J9)
12	C, A, D, B	GLUTAMIC ACID	non-polymer		147.1	4	Chemie (GLU)
13	C, D, A, B	(2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate	non-polymer		760.1	14	Chemie (POV)
14	C, A, B	CHOLESTEROL	non-polymer		386.7	8	Chemie (CLR)
15	C, B	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	2	Chemie (NAG)
16	E, F	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
17	E, F	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)

Sequence modifications

A, B, C, D: 1 - 908 (UniProt: P42260)

PDB	External Database	Details
Val 567	Ile 567	conflict
Cys 571	Tyr 571	conflict
Leu 909	-	expression tag
Val 910	-	expression tag
Pro 911	-	expression tag
Arg 912	-	expression tag

E, F: 1 - 237 (UniProt: C0HJY1)

PDB	External Database	Details
Asp 58	Gly 58	conflict
Ala 70	Gly 70	conflict
Met 129	Val 129	conflict
Glu 192	Asp 192	conflict

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	463151.1
Branched	Number of molecules	27
	Total formula weight	18313.9
Non-Polymers*	Number of molecules	36
	Total formula weight	15945.3
All*	Total formula weight	497410.3

*Water molecules are not included.